5.1H11

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$40.00
SKU: 5.1H11
View product citations for antibody 5.1H11 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: NCAM
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Blau, H.M. / Walsh, F.S.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Stanford University
Positive Tested Species Reactivity: Human
Depositors Notes: mAb 5.1H11 is specific for NCAM in skeletal muscle and reacts only with human.
Antigen Molecular Weight: 94.5 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: NCAM
Immunogen: Fused myoblasts
Alternate Gene Names: NCAM1, CD56
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3xG3 Ag8
Epitope Location or Sequence:
Uniprot ID: P13591 
Immunogen Sequence:
Entrez Gene ID: 4684 
Additional Characterization:
Antibody Registry ID: AB_531857 
Additional Information: 5.1H11 has been used extensively to purify human myoblasts using the MACS system. See FACS citations. RRID:AB_531857
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
5.1H11 was deposited to the DSHB by Blau, H.M. / Walsh, F.S. (DSHB Hybridoma Product 5.1H11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

21 References

  • Initial Publication
  • IF References
  • IHC References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication

    Nature
    Ritter MA
    Nature 289.5793 (1981 Jan 1): 60-4.

    IF References

    Nature
    Ritter MA
    Nature 289.5793 (1981 Jan 1): 60-4.

    The Journal of cell biology
    Blau HM
    The Journal of cell biology 102.1 (1986 Jan): 124-30.

    Nature
    Blau HM
    Nature 337.6207 (1989 Feb 9): 570-3.

    FASEB journal : official publication of the Federation of American Societies for Experimental Biology
    Parise G
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 26.6 (2012 Jun): 2509-21.

    Journal of cell science
    Blau HM
    Journal of cell science 122.Pt 7 (2009 Apr 1): 1045-53.

    Proceedings of the National Academy of Sciences of the United States of America
    Blau HM
    Proceedings of the National Academy of Sciences of the United States of America 104.11 (2007 Mar 13): 4395-400.

    Journal of cell science
    Fisher AG
    Journal of cell science 119.Pt 10 (2006 May 15): 2065-72.

    American journal of physiology. Cell physiology
    Doumit ME
    American journal of physiology. Cell physiology 282.4 (2002 Apr): C899-906.

    IHC References

    The Journal of physiology
    Goldman YE
    The Journal of physiology 432. (1991 Jan): 639-80.

    FACS References

    Biochemical and biophysical research communications
    Fulle S
    Biochemical and biophysical research communications 473.2 (2016 Apr 29): 462-70.

    BMC biotechnology
    Rome S
    BMC biotechnology 16. (2016 Apr 2): 32.

    International journal of obesity (2005)
    Bourlier V
    International journal of obesity (2005) 40.3 (2016 Mar): 497-506.

    PloS one
    Rossi FM
    PloS one 6.10 (2011): e26381.

    PloS one
    Smith SR
    PloS one 6.7 (2011): e21068.

    PloS one
    Schäfer BW
    PloS one 6.5 (2011): e19506.

    FASEB journal : official publication of the Federation of American Societies for Experimental Biology
    Blau HM
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 23.5 (2009 May): 1431-40.

    Diabetologia
    Vidal H
    Diabetologia 51.3 (2008 Mar): 512-21.

    Nature biotechnology
    Studer L
    Nature biotechnology 25.12 (2007 Dec): 1468-75.

    Diabetologia
    Vidal H
    Diabetologia 49.5 (2006 May): 990-9.

    Yonsei medical journal
    Kim J
    Yonsei medical journal 47.2 (2006 Apr 30): 179-83.

    Epitope Map References

    International journal of cancer
    Walsh FS
    International journal of cancer 44.4 (1989 Oct 15): 573-8.

    All References

    The Journal of physiology
    Goldman YE
    The Journal of physiology 432. (1991 Jan): 639-80.

    Nature
    Ritter MA
    Nature 289.5793 (1981 Jan 1): 60-4.

    The Journal of cell biology
    Blau HM
    The Journal of cell biology 102.1 (1986 Jan): 124-30.

    Nature
    Blau HM
    Nature 337.6207 (1989 Feb 9): 570-3.

    FASEB journal : official publication of the Federation of American Societies for Experimental Biology
    Parise G
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 26.6 (2012 Jun): 2509-21.

    Journal of cell science
    Blau HM
    Journal of cell science 122.Pt 7 (2009 Apr 1): 1045-53.

    Proceedings of the National Academy of Sciences of the United States of America
    Blau HM
    Proceedings of the National Academy of Sciences of the United States of America 104.11 (2007 Mar 13): 4395-400.

    Journal of cell science
    Fisher AG
    Journal of cell science 119.Pt 10 (2006 May 15): 2065-72.

    American journal of physiology. Cell physiology
    Doumit ME
    American journal of physiology. Cell physiology 282.4 (2002 Apr): C899-906.

    Biochemical and biophysical research communications
    Fulle S
    Biochemical and biophysical research communications 473.2 (2016 Apr 29): 462-70.

    BMC biotechnology
    Rome S
    BMC biotechnology 16. (2016 Apr 2): 32.

    International journal of obesity (2005)
    Bourlier V
    International journal of obesity (2005) 40.3 (2016 Mar): 497-506.

    PloS one
    Rossi FM
    PloS one 6.10 (2011): e26381.

    PloS one
    Smith SR
    PloS one 6.7 (2011): e21068.

    PloS one
    Schäfer BW
    PloS one 6.5 (2011): e19506.

    FASEB journal : official publication of the Federation of American Societies for Experimental Biology
    Blau HM
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 23.5 (2009 May): 1431-40.

    Diabetologia
    Vidal H
    Diabetologia 51.3 (2008 Mar): 512-21.

    Nature biotechnology
    Studer L
    Nature biotechnology 25.12 (2007 Dec): 1468-75.

    Diabetologia
    Vidal H
    Diabetologia 49.5 (2006 May): 990-9.

    Yonsei medical journal
    Kim J
    Yonsei medical journal 47.2 (2006 Apr 30): 179-83.

    International journal of cancer
    Walsh FS
    International journal of cancer 44.4 (1989 Oct 15): 573-8.

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