5.1H11

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$40.00
SKU: 5.1H11
View product citations for antibody 5.1H11 on CiteAb

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Available: 119

DSHB Data Sheet

Catalog Fields

Antigen: NCAM
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Blau, H.M. / Walsh, F.S.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Stanford University
Positive Tested Species Reactivity: Human
Depositors Notes: mAb 5.1H11 is specific for NCAM in skeletal muscle and reacts only with human.
Antigen Molecular Weight: 94.5 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: NCAM
Immunogen: Fused myoblasts
Alternate Gene Names: NCAM1, CD56
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3xG3 Ag8
Epitope Location or Sequence:
Uniprot ID: P13591 
Immunogen Sequence:
Entrez Gene ID: 4684 
Additional Characterization:
Antibody Registry ID: AB_531857 
Additional Information: 5.1H11 has been used extensively to purify human myoblasts using the MACS system. See FACS citations. RRID:AB_531857
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
5.1H11 was deposited to the DSHB by Blau, H.M. / Walsh, F.S. (DSHB Hybridoma Product 5.1H11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

21 References

  • Initial Publication
  • IF References
  • IHC References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication

    Surface antigen differentiation during human myogenesis in culture.
    Ritter MA
    Nature 289.5793 (1981 Jan 1): 60-4.

    IF References

    Surface antigen differentiation during human myogenesis in culture.
    Ritter MA
    Nature 289.5793 (1981 Jan 1): 60-4.

    Expression of muscle genes in heterokaryons depends on gene dosage.
    Blau HM
    The Journal of cell biology 102.1 (1986 Jan): 124-30.

    Localization of muscle gene products in nuclear domains.
    Blau HM
    Nature 337.6207 (1989 Feb 9): 570-3.

    Myostatin is associated with age-related human muscle stem cell dysfunction.
    Parise G
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 26.6 (2012 Jun): 2509-21.

    Reprogramming to a muscle fate by fusion recapitulates differentiation.
    Blau HM
    Journal of cell science 122.Pt 7 (2009 Apr 1): 1045-53.

    Active tissue-specific DNA demethylation conferred by somatic cell nuclei in stable heterokaryons.
    Blau HM
    Proceedings of the National Academy of Sciences of the United States of America 104.11 (2007 Mar 13): 4395-400.

    Acquisition and extinction of gene expression programs are separable events in heterokaryon reprogramming.
    Fisher AG
    Journal of cell science 119.Pt 10 (2006 May 15): 2065-72.

    Satellite cell proliferation and differentiation during postnatal growth of porcine skeletal muscle.
    Doumit ME
    American journal of physiology. Cell physiology 282.4 (2002 Apr): C899-906.

    IHC References
    FACS References

    Myomir dysregulation and reactive oxygen species in aged human satellite cells.
    Fulle S
    Biochemical and biophysical research communications 473.2 (2016 Apr 29): 462-70.

    Depleting extracellular vesicles from fetal bovine serum alters proliferation and differentiation of skeletal muscle cells in vitro.
    Rome S
    BMC biotechnology 16. (2016 Apr 2): 32.

    Adipogenic progenitors from obese human skeletal muscle give rise to functional white adipocytes that contribute to insulin resistance.
    Bourlier V
    International journal of obesity (2005) 40.3 (2016 Mar): 497-506.

    Targeted cell fusion facilitates stable heterokaryon generation in vitro and in vivo.
    Rossi FM
    PloS one 6.10 (2011): e26381.

    Remodeling lipid metabolism and improving insulin responsiveness in human primary myotubes.
    Smith SR
    PloS one 6.7 (2011): e21068.

    CD133 positive embryonal rhabdomyosarcoma stem-like cell population is enriched in rhabdospheres.
    Schäfer BW
    PloS one 6.5 (2011): e19506.

    Nuclear reprogramming in heterokaryons is rapid, extensive, and bidirectional.
    Blau HM
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 23.5 (2009 May): 1431-40.

    Isoform-specific defects of insulin stimulation of Akt/protein kinase B (PKB) in skeletal muscle cells from type 2 diabetic patients.
    Vidal H
    Diabetologia 51.3 (2008 Mar): 512-21.

    Isolation and directed differentiation of neural crest stem cells derived from human embryonic stem cells.
    Studer L
    Nature biotechnology 25.12 (2007 Dec): 1468-75.

    Activation of liver X receptors promotes lipid accumulation but does not alter insulin action in human skeletal muscle cells.
    Vidal H
    Diabetologia 49.5 (2006 May): 990-9.

    A comparative study of magnetic-activated cell sorting, cytotoxicity and preplating for the purification of human myoblasts.
    Kim J
    Yonsei medical journal 47.2 (2006 Apr 30): 179-83.

    Epitope Map References
    All References

    Cross-bridge kinetics in the presence of MgADP investigated by photolysis of caged ATP in rabbit psoas muscle fibres.
    Goldman YE
    The Journal of physiology 432. (1991 Jan): 639-80.

    Surface antigen differentiation during human myogenesis in culture.
    Ritter MA
    Nature 289.5793 (1981 Jan 1): 60-4.

    Expression of muscle genes in heterokaryons depends on gene dosage.
    Blau HM
    The Journal of cell biology 102.1 (1986 Jan): 124-30.

    Localization of muscle gene products in nuclear domains.
    Blau HM
    Nature 337.6207 (1989 Feb 9): 570-3.

    Myostatin is associated with age-related human muscle stem cell dysfunction.
    Parise G
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 26.6 (2012 Jun): 2509-21.

    Reprogramming to a muscle fate by fusion recapitulates differentiation.
    Blau HM
    Journal of cell science 122.Pt 7 (2009 Apr 1): 1045-53.

    Active tissue-specific DNA demethylation conferred by somatic cell nuclei in stable heterokaryons.
    Blau HM
    Proceedings of the National Academy of Sciences of the United States of America 104.11 (2007 Mar 13): 4395-400.

    Acquisition and extinction of gene expression programs are separable events in heterokaryon reprogramming.
    Fisher AG
    Journal of cell science 119.Pt 10 (2006 May 15): 2065-72.

    Satellite cell proliferation and differentiation during postnatal growth of porcine skeletal muscle.
    Doumit ME
    American journal of physiology. Cell physiology 282.4 (2002 Apr): C899-906.

    Myomir dysregulation and reactive oxygen species in aged human satellite cells.
    Fulle S
    Biochemical and biophysical research communications 473.2 (2016 Apr 29): 462-70.

    Depleting extracellular vesicles from fetal bovine serum alters proliferation and differentiation of skeletal muscle cells in vitro.
    Rome S
    BMC biotechnology 16. (2016 Apr 2): 32.

    Adipogenic progenitors from obese human skeletal muscle give rise to functional white adipocytes that contribute to insulin resistance.
    Bourlier V
    International journal of obesity (2005) 40.3 (2016 Mar): 497-506.

    Targeted cell fusion facilitates stable heterokaryon generation in vitro and in vivo.
    Rossi FM
    PloS one 6.10 (2011): e26381.

    Remodeling lipid metabolism and improving insulin responsiveness in human primary myotubes.
    Smith SR
    PloS one 6.7 (2011): e21068.

    CD133 positive embryonal rhabdomyosarcoma stem-like cell population is enriched in rhabdospheres.
    Schäfer BW
    PloS one 6.5 (2011): e19506.

    Nuclear reprogramming in heterokaryons is rapid, extensive, and bidirectional.
    Blau HM
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 23.5 (2009 May): 1431-40.

    Isoform-specific defects of insulin stimulation of Akt/protein kinase B (PKB) in skeletal muscle cells from type 2 diabetic patients.
    Vidal H
    Diabetologia 51.3 (2008 Mar): 512-21.

    Isolation and directed differentiation of neural crest stem cells derived from human embryonic stem cells.
    Studer L
    Nature biotechnology 25.12 (2007 Dec): 1468-75.

    Activation of liver X receptors promotes lipid accumulation but does not alter insulin action in human skeletal muscle cells.
    Vidal H
    Diabetologia 49.5 (2006 May): 990-9.

    A comparative study of magnetic-activated cell sorting, cytotoxicity and preplating for the purification of human myoblasts.
    Kim J
    Yonsei medical journal 47.2 (2006 Apr 30): 179-83.

    Neural cell adhesion molecule (NCAM) is the antigen recognized by monoclonal antibodies of similar specificity in small-cell lung carcinoma and neuroblastoma.
    Walsh FS
    International journal of cancer 44.4 (1989 Oct 15): 573-8.

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