5.1H11
Catalog Fields
Antigen: NCAM
Available to For-Profits: Yes
Antigen Species: Human
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1, kappa light chain
Depositor: Blau, H.M. / Walsh, F.S.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Human
Depositors Institution: Stanford University
Antigen Molecular Weight: 94.5 kDa
Depositors Notes: mAb 5.1H11 is specific for NCAM in skeletal muscle and reacts only with human.
Immunogen: Fused myoblasts
Gene: NCAM
Alternate Antibody Name:
Alternate Gene Names: NCAM1, CD56
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: P3xG3 Ag8
Epitope Mapped: Yes
Uniprot ID: P13591
Epitope Location or Sequence:
Entrez Gene ID: 4684
Immunogen Sequence:
Antibody Registry ID: AB_531857
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry
Additional Information: 5.1H11 has been used extensively to purify human myoblasts using the MACS system. See FACS citations. RRID:AB_531857
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
5.1H11 was deposited to the DSHB by Blau, H.M. / Walsh, F.S. (DSHB Hybridoma Product 5.1H11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
21 References
Initial Publication
Surface antigen differentiation during human myogenesis in culture.
Ritter MA
Nature 289.5793 (1981 Jan 1): 60-4.