A4.74

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$40.00
SKU: A4.74
View product citations for antibody A4.74 on CiteAb

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Available: 90

DSHB Data Sheet

Catalog Fields

Antigen: Myosin heavy chain (human fast fibers)
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Blau, H.M.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Baxter Lab for Stem Cell Biology, Stanford University
Positive Tested Species Reactivity: Bovine, C. elegans, Horse, Human, Llama, Porcine, Quail, Rodent
Depositors Notes: Fusion: 1983. Recognizes all fast fibers in human. In rat, high affinity for IIA fibers, low affinity for IIX/D fibers, and doesn't stain IIB.
Antigen Molecular Weight: 220kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: MYH2
Immunogen: partially purified myosin (pyrophosphate extracted) from human adult (22 year old) skeletal muscle
Alternate Gene Names: BM3; MYH2A; MYPOP; MYHSA2; MYHas8; MyHC-2A; MyHC-IIa
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: 653
Epitope Location or Sequence:
Uniprot ID: Q9UKX2 
Immunogen Sequence:
Entrez Gene ID: 4620 
Additional Characterization:
Antibody Registry ID: AB_528383 
Additional Information: stains MyHCIIa [PMID 7687223]. This antibody may recognize more than one myosin isoform depending on the tissue, developmental stage, or species. The gene, Uniprot ID and gene ID associated with this mAb are not exclusive.
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
A4.74 was deposited to the DSHB by Blau, H.M. (DSHB Hybridoma Product A4.74)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

33 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • All References
  • Initial Publication

    Cell
    Blau HM
    Cell 52.4 (1988 Feb 26): 503-13.

    IF References

    Cell
    Blau HM
    Cell 52.4 (1988 Feb 26): 503-13.

    Journal of anatomy
    Domellöf FP
    Journal of anatomy 226.6 (2015 Jun): 542-8.

    Muscle & nerve
    Svensson MB
    Muscle & nerve 52.5 (2015 Nov): 812-7.

    Cytokine
    Hidalgo J
    Cytokine 74.1 (2015 Jul): 145-51.

    Muscle & nerve
    Piercy RJ
    Muscle & nerve 53.4 (2016 Apr): 583-92.

    Respiratory physiology & neurobiology
    Mantilla CB
    Respiratory physiology & neurobiology 180.1 (2012 Jan 15): 88-96.

    Meat science
    Geay Y
    Meat science 55.1 (2000 May): 67-78.

    Developmental biology
    Blau HM
    Developmental biology 158.1 (1993 Jul): 183-99.

    Investigative ophthalmology & visual science
    Kaminski HJ
    Investigative ophthalmology & visual science 51.12 (2010 Dec): 6355-63.

    Developmental biology
    Thompson W
    Developmental biology 356.2 (2011 Aug 15): 432-44.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Charbonnier F
    The Journal of neuroscience : the official journal of the Society for Neuroscience 35.34 (2015 Aug 26): 12063-79.

    Developmental biology
    Blau HM
    Developmental biology 262.1 (2003 Oct 1): 64-74.

    WB References

    Developmental biology
    Blau HM
    Developmental biology 158.1 (1993 Jul): 183-99.

    Cell
    Blau HM
    Cell 52.4 (1988 Feb 26): 503-13.

    Journal of applied physiology (Bethesda, Md. : 1985)
    Murphy RM
    Journal of applied physiology (Bethesda, Md. : 1985) 110.3 (2011 Mar): 820-5.

    Investigative ophthalmology & visual science
    Thornell LE
    Investigative ophthalmology & visual science 41.7 (2000 Jun): 1608-16.

    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
    Pedrosa-Domellöf F
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 50.2 (2002 Feb): 171-83.

    Poultry science
    Lee K
    Poultry science 95.8 (2016 Aug 1): 1912-7.

    Meat science
    Geay Y
    Meat science 55.1 (2000 May): 67-78.

    The Annals of otology, rhinology, and laryngology
    Flint PW
    The Annals of otology, rhinology, and laryngology 108.5 (1999 May): 509-15.

    Circulation
    Mancini D
    Circulation 95.4 (1997 Feb 18): 910-6.

    The Laryngoscope
    Flint PW
    The Laryngoscope 109.9 (1999 Sep): 1521-4.

    Journal of molecular and cellular cardiology
    Levine S
    Journal of molecular and cellular cardiology 28.12 (1996 Dec): 2537-41.

    IHC References

    Skeletal muscle
    Rüegg MA
    Skeletal muscle 3.1 (2013 Mar 6): 6.

    Developmental biology
    Patel K
    Developmental biology 343.1-2 (2010 Jul 1): 51-62.

    Journal of morphology
    Rivero JL
    Journal of morphology 262.2 (2004 Nov): 667-81.

    Investigative ophthalmology & visual science
    Thornell LE
    Investigative ophthalmology & visual science 41.7 (2000 Jun): 1608-16.

    The Anatomical record
    Black AC Jr
    The Anatomical record 264.1 (2001 Sep 1): 51-62.

    Muscle & nerve
    Hildebrand C
    Muscle & nerve 24.9 (2001 Sep): 1202-12.

    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
    Pedrosa-Domellöf F
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 50.2 (2002 Feb): 171-83.

    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
    Pedrosa-Domellöf F
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 51.2 (2003 Feb): 175-86.

    Current topics in microbiology and immunology
    Young HE
    Current topics in microbiology and immunology 280. (2004): 71-109.

    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology
    Black AC Jr
    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 276.1 (2004 Jan): 75-102.

    Journal of anatomy
    Rivero JL
    Journal of anatomy 221.2 (2012 Aug): 151-63.

    Cell
    Blau HM
    Cell 68.4 (1992 Feb 21): 659-71.

    Muscle & nerve
    May CA
    Muscle & nerve 52.1 (2015 Jul): 55-62.

    Journal of muscle research and cell motility
    Hughes SM
    Journal of muscle research and cell motility 21.2 (2000 Feb): 101-13.

    Histochemistry and cell biology
    Eriksson PO
    Histochemistry and cell biology 139.6 (2013 Jun): 895-907.

    ELISA References

    Developmental biology
    Blau HM
    Developmental biology 158.1 (1993 Jul): 183-99.

    Cell
    Blau HM
    Cell 52.4 (1988 Feb 26): 503-13.

    All References

    Skeletal muscle
    Rüegg MA
    Skeletal muscle 3.1 (2013 Mar 6): 6.

    Developmental biology
    Patel K
    Developmental biology 343.1-2 (2010 Jul 1): 51-62.

    Journal of morphology
    Rivero JL
    Journal of morphology 262.2 (2004 Nov): 667-81.

    Investigative ophthalmology & visual science
    Thornell LE
    Investigative ophthalmology & visual science 41.7 (2000 Jun): 1608-16.

    The Anatomical record
    Black AC Jr
    The Anatomical record 264.1 (2001 Sep 1): 51-62.

    Muscle & nerve
    Hildebrand C
    Muscle & nerve 24.9 (2001 Sep): 1202-12.

    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
    Pedrosa-Domellöf F
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 50.2 (2002 Feb): 171-83.

    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
    Pedrosa-Domellöf F
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 51.2 (2003 Feb): 175-86.

    Current topics in microbiology and immunology
    Young HE
    Current topics in microbiology and immunology 280. (2004): 71-109.

    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology
    Black AC Jr
    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 276.1 (2004 Jan): 75-102.

    Journal of anatomy
    Rivero JL
    Journal of anatomy 221.2 (2012 Aug): 151-63.

    Cell
    Blau HM
    Cell 68.4 (1992 Feb 21): 659-71.

    Muscle & nerve
    May CA
    Muscle & nerve 52.1 (2015 Jul): 55-62.

    Journal of muscle research and cell motility
    Hughes SM
    Journal of muscle research and cell motility 21.2 (2000 Feb): 101-13.

    Histochemistry and cell biology
    Eriksson PO
    Histochemistry and cell biology 139.6 (2013 Jun): 895-907.

    Cell
    Blau HM
    Cell 52.4 (1988 Feb 26): 503-13.

    Journal of anatomy
    Domellöf FP
    Journal of anatomy 226.6 (2015 Jun): 542-8.

    Muscle & nerve
    Svensson MB
    Muscle & nerve 52.5 (2015 Nov): 812-7.

    Cytokine
    Hidalgo J
    Cytokine 74.1 (2015 Jul): 145-51.

    Muscle & nerve
    Piercy RJ
    Muscle & nerve 53.4 (2016 Apr): 583-92.

    Respiratory physiology & neurobiology
    Mantilla CB
    Respiratory physiology & neurobiology 180.1 (2012 Jan 15): 88-96.

    Meat science
    Geay Y
    Meat science 55.1 (2000 May): 67-78.

    Developmental biology
    Blau HM
    Developmental biology 158.1 (1993 Jul): 183-99.

    Investigative ophthalmology & visual science
    Kaminski HJ
    Investigative ophthalmology & visual science 51.12 (2010 Dec): 6355-63.

    Developmental biology
    Thompson W
    Developmental biology 356.2 (2011 Aug 15): 432-44.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Charbonnier F
    The Journal of neuroscience : the official journal of the Society for Neuroscience 35.34 (2015 Aug 26): 12063-79.

    Developmental biology
    Blau HM
    Developmental biology 262.1 (2003 Oct 1): 64-74.

    Journal of applied physiology (Bethesda, Md. : 1985)
    Murphy RM
    Journal of applied physiology (Bethesda, Md. : 1985) 110.3 (2011 Mar): 820-5.

    Poultry science
    Lee K
    Poultry science 95.8 (2016 Aug 1): 1912-7.

    The Annals of otology, rhinology, and laryngology
    Flint PW
    The Annals of otology, rhinology, and laryngology 108.5 (1999 May): 509-15.

    Circulation
    Mancini D
    Circulation 95.4 (1997 Feb 18): 910-6.

    The Laryngoscope
    Flint PW
    The Laryngoscope 109.9 (1999 Sep): 1521-4.

    Journal of molecular and cellular cardiology
    Levine S
    Journal of molecular and cellular cardiology 28.12 (1996 Dec): 2537-41.

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