A4.74

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$40.00
SKU: A4.74
View product citations for antibody A4.74 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Myosin heavy chain (human fast fibers)
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Blau, H.M.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Baxter Lab for Stem Cell Biology, Stanford University
Positive Tested Species Reactivity: Bovine, C. elegans, Horse, Human, Llama, Porcine, Quail, Rodent
Depositors Notes: Fusion: 1983. Recognizes all fast fibers in human. In rat, high affinity for IIA fibers, low affinity for IIX/D fibers, and doesn't stain IIB.
Antigen Molecular Weight: 220kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: MYH2
Immunogen: partially purified myosin (pyrophosphate extracted) from human adult (22 year old) skeletal muscle
Alternate Gene Names: BM3; MYH2A; MYPOP; MYHSA2; MYHas8; MyHC-2A; MyHC-IIa
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: 653
Epitope Location or Sequence:
Uniprot ID: Q9UKX2 
Immunogen Sequence:
Entrez Gene ID: 4620 
Additional Characterization:
Antibody Registry ID: AB_528383 
Additional Information: stains MyHCIIa [PMID 7687223]. This antibody may recognize more than one myosin isoform depending on the tissue, developmental stage, or species. The gene, Uniprot ID and gene ID associated with this mAb are not exclusive.
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
A4.74 was deposited to the DSHB by Blau, H.M. (DSHB Hybridoma Product A4.74)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

33 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • All References
  • Initial Publication
    IF References

    Fast muscle fibers are preferentially affected in Duchenne muscular dystrophy.
    Blau HM
    Cell 52.4 (1988 Feb 26): 503-13.

    Sympathetic innervation of human muscle spindles.
    Domellöf FP
    Journal of anatomy 226.6 (2015 Jun): 542-8.

    Gene expression and fiber type variations in repeated vastus lateralis biopsies.
    Svensson MB
    Muscle and nerve 52.5 (2015 Nov): 812-7.

    Muscular interleukin-6 differentially regulates skeletal muscle adaptation to high-fat diet in a sex-dependent manner.
    Hidalgo J
    Cytokine 74.1 (2015 Jul): 145-51.

    Ultrasonography detects early laryngeal muscle atrophy in an equine neurectomy model.
    Piercy RJ
    Muscle and nerve 53.4 (2016 Apr): 583-92.

    Structure-activity relationships in rodent diaphragm muscle fibers vs. neuromuscular junctions.
    Mantilla CB
    Respiratory physiology and neurobiology 180.1 (2012 Jan 15): 88-96.

    Specificity of different anti-myosin heavy chain antibodies in bovine muscle.
    Geay Y
    Meat science 55.1 (2000 May): 67-78.

    Three slow myosin heavy chains sequentially expressed in developing mammalian skeletal muscle.
    Blau HM
    Developmental biology 158.1 (1993 Jul): 183-99.

    Myosin heavy chain expression in mouse extraocular muscle: more complex than expected.
    Kaminski HJ
    Investigative ophthalmology and visual science 51.12 (2010 Dec): 6355-63.

    Muscles in a mouse model of spinal muscular atrophy show profound defects in neuromuscular development even in the absence of failure in neuromuscular transmission or loss of motor neurons.
    Thompson W
    Developmental biology 356.2 (2011 Aug 15): 432-44.

    IGF-1R Reduction Triggers Neuroprotective Signaling Pathways in Spinal Muscular Atrophy Mice.
    Charbonnier F
    The Journal of neuroscience : the official journal of the Society for Neuroscience 35.34 (2015 Aug 26): 12063-79.

    Significant differences among skeletal muscles in the incorporation of bone marrow-derived cells.
    Blau HM
    Developmental biology 262.1 (2003 Oct 1): 64-74.

    WB References

    Three slow myosin heavy chains sequentially expressed in developing mammalian skeletal muscle.
    Blau HM
    Developmental biology 158.1 (1993 Jul): 183-99.

    Fast muscle fibers are preferentially affected in Duchenne muscular dystrophy.
    Blau HM
    Cell 52.4 (1988 Feb 26): 503-13.

    Enhanced technique to measure proteins in single segments of human skeletal muscle fibers: fiber-type dependence of AMPK-alpha1 and -beta1.
    Murphy RM
    Journal of applied physiology (Bethesda, Md. : 1985) 110.3 (2011 Mar): 820-5.

    Human extraocular muscles: unique pattern of myosin heavy chain expression during myotube formation.
    Thornell LE
    Investigative ophthalmology and visual science 41.7 (2000 Jun): 1608-16.

    Myosin heavy chain composition of muscle spindles in human biceps brachii.
    Pedrosa-Domellöf F
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 50.2 (2002 Feb): 171-83.

    Mild heat stress enhances differentiation and proliferation of Japanese quail myoblasts and enhances slow muscle fiber characteristics.
    Lee K
    Poultry science 95.8 (2016 Aug 1): 1912-7.

    Specificity of different anti-myosin heavy chain antibodies in bovine muscle.
    Geay Y
    Meat science 55.1 (2000 May): 67-78.

    Postnatal development of myosin heavy chain isoforms in rat laryngeal muscles.
    Flint PW
    The Annals of otology, rhinology, and laryngology 108.5 (1999 May): 509-15.

    Chronic congestive heart failure elicits adaptations of endurance exercise in diaphragmatic muscle.
    Mancini D
    Circulation 95.4 (1997 Feb 18): 910-6.

    Myosin heavy chain composition in human laryngeal muscles.
    Flint PW
    The Laryngoscope 109.9 (1999 Sep): 1521-4.

    Changes in myofibrillar protein composition of human diaphragm elicited by congestive heart failure.
    Levine S
    Journal of molecular and cellular cardiology 28.12 (1996 Dec): 2537-41.

    IHC References

    Differential response of skeletal muscles to mTORC1 signaling during atrophy and hypertrophy.
    Rüegg MA
    Skeletal muscle 3.1 (2013 Mar 6): 6.

    A hypoplastic model of skeletal muscle development displaying reduced foetal myoblast cell numbers, increased oxidative myofibres and improved specific tension capacity.
    Patel K
    Developmental biology 343.1-2 (2010 Jul 1): 51-62.

    Neuromuscular partitioning, architectural design, and myosin fiber types of the M. vastus lateralis of the llama (Lama glama).
    Rivero JL
    Journal of morphology 262.2 (2004 Nov): 667-81.

    Human extraocular muscles: unique pattern of myosin heavy chain expression during myotube formation.
    Thornell LE
    Investigative ophthalmology and visual science 41.7 (2000 Jun): 1608-16.

    Human reserve pluripotent mesenchymal stem cells are present in the connective tissues of skeletal muscle and dermis derived from fetal, adult, and geriatric donors.
    Black AC Jr
    The Anatomical record 264.1 (2001 Sep 1): 51-62.

    Changes in a rat facial muscle after facial nerve injury and repair.
    Hildebrand C
    Muscle and nerve 24.9 (2001 Sep): 1202-12.

    Myosin heavy chain composition of muscle spindles in human biceps brachii.
    Pedrosa-Domellöf F
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 50.2 (2002 Feb): 171-83.

    Muscle spindles in the deep muscles of the human neck: a morphological and immunocytochemical study.
    Pedrosa-Domellöf F
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 51.2 (2003 Feb): 175-86.

    Existence of reserve quiescent stem cells in adults, from amphibians to humans.
    Young HE
    Current topics in microbiology and immunology 280. (2004): 71-109.

    Adult stem cells.
    Black AC Jr
    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 276.1 (2004 Jan): 75-102.

    Adaptive functional specialisation of architectural design and fibre type characteristics in agonist shoulder flexor muscles of the llama, Lama glama.
    Rivero JL
    Journal of anatomy 221.2 (2012 Aug): 151-63.

    Muscle fiber pattern is independent of cell lineage in postnatal rodent development.
    Blau HM
    Cell 68.4 (1992 Feb 21): 659-71.

    Muscle spindles in the human bulbospongiosus and ischiocavernosus muscles.
    May CA
    Muscle and nerve 52.1 (2015 Jul): 55-62.

    Evidence for differential post-translational modifications of slow myosin heavy chain during murine skeletal muscle development.
    Hughes SM
    Journal of muscle research and cell motility 21.2 (2000 Feb): 101-13.

    Intrafusal myosin heavy chain expression of human masseter and biceps muscles at young age shows fundamental similarities but also marked differences.
    Eriksson PO
    Histochemistry and cell biology 139.6 (2013 Jun): 895-907.

    ELISA References
    All References

    Differential response of skeletal muscles to mTORC1 signaling during atrophy and hypertrophy.
    Rüegg MA
    Skeletal muscle 3.1 (2013 Mar 6): 6.

    A hypoplastic model of skeletal muscle development displaying reduced foetal myoblast cell numbers, increased oxidative myofibres and improved specific tension capacity.
    Patel K
    Developmental biology 343.1-2 (2010 Jul 1): 51-62.

    Neuromuscular partitioning, architectural design, and myosin fiber types of the M. vastus lateralis of the llama (Lama glama).
    Rivero JL
    Journal of morphology 262.2 (2004 Nov): 667-81.

    Human extraocular muscles: unique pattern of myosin heavy chain expression during myotube formation.
    Thornell LE
    Investigative ophthalmology and visual science 41.7 (2000 Jun): 1608-16.

    Human reserve pluripotent mesenchymal stem cells are present in the connective tissues of skeletal muscle and dermis derived from fetal, adult, and geriatric donors.
    Black AC Jr
    The Anatomical record 264.1 (2001 Sep 1): 51-62.

    Changes in a rat facial muscle after facial nerve injury and repair.
    Hildebrand C
    Muscle and nerve 24.9 (2001 Sep): 1202-12.

    Myosin heavy chain composition of muscle spindles in human biceps brachii.
    Pedrosa-Domellöf F
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 50.2 (2002 Feb): 171-83.

    Muscle spindles in the deep muscles of the human neck: a morphological and immunocytochemical study.
    Pedrosa-Domellöf F
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 51.2 (2003 Feb): 175-86.

    Existence of reserve quiescent stem cells in adults, from amphibians to humans.
    Young HE
    Current topics in microbiology and immunology 280. (2004): 71-109.

    Adult stem cells.
    Black AC Jr
    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 276.1 (2004 Jan): 75-102.

    Adaptive functional specialisation of architectural design and fibre type characteristics in agonist shoulder flexor muscles of the llama, Lama glama.
    Rivero JL
    Journal of anatomy 221.2 (2012 Aug): 151-63.

    Muscle fiber pattern is independent of cell lineage in postnatal rodent development.
    Blau HM
    Cell 68.4 (1992 Feb 21): 659-71.

    Muscle spindles in the human bulbospongiosus and ischiocavernosus muscles.
    May CA
    Muscle and nerve 52.1 (2015 Jul): 55-62.

    Evidence for differential post-translational modifications of slow myosin heavy chain during murine skeletal muscle development.
    Hughes SM
    Journal of muscle research and cell motility 21.2 (2000 Feb): 101-13.

    Intrafusal myosin heavy chain expression of human masseter and biceps muscles at young age shows fundamental similarities but also marked differences.
    Eriksson PO
    Histochemistry and cell biology 139.6 (2013 Jun): 895-907.

    Fast muscle fibers are preferentially affected in Duchenne muscular dystrophy.
    Blau HM
    Cell 52.4 (1988 Feb 26): 503-13.

    Sympathetic innervation of human muscle spindles.
    Domellöf FP
    Journal of anatomy 226.6 (2015 Jun): 542-8.

    Gene expression and fiber type variations in repeated vastus lateralis biopsies.
    Svensson MB
    Muscle and nerve 52.5 (2015 Nov): 812-7.

    Muscular interleukin-6 differentially regulates skeletal muscle adaptation to high-fat diet in a sex-dependent manner.
    Hidalgo J
    Cytokine 74.1 (2015 Jul): 145-51.

    Ultrasonography detects early laryngeal muscle atrophy in an equine neurectomy model.
    Piercy RJ
    Muscle and nerve 53.4 (2016 Apr): 583-92.

    Structure-activity relationships in rodent diaphragm muscle fibers vs. neuromuscular junctions.
    Mantilla CB
    Respiratory physiology and neurobiology 180.1 (2012 Jan 15): 88-96.

    Specificity of different anti-myosin heavy chain antibodies in bovine muscle.
    Geay Y
    Meat science 55.1 (2000 May): 67-78.

    Three slow myosin heavy chains sequentially expressed in developing mammalian skeletal muscle.
    Blau HM
    Developmental biology 158.1 (1993 Jul): 183-99.

    Myosin heavy chain expression in mouse extraocular muscle: more complex than expected.
    Kaminski HJ
    Investigative ophthalmology and visual science 51.12 (2010 Dec): 6355-63.

    Muscles in a mouse model of spinal muscular atrophy show profound defects in neuromuscular development even in the absence of failure in neuromuscular transmission or loss of motor neurons.
    Thompson W
    Developmental biology 356.2 (2011 Aug 15): 432-44.

    IGF-1R Reduction Triggers Neuroprotective Signaling Pathways in Spinal Muscular Atrophy Mice.
    Charbonnier F
    The Journal of neuroscience : the official journal of the Society for Neuroscience 35.34 (2015 Aug 26): 12063-79.

    Significant differences among skeletal muscles in the incorporation of bone marrow-derived cells.
    Blau HM
    Developmental biology 262.1 (2003 Oct 1): 64-74.

    Enhanced technique to measure proteins in single segments of human skeletal muscle fibers: fiber-type dependence of AMPK-alpha1 and -beta1.
    Murphy RM
    Journal of applied physiology (Bethesda, Md. : 1985) 110.3 (2011 Mar): 820-5.

    Mild heat stress enhances differentiation and proliferation of Japanese quail myoblasts and enhances slow muscle fiber characteristics.
    Lee K
    Poultry science 95.8 (2016 Aug 1): 1912-7.

    Postnatal development of myosin heavy chain isoforms in rat laryngeal muscles.
    Flint PW
    The Annals of otology, rhinology, and laryngology 108.5 (1999 May): 509-15.

    Chronic congestive heart failure elicits adaptations of endurance exercise in diaphragmatic muscle.
    Mancini D
    Circulation 95.4 (1997 Feb 18): 910-6.

    Myosin heavy chain composition in human laryngeal muscles.
    Flint PW
    The Laryngoscope 109.9 (1999 Sep): 1521-4.

    Changes in myofibrillar protein composition of human diaphragm elicited by congestive heart failure.
    Levine S
    Journal of molecular and cellular cardiology 28.12 (1996 Dec): 2537-41.

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