A4.951
Catalog Fields
Antigen: Myosin heavy chain (human slow fibers)
Available to For-Profits: Yes
Antigen Species: Human
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1, kappa light chain
Depositor: Blau, H.M.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Fish, Human, Quail, Rodent
Depositors Institution: Stanford University, Baxter Lab for Stem Cell Biology
Antigen Molecular Weight: 220kDa
Depositors Notes: Fusion: 1983. Specific for slow fibers.
Immunogen: partially purified myosin (pyrophosphate extracted) from human adult (22 year old) skeletal muscle
Gene: MYH7
Alternate Antibody Name:
Alternate Gene Names: CMH1; MPD1; SPMD; SPMM; CMD1S; MYHCB
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: 653
Epitope Mapped: Yes
Uniprot ID: P12883
Epitope Location or Sequence: N-terminal first 100 aa
Entrez Gene ID: 4625
Immunogen Sequence:
Antibody Registry ID: AB_528385
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Additional Information: Stains second developmental MyHCI and the epitope on slow MHC is different from that of mAbs A4.840 and N2.261 [PMID 7687223]
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
A4.951 was deposited to the DSHB by Blau, H.M. (DSHB Hybridoma Product A4.951)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
23 References
Initial Publication
Fast muscle fibers are preferentially affected in Duchenne muscular dystrophy.
Blau HM
Cell 52.4 (1988 Feb 26): 503-13.