A4.951
Catalog Fields
Antigen: Myosin heavy chain (human slow fibers)
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Blau, H.M.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Baxter Lab for Stem Cell Biology, Stanford University
Positive Tested Species Reactivity: Fish, Human, Quail, Rodent
Depositors Notes: Fusion: 1983. Specific for slow fibers.
Antigen Molecular Weight: 220kDa
Human Protein Atlas:
Predicted Species Reactivity:
Gene: MYH7
Immunogen: partially purified myosin (pyrophosphate extracted) from human adult (22 year old) skeletal muscle
Alternate Gene Names: CMH1; MPD1; SPMD; SPMM; CMD1S; MYHCB
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: 653
Epitope Location or Sequence: N-terminal first 100 aa
Uniprot ID: P12883
Immunogen Sequence:
Entrez Gene ID: 4625
Additional Characterization:
Antibody Registry ID: AB_528385
Additional Information: Stains second developmental MyHCI and the epitope on slow MHC is different from that of mAbs A4.840 and N2.261 [PMID 7687223]
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
A4.951 was deposited to the DSHB by Blau, H.M. (DSHB Hybridoma Product A4.951)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
22 References
Initial Publication
Fast muscle fibers are preferentially affected in Duchenne muscular dystrophy.
Blau HM
Cell 52.4 (1988 Feb 26): 503-13.