A4.951

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$40.00
SKU: A4.951
View product citations for antibody A4.951 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Myosin heavy chain (human slow fibers)
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Blau, H.M.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Baxter Lab for Stem Cell Biology, Stanford University
Positive Tested Species Reactivity: Fish, Human, Quail, Rodent
Depositors Notes: Fusion: 1983. Specific for slow fibers.
Antigen Molecular Weight: 220kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: MYH7
Immunogen: partially purified myosin (pyrophosphate extracted) from human adult (22 year old) skeletal muscle
Alternate Gene Names: CMH1; MPD1; SPMD; SPMM; CMD1S; MYHCB
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: 653
Epitope Location or Sequence: N-terminal first 100 aa
Uniprot ID: P12883 
Immunogen Sequence:
Entrez Gene ID: 4625 
Additional Characterization:
Antibody Registry ID: AB_528385 
Additional Information: Stains second developmental MyHCI and the epitope on slow MHC is different from that of mAbs A4.840 and N2.261 [PMID 7687223]
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
A4.951 was deposited to the DSHB by Blau, H.M. (DSHB Hybridoma Product A4.951)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

22 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Fast muscle fibers are preferentially affected in Duchenne muscular dystrophy.
    Blau HM
    Cell 52.4 (1988 Feb 26): 503-13.

    Fiber type-specific response of skeletal muscle satellite cells to high-intensity resistance training in dialysis patients.
    Mackey AL
    Muscle and nerve 52.5 (2015 Nov): 736-45.

    Long-term quercetin dietary enrichment decreases muscle injury in mdx mice.
    Selsby JT
    Clinical nutrition (Edinburgh, Scotland) 34.3 (2015 Jun): 515-22.

    Activation of satellite cells and the regeneration of human skeletal muscle are expedited by ingestion of nonsteroidal anti-inflammatory medication.
    Kjaer M
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 30.6 (2016 Jun): 2266-81.

    Satellite cell activity, without expansion, after nonhypertrophic stimuli.
    Parise G
    American journal of physiology. Regulatory, integrative and comparative physiology 309.9 (2015 Nov 1): R1101-11.

    Evidence for differential post-translational modifications of slow myosin heavy chain during murine skeletal muscle development.
    Hughes SM
    Journal of muscle research and cell motility 21.2 (2000 Feb): 101-13.

    Three slow myosin heavy chains sequentially expressed in developing mammalian skeletal muscle.
    Blau HM
    Developmental biology 158.1 (1993 Jul): 183-99.

    Regulation of myosin heavy chain expression in adult rat hindlimb muscles during short-term paralysis: comparison of denervation and tetrodotoxin-induced neural inactivation.
    Dunn SE
    FEBS letters 391.1-2 (1996 Aug 5): 39-44.

    The influence of capillarization on satellite cell pool expansion and activation following exercise-induced muscle damage in healthy young men.
    Parise G
    The Journal of physiology 596.6 (2018 Mar 15): 1063-1078.

    WB References

    Three slow myosin heavy chains sequentially expressed in developing mammalian skeletal muscle.
    Blau HM
    Developmental biology 158.1 (1993 Jul): 183-99.

    Fast muscle fibers are preferentially affected in Duchenne muscular dystrophy.
    Blau HM
    Cell 52.4 (1988 Feb 26): 503-13.

    Evidence for myoblast-extrinsic regulation of slow myosin heavy chain expression during muscle fiber formation in embryonic development.
    Blau HM
    The Journal of cell biology 121.4 (1993 May): 795-810.

    Human extraocular muscles: unique pattern of myosin heavy chain expression during myotube formation.
    Thornell LE
    Investigative ophthalmology and visual science 41.7 (2000 Jun): 1608-16.

    Myosin heavy chain composition of muscle spindles in human biceps brachii.
    Pedrosa-Domellöf F
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 50.2 (2002 Feb): 171-83.

    Mild heat stress enhances differentiation and proliferation of Japanese quail myoblasts and enhances slow muscle fiber characteristics.
    Lee K
    Poultry science 95.8 (2016 Aug 1): 1912-7.

    Chronic congestive heart failure elicits adaptations of endurance exercise in diaphragmatic muscle.
    Mancini D
    Circulation 95.4 (1997 Feb 18): 910-6.

    Myosin heavy chain composition in human laryngeal muscles.
    Flint PW
    The Laryngoscope 109.9 (1999 Sep): 1521-4.

    Changes in myofibrillar protein composition of human diaphragm elicited by congestive heart failure.
    Levine S
    Journal of molecular and cellular cardiology 28.12 (1996 Dec): 2537-41.

    Cellular adaptations in the diaphragm in chronic obstructive pulmonary disease.
    Tikunov B
    The New England journal of medicine 337.25 (1997 Dec 18): 1799-806.

    IHC References

    Evidence for myoblast-extrinsic regulation of slow myosin heavy chain expression during muscle fiber formation in embryonic development.
    Blau HM
    The Journal of cell biology 121.4 (1993 May): 795-810.

    Human extraocular muscles: unique pattern of myosin heavy chain expression during myotube formation.
    Thornell LE
    Investigative ophthalmology and visual science 41.7 (2000 Jun): 1608-16.

    Myosin heavy chain composition of muscle spindles in human biceps brachii.
    Pedrosa-Domellöf F
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 50.2 (2002 Feb): 171-83.

    Muscle spindles in the deep muscles of the human neck: a morphological and immunocytochemical study.
    Pedrosa-Domellöf F
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 51.2 (2003 Feb): 175-86.

    Subcellular immunolocalisation of fatty acid translocase (FAT)/CD36 in human type-1 and type-2 skeletal muscle fibres.
    Luiken JJ
    Histochemistry and cell biology 121.2 (2004 Feb): 101-7.

    Evidence for differential post-translational modifications of slow myosin heavy chain during murine skeletal muscle development.
    Hughes SM
    Journal of muscle research and cell motility 21.2 (2000 Feb): 101-13.

    Coordinated expression of myosin heavy chain isoforms and metabolic enzymes within overloaded rat muscle fibers.
    Michel RN
    The American journal of physiology 273.2 Pt 1 (1997 Aug): C371-83.

    Intrafusal myosin heavy chain expression of human masseter and biceps muscles at young age shows fundamental similarities but also marked differences.
    Eriksson PO
    Histochemistry and cell biology 139.6 (2013 Jun): 895-907.

    Different Training-Induced Skeletal Muscle Adaptations in COPD Patients with and without Alpha-1 Antitrypsin Deficiency.
    Kenn K
    Respiration; international review of thoracic diseases 92.5 (2016): 339-347.

    ELISA References
    Epitope Map References
    All References

    Evidence for myoblast-extrinsic regulation of slow myosin heavy chain expression during muscle fiber formation in embryonic development.
    Blau HM
    The Journal of cell biology 121.4 (1993 May): 795-810.

    Human extraocular muscles: unique pattern of myosin heavy chain expression during myotube formation.
    Thornell LE
    Investigative ophthalmology and visual science 41.7 (2000 Jun): 1608-16.

    Myosin heavy chain composition of muscle spindles in human biceps brachii.
    Pedrosa-Domellöf F
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 50.2 (2002 Feb): 171-83.

    Muscle spindles in the deep muscles of the human neck: a morphological and immunocytochemical study.
    Pedrosa-Domellöf F
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 51.2 (2003 Feb): 175-86.

    Subcellular immunolocalisation of fatty acid translocase (FAT)/CD36 in human type-1 and type-2 skeletal muscle fibres.
    Luiken JJ
    Histochemistry and cell biology 121.2 (2004 Feb): 101-7.

    Evidence for differential post-translational modifications of slow myosin heavy chain during murine skeletal muscle development.
    Hughes SM
    Journal of muscle research and cell motility 21.2 (2000 Feb): 101-13.

    Coordinated expression of myosin heavy chain isoforms and metabolic enzymes within overloaded rat muscle fibers.
    Michel RN
    The American journal of physiology 273.2 Pt 1 (1997 Aug): C371-83.

    Intrafusal myosin heavy chain expression of human masseter and biceps muscles at young age shows fundamental similarities but also marked differences.
    Eriksson PO
    Histochemistry and cell biology 139.6 (2013 Jun): 895-907.

    Different Training-Induced Skeletal Muscle Adaptations in COPD Patients with and without Alpha-1 Antitrypsin Deficiency.
    Kenn K
    Respiration; international review of thoracic diseases 92.5 (2016): 339-347.

    Fast muscle fibers are preferentially affected in Duchenne muscular dystrophy.
    Blau HM
    Cell 52.4 (1988 Feb 26): 503-13.

    Fiber type-specific response of skeletal muscle satellite cells to high-intensity resistance training in dialysis patients.
    Mackey AL
    Muscle and nerve 52.5 (2015 Nov): 736-45.

    Long-term quercetin dietary enrichment decreases muscle injury in mdx mice.
    Selsby JT
    Clinical nutrition (Edinburgh, Scotland) 34.3 (2015 Jun): 515-22.

    Activation of satellite cells and the regeneration of human skeletal muscle are expedited by ingestion of nonsteroidal anti-inflammatory medication.
    Kjaer M
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 30.6 (2016 Jun): 2266-81.

    Satellite cell activity, without expansion, after nonhypertrophic stimuli.
    Parise G
    American journal of physiology. Regulatory, integrative and comparative physiology 309.9 (2015 Nov 1): R1101-11.

    Three slow myosin heavy chains sequentially expressed in developing mammalian skeletal muscle.
    Blau HM
    Developmental biology 158.1 (1993 Jul): 183-99.

    Regulation of myosin heavy chain expression in adult rat hindlimb muscles during short-term paralysis: comparison of denervation and tetrodotoxin-induced neural inactivation.
    Dunn SE
    FEBS letters 391.1-2 (1996 Aug 5): 39-44.

    The influence of capillarization on satellite cell pool expansion and activation following exercise-induced muscle damage in healthy young men.
    Parise G
    The Journal of physiology 596.6 (2018 Mar 15): 1063-1078.

    Mild heat stress enhances differentiation and proliferation of Japanese quail myoblasts and enhances slow muscle fiber characteristics.
    Lee K
    Poultry science 95.8 (2016 Aug 1): 1912-7.

    Chronic congestive heart failure elicits adaptations of endurance exercise in diaphragmatic muscle.
    Mancini D
    Circulation 95.4 (1997 Feb 18): 910-6.

    Myosin heavy chain composition in human laryngeal muscles.
    Flint PW
    The Laryngoscope 109.9 (1999 Sep): 1521-4.

    Changes in myofibrillar protein composition of human diaphragm elicited by congestive heart failure.
    Levine S
    Journal of molecular and cellular cardiology 28.12 (1996 Dec): 2537-41.

    Cellular adaptations in the diaphragm in chronic obstructive pulmonary disease.
    Tikunov B
    The New England journal of medicine 337.25 (1997 Dec 18): 1799-806.

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