N2.261

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$40.00
SKU: N2.261
View product citations for antibody N2.261 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Myosin heavy chain (human all fibers except fast IIb)
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Blau, H.M.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Stanford University School of Medicine
Positive Tested Species Reactivity: Human, Rodent, Zebrafish
Depositors Notes: Fusion: 1983.
Antigen Molecular Weight: 220kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: MYH7
Immunogen: partially purified myosin (pyrophosphate extracted) from human neonatal (5 days old) skeletal muscle
Alternate Gene Names: CMH1; MPD1; SPMD; SPMM; CMD1S; MYHCB
Alternate Antibody Name: Anti-MyHCI/3rd+IIa
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/2
Epitope Location or Sequence: N-terminal first 100 aa
Uniprot ID: P12883 
Immunogen Sequence: Full length protein
Entrez Gene ID: 4625 
Additional Characterization:
Antibody Registry ID: AB_531790 
Additional Information: Stains MyHCIIa and third developmental stage MyHCI, and the epitope on slow MHC is different from that of mAbs A4.951 and A4.951 [PMID 7687223]. Stains all fibers except fast IIb [PMID 3342447; PMID 11005719]. This antibody may recognize more than one myosin isoform depending on the tissue, developmental stage, or species. The gene, Uniprot ID and gene ID associated with this mAb are not exclusive. RRID: AB_531790
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
N2.261 was deposited to the DSHB by Blau, H.M. (DSHB Hybridoma Product N2.261)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

18 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Fast muscle fibers are preferentially affected in Duchenne muscular dystrophy.
    Blau HM
    Cell 52.4 (1988 Feb 26): 503-13.

    Three slow myosin heavy chains sequentially expressed in developing mammalian skeletal muscle.
    Blau HM
    Developmental biology 158.1 (1993 Jul): 183-99.

    A modified PAS stain combined with immunofluorescence for quantitative analyses of glycogen in muscle sections.
    Hesselink MK
    Histochemistry and cell biology 122.2 (2004 Aug): 161-9.

    A dual epimorphic and compensatory mode of heart regeneration in zebrafish.
    Jaźwińska A
    Developmental biology 399.1 (2015 Mar 1): 27-40.

    Rac1-PAK2 pathway is essential for zebrafish heart regeneration.
    Zhong TP
    Biochemical and biophysical research communications 472.4 (2016 Apr 15): 637-42.

    Targeting of Fn14 Prevents Cancer-Induced Cachexia and Prolongs Survival.
    Hoogenraad NJ
    Cell 162.6 (2015 Sep 10): 1365-78.

    Gene expression and fiber type variations in repeated vastus lateralis biopsies.
    Svensson MB
    Muscle and nerve 52.5 (2015 Nov): 812-7.

    WB References

    Three slow myosin heavy chains sequentially expressed in developing mammalian skeletal muscle.
    Blau HM
    Developmental biology 158.1 (1993 Jul): 183-99.

    Characterisation of human soft palate muscles with respect to fibre types, myosins and capillary supply.
    Lindman R
    Journal of anatomy 197 ( Pt 2). (2000 Aug): 275-90.

    Morphological characterization of the levator veli palatini muscle in children born with cleft palates.
    Stål PS
    The Cleft palate-craniofacial journal : official publication of the American Cleft Palate-Craniofacial Association 38.5 (2001 Sep): 438-48.

    Abnormal palatopharyngeal muscle morphology in sleep-disordered breathing.
    Stål PS
    Journal of the neurological sciences 195.1 (2002 Mar 15): 11-23.

    Myosin heavy chain composition of muscle spindles in human biceps brachii.
    Pedrosa-Domellöf F
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 50.2 (2002 Feb): 171-83.

    The actin regulator N-WASp is required for muscle-cell fusion in mice.
    Schejter ED
    Proceedings of the National Academy of Sciences of the United States of America 109.28 (2012 Jul 10): 11211-6.

    Absence of morphological and molecular correlates of sarcopenia in the macaque tongue muscle styloglossus.
    Luo Q
    Experimental gerontology 84. (2016 Nov): 40-48.

    Absence of developmental and unconventional myosin heavy chain in human suprahyoid muscles.
    Sokoloff AJ
    Muscle and nerve 49.4 (2014 Apr): 534-44.

    IHC References

    Three slow myosin heavy chains sequentially expressed in developing mammalian skeletal muscle.
    Blau HM
    Developmental biology 158.1 (1993 Jul): 183-99.

    Human extraocular muscles: unique pattern of myosin heavy chain expression during myotube formation.
    Thornell LE
    Investigative ophthalmology and visual science 41.7 (2000 Jun): 1608-16.

    Characterisation of human soft palate muscles with respect to fibre types, myosins and capillary supply.
    Lindman R
    Journal of anatomy 197 ( Pt 2). (2000 Aug): 275-90.

    Morphological characterization of the levator veli palatini muscle in children born with cleft palates.
    Stål PS
    The Cleft palate-craniofacial journal : official publication of the American Cleft Palate-Craniofacial Association 38.5 (2001 Sep): 438-48.

    Changes in a rat facial muscle after facial nerve injury and repair.
    Hildebrand C
    Muscle and nerve 24.9 (2001 Sep): 1202-12.

    Abnormal palatopharyngeal muscle morphology in sleep-disordered breathing.
    Stål PS
    Journal of the neurological sciences 195.1 (2002 Mar 15): 11-23.

    Myosin heavy chain composition of muscle spindles in human biceps brachii.
    Pedrosa-Domellöf F
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 50.2 (2002 Feb): 171-83.

    Muscle spindles in the deep muscles of the human neck: a morphological and immunocytochemical study.
    Pedrosa-Domellöf F
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 51.2 (2003 Feb): 175-86.

    Resveratrol modulates the angiogenic response to exercise training in skeletal muscles of aged men.
    Hellsten Y
    American journal of physiology. Heart and circulatory physiology 307.8 (2014 Oct 15): H1111-9.

    ELISA References
    Epitope Map References
    All References

    Three slow myosin heavy chains sequentially expressed in developing mammalian skeletal muscle.
    Blau HM
    Developmental biology 158.1 (1993 Jul): 183-99.

    Human extraocular muscles: unique pattern of myosin heavy chain expression during myotube formation.
    Thornell LE
    Investigative ophthalmology and visual science 41.7 (2000 Jun): 1608-16.

    Characterisation of human soft palate muscles with respect to fibre types, myosins and capillary supply.
    Lindman R
    Journal of anatomy 197 ( Pt 2). (2000 Aug): 275-90.

    Morphological characterization of the levator veli palatini muscle in children born with cleft palates.
    Stål PS
    The Cleft palate-craniofacial journal : official publication of the American Cleft Palate-Craniofacial Association 38.5 (2001 Sep): 438-48.

    Changes in a rat facial muscle after facial nerve injury and repair.
    Hildebrand C
    Muscle and nerve 24.9 (2001 Sep): 1202-12.

    Abnormal palatopharyngeal muscle morphology in sleep-disordered breathing.
    Stål PS
    Journal of the neurological sciences 195.1 (2002 Mar 15): 11-23.

    Myosin heavy chain composition of muscle spindles in human biceps brachii.
    Pedrosa-Domellöf F
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 50.2 (2002 Feb): 171-83.

    Muscle spindles in the deep muscles of the human neck: a morphological and immunocytochemical study.
    Pedrosa-Domellöf F
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 51.2 (2003 Feb): 175-86.

    Resveratrol modulates the angiogenic response to exercise training in skeletal muscles of aged men.
    Hellsten Y
    American journal of physiology. Heart and circulatory physiology 307.8 (2014 Oct 15): H1111-9.

    Fast muscle fibers are preferentially affected in Duchenne muscular dystrophy.
    Blau HM
    Cell 52.4 (1988 Feb 26): 503-13.

    A modified PAS stain combined with immunofluorescence for quantitative analyses of glycogen in muscle sections.
    Hesselink MK
    Histochemistry and cell biology 122.2 (2004 Aug): 161-9.

    A dual epimorphic and compensatory mode of heart regeneration in zebrafish.
    Jaźwińska A
    Developmental biology 399.1 (2015 Mar 1): 27-40.

    Rac1-PAK2 pathway is essential for zebrafish heart regeneration.
    Zhong TP
    Biochemical and biophysical research communications 472.4 (2016 Apr 15): 637-42.

    Targeting of Fn14 Prevents Cancer-Induced Cachexia and Prolongs Survival.
    Hoogenraad NJ
    Cell 162.6 (2015 Sep 10): 1365-78.

    Gene expression and fiber type variations in repeated vastus lateralis biopsies.
    Svensson MB
    Muscle and nerve 52.5 (2015 Nov): 812-7.

    The actin regulator N-WASp is required for muscle-cell fusion in mice.
    Schejter ED
    Proceedings of the National Academy of Sciences of the United States of America 109.28 (2012 Jul 10): 11211-6.

    Absence of morphological and molecular correlates of sarcopenia in the macaque tongue muscle styloglossus.
    Luo Q
    Experimental gerontology 84. (2016 Nov): 40-48.

    Absence of developmental and unconventional myosin heavy chain in human suprahyoid muscles.
    Sokoloff AJ
    Muscle and nerve 49.4 (2014 Apr): 534-44.

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