(0) No Reviews yet
$45.00 to $425.00
View product citations for antibody ADL40 on CiteAb

In Stock

Available: 38

DSHB Data Sheet

Catalog Fields

Antigen: lamin Dm0
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Fisher, P. A.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: SUNY at Stony Brook, Pharmacological Sciences
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Does not react with Sf9 lamin. Reacts with all Dm isoforms; does not react with lamin C. Epitope between aa 336-410 (coil 2).
Antigen Molecular Weight: Predicted: 71 kDa; Apparent: 74 and 76 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Lam
Immunogen: Recombinant total protein
Alternate Gene Names: Dm0; lamin Dm0; Dm1; Dm2
Alternate Antibody Name: ADL67; alphaL528-622
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS0
Epitope Location or Sequence: aa 336-410 [Depositor Notes]
Uniprot ID: P08928 
Immunogen Sequence: Total protein
Entrez Gene ID: 33782 
Additional Characterization:  
Antibody Registry ID: AB_528334 
Additional Information:
Recommended Applications: Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
ADL40 was deposited to the DSHB by Fisher, P. A. (DSHB Hybridoma Product ADL40)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • All References
  • Initial Publication
    All References

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review