C1A9

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$40.00
SKU: C1A9
View product citations for antibody C1A9 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Heterochromatin Protein 1
Hybridoma Cells Available: Yes
Antigen Species: Drosophila melanogaster
Depositor: Wallrath, L.L.
Isotype: MIgG1, kappa light chain
Depositors Institution: University of Iowa
Host Species: mouse
Depositors Notes: detects HP1, a structural component of heterochromatin, on fixed squashed and whole mount Drosophila melanogaster salivary gland polytene chromosomes
Positive Tested Species Reactivity: Drosophila, Planococcus citri
Gene: Su(var)205
Antigen Molecular Weight: Predicted: 23 kDa; Apparent 24 kDa
Alternate Gene Names: HP1, CG8409
Immunogen: Nuclear proteins extracted in 2.0 M potassium thiocyanate
Clonality: Monoclonal
Myeloma Strain: Sp2/0 Ag14
Epitope Mapped: No
Uniprot ID: P05205 
Immunogen Sequence: Total protein
Entrez Gene ID: 34119 
Antibody Registry ID: AB_528276 
Recommended Applications: Chromatin Immunoprecipitation, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
C1A9 was deposited to the DSHB by Wallrath, L.L. (DSHB Hybridoma Product C1A9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

22 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • All References
  • Initial Publication
    IF References

    Identification of a nonhistone chromosomal protein associated with heterochromatin in Drosophila melanogaster and its gene.
    Elgin SC
    Molecular and cellular biology 6.11 (1986 Nov): 3862-72.

    SU(VAR)3-7, a Drosophila heterochromatin-associated protein and companion of HP1 in the genomic silencing of position-effect variegation.
    Spierer P
    The EMBO journal 16.17 (1997 Sep 1): 5280-8.

    The heterochromatin protein 1 prevents telomere fusions in Drosophila.
    Pimpinelli S
    Molecular cell 2.5 (1998 Nov): 527-38.

    Chromosomal distribution of heterochromatin protein 1 (HP1) in Drosophila: a cytological map of euchromatic HP1 binding sites.
    Pimpinelli S
    Genetica 117.2-3 (2003 Mar): 135-47.

    Sex-specific role of Drosophila melanogaster HP1 in regulating chromatin structure and gene transcription.
    Sun FL
    Nature genetics 37.12 (2005 Dec): 1361-6.

    Functional analysis of the chromo domain of HP1.
    Eissenberg JC
    The EMBO journal 14.16 (1995 Aug 15): 3977-86.

    JAK signaling globally counteracts heterochromatic gene silencing.
    Li WX
    Nature genetics 38.9 (2006 Sep): 1071-6.

    Heterochromatin protein 1 (HP1) is associated with induced gene expression in Drosophila euchromatin.
    Pimpinelli S
    The Journal of cell biology 161.4 (2003 May 26): 707-14.

    HP1 controls telomere capping, telomere elongation, and telomere silencing by two different mechanisms in Drosophila.
    Pimpinelli S
    Molecular cell 15.3 (2004 Aug 13): 467-76.

    Role of Drosophila HP1 in euchromatic gene expression.
    Wallrath LL
    Developmental dynamics : an official publication of the American Association of Anatomists 232.3 (2005 Mar): 767-74.

    Epigenetic regulation of facultative heterochromatinisation in Planococcus citri via the Me(3)K9H3-HP1-Me(3)K20H4 pathway.
    Prantera G
    Journal of cell science 120.Pt 6 (2007 Mar 15): 1072-80.

    Drosophila STAT is required for directly maintaining HP1 localization and heterochromatin stability.
    Li WX
    Nature cell biology 10.4 (2008 Apr): 489-96.

    The metazoan-specific mediator subunit 26 (Med26) is essential for viability and is found at both active genes and pericentric heterochromatin in Drosophila melanogaster.
    Marr MT 2nd
    Molecular and cellular biology 34.14 (2014 Jul): 2710-20.

    Drosophila RISC component VIG and its homolog Vig2 impact heterochromatin formation.
    Elgin SC
    PloS one 4.7 (2009 Jul 8): e6182.

    Chromatin remodeling in the aging genome of Drosophila.
    Helfand SL
    Aging cell 9.6 (2010 Dec): 971-8.

    Heterochromatin formation promotes longevity and represses ribosomal RNA synthesis.
    Li WX
    PLoS genetics 8.1 (2012 Jan): e1002473.

    Drosophila UTX is a histone H3 Lys27 demethylase that colocalizes with the elongating form of RNA polymerase II.
    Shilatifard A
    Molecular and cellular biology 28.3 (2008 Feb): 1041-6.

    <i>Drosophila small ovary</i> gene is required for transposon silencing and heterochromatin organization, and ensures germline stem cell maintenance and differentiation.
    Erdélyi M
    Development (Cambridge, England) 145.23 (2018 Dec 4): .

    WB References

    Identification of a nonhistone chromosomal protein associated with heterochromatin in Drosophila melanogaster and its gene.
    Elgin SC
    Molecular and cellular biology 6.11 (1986 Nov): 3862-72.

    Antagonistic roles of Drosophila Tctp and Brahma in chromatin remodelling and stabilizing repeated sequences.
    Choi KW
    Nature communications 7. (2016 Sep 30): 12988.

    SU(VAR)3-7, a Drosophila heterochromatin-associated protein and companion of HP1 in the genomic silencing of position-effect variegation.
    Spierer P
    The EMBO journal 16.17 (1997 Sep 1): 5280-8.

    The Drosophila HOAP protein is required for telomere capping.
    Gatti M
    Nature cell biology 5.1 (2003 Jan): 82-4.

    Sex-specific role of Drosophila melanogaster HP1 in regulating chromatin structure and gene transcription.
    Sun FL
    Nature genetics 37.12 (2005 Dec): 1361-6.

    JAK signaling globally counteracts heterochromatic gene silencing.
    Li WX
    Nature genetics 38.9 (2006 Sep): 1071-6.

    Heterochromatin protein 1 (HP1) is associated with induced gene expression in Drosophila euchromatin.
    Pimpinelli S
    The Journal of cell biology 161.4 (2003 May 26): 707-14.

    HP1 controls telomere capping, telomere elongation, and telomere silencing by two different mechanisms in Drosophila.
    Pimpinelli S
    Molecular cell 15.3 (2004 Aug 13): 467-76.

    Drosophila STAT is required for directly maintaining HP1 localization and heterochromatin stability.
    Li WX
    Nature cell biology 10.4 (2008 Apr): 489-96.

    HP1 modulates the transcription of cell-cycle regulators in Drosophila melanogaster.
    Sun FL
    Nucleic acids research 33.9 (2005): 2852-8.

    The metazoan-specific mediator subunit 26 (Med26) is essential for viability and is found at both active genes and pericentric heterochromatin in Drosophila melanogaster.
    Marr MT 2nd
    Molecular and cellular biology 34.14 (2014 Jul): 2710-20.

    Heterochromatin formation promotes longevity and represses ribosomal RNA synthesis.
    Li WX
    PLoS genetics 8.1 (2012 Jan): e1002473.

    IHC References
    IP References
    All References

    Antagonistic roles of Drosophila Tctp and Brahma in chromatin remodelling and stabilizing repeated sequences.
    Choi KW
    Nature communications 7. (2016 Sep 30): 12988.

    Identification of a nonhistone chromosomal protein associated with heterochromatin in Drosophila melanogaster and its gene.
    Elgin SC
    Molecular and cellular biology 6.11 (1986 Nov): 3862-72.

    SU(VAR)3-7, a Drosophila heterochromatin-associated protein and companion of HP1 in the genomic silencing of position-effect variegation.
    Spierer P
    The EMBO journal 16.17 (1997 Sep 1): 5280-8.

    The heterochromatin protein 1 prevents telomere fusions in Drosophila.
    Pimpinelli S
    Molecular cell 2.5 (1998 Nov): 527-38.

    Chromosomal distribution of heterochromatin protein 1 (HP1) in Drosophila: a cytological map of euchromatic HP1 binding sites.
    Pimpinelli S
    Genetica 117.2-3 (2003 Mar): 135-47.

    Sex-specific role of Drosophila melanogaster HP1 in regulating chromatin structure and gene transcription.
    Sun FL
    Nature genetics 37.12 (2005 Dec): 1361-6.

    Functional analysis of the chromo domain of HP1.
    Eissenberg JC
    The EMBO journal 14.16 (1995 Aug 15): 3977-86.

    JAK signaling globally counteracts heterochromatic gene silencing.
    Li WX
    Nature genetics 38.9 (2006 Sep): 1071-6.

    Heterochromatin protein 1 (HP1) is associated with induced gene expression in Drosophila euchromatin.
    Pimpinelli S
    The Journal of cell biology 161.4 (2003 May 26): 707-14.

    HP1 controls telomere capping, telomere elongation, and telomere silencing by two different mechanisms in Drosophila.
    Pimpinelli S
    Molecular cell 15.3 (2004 Aug 13): 467-76.

    Role of Drosophila HP1 in euchromatic gene expression.
    Wallrath LL
    Developmental dynamics : an official publication of the American Association of Anatomists 232.3 (2005 Mar): 767-74.

    Epigenetic regulation of facultative heterochromatinisation in Planococcus citri via the Me(3)K9H3-HP1-Me(3)K20H4 pathway.
    Prantera G
    Journal of cell science 120.Pt 6 (2007 Mar 15): 1072-80.

    Drosophila STAT is required for directly maintaining HP1 localization and heterochromatin stability.
    Li WX
    Nature cell biology 10.4 (2008 Apr): 489-96.

    The metazoan-specific mediator subunit 26 (Med26) is essential for viability and is found at both active genes and pericentric heterochromatin in Drosophila melanogaster.
    Marr MT 2nd
    Molecular and cellular biology 34.14 (2014 Jul): 2710-20.

    Drosophila RISC component VIG and its homolog Vig2 impact heterochromatin formation.
    Elgin SC
    PloS one 4.7 (2009 Jul 8): e6182.

    Chromatin remodeling in the aging genome of Drosophila.
    Helfand SL
    Aging cell 9.6 (2010 Dec): 971-8.

    Heterochromatin formation promotes longevity and represses ribosomal RNA synthesis.
    Li WX
    PLoS genetics 8.1 (2012 Jan): e1002473.

    Drosophila UTX is a histone H3 Lys27 demethylase that colocalizes with the elongating form of RNA polymerase II.
    Shilatifard A
    Molecular and cellular biology 28.3 (2008 Feb): 1041-6.

    <i>Drosophila small ovary</i> gene is required for transposon silencing and heterochromatin organization, and ensures germline stem cell maintenance and differentiation.
    Erdélyi M
    Development (Cambridge, England) 145.23 (2018 Dec 4): .

    The Drosophila HOAP protein is required for telomere capping.
    Gatti M
    Nature cell biology 5.1 (2003 Jan): 82-4.

    HP1 modulates the transcription of cell-cycle regulators in Drosophila melanogaster.
    Sun FL
    Nucleic acids research 33.9 (2005): 2852-8.

    Heterochromatin protein 1 (HP1a) positively regulates euchromatic gene expression through RNA transcript association and interaction with hnRNPs in Drosophila.
    Pimpinelli S
    PLoS genetics 5.10 (2009 Oct): e1000670.

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