Antigen: Heterochromatin Protein 1
Hybridoma Cells Available: Yes
Antigen Species: Drosophila melanogaster
Depositor: Wallrath, L.L.
Isotype: MIgG1, kappa light chain
Host Species: mouse
Depositors Institution: University of Iowa
Positive Tested Species Reactivity: Drosophila, Planococcus citri
Depositors Notes: detects HP1, a structural component of heterochromatin, on fixed squashed and whole mount Drosophila melanogaster salivary gland polytene chromosomes
Antigen Molecular Weight: Predicted: 23 kDa; Apparent 24 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: Nuclear proteins extracted in 2.0 M potassium thiocyanate
Alternate Gene Names: HP1, CG8409
Alternate Antibody Name:
Alternate Antigen Name:
Myeloma Strain: Sp2/0 Ag14
Epitope Location or Sequence:
Uniprot ID: P05205
Immunogen Sequence: Total protein
Entrez Gene ID: 34119
Antibody Registry ID: AB_528276
Recommended Applications: Chromatin Immunoprecipitation, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
C1A9 was deposited to the DSHB by Wallrath, L.L. (DSHB Hybridoma Product C1A9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).