C1A9
Catalog Fields
Antigen: Heterochromatin Protein 1
Available to For-Profits: Yes
Antigen Species: Drosophila melanogaster
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1, kappa light chain
Depositor: Wallrath, L.L.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Drosophila, Planococcus citri
Depositors Institution: University of Iowa
Antigen Molecular Weight: Predicted: 23 kDa; Apparent 24 kDa
Depositors Notes: detects HP1, a structural component of heterochromatin, on fixed squashed and whole mount Drosophila melanogaster salivary gland polytene chromosomes
Immunogen: Nuclear proteins extracted in 2.0 M potassium thiocyanate
Gene: Su(var)205
Alternate Antibody Name:
Alternate Gene Names: HP1, CG8409
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: Sp2/0 Ag14
Epitope Mapped: No
Uniprot ID: P05205
Epitope Location or Sequence:
Entrez Gene ID: 34119
Immunogen Sequence: Total protein
Antibody Registry ID: AB_528276
Recommended Applications: Chromatin Immunoprecipitation, Immunofluorescence, Immunoprecipitation, Western Blot
Additional Information:
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
C1A9 was deposited to the DSHB by Wallrath, L.L. (DSHB Hybridoma Product C1A9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
26 References
Initial Publication
Identification of a nonhistone chromosomal protein associated with heterochromatin in Drosophila melanogaster and its gene.
Elgin SC
Molecular and cellular biology 6.11 (1986 Nov): 3862-72.