LC28.26

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$40.00
SKU: LC28.26
View product citations for antibody LC28.26 on CiteAb

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Available: 111

DSHB Data Sheet

Catalog Fields

Antigen: Lamin C
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Fisher, P.A.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: State University of New York at Stony Brook
Positive Tested Species Reactivity: Drosophila, Mouse
Depositors Notes: Lamins line the inner nuclear membrane to provide a structure for the nuclear envelope.
Antigen Molecular Weight: 69.8 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: LamC
Immunogen: Recombinant polypeptide pG-IF (lamin C)
Alternate Gene Names: CG10119 , pG-IF
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain:
Epitope Location or Sequence:
Uniprot ID: Q03427 
Immunogen Sequence: Full length
Entrez Gene ID: 36615 
Additional Characterization:
Antibody Registry ID: AB_528339 
Additional Information: FLYBASE: FBgn0010397
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
LC28.26 was deposited to the DSHB by Fisher, P.A. (DSHB Hybridoma Product LC28.26)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

5 References

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication
    IF References

    Expression of Drosophila lamin C is developmentally regulated: analogies with vertebrate A-type lamins.
    Weber K
    Journal of cell science 108 ( Pt 10). (1995 Oct): 3189-98.

    Methylation of histone H4 lysine 20 by PR-Set7 ensures the integrity of late replicating sequence domains in Drosophila.
    MacAlpine DM
    Nucleic acids research 44.15 (2016 Sep 6): 7204-18.

    Stress relaxation in cherry fruit.
    Blahovec J
    Biorheology 33.6 (1996 Nov-Dec): 451-62.

    Spectraplakin Shot Maintains Perinuclear Microtubule Organization in Drosophila Polyploid Cells.
    Pastor-Pareja JC
    Developmental cell 49.5 (2019 Jun 3): 731-747.e7.

    WB References

    Expression of Drosophila lamin C is developmentally regulated: analogies with vertebrate A-type lamins.
    Weber K
    Journal of cell science 108 ( Pt 10). (1995 Oct): 3189-98.

    Casein kinase 1 delta regulates the pace of the mammalian circadian clock.
    Weaver DR
    Molecular and cellular biology 29.14 (2009 Jul): 3853-66.

    All References

    Expression of Drosophila lamin C is developmentally regulated: analogies with vertebrate A-type lamins.
    Weber K
    Journal of cell science 108 ( Pt 10). (1995 Oct): 3189-98.

    Methylation of histone H4 lysine 20 by PR-Set7 ensures the integrity of late replicating sequence domains in Drosophila.
    MacAlpine DM
    Nucleic acids research 44.15 (2016 Sep 6): 7204-18.

    Stress relaxation in cherry fruit.
    Blahovec J
    Biorheology 33.6 (1996 Nov-Dec): 451-62.

    Spectraplakin Shot Maintains Perinuclear Microtubule Organization in Drosophila Polyploid Cells.
    Pastor-Pareja JC
    Developmental cell 49.5 (2019 Jun 3): 731-747.e7.

    Casein kinase 1 delta regulates the pace of the mammalian circadian clock.
    Weaver DR
    Molecular and cellular biology 29.14 (2009 Jul): 3853-66.

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