LC28.26

(0) No Reviews yet
$40.00
SKU: LC28.26
View product citations for antibody LC28.26 on CiteAb

In Stock

Available: 209

DSHB Data Sheet

Catalog Fields

Antigen: Lamin C
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Fisher, P.A.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: State University of New York at Stony Brook
Positive Tested Species Reactivity: Drosophila, Mouse
Depositors Notes: Lamins line the inner nuclear membrane to provide a structure for the nuclear envelope.
Antigen Molecular Weight: 69.8 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: LamC
Immunogen: Recombinant polypeptide pG-IF (lamin C)
Alternate Gene Names: CG10119 , pG-IF
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain:
Epitope Location or Sequence:
Uniprot ID: Q03427 
Immunogen Sequence: Full length
Entrez Gene ID: 36615 
Additional Characterization:  
Antibody Registry ID: AB_528339 
Additional Information: FLYBASE: FBgn0010397
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
LC28.26 was deposited to the DSHB by Fisher, P.A. (DSHB Hybridoma Product LC28.26)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

10 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • All References
  • Initial Publication
    IF References
    WB References

    Expression of Drosophila lamin C is developmentally regulated: analogies with vertebrate A-type lamins.
    Weber K
    Journal of cell science 108 ( Pt 10). (1995 Oct): 3189-98.

    Casein kinase 1 delta regulates the pace of the mammalian circadian clock.
    Weaver DR
    Molecular and cellular biology 29.14 (2009 Jul): 3853-66.

    Toxicity of pathogenic ataxin-2 in Drosophila shows dependence on a pure CAG repeat sequence.
    Bonini NM
    Human molecular genetics . (2021 Jun 2): .

    Piwi-piRNA complexes induce stepwise changes in nuclear architecture at target loci.
    Siomi H
    The EMBO journal 40.18 (2021 Sep 15): e108345.

    IHC References
    IP References
    All References

    Ubx-Collier signaling cascade maintains blood progenitors in the posterior lobes of the Drosophila larval lymph gland.
    Mandal L
    PLoS genetics 17.8 (2021 Aug): e1009709.

    Expression of Drosophila lamin C is developmentally regulated: analogies with vertebrate A-type lamins.
    Weber K
    Journal of cell science 108 ( Pt 10). (1995 Oct): 3189-98.

    Methylation of histone H4 lysine 20 by PR-Set7 ensures the integrity of late replicating sequence domains in Drosophila.
    MacAlpine DM
    Nucleic acids research 44.15 (2016 Sep 6): 7204-18.

    Stress relaxation in cherry fruit.
    Blahovec J
    Biorheology 33.6 (1996 Nov-Dec): 451-62.

    Spectraplakin Shot Maintains Perinuclear Microtubule Organization in Drosophila Polyploid Cells.
    Pastor-Pareja JC
    Developmental cell 49.5 (2019 Jun 3): 731-747.e7.

    Hormone receptor 4 is required in muscles and distinct ovarian cell types to regulate specific steps of <i>Drosophila</i> oogenesis.
    Drummond-Barbosa D
    Development (Cambridge, England) 148.5 (2021 Mar 9): .

    Interactions between ALS-linked FUS and nucleoporins are associated with defects in the nucleocytoplasmic transport pathway.
    Bosco DA
    Nature neuroscience 24.8 (2021 Aug): 1077-1088.

    Piwi-piRNA complexes induce stepwise changes in nuclear architecture at target loci.
    Siomi H
    The EMBO journal 40.18 (2021 Sep 15): e108345.

    Casein kinase 1 delta regulates the pace of the mammalian circadian clock.
    Weaver DR
    Molecular and cellular biology 29.14 (2009 Jul): 3853-66.

    Toxicity of pathogenic ataxin-2 in Drosophila shows dependence on a pure CAG repeat sequence.
    Bonini NM
    Human molecular genetics . (2021 Jun 2): .

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review