ADL195

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$40.00
SKU: ADL195
View product citations for antibody ADL195 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: lamin Dm0
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Fisher, P. A.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: Pharmacological Sciences, SUNY at Stony Brook
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Does not react with Sf9 lamin. Reacts with all Dm isoforms; does not react with lamin C. Epitope between aa 548-620 (tail region).
Antigen Molecular Weight: Predicted: 71 kDa; Apparent: 74 and 76 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Lam
Immunogen: Recombinant total protein
Alternate Gene Names: Dm0; lamin Dm0; Dm1; Dm2
Alternate Antibody Name: ADL84; alphaL22-28
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS0
Epitope Location or Sequence: aa 548-620 [Depositor Notes] or aa 542-622 [PMID 9372441]
Uniprot ID: P08928 
Immunogen Sequence: Total protein
Entrez Gene ID: 33782 
Additional Characterization:  
Antibody Registry ID: AB_528333 
Additional Information:
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
ADL195 was deposited to the DSHB by Fisher, P. A. (DSHB Hybridoma Product ADL195)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

9 References

  • Initial Publication
  • IF References
  • WB References
  • Epitope Map References
  • All References
  • Initial Publication

    Assembly of A- and B-type lamins studied in vivo with the baculovirus system.
    Krohne G
    Journal of cell science 110 ( Pt 20). (1997 Oct): 2519-32.

    IF References

    Assembly of A- and B-type lamins studied in vivo with the baculovirus system.
    Krohne G
    Journal of cell science 110 ( Pt 20). (1997 Oct): 2519-32.

    The multiple roles of mps1 in Drosophila female meiosis.
    Hawley RS
    PLoS genetics 3.7 (2007 Jul): e113.

    Megator, an essential coiled-coil protein that localizes to the putative spindle matrix during mitosis in Drosophila.
    Johansen KM
    Molecular biology of the cell 15.11 (2004 Nov): 4854-65.

    WB References

    Assembly of A- and B-type lamins studied in vivo with the baculovirus system.
    Krohne G
    Journal of cell science 110 ( Pt 20). (1997 Oct): 2519-32.

    Role of histone deacetylases in gene regulation at nuclear lamina.
    Nurminsky DI
    PloS one 7.11 (2012): e49692.

    Prostaglandins regulate nuclear localization of Fascin and its function in nucleolar architecture.
    Tootle TL
    Molecular biology of the cell 26.10 (2015 May 15): 1901-17.

    Epitope Map References

    Assembly of A- and B-type lamins studied in vivo with the baculovirus system.
    Krohne G
    Journal of cell science 110 ( Pt 20). (1997 Oct): 2519-32.

    All References

    Assembly of A- and B-type lamins studied in vivo with the baculovirus system.
    Krohne G
    Journal of cell science 110 ( Pt 20). (1997 Oct): 2519-32.

    The multiple roles of mps1 in Drosophila female meiosis.
    Hawley RS
    PLoS genetics 3.7 (2007 Jul): e113.

    Megator, an essential coiled-coil protein that localizes to the putative spindle matrix during mitosis in Drosophila.
    Johansen KM
    Molecular biology of the cell 15.11 (2004 Nov): 4854-65.

    Role of histone deacetylases in gene regulation at nuclear lamina.
    Nurminsky DI
    PloS one 7.11 (2012): e49692.

    Prostaglandins regulate nuclear localization of Fascin and its function in nucleolar architecture.
    Tootle TL
    Molecular biology of the cell 26.10 (2015 May 15): 1901-17.

    Developmentally regulated subnuclear genome reorganization restricts neural progenitor competence in Drosophila.
    Doe CQ
    Cell 152.1-2 (2013 Jan 17): 97-108.

    The lamin B receptor of Drosophila melanogaster.
    Krohne G
    Journal of cell science 117.Pt 10 (2004 Apr 15): 2015-28.

    The Drosophila melanogaster LEM-domain protein MAN1.
    Krohne G
    European journal of cell biology 85.2 (2006 Feb): 91-105.

    Loss of the Coffin-Lowry syndrome-associated gene RSK2 alters ERK activity, synaptic function and axonal transport in Drosophila motoneurons.
    Raabe T
    Disease models & mechanisms 8.11 (2015 Nov): 1389-400.

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