BF-32

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$40.00
SKU: BF-32
View product citations for antibody BF-32 on CiteAb

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Available: 30

DSHB Data Sheet

Catalog Fields

Antigen: Myosin heavy chain (slow and 2A)
Hybridoma Cells Available: Yes
Antigen Species: Bovine
Depositor: Schiaffino, S.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Padova
Positive Tested Species Reactivity: Human, Ovine, Rodent
Depositors Notes: Fusion: 1984. Recognizes type 1 (slow) and 2A fibers in skeletal muscle.
Antigen Molecular Weight: 220kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: MYH2
Immunogen: purified myosin from bovine skeletal muscle (fetal, 3 months)
Alternate Gene Names: MyHC-2a
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS0
Epitope Location or Sequence:
Uniprot ID: Q9BE41 
Immunogen Sequence:
Entrez Gene ID: 788772 
Additional Characterization:  
Antibody Registry ID: AB_10573811 
Additional Information: This antibody may recognize more than one myosin isoform depending on the tissue, developmental stage, or species. The gene, Uniprot ID and gene ID associated with this mAb are not exclusive.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
BF-32 was deposited to the DSHB by Schiaffino, S. (DSHB Hybridoma Product BF-32)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

4 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication

    Three myosin heavy chain isoforms in type 2 skeletal muscle fibres.
    Lømo T
    Journal of muscle research and cell motility 10.3 (1989 Jun): 197-205.

    IF References
    WB References

    Three myosin heavy chain isoforms in type 2 skeletal muscle fibres.
    Lømo T
    Journal of muscle research and cell motility 10.3 (1989 Jun): 197-205.

    Neonatal myosin heavy chains are not expressed in Ni-induced rat rhabdomyosarcoma.
    Sartore S
    Differentiation; research in biological diversity 38.1 (1988 Jun): 49-59.

    IHC References

    Three myosin heavy chain isoforms in type 2 skeletal muscle fibres.
    Lømo T
    Journal of muscle research and cell motility 10.3 (1989 Jun): 197-205.

    All References

    Three myosin heavy chain isoforms in type 2 skeletal muscle fibres.
    Lømo T
    Journal of muscle research and cell motility 10.3 (1989 Jun): 197-205.

    Intrauterine growth-restricted sheep fetuses exhibit smaller hindlimb muscle fibers and lower proportions of insulin-sensitive Type I fibers near term.
    Limesand SW
    American journal of physiology. Regulatory, integrative and comparative physiology 310.11 (2016 Jun 1): R1020-9.

    Imaging mass spectrometry reveals fiber-specific distribution of acetylcarnitine and contraction-induced carnitine dynamics in rat skeletal muscles.
    Fujii NL
    Biochimica et biophysica acta 1837.10 (2014 Oct): 1699-706.

    Neonatal myosin heavy chains are not expressed in Ni-induced rat rhabdomyosarcoma.
    Sartore S
    Differentiation; research in biological diversity 38.1 (1988 Jun): 49-59.

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