BF-32

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$40.00
SKU: BF-32
View product citations for antibody BF-32 on CiteAb

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Available: 36

DSHB Data Sheet

Catalog Fields

Antigen: Myosin heavy chain (slow and 2A)
Hybridoma Cells Available: Yes
Antigen Species: Bovine
Depositor: Schiaffino, S.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Padova
Positive Tested Species Reactivity: Human, Ovine, Rodent
Depositors Notes: Fusion: 1984. Recognizes type 1 (slow) and 2A fibers in skeletal muscle.
Antigen Molecular Weight: 220kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: MYH2
Immunogen: purified myosin from bovine skeletal muscle (fetal, 3 months)
Alternate Gene Names: MyHC-2a
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: NS0
Epitope Location or Sequence:
Uniprot ID: Q9BE41 
Immunogen Sequence:
Entrez Gene ID: 788772 
Additional Characterization:
Antibody Registry ID: AB_10573811 
Additional Information: This antibody may recognize more than one myosin isoform depending on the tissue, developmental stage, or species. The gene, Uniprot ID and gene ID associated with this mAb are not exclusive.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
BF-32 was deposited to the DSHB by Schiaffino, S. (DSHB Hybridoma Product BF-32)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

4 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication

    Journal of muscle research and cell motility
    Lømo T
    Journal of muscle research and cell motility 10.3 (1989 Jun): 197-205.

    IF References

    American journal of physiology. Regulatory, integrative and comparative physiology
    Limesand SW
    American journal of physiology. Regulatory, integrative and comparative physiology 310.11 (2016 Jun 1): R1020-9.

    Biochimica et biophysica acta
    Fujii NL
    Biochimica et biophysica acta 1837.10 (2014 Oct): 1699-706.

    WB References

    Journal of muscle research and cell motility
    Lømo T
    Journal of muscle research and cell motility 10.3 (1989 Jun): 197-205.

    Differentiation; research in biological diversity
    Sartore S
    Differentiation; research in biological diversity 38.1 (1988 Jun): 49-59.

    IHC References

    Journal of muscle research and cell motility
    Lømo T
    Journal of muscle research and cell motility 10.3 (1989 Jun): 197-205.

    All References

    Journal of muscle research and cell motility
    Lømo T
    Journal of muscle research and cell motility 10.3 (1989 Jun): 197-205.

    American journal of physiology. Regulatory, integrative and comparative physiology
    Limesand SW
    American journal of physiology. Regulatory, integrative and comparative physiology 310.11 (2016 Jun 1): R1020-9.

    Biochimica et biophysica acta
    Fujii NL
    Biochimica et biophysica acta 1837.10 (2014 Oct): 1699-706.

    Differentiation; research in biological diversity
    Sartore S
    Differentiation; research in biological diversity 38.1 (1988 Jun): 49-59.

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