BF-32

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$50.00
SKU: BF-32

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Available: 1

DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: BF-32
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: MYH2
Ab Isotype: MIgM
Gene Name:
Antibody Registry ID: AB_10573811 
Uniprot ID: Q9BE41 
RRID:  
Entrez Gene ID: 788772 
Clonality: Monoclonal
Immunogen: purified myosin from bovine skeletal muscle (fetal, 3 months)
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: NS0
Epitope Mapped: No
Antigen Name: Myosin heavy chain (slow and Type IIA)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 3/25/2009
Antigen Molecular Weight: 220kDa
Depositor: Schiaffino, S.
Antigen Sequence:
Depositor Institution: Universita degli Studi di Padova
Antigen Species: Bovine
Depositor Notes: Fusion: 1984. Recognizes type 1 (slow) and 2A fibers in skeletal muscle.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human, Ovine, Rodent, Sheep
Additional Information: This antibody may recognize more than one myosin isoform depending on the tissue, developmental stage, or species. The gene, Uniprot ID and gene ID associated with this mAb are not exclusive.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
BF-32 was deposited to the DSHB by Schiaffino, S. (DSHB Hybridoma Product BF-32)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

9 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication

    Three myosin heavy chain isoforms in type 2 skeletal muscle fibres.
    Lømo T
    Journal of muscle research and cell motility 10.3 (1989 Jun): 197-205.

    IF References
    WB References

    Three myosin heavy chain isoforms in type 2 skeletal muscle fibres.
    Lømo T
    Journal of muscle research and cell motility 10.3 (1989 Jun): 197-205.

    Neonatal myosin heavy chains are not expressed in Ni-induced rat rhabdomyosarcoma.
    Sartore S
    Differentiation; research in biological diversity 38.1 (1988 Jun): 49-59.

    Technical note: Protocol for electrophoretic separation of bovine myosin heavy chain isoforms and comparison to immunohistochemistry analysis.
    Wright SA
    Journal of animal science 96.10 (2018 Sep 29): 4306-4312.

    Digastric Muscle Phenotypes of the Ts65Dn Mouse Model of Down Syndrome.
    Connor NP
    PloS one 11.6 (2016): e0158008.

    Age-related changes in isolated mouse skeletal muscle function are dependent on sex, muscle, and contractility mode.
    Tallis J
    American journal of physiology. Regulatory, integrative and comparative physiology 319.3 (2020 Sep 1): R296-R314.

    IHC References
    All References

    Three myosin heavy chain isoforms in type 2 skeletal muscle fibres.
    Lømo T
    Journal of muscle research and cell motility 10.3 (1989 Jun): 197-205.

    Technical note: Protocol for electrophoretic separation of bovine myosin heavy chain isoforms and comparison to immunohistochemistry analysis.
    Wright SA
    Journal of animal science 96.10 (2018 Sep 29): 4306-4312.

    Intrauterine growth-restricted sheep fetuses exhibit smaller hindlimb muscle fibers and lower proportions of insulin-sensitive Type I fibers near term.
    Limesand SW
    American journal of physiology. Regulatory, integrative and comparative physiology 310.11 (2016 Jun 1): R1020-9.

    Imaging mass spectrometry reveals fiber-specific distribution of acetylcarnitine and contraction-induced carnitine dynamics in rat skeletal muscles.
    Fujii NL
    Biochimica et biophysica acta 1837.10 (2014 Oct): 1699-706.

    [Rare diseases of the digestive organs and difficulties in their diagnosis].
    Vinogradova MA
    Sovetskaia meditsina .3 (1978 Mar): 149-52.

    Recovery from impaired muscle growth arises from prolonged postnatal accretion of myonuclei in Atrx mutant mice.
    Picketts DJ
    PloS one 12.11 (2017): e0186989.

    Neonatal myosin heavy chains are not expressed in Ni-induced rat rhabdomyosarcoma.
    Sartore S
    Differentiation; research in biological diversity 38.1 (1988 Jun): 49-59.

    Digastric Muscle Phenotypes of the Ts65Dn Mouse Model of Down Syndrome.
    Connor NP
    PloS one 11.6 (2016): e0158008.

    Age-related changes in isolated mouse skeletal muscle function are dependent on sex, muscle, and contractility mode.
    Tallis J
    American journal of physiology. Regulatory, integrative and comparative physiology 319.3 (2020 Sep 1): R296-R314.

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