Antigen: mCherry fluorescent protein
Hybridoma Cells Available:
Antigen Species: Discosoma sp.
Isotype: MIgG1, kappa light chain
Host Species: mouse
Depositors Institution: University of Iowa
Positive Tested Species Reactivity: Discosoma sp. All species as fusion proteins.
Depositors Notes: mCherry fluoresent protein is a second generation derivative of red fluorescent protein isolated from Discosoma sp (dsRed). mCherry has improved brightness and photostability.
Antigen Molecular Weight: 28.8 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: Recombinant mCherry expressed in E. coli
Alternate Gene Names:
Alternate Antibody Name:
Alternate Antigen Name:
Myeloma Strain: Sp2/0-Ag14
Epitope Location or Sequence:
Immunogen Sequence: Full length protein
Entrez Gene ID:
Antibody Registry ID: AB_2617431
Additional Information: This antibody recognizes native mCherry and fails to recognize denatured protein. RRID:AB_2617431
Recommended Applications: Native Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DSHB-mCherry-3D5 was deposited to the DSHB by DSHB (DSHB Hybridoma Product DSHB-mCherry-3D5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).