Product Name/ID: DSHB-mCherry-3D5
Available to For-Profits: Yes
Alternate Antibody Name:
Ab Isotype: MIgG1, kappa light chain
Antibody Registry ID: AB_2617431
Immunogen: Recombinant mCherry expressed in E. coli
Immunogen Sequence: Full length protein
Myeloma Strain: Sp2/0-Ag14
Epitope Mapped: No
Antigen Name: mCherry fluorescent protein
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 4/6/2016
Antigen Molecular Weight: 28.8 kDa
Depositor Institution: University of Iowa
Antigen Species: Discosoma sp.
Depositor Notes: mCherry fluoresent protein is a second generation derivative of red fluorescent protein isolated from Discosoma sp (dsRed). mCherry has improved brightness and photostability.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Discosoma sp. All species as fusion proteins.
Additional Information: This antibody recognizes native mCherry and fails to recognize denatured protein. RRID:AB_2617431
Recommended Applications: Native Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DSHB-mCherry-3D5 was deposited to the DSHB by DSHB (DSHB Hybridoma Product DSHB-mCherry-3D5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).