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SKU: DSHB-mCherry-3D5-s
View product citations for antibody DSHB-mCherry-3D5 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: mCherry fluorescent protein
Depositor: DSHB
Antigen Species: Discosoma sp.
Depositors Institution: University of Iowa
Isotype: MIgG1, kappa light chain
Depositors Notes: mCherry fluoresent protein is a second generation derivative of red fluorescent protein isolated from Discosoma sp (dsRed). mCherry has improved brightness and photostability.
Host Species: mouse
Clonality: Monoclonal
Positive Tested Species Reactivity: Discosoma sp. All species as fusion proteins.
Immunogen Sequence: Full length protein
Antigen Molecular Weight: 28.8 kDa
Additional Information: This antibody recognizes native mCherry and fails to recognize denatured protein. RRID:AB_2617431
Immunogen: Recombinant mCherry expressed in E. coli
Myeloma Strain: Sp2/0-Ag14
Antibody Registry ID: AB_2617431 
Recommended Applications: Native Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DSHB-mCherry-3D5 was deposited to the DSHB by DSHB (DSHB Hybridoma Product DSHB-mCherry-3D5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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