DSHB-mCherry-3A11

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$40.00
SKU: DSHB-mCherry-3A11-s
View product citations for antibody DSHB-mCherry-3A11 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: mCherry fluorescent protein
Hybridoma Cells Available: No
Antigen Species: Discosoma sp.
Depositor: DSHB
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Iowa
Positive Tested Species Reactivity: Discosoma sp. All species as fusion proteins.
Depositors Notes: mCherry fluoresent protein is a monomeric derivative of red fluorescent protein isolated from Discosoma sp (dsRed). mCherry has improved brightness and photostability.
Antigen Molecular Weight: 28.8 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: Recombinant mCherry expressed in E. coli
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: Sp2/0-Ag14
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence: Full length protein
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_2617430 
Additional Information: This antibody recognizes both denatured and native mCherry. RRID:AB_2617430
Recommended Applications: Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DSHB-mCherry-3A11 was deposited to the DSHB by DSHB (DSHB Hybridoma Product DSHB-mCherry-3A11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

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