Antigen: Delta, extracellular domain
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Artavanis-Tsakonas, S.
Host Species: mouse
Depositors Institution: Harvard Medical School
Positive Tested Species Reactivity: Drosophila
Antigen Molecular Weight: Predicted: 89 kDa; Apparent: full length and 65 kDa proteolytic extracellular fragment.
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: Drosophila Delta extracellular domain
Alternate Gene Names: CG3619
Alternate Antibody Name: 9B, MAb202, anti-Delta9B
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: Ag8
Epitope Location or Sequence: EGF-like repeats 4 and/or 5
Uniprot ID: P10041
Immunogen Sequence: Partial protein
Entrez Gene ID: 42313
Antibody Registry ID: AB_528194
Additional Information: The extracellular fragment of Delta is a ligand for Notch
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
C594.9B was deposited to the DSHB by Artavanis-Tsakonas, S. (DSHB Hybridoma Product C594.9B)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
Epitope Map References