C594.9B

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$40.00
SKU: C594.9B
View product citations for antibody C594.9B on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Delta, extracellular domain
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Artavanis-Tsakonas, S.
Isotype: MIgG1
Depositors Institution: Harvard Medical School
Host Species: mouse
Gene: Dl
Positive Tested Species Reactivity: Drosophila
Alternate Gene Names: CG3619
Antigen Molecular Weight: Predicted: 89 kDa; Apparent: full length and 65 kDa proteolytic extracellular fragment.
Clonality: Monoclonal
Immunogen: Drosophila Delta extracellular domain
Epitope Mapped: Yes
Alternate Antibody Name: 9B, MAb202, anti-Delta9B
Epitope Location or Sequence: EGF-like repeats 4 and/or 5
Myeloma Strain: Ag8
Immunogen Sequence: Partial protein
Uniprot ID: P10041 
Additional Information: The extracellular fragment of Delta is a ligand for Notch
Entrez Gene ID: 42313 
Antibody Registry ID: AB_528194 
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
C594.9B was deposited to the DSHB by Artavanis-Tsakonas, S. (DSHB Hybridoma Product C594.9B)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

23 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication

    Processing of the notch ligand delta by the metalloprotease Kuzbanian.
    Artavanis-Tsakonas S
    Science (New York, N.Y.) 283.5398 (1999 Jan 1): 91-4.

    The intracellular deletions of Delta and Serrate define dominant negative forms of the Drosophila Notch ligands.
    Artavanis-Tsakonas S
    Development (Cambridge, England) 122.8 (1996 Aug): 2465-74.

    IF References

    Complex proteolytic processing acts on Delta, a transmembrane ligand for Notch, during Drosophila development.
    Muskavitch MA
    Molecular biology of the cell 9.7 (1998 Jul): 1709-23.

    Interplay of Notch and FGF signaling restricts cell fate and MAPK activation in the Drosophila trachea.
    Hayashi S
    Development (Cambridge, England) 126.20 (1999 Oct): 4455-63.

    Control of tracheal tubulogenesis by Wingless signaling.
    Hayashi S
    Development (Cambridge, England) 127.20 (2000 Oct): 4433-42.

    Retinoblastoma family protein promotes normal R8-photoreceptor differentiation in the absence of rhinoceros by inhibiting dE2F1 activity.
    Du W
    Developmental biology 335.1 (2009 Nov 1): 228-36.

    A Hh-driven gene network controls specification, pattern and size of the Drosophila simple eyes.
    Casares F
    Development (Cambridge, England) 140.1 (2013 Jan 1): 82-92.

    Delta expression in post-mitotic neurons identifies distinct subsets of adult-specific lineages in Drosophila.
    Rand MD
    Developmental neurobiology 67.1 (2007 Jan): 23-38.

    Endocytosis-independent mechanisms of Delta ligand proteolysis.
    Rand MD
    Experimental cell research 312.8 (2006 May 1): 1345-60.

    The intracellular deletions of Delta and Serrate define dominant negative forms of the Drosophila Notch ligands.
    Artavanis-Tsakonas S
    Development (Cambridge, England) 122.8 (1996 Aug): 2465-74.

    Kuz and TACE can activate Notch independent of ligand.
    Rand MD
    Cellular and molecular life sciences : CMLS 65.14 (2008 Jul): 2232-43.

    Ligand-receptor interactions and trans-endocytosis of Delta, Serrate and Notch: members of the Notch signalling pathway in Drosophila.
    Muskavitch MA
    Journal of cell science 112 ( Pt 19). (1999 Oct): 3289-97.

    Functional analysis of the NHR2 domain indicates that oligomerization of Neuralized regulates ubiquitination and endocytosis of Delta during Notch signaling.
    Boulianne GL
    Molecular and cellular biology 32.24 (2012 Dec): 4933-45.

    The Notch amino terminus regulates protein levels and Delta-induced clustering of Drosophila Notch receptors.
    Wesley C
    Experimental cell research 304.1 (2005 Mar 10): 202-23.

    The lipolysis pathway sustains normal and transformed stem cells in adult Drosophila.
    Hou SX
    Nature 538.7623 (2016 Oct 6): 109-113.

    Transient Scute activation via a self-stimulatory loop directs enteroendocrine cell pair specification from self-renewing intestinal stem cells.
    Xi R
    Nature cell biology 20.2 (2018 Feb): 152-161.

    Quantitative Morphological Variation in the Developing <i>Drosophila</i> Wing.
    Houle D
    G3 (Bethesda, Md.) 8.7 (2018 Jul 2): 2399-2409.

    WB References

    Processing of the notch ligand delta by the metalloprotease Kuzbanian.
    Artavanis-Tsakonas S
    Science (New York, N.Y.) 283.5398 (1999 Jan 1): 91-4.

    Complex proteolytic processing acts on Delta, a transmembrane ligand for Notch, during Drosophila development.
    Muskavitch MA
    Molecular biology of the cell 9.7 (1998 Jul): 1709-23.

    Down-regulation of Delta by proteolytic processing.
    Artavanis-Tsakonas S
    The Journal of cell biology 159.2 (2002 Oct 28): 313-24.

    Notch-induced proteolysis and nuclear localization of the Delta ligand.
    Rand MD
    The Journal of biological chemistry 278.16 (2003 Apr 18): 13607-10.

    Cytosolic interaction between deltex and Notch ankyrin repeats implicates deltex in the Notch signaling pathway.
    Artavanis-Tsakonas S
    Development (Cambridge, England) 120.3 (1994 Mar): 473-81.

    The Notch amino terminus regulates protein levels and Delta-induced clustering of Drosophila Notch receptors.
    Wesley C
    Experimental cell research 304.1 (2005 Mar 10): 202-23.

    IHC References

    Immunohistochemical examination of cytological differentiation in osteosarcomas.
    Kanda H
    European journal of medical research 10.11 (2005 Nov 16): 475-9.

    IP References
    Epitope Map References
    All References

    Immunohistochemical examination of cytological differentiation in osteosarcomas.
    Kanda H
    European journal of medical research 10.11 (2005 Nov 16): 475-9.

    Complex proteolytic processing acts on Delta, a transmembrane ligand for Notch, during Drosophila development.
    Muskavitch MA
    Molecular biology of the cell 9.7 (1998 Jul): 1709-23.

    Interplay of Notch and FGF signaling restricts cell fate and MAPK activation in the Drosophila trachea.
    Hayashi S
    Development (Cambridge, England) 126.20 (1999 Oct): 4455-63.

    Control of tracheal tubulogenesis by Wingless signaling.
    Hayashi S
    Development (Cambridge, England) 127.20 (2000 Oct): 4433-42.

    Retinoblastoma family protein promotes normal R8-photoreceptor differentiation in the absence of rhinoceros by inhibiting dE2F1 activity.
    Du W
    Developmental biology 335.1 (2009 Nov 1): 228-36.

    A Hh-driven gene network controls specification, pattern and size of the Drosophila simple eyes.
    Casares F
    Development (Cambridge, England) 140.1 (2013 Jan 1): 82-92.

    Delta expression in post-mitotic neurons identifies distinct subsets of adult-specific lineages in Drosophila.
    Rand MD
    Developmental neurobiology 67.1 (2007 Jan): 23-38.

    Endocytosis-independent mechanisms of Delta ligand proteolysis.
    Rand MD
    Experimental cell research 312.8 (2006 May 1): 1345-60.

    The intracellular deletions of Delta and Serrate define dominant negative forms of the Drosophila Notch ligands.
    Artavanis-Tsakonas S
    Development (Cambridge, England) 122.8 (1996 Aug): 2465-74.

    Kuz and TACE can activate Notch independent of ligand.
    Rand MD
    Cellular and molecular life sciences : CMLS 65.14 (2008 Jul): 2232-43.

    Ligand-receptor interactions and trans-endocytosis of Delta, Serrate and Notch: members of the Notch signalling pathway in Drosophila.
    Muskavitch MA
    Journal of cell science 112 ( Pt 19). (1999 Oct): 3289-97.

    Functional analysis of the NHR2 domain indicates that oligomerization of Neuralized regulates ubiquitination and endocytosis of Delta during Notch signaling.
    Boulianne GL
    Molecular and cellular biology 32.24 (2012 Dec): 4933-45.

    The Notch amino terminus regulates protein levels and Delta-induced clustering of Drosophila Notch receptors.
    Wesley C
    Experimental cell research 304.1 (2005 Mar 10): 202-23.

    The lipolysis pathway sustains normal and transformed stem cells in adult Drosophila.
    Hou SX
    Nature 538.7623 (2016 Oct 6): 109-113.

    Transient Scute activation via a self-stimulatory loop directs enteroendocrine cell pair specification from self-renewing intestinal stem cells.
    Xi R
    Nature cell biology 20.2 (2018 Feb): 152-161.

    Quantitative Morphological Variation in the Developing <i>Drosophila</i> Wing.
    Houle D
    G3 (Bethesda, Md.) 8.7 (2018 Jul 2): 2399-2409.

    Processing of the notch ligand delta by the metalloprotease Kuzbanian.
    Artavanis-Tsakonas S
    Science (New York, N.Y.) 283.5398 (1999 Jan 1): 91-4.

    Down-regulation of Delta by proteolytic processing.
    Artavanis-Tsakonas S
    The Journal of cell biology 159.2 (2002 Oct 28): 313-24.

    Notch-induced proteolysis and nuclear localization of the Delta ligand.
    Rand MD
    The Journal of biological chemistry 278.16 (2003 Apr 18): 13607-10.

    Cytosolic interaction between deltex and Notch ankyrin repeats implicates deltex in the Notch signaling pathway.
    Artavanis-Tsakonas S
    Development (Cambridge, England) 120.3 (1994 Mar): 473-81.

    Notch ligands are substrates for protein O-fucosyltransferase-1 and Fringe.
    Haltiwanger RS
    The Journal of biological chemistry 277.33 (2002 Aug 16): 29945-52.

    Cullin-3 regulates pattern formation, external sensory organ development and cell survival during Drosophila development.
    Skeath JB
    Mechanisms of development 121.12 (2004 Dec): 1495-507.

    Neuralized promotes basal to apical transcytosis of delta in epithelial cells.
    Le Borgne R
    Molecular biology of the cell 21.12 (2010 Jun 15): 2078-86.

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