I12 anti-Hoxb4

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$45.00 to $425.00
SKU: I12 anti-Hoxb4
View product citations for antibody I12 anti-Hoxb4 on CiteAb

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Available: 135

DSHB Data Sheet

Catalog Fields

Antigen: Hoxb4
Hybridoma Cells Available: Yes
Antigen Species: mouse
Depositor: Gould, A. / Krumlauf, R.
Isotype: RIgG2a
Antigen Sequence:
Host Species: rat
Depositors Institution: MRC National Institute for Medical Research
Positive Tested Species Reactivity: Human, Mouse, Rat
Depositors Notes: Specificity note: mouse, rat and human; not chicken.
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Immunogen: Hoxb4 (mouse); bacterial GEX fusion protein
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: Ag8
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_2119288 
Additional Information:
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
I12 anti-Hoxb4 was deposited to the DSHB by Gould, A. / Krumlauf, R. (DSHB Hybridoma Product I12 anti-Hoxb4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IF References
  • All References
  • Initial Publication
    IF References

    Generation of region-specific and high-purity neurons from human feeder-free iPSCs.
    Okano H
    Neuroscience letters 746. (2021 Feb 16): 135676.

    All References

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