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$45.00 to $425.00
SKU: S58
View product citations for antibody S58 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Myosin heavy chain, slow contracting muscle
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Stockdale, F.E.
Isotype: MIgA
Antigen Sequence:
Host Species: mouse
Depositors Institution: Stanford University School of Medicine
Positive Tested Species Reactivity: Chicken, Human, Mouse, Quail, Rat, Zebrafish
Depositors Notes: In birds, this antibody recognizes myosin heavy chain isoforms SM2 and SM3, but not SM1. The antibody does not recognize aldehyde-fixed tissue. Ethanol fixation is preferred. For western blotting, 5% SDS-PAGE gels are recommended.
Antigen Molecular Weight: ~200 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: MYH7B
Immunogen: Anterior latissimus dorsi (ALD) muscle from Day 19 embryo limb
Alternate Gene Names: SM2, SSMHC, myosin-7B
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3/NS1.1Ag4-1
Epitope Location or Sequence:
Uniprot ID: E1C523 
Immunogen Sequence: Purified full length myosin
Entrez Gene ID: 395279 
Additional Characterization:  
Antibody Registry ID: AB_528377 
Additional Information: RRID:AB_528377
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
S58 was deposited to the DSHB by Stockdale, F.E. (DSHB Hybridoma Product S58)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

9 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication

    Slow and fast myosin heavy chain content defines three types of myotubes in early muscle cell cultures.
    Stockdale FE
    The Journal of cell biology 101.5 Pt 1 (1985 Nov): 1643-50.

    IF References
    WB References
    IHC References
    Epitope Map References
    All References

    Slow and fast myosin heavy chain content defines three types of myotubes in early muscle cell cultures.
    Stockdale FE
    The Journal of cell biology 101.5 Pt 1 (1985 Nov): 1643-50.

    Myosin expression and specialization among the earliest muscle fibers of the developing avian limb.
    Stockdale FE
    Developmental biology 113.1 (1986 Jan): 238-54.

    Effects of strength, endurance and combined training on myosin heavy chain content and fibre-type distribution in humans.
    Bell GJ
    European journal of applied physiology 92.4-5 (2004 Aug): 376-84.

    The miR-30 microRNA family targets smoothened to regulate hedgehog signalling in zebrafish early muscle development.
    Brook JD
    PloS one 8.6 (2013): e65170.

    The myosin co-chaperone UNC-45 is required for skeletal and cardiac muscle function in zebrafish.
    Pilgrim DB
    Developmental biology 303.2 (2007 Mar 15): 483-92.

    Schwann cells selectively myelinate primary motor axons via neuregulin-ErbB signaling.
    Park HC
    Glia 68.12 (2020 Dec): 2585-2600.

    Expression of multiple slow myosin heavy chain genes reveals a diversity of zebrafish slow twitch muscle fibres with differing requirements for Hedgehog and Prdm1 activity.
    Ingham PW
    Development (Cambridge, England) 135.12 (2008 Jun): 2115-26.

    Restoration of cardiac tissue thyroid hormone status in experimental hypothyroidism: a dose-response study in female rats.
    Gerdes AM
    Endocrinology 154.7 (2013 Jul): 2542-52.

    Developmentally regulated expression of three slow isoforms of myosin heavy chain: diversity among the first fibers to form in avian muscle.
    Stockdale FE
    Developmental biology 154.1 (1992 Nov): 118-28.

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