Antigen: Engrailed/invected gene products
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Goodman, C.
Host Species: mouse
Depositors Institution: University of California, Berkeley
Positive Tested Species Reactivity: Beetle, Chicken, Crayfish, Drosophila, Fish, Grasshopper, Lobster, Molluscs, Worm, Xenopus, Zebrafish
Depositors Notes: This antibody recognizes both the engrailed and invected gene products of Drosophila. The antibody is useful for examining the patterning of segmentation from the cellular blastoderm stage onward. Staining is also seen in a large number of CNS neurons including most of the median neuroblast progeny as well as a small number of PNS neurons. The antibody doesn't recognize engrailed in mouse, human, sea urchin and Manduca sexta (moth).
Antigen Molecular Weight: Predicted: 59 kDa for en; 61 kDa for in
Human Protein Atlas:
Predicted Species Reactivity:
Gene: en & in
Immunogen: Invected (C-terminal two-thirds of the invected protein); recombinant.
Alternate Gene Names:
Alternate Antibody Name: 4D9
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS-1
Epitope Location or Sequence: aa 38-48 of the engrailed homeobox (C-terminal aa 490-500 of engrailed: ELGLNEAQIKI; aa 507-517 of invected)
Uniprot ID: P02836 P05527
Entrez Gene ID: 36240 36239
Antibody Registry ID: AB_528224
Recommended Applications: Chromatin Immunoprecipitation, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
4D9 anti-engrailed/invected was deposited to the DSHB by Goodman, C. (DSHB Hybridoma Product 4D9 anti-engrailed/invected)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
Epitope Map References
Gel Supershift References