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4D9 anti-engrailed/invected

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SKU: 4D9 anti-engrailed/invected
View product citations for antibody 4D9 anti-engrailed/invected on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Engrailed/invected gene products
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Goodman, C.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California, Berkeley
Positive Tested Species Reactivity: Beetle, Chicken, Crayfish, Drosophila, Fish, Grasshopper, Lobster, Molluscs, Worm, Xenopus, Zebrafish
Depositors Notes: This antibody recognizes both the engrailed and invected gene products of Drosophila. The antibody is useful for examining the patterning of segmentation from the cellular blastoderm stage onward. Staining is also seen in a large number of CNS neurons including most of the median neuroblast progeny as well as a small number of PNS neurons. The antibody doesn't recognize engrailed in mouse, human, sea urchin and Manduca sexta (moth).
Antigen Molecular Weight: Predicted: 59 kDa for en; 61 kDa for in
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: en & in
Immunogen: Invected (C-terminal two-thirds of the invected protein); recombinant.
Alternate Gene Names:
Alternate Antibody Name: 4D9
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS-1
Epitope Location or Sequence: aa 38-48 of the engrailed homeobox (C-terminal aa 490-500 of engrailed: ELGLNEAQIKI; aa 507-517 of invected)
Uniprot ID: P02836 P05527 
Immunogen Sequence:
Entrez Gene ID: 36240 36239 
Additional Characterization:
Antibody Registry ID: AB_528224 
Additional Information:
Recommended Applications: Chromatin Immunoprecipitation, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
4D9 anti-engrailed/invected was deposited to the DSHB by Goodman, C. (DSHB Hybridoma Product 4D9 anti-engrailed/invected)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

25 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FB References
  • Epitope Map References
  • Gel Supershift References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    IP References
    FB References
    Epitope Map References
    Gel Supershift References
    All References

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