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4D9 anti-engrailed/invected

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$40.00
SKU: 4D9 anti-engrailed/invected
View product citations for antibody 4D9 anti-engrailed/invected on CiteAb

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Available: 126

DSHB Data Sheet

Catalog Fields

Antigen: Engrailed/invected gene products
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Goodman, C.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California, Berkeley
Positive Tested Species Reactivity: Beetle, Chicken, Crayfish, Drosophila, Fish, Grasshopper, Lobster, Molluscs, Worm, Xenopus, Zebrafish
Depositors Notes: This antibody recognizes both the engrailed and invected gene products of Drosophila. The antibody is useful for examining the patterning of segmentation from the cellular blastoderm stage onward. Staining is also seen in a large number of CNS neurons including most of the median neuroblast progeny as well as a small number of PNS neurons. The antibody doesn't recognize engrailed in mouse, human, sea urchin and Manduca sexta (moth).
Antigen Molecular Weight: Predicted: 59 kDa for en; 61 kDa for in
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: en & in
Immunogen: Invected (C-terminal two-thirds of the invected protein); recombinant.
Alternate Gene Names:
Alternate Antibody Name: 4D9
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS-1
Epitope Location or Sequence: aa 38-48 of the engrailed homeobox (C-terminal aa 490-500 of engrailed: ELGLNEAQIKI; aa 507-517 of invected)
Uniprot ID: P02836 P05527 
Immunogen Sequence:
Entrez Gene ID: 36240 36239 
Additional Characterization:
Antibody Registry ID: AB_528224 
Additional Information:
Recommended Applications: Chromatin Immunoprecipitation, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
4D9 anti-engrailed/invected was deposited to the DSHB by Goodman, C. (DSHB Hybridoma Product 4D9 anti-engrailed/invected)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

25 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FB References
  • Epitope Map References
  • Gel Supershift References
  • All References
  • Initial Publication

    Cell
    Goodman CS
    Cell 58.5 (1989 Sep 8): 955-68.

    IF References

    Development (Cambridge, England)
    Wieschaus E
    Development (Cambridge, England) 126.3 (1999 Feb): 577-86.

    Proceedings of the National Academy of Sciences of the United States of America
    Schmidt-Ott U
    Proceedings of the National Academy of Sciences of the United States of America 97.20 (2000 Sep 26): 10844-9.

    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
    Yokoyama KK
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 49.9 (2001 Sep): 1177-82.

    Developmental biology
    Harzsch S
    Developmental biology 258.1 (2003 Jun 1): 44-56.

    Developmental biology
    Verheyen EM
    Developmental biology 343.1-2 (2010 Jul 1): 178-89.

    Developmental biology
    Jaynes JB
    Developmental biology 366.2 (2012 Jun 15): 382-92.

    Development (Cambridge, England)
    Casares F
    Development (Cambridge, England) 140.1 (2013 Jan 1): 82-92.

    Development (Cambridge, England)
    Crews ST
    Development (Cambridge, England) 136.7 (2009 Apr): 1147-57.

    Neuron
    Prochiantz A
    Neuron 64.3 (2009 Nov 12): 355-366.

    Development (Cambridge, England)
    Maschat F
    Development (Cambridge, England) 121.6 (1995 Jun): 1691-703.

    Scientific reports
    Shashidhara LS
    Scientific reports 6. (2016 Jun 14): 27885.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Ruggiero F
    The Journal of neuroscience : the official journal of the Society for Neuroscience 36.9 (2016 Mar 2): 2663-76.

    Developmental biology
    Piotrowski T
    Developmental biology 419.2 (2016 Nov 15): 321-335.

    Developmental biology
    Geyer PK
    Developmental biology 415.1 (2016 Jul 1): 75-86.

    Development (Cambridge, England)
    Doe CQ
    Development (Cambridge, England) 141.12 (2014 Jun): 2524-32.

    PLoS genetics
    Liu JL
    PLoS genetics 9.2 (2013): e1003256.

    Development (Cambridge, England)
    Kumar JP
    Development (Cambridge, England) 145.7 (2018 Apr 4): .

    Development (Cambridge, England)
    Technau GM
    Development (Cambridge, England) 143.8 (2016 Apr 15): 1290-301.

    WB References

    Cell
    Goodman CS
    Cell 58.5 (1989 Sep 8): 955-68.

    IHC References

    Cell
    Goodman CS
    Cell 58.5 (1989 Sep 8): 955-68.

    Cell
    Goodman CS
    Cell 58.5 (1989 Sep 8): 955-68.

    The Journal of cell biology
    Vincent JP
    The Journal of cell biology 140.1 (1998 Jan 12): 183-95.

    Nature cell biology
    Bienz M
    Nature cell biology 1.3 (1999 Jul): 144-51.

    Proceedings of the National Academy of Sciences of the United States of America
    Schmidt-Ott U
    Proceedings of the National Academy of Sciences of the United States of America 97.20 (2000 Sep 26): 10844-9.

    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
    Yokoyama KK
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 49.9 (2001 Sep): 1177-82.

    The Journal of biological chemistry
    Furukawa K
    The Journal of biological chemistry 277.48 (2002 Nov 29): 46280-8.

    Developmental biology
    Harzsch S
    Developmental biology 258.1 (2003 Jun 1): 44-56.

    Development genes and evolution
    Bonnaud L
    Development genes and evolution 217.5 (2007 May): 353-62.

    Developmental biology
    Verheyen EM
    Developmental biology 343.1-2 (2010 Jul 1): 178-89.

    Matrix biology : journal of the International Society for Matrix Biology
    Ohtsuka A
    Matrix biology : journal of the International Society for Matrix Biology 30.4 (2011 May): 258-66.

    Scientific reports
    Shashidhara LS
    Scientific reports 6. (2016 Jun 14): 27885.

    Cell reports
    Takeda H
    Cell reports 27.3 (2019 Apr 16): 928-939.e4.

    IP References

    Development (Cambridge, England)
    Crews ST
    Development (Cambridge, England) 136.7 (2009 Apr): 1147-57.

    FB References

    Cell
    Goodman CS
    Cell 58.5 (1989 Sep 8): 955-68.

    Neuron
    Prochiantz A
    Neuron 64.3 (2009 Nov 12): 355-366.

    Epitope Map References

    Cell
    Goodman CS
    Cell 58.5 (1989 Sep 8): 955-68.

    Gel Supershift References
    All References

    Cell
    Goodman CS
    Cell 58.5 (1989 Sep 8): 955-68.

    The Journal of cell biology
    Vincent JP
    The Journal of cell biology 140.1 (1998 Jan 12): 183-95.

    Nature cell biology
    Bienz M
    Nature cell biology 1.3 (1999 Jul): 144-51.

    Proceedings of the National Academy of Sciences of the United States of America
    Schmidt-Ott U
    Proceedings of the National Academy of Sciences of the United States of America 97.20 (2000 Sep 26): 10844-9.

    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
    Yokoyama KK
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 49.9 (2001 Sep): 1177-82.

    The Journal of biological chemistry
    Furukawa K
    The Journal of biological chemistry 277.48 (2002 Nov 29): 46280-8.

    Developmental biology
    Harzsch S
    Developmental biology 258.1 (2003 Jun 1): 44-56.

    Development genes and evolution
    Bonnaud L
    Development genes and evolution 217.5 (2007 May): 353-62.

    Developmental biology
    Verheyen EM
    Developmental biology 343.1-2 (2010 Jul 1): 178-89.

    Matrix biology : journal of the International Society for Matrix Biology
    Ohtsuka A
    Matrix biology : journal of the International Society for Matrix Biology 30.4 (2011 May): 258-66.

    Scientific reports
    Shashidhara LS
    Scientific reports 6. (2016 Jun 14): 27885.

    Cell reports
    Takeda H
    Cell reports 27.3 (2019 Apr 16): 928-939.e4.

    Development (Cambridge, England)
    Wieschaus E
    Development (Cambridge, England) 126.3 (1999 Feb): 577-86.

    Developmental biology
    Jaynes JB
    Developmental biology 366.2 (2012 Jun 15): 382-92.

    Development (Cambridge, England)
    Casares F
    Development (Cambridge, England) 140.1 (2013 Jan 1): 82-92.

    Development (Cambridge, England)
    Crews ST
    Development (Cambridge, England) 136.7 (2009 Apr): 1147-57.

    Neuron
    Prochiantz A
    Neuron 64.3 (2009 Nov 12): 355-366.

    Development (Cambridge, England)
    Maschat F
    Development (Cambridge, England) 121.6 (1995 Jun): 1691-703.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Ruggiero F
    The Journal of neuroscience : the official journal of the Society for Neuroscience 36.9 (2016 Mar 2): 2663-76.

    Developmental biology
    Piotrowski T
    Developmental biology 419.2 (2016 Nov 15): 321-335.

    Developmental biology
    Geyer PK
    Developmental biology 415.1 (2016 Jul 1): 75-86.

    Development (Cambridge, England)
    Doe CQ
    Development (Cambridge, England) 141.12 (2014 Jun): 2524-32.

    PLoS genetics
    Liu JL
    PLoS genetics 9.2 (2013): e1003256.

    Development (Cambridge, England)
    Kumar JP
    Development (Cambridge, England) 145.7 (2018 Apr 4): .

    Development (Cambridge, England)
    Technau GM
    Development (Cambridge, England) 143.8 (2016 Apr 15): 1290-301.

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