Clone ID / Name: 4D9 anti-engrailed/invected

Anti - Engrailed/invected gene products

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Confocal microscope image of a Drosophila embryo labeled with DSHB antibody 4D9 (magenta). PMID: 37714829, Fig. 3G.

$50.00

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SKU: 4D9 anti-engrailed/invected

Product Form :

Supernatant 1.0 ml

~ 48 – 64 μg/mL

Frozen Cells 1 vial

N/A

Growing Cells 1 vial

N/A

iConcentration varies between lots. Lots are distributed in the order they are produced.

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Catalog Fields

Identity

Clone ID / Name: 4D9 anti-engrailed/invected

Alternate Antibody Name: 4D9

Antigen Name: Engrailed/invected gene products

Alternate Antigen Name: —

Gene Symbol: —

Gene Name: en & in

Entrez Gene ID: 36240, 36239

Antigen Sequence: —

Antigen Species: Drosophila

Antigen Mol. Weight: Predicted: 59 kDa for en; 61 kDa for in

Uniprot ID: P02836, P05527

Human Protein Atlas: —

Antibody Properties

Host Species: Mouse

Clonality: Monoclonal

Isotype: MIgG1

Myeloma Strain: NS-1

Antibody Registry / RRID: AB_528224

Available to For-Profits: Yes

Hybridoma Available: Yes
Cell lines cannot be distributed to commercial/for-profit users.

Immunogen & Epitope

Immunogen: Invected (C-terminal two-thirds of the invected protein); recombinant.

Immunogen Sequence: —

Epitope Mapped: Yes

Epitope Location / Seq: aa 38-48 of the engrailed homeobox (C-terminal aa 490-500 of engrailed: ELGLNEAQIKI; aa 507-517 of invected)

Clone: —

Additional Info: —

Add. Characterization: —

Species Reactivity

Confirmed Reactivity: Beetle, Chicken, Crayfish, Drosophila, Fish, Grasshopper, Lobster, Molluscs, Worm, Xenopus, Zebrafish

Predicted Reactivity: —

Depositor

Depositor Name: C. Goodman

Institution: University of California, Berkeley

Deposit Date: February 1, 1994

Depositor Notes: This antibody recognizes both the engrailed and invected gene products of Drosophila. The antibody is useful for examining the patterning of segmentation from the cellular blastoderm stage onward. Staining is also seen in a large number of CNS neurons including most of the median neuroblast progeny as well as a small number of PNS neurons. The antibody doesn't recognize engrailed in mouse, human, sea urchin and Manduca sexta (moth).

Recommended Applications

ChIP

Function Blocking

IHC

Each lab must optimize concentration per application and species. Full usage recommendations →

UniProt DataUniProtP02836 ↗P05527 ↗

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Could not load UniProt data for P02836

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Clone ID / Name: 4D9 anti-engrailed/invected

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