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4D9 anti-engrailed/invected

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$40.00
SKU: 4D9 anti-engrailed/invected
View product citations for antibody 4D9 anti-engrailed/invected on CiteAb

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Available: 108

DSHB Data Sheet

Catalog Fields

Antigen: Engrailed/invected gene products
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Goodman, C.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California, Berkeley
Positive Tested Species Reactivity: Beetle, Chicken, Crayfish, Drosophila, Fish, Grasshopper, Lobster, Molluscs, Worm, Xenopus, Zebrafish
Depositors Notes: This antibody recognizes both the engrailed and invected gene products of Drosophila. The antibody is useful for examining the patterning of segmentation from the cellular blastoderm stage onward. Staining is also seen in a large number of CNS neurons including most of the median neuroblast progeny as well as a small number of PNS neurons. The antibody doesn't recognize engrailed in mouse, human, sea urchin and Manduca sexta (moth).
Antigen Molecular Weight: Predicted: 59 kDa for en; 61 kDa for in
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: en & in
Immunogen: Invected (C-terminal two-thirds of the invected protein); recombinant.
Alternate Gene Names:
Alternate Antibody Name: 4D9
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS-1
Epitope Location or Sequence: aa 38-48 of the engrailed homeobox (C-terminal aa 490-500 of engrailed: ELGLNEAQIKI; aa 507-517 of invected)
Uniprot ID: P02836 P05527 
Immunogen Sequence:
Entrez Gene ID: 36240 36239 
Additional Characterization:  
Antibody Registry ID: AB_528224 
Additional Information:
Recommended Applications: Chromatin Immunoprecipitation, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
4D9 anti-engrailed/invected was deposited to the DSHB by Goodman, C. (DSHB Hybridoma Product 4D9 anti-engrailed/invected)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

25 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FB References
  • Epitope Map References
  • Gel Supershift References
  • All References
  • Initial Publication
    IF References

    Wingless signaling in the Drosophila embryo: zygotic requirements and the role of the frizzled genes.
    Wieschaus E
    Development (Cambridge, England) 126.3 (1999 Feb): 577-86.

    Function of bicoid and hunchback homologs in the basal cyclorrhaphan fly Megaselia (Phoridae).
    Schmidt-Ott U
    Proceedings of the National Academy of Sciences of the United States of America 97.20 (2000 Sep 26): 10844-9.

    Simultaneous detection of RNA and protein by in situ hybridization and immunological staining.
    Yokoyama KK
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 49.9 (2001 Sep): 1177-82.

    Evolution of identified arthropod neurons: the serotonergic system in relation to engrailed-expressing cells in the embryonic ventral nerve cord of the american lobster homarus americanus milne edwards, 1873 (malacostraca, pleocyemata, homarida).
    Harzsch S
    Developmental biology 258.1 (2003 Jun 1): 44-56.

    Nemo phosphorylates Even-skipped and promotes Eve-mediated repression of odd-skipped in even parasegments during Drosophila embryogenesis.
    Verheyen EM
    Developmental biology 343.1-2 (2010 Jul 1): 178-89.

    Engrailed cooperates directly with Extradenticle and Homothorax on a distinct class of homeodomain binding sites to repress sloppy paired.
    Jaynes JB
    Developmental biology 366.2 (2012 Jun 15): 382-92.

    A Hh-driven gene network controls specification, pattern and size of the Drosophila simple eyes.
    Casares F
    Development (Cambridge, England) 140.1 (2013 Jan 1): 82-92.

    Neurexin IV and Wrapper interactions mediate Drosophila midline glial migration and axonal ensheathment.
    Crews ST
    Development (Cambridge, England) 136.7 (2009 Apr): 1147-57.

    Extracellular Engrailed participates in the topographic guidance of retinal axons in vivo.
    Prochiantz A
    Neuron 64.3 (2009 Nov 12): 355-366.

    polyhomeotic appears to be a target of engrailed regulation in Drosophila.
    Maschat F
    Development (Cambridge, England) 121.6 (1995 Jun): 1691-703.

    A comparative genomic analysis of targets of Hox protein Ultrabithorax amongst distant insect species.
    Shashidhara LS
    Scientific reports 6. (2016 Jun 14): 27885.

    Slow Muscle Precursors Lay Down a Collagen XV Matrix Fingerprint to Guide Motor Axon Navigation.
    Ruggiero F
    The Journal of neuroscience : the official journal of the Society for Neuroscience 36.9 (2016 Mar 2): 2663-76.

    Glypican4 modulates lateral line collective cell migration non cell-autonomously.
    Piotrowski T
    Developmental biology 419.2 (2016 Nov 15): 321-335.

    Drosophila male and female germline stem cell niches require the nuclear lamina protein Otefin.
    Geyer PK
    Developmental biology 415.1 (2016 Jul 1): 75-86.

    Atlas-builder software and the eNeuro atlas: resources for developmental biology and neuroscience.
    Doe CQ
    Development (Cambridge, England) 141.12 (2014 Jun): 2524-32.

    Only one isoform of Drosophila melanogaster CTP synthase forms the cytoophidium.
    Liu JL
    PLoS genetics 9.2 (2013): e1003256.

    Polycomb group (PcG) proteins and Pax6 cooperate to inhibit <i>in vivo</i> reprogramming of the developing <i>Drosophila</i> eye.
    Kumar JP
    Development (Cambridge, England) 145.7 (2018 Apr 4): .

    Gene expression profiles uncover individual identities of gnathal neuroblasts and serial homologies in the embryonic CNS of Drosophila.
    Technau GM
    Development (Cambridge, England) 143.8 (2016 Apr 15): 1290-301.

    WB References
    IHC References

    Expression of engrailed proteins in arthropods, annelids, and chordates.
    Goodman CS
    Cell 58.5 (1989 Sep 8): 955-68.

    Expression of engrailed proteins in arthropods, annelids, and chordates.
    Goodman CS
    Cell 58.5 (1989 Sep 8): 955-68.

    Signaling and adhesion activities of mammalian beta-catenin and plakoglobin in Drosophila.
    Vincent JP
    The Journal of cell biology 140.1 (1998 Jan 12): 183-95.

    A new Drosophila APC homologue associated with adhesive zones of epithelial cells.
    Bienz M
    Nature cell biology 1.3 (1999 Jul): 144-51.

    Function of bicoid and hunchback homologs in the basal cyclorrhaphan fly Megaselia (Phoridae).
    Schmidt-Ott U
    Proceedings of the National Academy of Sciences of the United States of America 97.20 (2000 Sep 26): 10844-9.

    Simultaneous detection of RNA and protein by in situ hybridization and immunological staining.
    Yokoyama KK
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 49.9 (2001 Sep): 1177-82.

    Identification of a Drosophila gene encoding xylosylprotein beta4-galactosyltransferase that is essential for the synthesis of glycosaminoglycans and for morphogenesis.
    Furukawa K
    The Journal of biological chemistry 277.48 (2002 Nov 29): 46280-8.

    Evolution of identified arthropod neurons: the serotonergic system in relation to engrailed-expressing cells in the embryonic ventral nerve cord of the american lobster homarus americanus milne edwards, 1873 (malacostraca, pleocyemata, homarida).
    Harzsch S
    Developmental biology 258.1 (2003 Jun 1): 44-56.

    Engrailed in cephalopods: a key gene related to the emergence of morphological novelties.
    Bonnaud L
    Development genes and evolution 217.5 (2007 May): 353-62.

    Nemo phosphorylates Even-skipped and promotes Eve-mediated repression of odd-skipped in even parasegments during Drosophila embryogenesis.
    Verheyen EM
    Developmental biology 343.1-2 (2010 Jul 1): 178-89.

    Drosophila type XV/XVIII collagen, Mp, is involved in Wingless distribution.
    Ohtsuka A
    Matrix biology : journal of the International Society for Matrix Biology 30.4 (2011 May): 258-66.

    A comparative genomic analysis of targets of Hox protein Ultrabithorax amongst distant insect species.
    Shashidhara LS
    Scientific reports 6. (2016 Jun 14): 27885.

    Horizontal Boundary Cells, a Special Group of Somitic Cells, Play Crucial Roles in the Formation of Dorsoventral Compartments in Teleost Somite.
    Takeda H
    Cell reports 27.3 (2019 Apr 16): 928-939.e4.

    IP References
    FB References
    Epitope Map References
    Gel Supershift References
    All References

    Expression of engrailed proteins in arthropods, annelids, and chordates.
    Goodman CS
    Cell 58.5 (1989 Sep 8): 955-68.

    Signaling and adhesion activities of mammalian beta-catenin and plakoglobin in Drosophila.
    Vincent JP
    The Journal of cell biology 140.1 (1998 Jan 12): 183-95.

    A new Drosophila APC homologue associated with adhesive zones of epithelial cells.
    Bienz M
    Nature cell biology 1.3 (1999 Jul): 144-51.

    Function of bicoid and hunchback homologs in the basal cyclorrhaphan fly Megaselia (Phoridae).
    Schmidt-Ott U
    Proceedings of the National Academy of Sciences of the United States of America 97.20 (2000 Sep 26): 10844-9.

    Simultaneous detection of RNA and protein by in situ hybridization and immunological staining.
    Yokoyama KK
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 49.9 (2001 Sep): 1177-82.

    Identification of a Drosophila gene encoding xylosylprotein beta4-galactosyltransferase that is essential for the synthesis of glycosaminoglycans and for morphogenesis.
    Furukawa K
    The Journal of biological chemistry 277.48 (2002 Nov 29): 46280-8.

    Evolution of identified arthropod neurons: the serotonergic system in relation to engrailed-expressing cells in the embryonic ventral nerve cord of the american lobster homarus americanus milne edwards, 1873 (malacostraca, pleocyemata, homarida).
    Harzsch S
    Developmental biology 258.1 (2003 Jun 1): 44-56.

    Engrailed in cephalopods: a key gene related to the emergence of morphological novelties.
    Bonnaud L
    Development genes and evolution 217.5 (2007 May): 353-62.

    Nemo phosphorylates Even-skipped and promotes Eve-mediated repression of odd-skipped in even parasegments during Drosophila embryogenesis.
    Verheyen EM
    Developmental biology 343.1-2 (2010 Jul 1): 178-89.

    Drosophila type XV/XVIII collagen, Mp, is involved in Wingless distribution.
    Ohtsuka A
    Matrix biology : journal of the International Society for Matrix Biology 30.4 (2011 May): 258-66.

    A comparative genomic analysis of targets of Hox protein Ultrabithorax amongst distant insect species.
    Shashidhara LS
    Scientific reports 6. (2016 Jun 14): 27885.

    Horizontal Boundary Cells, a Special Group of Somitic Cells, Play Crucial Roles in the Formation of Dorsoventral Compartments in Teleost Somite.
    Takeda H
    Cell reports 27.3 (2019 Apr 16): 928-939.e4.

    Wingless signaling in the Drosophila embryo: zygotic requirements and the role of the frizzled genes.
    Wieschaus E
    Development (Cambridge, England) 126.3 (1999 Feb): 577-86.

    Engrailed cooperates directly with Extradenticle and Homothorax on a distinct class of homeodomain binding sites to repress sloppy paired.
    Jaynes JB
    Developmental biology 366.2 (2012 Jun 15): 382-92.

    A Hh-driven gene network controls specification, pattern and size of the Drosophila simple eyes.
    Casares F
    Development (Cambridge, England) 140.1 (2013 Jan 1): 82-92.

    Neurexin IV and Wrapper interactions mediate Drosophila midline glial migration and axonal ensheathment.
    Crews ST
    Development (Cambridge, England) 136.7 (2009 Apr): 1147-57.

    Extracellular Engrailed participates in the topographic guidance of retinal axons in vivo.
    Prochiantz A
    Neuron 64.3 (2009 Nov 12): 355-366.

    polyhomeotic appears to be a target of engrailed regulation in Drosophila.
    Maschat F
    Development (Cambridge, England) 121.6 (1995 Jun): 1691-703.

    Slow Muscle Precursors Lay Down a Collagen XV Matrix Fingerprint to Guide Motor Axon Navigation.
    Ruggiero F
    The Journal of neuroscience : the official journal of the Society for Neuroscience 36.9 (2016 Mar 2): 2663-76.

    Glypican4 modulates lateral line collective cell migration non cell-autonomously.
    Piotrowski T
    Developmental biology 419.2 (2016 Nov 15): 321-335.

    Drosophila male and female germline stem cell niches require the nuclear lamina protein Otefin.
    Geyer PK
    Developmental biology 415.1 (2016 Jul 1): 75-86.

    Atlas-builder software and the eNeuro atlas: resources for developmental biology and neuroscience.
    Doe CQ
    Development (Cambridge, England) 141.12 (2014 Jun): 2524-32.

    Only one isoform of Drosophila melanogaster CTP synthase forms the cytoophidium.
    Liu JL
    PLoS genetics 9.2 (2013): e1003256.

    Polycomb group (PcG) proteins and Pax6 cooperate to inhibit <i>in vivo</i> reprogramming of the developing <i>Drosophila</i> eye.
    Kumar JP
    Development (Cambridge, England) 145.7 (2018 Apr 4): .

    Gene expression profiles uncover individual identities of gnathal neuroblasts and serial homologies in the embryonic CNS of Drosophila.
    Technau GM
    Development (Cambridge, England) 143.8 (2016 Apr 15): 1290-301.

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