S46

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$40.00
SKU: S46
View product citations for antibody S46 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Myosin heavy chain, slow developmental (sd-MyHC)
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Stockdale, F.E.
Isotype: MIgG1, kappa light chain
Depositors Institution: Stanford University School of Medicine
Host Species: mouse
Depositors Notes: This antigen is also known as myosin heavy chain, cardiac muscle alpha isoform. S46 preferentially stains slow tonic myosin heavy chain in humans including extraocular, intrafusal and the atrium of the heart.
Positive Tested Species Reactivity: Chicken, Human, Mouse, Quail, Rat, Zebrafish
Gene: MYH6
Antigen Molecular Weight: 223.7 kDa
Alternate Gene Names: MYHCA, CMY6
Immunogen: Purified myosin from Pectoralis major
Clonality: Monoclonal
Myeloma Strain: P3/NS1.1Ag4-1
Epitope Mapped: Yes
Uniprot ID: P13533 
Epitope Location or Sequence: Mab S46 recognizes an epitope of atrium-specific myosin heavy, Myh6.
Entrez Gene ID: 4624 
Immunogen Sequence: Full length protein
Antibody Registry ID: AB_528376 
Additional Information: RRID:AB_528376)
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
S46 was deposited to the DSHB by Stockdale, F.E. (DSHB Hybridoma Product S46)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

6 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication

    Slow and fast myosin heavy chain content defines three types of myotubes in early muscle cell cultures.
    Stockdale FE
    The Journal of cell biology 101.5 Pt 1 (1985 Nov): 1643-50.

    IF References

    Mutation of weak atrium/atrial myosin heavy chain disrupts atrial function and influences ventricular morphogenesis in zebrafish.
    Yelon D
    Development (Cambridge, England) 130.24 (2003 Dec): 6121-9.

    Slow and fast myosin heavy chain content defines three types of myotubes in early muscle cell cultures.
    Stockdale FE
    The Journal of cell biology 101.5 Pt 1 (1985 Nov): 1643-50.

    Patterning the zebrafish heart tube: acquisition of anteroposterior polarity.
    Fishman MC
    Developmental biology 153.1 (1992 Sep): 91-101.

    Impact of Aging on Proprioceptive Sensory Neurons and Intrafusal Muscle Fibers in Mice.
    Valdez G
    The journals of gerontology. Series A, Biological sciences and medical sciences 72.6 (2017 Jun 1): 771-779.

    WB References

    Limited expression of slow tonic myosin heavy chain in human cranial muscles.
    Burkholder TJ
    Muscle & nerve 36.2 (2007 Aug): 183-9.

    Slow and fast myosin heavy chain content defines three types of myotubes in early muscle cell cultures.
    Stockdale FE
    The Journal of cell biology 101.5 Pt 1 (1985 Nov): 1643-50.

    IHC References

    Limited expression of slow tonic myosin heavy chain in human cranial muscles.
    Burkholder TJ
    Muscle & nerve 36.2 (2007 Aug): 183-9.

    Slow and fast myosin heavy chain content defines three types of myotubes in early muscle cell cultures.
    Stockdale FE
    The Journal of cell biology 101.5 Pt 1 (1985 Nov): 1643-50.

    The myosin co-chaperone UNC-45 is required for skeletal and cardiac muscle function in zebrafish.
    Pilgrim DB
    Developmental biology 303.2 (2007 Mar 15): 483-92.

    Epitope Map References
    All References

    Limited expression of slow tonic myosin heavy chain in human cranial muscles.
    Burkholder TJ
    Muscle & nerve 36.2 (2007 Aug): 183-9.

    Slow and fast myosin heavy chain content defines three types of myotubes in early muscle cell cultures.
    Stockdale FE
    The Journal of cell biology 101.5 Pt 1 (1985 Nov): 1643-50.

    The myosin co-chaperone UNC-45 is required for skeletal and cardiac muscle function in zebrafish.
    Pilgrim DB
    Developmental biology 303.2 (2007 Mar 15): 483-92.

    Mutation of weak atrium/atrial myosin heavy chain disrupts atrial function and influences ventricular morphogenesis in zebrafish.
    Yelon D
    Development (Cambridge, England) 130.24 (2003 Dec): 6121-9.

    Patterning the zebrafish heart tube: acquisition of anteroposterior polarity.
    Fishman MC
    Developmental biology 153.1 (1992 Sep): 91-101.

    Impact of Aging on Proprioceptive Sensory Neurons and Intrafusal Muscle Fibers in Mice.
    Valdez G
    The journals of gerontology. Series A, Biological sciences and medical sciences 72.6 (2017 Jun 1): 771-779.

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