S46

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SKU: S46

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DSHB Data Sheet

Catalog Fields

Product Name/ID: S46
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: MYH6
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_528376 
Uniprot ID: P13533 
RRID:  
Entrez Gene ID: 4624 
Clonality: Monoclonal
Immunogen: Purified myosin from Pectoralis major
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: P3/NS1.1Ag4-1
Epitope Mapped: Yes
Antigen Name: Myosin heavy chain, slow developmental (sd-MyHC)
Epitope Location or Sequence: Mab S46 recognizes an epitope of atrium-specific myosin heavy, Myh6.
Alternate Antigen Name:
Deposit Date: 2/12/2004
Antigen Molecular Weight: 223.7 kDa
Depositor: Stockdale, F.E.
Antigen Sequence:
Depositor Institution: Stanford University School of Medicine
Antigen Species: Chicken
Depositor Notes: This antigen is also known as myosin heavy chain, cardiac muscle alpha isoform. S46 preferentially stains slow tonic myosin heavy chain in humans including extraocular, intrafusal and the atrium of the heart.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Chicken, Human, Mouse, Quail, Rat, Zebrafish
Additional Information: RRID:AB_528376)
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
S46 was deposited to the DSHB by Stockdale, F.E. (DSHB Hybridoma Product S46)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

7 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication

    Slow and fast myosin heavy chain content defines three types of myotubes in early muscle cell cultures.
    Stockdale FE
    The Journal of cell biology 101.5 Pt 1 (1985 Nov): 1643-50.

    IF References

    Mutation of weak atrium/atrial myosin heavy chain disrupts atrial function and influences ventricular morphogenesis in zebrafish.
    Yelon D
    Development (Cambridge, England) 130.24 (2003 Dec): 6121-9.

    Slow and fast myosin heavy chain content defines three types of myotubes in early muscle cell cultures.
    Stockdale FE
    The Journal of cell biology 101.5 Pt 1 (1985 Nov): 1643-50.

    Patterning the zebrafish heart tube: acquisition of anteroposterior polarity.
    Fishman MC
    Developmental biology 153.1 (1992 Sep): 91-101.

    Impact of Aging on Proprioceptive Sensory Neurons and Intrafusal Muscle Fibers in Mice.
    Valdez G
    The journals of gerontology. Series A, Biological sciences and medical sciences 72.6 (2017 Jun 1): 771-779.

    WB References

    Limited expression of slow tonic myosin heavy chain in human cranial muscles.
    Burkholder TJ
    Muscle & nerve 36.2 (2007 Aug): 183-9.

    Slow and fast myosin heavy chain content defines three types of myotubes in early muscle cell cultures.
    Stockdale FE
    The Journal of cell biology 101.5 Pt 1 (1985 Nov): 1643-50.

    IHC References
    Epitope Map References
    All References

    Limited expression of slow tonic myosin heavy chain in human cranial muscles.
    Burkholder TJ
    Muscle & nerve 36.2 (2007 Aug): 183-9.

    Slow and fast myosin heavy chain content defines three types of myotubes in early muscle cell cultures.
    Stockdale FE
    The Journal of cell biology 101.5 Pt 1 (1985 Nov): 1643-50.

    The myosin co-chaperone UNC-45 is required for skeletal and cardiac muscle function in zebrafish.
    Pilgrim DB
    Developmental biology 303.2 (2007 Mar 15): 483-92.

    Molecular Phenotyping of White Striping and Wooden Breast Myopathies in Chicken.
    Berri C
    Frontiers in physiology 11. (2020): 633.

    Mutation of weak atrium/atrial myosin heavy chain disrupts atrial function and influences ventricular morphogenesis in zebrafish.
    Yelon D
    Development (Cambridge, England) 130.24 (2003 Dec): 6121-9.

    Patterning the zebrafish heart tube: acquisition of anteroposterior polarity.
    Fishman MC
    Developmental biology 153.1 (1992 Sep): 91-101.

    Impact of Aging on Proprioceptive Sensory Neurons and Intrafusal Muscle Fibers in Mice.
    Valdez G
    The journals of gerontology. Series A, Biological sciences and medical sciences 72.6 (2017 Jun 1): 771-779.

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