F310

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$50.00 to $600.00
SKU: F310

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DSHB Data Sheet

Catalog Fields

Product Name/ID: F310
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name:
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_531863 
Uniprot ID:  
RRID:  
Entrez Gene ID:  
Clonality:
Immunogen:
Clone:
Immunogen Sequence:
Myeloma Strain:
Epitope Mapped: No
Antigen Name: Myosin light chain 1 slow and 3 fast (LC1f/3f)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 3/17/2004
Antigen Molecular Weight:
Depositor: Stockdale, F.E.
Antigen Sequence:
Depositor Institution: Stanford University School of Medicine
Antigen Species: chicken
Depositor Notes: works well on Western blots; works well on ethanol-fixed tissue.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Chicken, Fish, Guinea Pig, Mouse, Quail, Rat, Zebrafish
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: FFPE, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
F310 was deposited to the DSHB by Stockdale, F.E. (DSHB Hybridoma Product F310)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

13 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FFPE References
  • All References
  • Initial Publication

    The role of the SPT6 chromatin remodeling factor in zebrafish embryogenesis.
    Sirotkin HI
    Developmental biology 307.2 (2007 Jul 15): 214-26.

    IF References
    WB References
    IHC References

    The Popeye domain containing 2 (popdc2) gene in zebrafish is required for heart and skeletal muscle development.
    Brand T
    Developmental biology 363.2 (2012 Mar 15): 438-50.

    Chlorpyrifos-oxon disrupts zebrafish axonal growth and motor behavior.
    Lein PJ
    Toxicological sciences : an official journal of the Society of Toxicology 121.1 (2011 May): 146-59.

    Dok-7 promotes slow muscle integrity as well as neuromuscular junction formation in a zebrafish model of congenital myasthenic syndromes.
    Lochmüller H
    Human molecular genetics 19.9 (2010 May 1): 1726-40.

    Uncoupling nicotine mediated motoneuron axonal pathfinding errors and muscle degeneration in zebrafish.
    Svoboda KR
    Toxicology and applied pharmacology 237.1 (2009 May 15): 29-40.

    Embryonic motor activity and implications for regulating motoneuron axonal pathfinding in zebrafish.
    Svoboda KR
    The European journal of neuroscience 28.6 (2008 Sep): 1080-96.

    The role of the SPT6 chromatin remodeling factor in zebrafish embryogenesis.
    Sirotkin HI
    Developmental biology 307.2 (2007 Jul 15): 214-26.

    Intracellular Calcium Mobilization Is Required for Sonic Hedgehog Signaling.
    Grunwald DJ
    Developmental cell 45.4 (2018 May 21): 512-525.e5.

    Schwann cells selectively myelinate primary motor axons via neuregulin-ErbB signaling.
    Park HC
    Glia 68.12 (2020 Dec): 2585-2600.

    A novel zebrafish model for intermediate type spinal muscular atrophy demonstrates importance of Smn for maintenance of mature motor neurons.
    Winkler C
    Human molecular genetics 30.24 (2021 Nov 30): 2488-2502.

    FFPE References
    All References

    The Popeye domain containing 2 (popdc2) gene in zebrafish is required for heart and skeletal muscle development.
    Brand T
    Developmental biology 363.2 (2012 Mar 15): 438-50.

    Chlorpyrifos-oxon disrupts zebrafish axonal growth and motor behavior.
    Lein PJ
    Toxicological sciences : an official journal of the Society of Toxicology 121.1 (2011 May): 146-59.

    Dok-7 promotes slow muscle integrity as well as neuromuscular junction formation in a zebrafish model of congenital myasthenic syndromes.
    Lochmüller H
    Human molecular genetics 19.9 (2010 May 1): 1726-40.

    Uncoupling nicotine mediated motoneuron axonal pathfinding errors and muscle degeneration in zebrafish.
    Svoboda KR
    Toxicology and applied pharmacology 237.1 (2009 May 15): 29-40.

    Embryonic motor activity and implications for regulating motoneuron axonal pathfinding in zebrafish.
    Svoboda KR
    The European journal of neuroscience 28.6 (2008 Sep): 1080-96.

    The role of the SPT6 chromatin remodeling factor in zebrafish embryogenesis.
    Sirotkin HI
    Developmental biology 307.2 (2007 Jul 15): 214-26.

    Intracellular Calcium Mobilization Is Required for Sonic Hedgehog Signaling.
    Grunwald DJ
    Developmental cell 45.4 (2018 May 21): 512-525.e5.

    Schwann cells selectively myelinate primary motor axons via neuregulin-ErbB signaling.
    Park HC
    Glia 68.12 (2020 Dec): 2585-2600.

    A novel zebrafish model for intermediate type spinal muscular atrophy demonstrates importance of Smn for maintenance of mature motor neurons.
    Winkler C
    Human molecular genetics 30.24 (2021 Nov 30): 2488-2502.

    The stress responsive gene ankrd1a is dynamically regulated during skeletal muscle development and upregulated following cardiac injury in border zone cardiomyocytes in adult zebrafish.
    Kojic S
    Gene 792. (2021 Aug 5): 145725.

    Pharmacological inhibition of GSK-3 in a guinea pig model of LPS-induced pulmonary inflammation: II. Effects on skeletal muscle atrophy.
    Langen RC
    Respiratory research 14.1 (2013 Nov 1): 117.

    Chronic Alcohol Consumption, but not Acute Intoxication, Decreases In Vitro Skeletal Muscle Contractile Function.
    Lang CH
    Alcoholism, clinical and experimental research 43.10 (2019 Oct): 2090-2099.

    Slc22a5 haploinsufficiency does not aggravate the phenotype of the long-chain acyl-CoA dehydrogenase KO mouse.
    Houten SM
    Journal of inherited metabolic disease 43.3 (2020 May): 486-495.

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