F310

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$40.00
SKU: F310
View product citations for antibody F310 on CiteAb

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Available: 187

DSHB Data Sheet

Catalog Fields

Antigen: myosin light chain 1 and 3f (LC1f/3f)
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Stockdale, F.E.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Stanford University School of Medicine
Positive Tested Species Reactivity: Chicken, Fish, Guinea Pig, Mouse, Quail, Rat, Zebrafish
Depositors Notes: works well on Western blots; works well on ethanol-fixed tissue.
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen:
Alternate Gene Names:
Alternate Antibody Name:
Clonality:
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain:
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID:  
Additional Information:
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
F310 was deposited to the DSHB by Stockdale, F.E. (DSHB Hybridoma Product F310)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

9 References

  • Initial Publication
  • WB References
  • IHC References
  • All References
  • Initial Publication

    The role of the SPT6 chromatin remodeling factor in zebrafish embryogenesis.
    Sirotkin HI
    Developmental biology 307.2 (2007 Jul 15): 214-26.

    WB References
    IHC References

    The Popeye domain containing 2 (popdc2) gene in zebrafish is required for heart and skeletal muscle development.
    Brand T
    Developmental biology 363.2 (2012 Mar 15): 438-50.

    Chlorpyrifos-oxon disrupts zebrafish axonal growth and motor behavior.
    Lein PJ
    Toxicological sciences : an official journal of the Society of Toxicology 121.1 (2011 May): 146-59.

    Dok-7 promotes slow muscle integrity as well as neuromuscular junction formation in a zebrafish model of congenital myasthenic syndromes.
    Lochmüller H
    Human molecular genetics 19.9 (2010 May 1): 1726-40.

    Uncoupling nicotine mediated motoneuron axonal pathfinding errors and muscle degeneration in zebrafish.
    Svoboda KR
    Toxicology and applied pharmacology 237.1 (2009 May 15): 29-40.

    Embryonic motor activity and implications for regulating motoneuron axonal pathfinding in zebrafish.
    Svoboda KR
    The European journal of neuroscience 28.6 (2008 Sep): 1080-96.

    The role of the SPT6 chromatin remodeling factor in zebrafish embryogenesis.
    Sirotkin HI
    Developmental biology 307.2 (2007 Jul 15): 214-26.

    Intracellular Calcium Mobilization Is Required for Sonic Hedgehog Signaling.
    Grunwald DJ
    Developmental cell 45.4 (2018 May 21): 512-525.e5.

    All References

    The Popeye domain containing 2 (popdc2) gene in zebrafish is required for heart and skeletal muscle development.
    Brand T
    Developmental biology 363.2 (2012 Mar 15): 438-50.

    Chlorpyrifos-oxon disrupts zebrafish axonal growth and motor behavior.
    Lein PJ
    Toxicological sciences : an official journal of the Society of Toxicology 121.1 (2011 May): 146-59.

    Dok-7 promotes slow muscle integrity as well as neuromuscular junction formation in a zebrafish model of congenital myasthenic syndromes.
    Lochmüller H
    Human molecular genetics 19.9 (2010 May 1): 1726-40.

    Uncoupling nicotine mediated motoneuron axonal pathfinding errors and muscle degeneration in zebrafish.
    Svoboda KR
    Toxicology and applied pharmacology 237.1 (2009 May 15): 29-40.

    Embryonic motor activity and implications for regulating motoneuron axonal pathfinding in zebrafish.
    Svoboda KR
    The European journal of neuroscience 28.6 (2008 Sep): 1080-96.

    The role of the SPT6 chromatin remodeling factor in zebrafish embryogenesis.
    Sirotkin HI
    Developmental biology 307.2 (2007 Jul 15): 214-26.

    Intracellular Calcium Mobilization Is Required for Sonic Hedgehog Signaling.
    Grunwald DJ
    Developmental cell 45.4 (2018 May 21): 512-525.e5.

    Pharmacological inhibition of GSK-3 in a guinea pig model of LPS-induced pulmonary inflammation: II. Effects on skeletal muscle atrophy.
    Langen RC
    Respiratory research 14. (2013 Nov 1): 117.

    Chronic Alcohol Consumption, but not Acute Intoxication, Decreases In Vitro Skeletal Muscle Contractile Function.
    Lang CH
    Alcoholism, clinical and experimental research 43.10 (2019 Oct): 2090-2099.

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