1E12

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$40.00
SKU: 1E12
View product citations for antibody 1E12 on CiteAb

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Available: 177

DSHB Data Sheet

Catalog Fields

Antigen: alpha-actinin, smooth muscle-specific
Hybridoma Cells Available: Yes
Antigen Species: Quail
Depositor: Little, C.D.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Kansas Medical Center
Positive Tested Species Reactivity: Canine, Chicken, Dogfish, Human, Monkey, Mouse, Quail, Shark
Depositors Notes: Before western blotting IgM with 0.5% SDS was used. Amino acid sequence analysis is positive and purification is positive.
Antigen Molecular Weight: 103 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: ACTN1
Immunogen: Crude homogenate of embryonic dorsal aorta
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain:
Epitope Location or Sequence:
Uniprot ID: P12814 
Immunogen Sequence: Full length sequence
Entrez Gene ID: 87 
Additional Characterization:
Antibody Registry ID: AB_2289065 
Additional Information: RRID:AB_2289065
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1E12 was deposited to the DSHB by Little, C.D. (DSHB Hybridoma Product 1E12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

14 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References

    Development of the aortic vessel wall as defined by vascular smooth muscle and extracellular matrix markers.
    Little CD
    Developmental biology 178.2 (1996 Sep 15): 375-92.

    Origin and differentiation of human and murine stroma.
    Charbord P
    Stem cells (Dayton, Ohio) 20.3 (2002): 205-14.

    Identification of a novel marker for primordial smooth muscle and its differential expression pattern in contractile vs noncontractile cells.
    Little CD
    The Journal of cell biology 137.4 (1997 May 19): 925-37.

    Immunohistochemical evidence for a mesothelial contribution to the ventral wall of the avian aorta.
    Muñoz-Chápuli R
    The Histochemical journal 31.12 (1999 Dec): 771-9.

    Differentiation of the smooth muscle cell phenotypes during embryonic development of coronary vessels in the rat.
    Krämer J
    Histochemistry and cell biology 116.1 (2001 Jul): 79-87.

    Proliferation and differentiation of smooth muscle cell precursors occurs simultaneously during the development of the vessel wall.
    Iruela-Arispe ML
    Developmental dynamics : an official publication of the American Association of Anatomists 209.4 (1997 Aug): 342-52.

    Molecular cloning, functional expression, and tissue distribution of a novel human gap junction-forming protein, connexin-31.9. Interaction with zona occludens protein-1.
    Kumar NM
    The Journal of biological chemistry 277.41 (2002 Oct 11): 38272-83.

    CRISPR/Cas9-based Pten knock-out and Sleeping Beauty Transposon-mediated Nras knock-in induces hepatocellular carcinoma and hepatic lipid accumulation in mice.
    Liu D
    Cancer biology and therapy 18.7 (2017 Jul 3): 505-512.

    Atrial natriuretic peptide inhibits cell cycle activity of embryonic cardiac progenitor cells via its NPRA receptor signaling axis.
    Pasumarthi KB
    American journal of physiology. Cell physiology 308.7 (2015 Apr 1): C557-69.

    All References

    Development of the aortic vessel wall as defined by vascular smooth muscle and extracellular matrix markers.
    Little CD
    Developmental biology 178.2 (1996 Sep 15): 375-92.

    Origin and differentiation of human and murine stroma.
    Charbord P
    Stem cells (Dayton, Ohio) 20.3 (2002): 205-14.

    Identification of a novel marker for primordial smooth muscle and its differential expression pattern in contractile vs noncontractile cells.
    Little CD
    The Journal of cell biology 137.4 (1997 May 19): 925-37.

    Immunohistochemical evidence for a mesothelial contribution to the ventral wall of the avian aorta.
    Muñoz-Chápuli R
    The Histochemical journal 31.12 (1999 Dec): 771-9.

    Differentiation of the smooth muscle cell phenotypes during embryonic development of coronary vessels in the rat.
    Krämer J
    Histochemistry and cell biology 116.1 (2001 Jul): 79-87.

    Proliferation and differentiation of smooth muscle cell precursors occurs simultaneously during the development of the vessel wall.
    Iruela-Arispe ML
    Developmental dynamics : an official publication of the American Association of Anatomists 209.4 (1997 Aug): 342-52.

    Molecular cloning, functional expression, and tissue distribution of a novel human gap junction-forming protein, connexin-31.9. Interaction with zona occludens protein-1.
    Kumar NM
    The Journal of biological chemistry 277.41 (2002 Oct 11): 38272-83.

    CRISPR/Cas9-based Pten knock-out and Sleeping Beauty Transposon-mediated Nras knock-in induces hepatocellular carcinoma and hepatic lipid accumulation in mice.
    Liu D
    Cancer biology and therapy 18.7 (2017 Jul 3): 505-512.

    Atrial natriuretic peptide inhibits cell cycle activity of embryonic cardiac progenitor cells via its NPRA receptor signaling axis.
    Pasumarthi KB
    American journal of physiology. Cell physiology 308.7 (2015 Apr 1): C557-69.

    Tissue-specific role of the Na,K-ATPase α2 isozyme in skeletal muscle.
    Heiny JA
    The Journal of biological chemistry 288.2 (2013 Jan 11): 1226-37.

    Smooth muscle cell origin and its relation to heterogeneity in development and disease.
    Poelmann RE
    Arteriosclerosis, thrombosis, and vascular biology 19.7 (1999 Jul): 1589-94.

    Comparative study of stromal cell lines derived from embryonic, fetal, and postnatal mouse blood-forming tissues.
    Péault B
    Experimental hematology 30.10 (2002 Oct): 1202-10.

    Vascular smooth muscle differentiation of murine stroma: a sequential model.
    Charbord P
    Experimental hematology 27.12 (1999 Dec): 1782-95.

    Safety concern between autologous fat graft, mesenchymal stem cell and osteosarcoma recurrence.
    Gouin F
    PloS one 5.6 (2010 Jun 8): e10999.

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