1E12
Catalog Fields
Antigen: alpha-actinin, smooth muscle-specific
Hybridoma Cells Available: Yes
Antigen Species: Quail
Depositor: Little, C.D.
Isotype: MIgM
Depositors Institution: University of Kansas Medical Center
Host Species: mouse
Depositors Notes: Before western blotting IgM with 0.5% SDS was used. Amino acid sequence analysis is positive and purification is positive.
Positive Tested Species Reactivity: Canine, Chicken, Dogfish, Human, Monkey, Mouse, Quail, Shark
Gene: ACTN1
Antigen Molecular Weight: 103 kDa
Clonality: Monoclonal
Immunogen: Crude homogenate of embryonic dorsal aorta
Immunogen Sequence: Full length sequence
Uniprot ID: P12814
Additional Information: RRID:AB_2289065
Entrez Gene ID: 87
Antibody Registry ID: AB_2289065
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1E12 was deposited to the DSHB by Little, C.D. (DSHB Hybridoma Product 1E12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
14 References
Initial Publication
Development of the aortic vessel wall as defined by vascular smooth muscle and extracellular matrix markers.
Little CD
Developmental biology 178.2 (1996 Sep 15): 375-92.