1E12
Catalog Fields
Antigen: alpha-actinin, smooth muscle-specific
Hybridoma Cells Available: Yes
Antigen Species: Quail
Depositor: Little, C.D.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Kansas Medical Center
Positive Tested Species Reactivity: Canine, Chicken, Dogfish, Human, Monkey, Mouse, Quail, Shark
Depositors Notes: Before western blotting IgM with 0.5% SDS was used. Amino acid sequence analysis is positive and purification is positive.
Predicted Species Reactivity:
Gene: ACTN1
Immunogen: Crude homogenate of embryonic dorsal aorta
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain:
Epitope Location or Sequence:
Uniprot ID: P12814
Immunogen Sequence: Full length sequence
Entrez Gene ID: 87
Antibody Registry ID: AB_2289065
Additional Information: RRID:AB_2289065
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1E12 was deposited to the DSHB by Little, C.D. (DSHB Hybridoma Product 1E12)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
15 References
Initial Publication
Development of the aortic vessel wall as defined by vascular smooth muscle and extracellular matrix markers.
Little CD
Developmental biology 178.2 (1996 Sep 15): 375-92.