mAb 35

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$40.00
SKU: mAb 35
View product citations for antibody mAb 35 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Acetylcholine receptor nicotinic alpha 1 subunit
Hybridoma Cells Available: Yes
Antigen Species: Electric eel
Depositor: Lindstrom, J.
Isotype: RIgG1
Antigen Sequence:
Host Species: rat
Depositors Institution: Univ of Pennsylvania School of Medicine
Positive Tested Species Reactivity: Bovine, Chicken, Electric eel, Fish, Human, Mouse, Rana Pipiens, Rat, Torpedo, Zebrafish
Depositors Notes: This antibody recognizes muscle nicotinic receptors of most species and binds on the extracellular MIR domain of alpha subunits. mAb 35 also recognizes neuronal nicotinic receptors in birds. No cross-reactivity in Xenopus. mAb 35 has low affinity for denatured receptors and is not recommended for western blots.
Antigen Molecular Weight: 2.8 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: chrna1
Immunogen: Purified receptor
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: S194 15.XX0.BU.1
Epitope Location or Sequence: AChR alpha subunit MIR
Uniprot ID: P09688 
Immunogen Sequence: Full length protein
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_528405 
Additional Information: RRID:AB_528405
Recommended Applications: ELISA, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
mAb 35 was deposited to the DSHB by Lindstrom, J. (DSHB Hybridoma Product mAb 35)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

26 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication

    The Journal of biological chemistry
    Lindstrom JM
    The Journal of biological chemistry 256.16 (1981 Aug 25): 8635-45.

    IF References

    Cell and tissue research
    Shimada Y
    Cell and tissue research 293.2 (1998 Aug): 305-11.

    PLoS genetics
    Fahrenkrog B
    PLoS genetics 14.12 (2018 Dec): e1007845.

    PLoS genetics
    Fahrenkrog B
    PLoS genetics 14.12 (2018 Dec): e1007845.

    PloS one
    Rafuse VF
    PloS one 9.3 (2014): e91643.

    Development (Cambridge, England)
    Mei L
    Development (Cambridge, England) 139.13 (2012 Jul): 2392-404.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Akaaboune M
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.15 (2012 Apr 11): 5177-85.

    The Journal of cell biology
    Brenner HR
    The Journal of cell biology 195.7 (2011 Dec 26): 1171-84.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Landmesser LT
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.3 (2011 Jan 19): 1093-105.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Jacob MH
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.33 (2010 Aug 18): 11073-85.

    Proceedings of the National Academy of Sciences of the United States of America
    Burden SJ
    Proceedings of the National Academy of Sciences of the United States of America 103.16 (2006 Apr 18): 6374-9.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Jacob MH
    The Journal of neuroscience : the official journal of the Society for Neuroscience 24.30 (2004 Jul 28): 6776-84.

    WB References

    The Journal of clinical investigation
    Mei L
    The Journal of clinical investigation 123.12 (2013 Dec): 5190-202.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Lindstrom JM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 7.12 (1987 Dec): 4005-16.

    IHC References

    The Journal of comparative neurology
    Podleski TR
    The Journal of comparative neurology 274.2 (1988 Aug 8): 255-64.

    Journal of neurophysiology
    Markham MR
    Journal of neurophysiology 114.1 (2015 Jul): 520-30.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Rafuse VF
    The Journal of neuroscience : the official journal of the Society for Neuroscience 35.3 (2015 Jan 21): 1291-306.

    Brain research
    Jacob MH
    Brain research 1218. (2008 Jul 7): 21-34.

    The Journal of cell biology
    Barrantes FJ
    The Journal of cell biology 181.7 (2008 Jun 30): 1179-93.

    Molecular and cellular neurosciences
    Jacob MH
    Molecular and cellular neurosciences 38.2 (2008 Jun): 138-52.

    Development (Cambridge, England)
    Akaaboune M
    Development (Cambridge, England) 133.22 (2006 Nov): 4485-93.

    Journal of neurophysiology
    Kuwada JY
    Journal of neurophysiology 92.5 (2004 Nov): 2898-908.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Landmesser LT
    The Journal of neuroscience : the official journal of the Society for Neuroscience 24.8 (2004 Feb 25): 1852-64.

    Cell and tissue research
    Shimada Y
    Cell and tissue research 293.2 (1998 Aug): 305-11.

    Trends in neurosciences
    Margiotta JF
    Trends in neurosciences 12.1 (1989 Jan): 16-21.

    The Journal of comparative neurology
    Podleski TR
    The Journal of comparative neurology 274.2 (1988 Aug 8): 255-64.

    The Journal of cell biology
    Unwin N
    The Journal of cell biology 105.1 (1987 Jul): 9-18.

    Proceedings of the National Academy of Sciences of the United States of America
    Lindstrom JM
    Proceedings of the National Academy of Sciences of the United States of America 79.1 (1982 Jan): 188-92.

    ELISA References

    The Journal of biological chemistry
    Lindstrom JM
    The Journal of biological chemistry 256.16 (1981 Aug 25): 8635-45.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Berg DK
    The Journal of neuroscience : the official journal of the Society for Neuroscience 6.4 (1986 Apr): 946-53.

    Epitope Map References

    Proceedings of the National Academy of Sciences of the United States of America
    Lindstrom JM
    Proceedings of the National Academy of Sciences of the United States of America 79.1 (1982 Jan): 188-92.

    All References

    The Journal of comparative neurology
    Podleski TR
    The Journal of comparative neurology 274.2 (1988 Aug 8): 255-64.

    Journal of neurophysiology
    Markham MR
    Journal of neurophysiology 114.1 (2015 Jul): 520-30.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Rafuse VF
    The Journal of neuroscience : the official journal of the Society for Neuroscience 35.3 (2015 Jan 21): 1291-306.

    Brain research
    Jacob MH
    Brain research 1218. (2008 Jul 7): 21-34.

    The Journal of cell biology
    Barrantes FJ
    The Journal of cell biology 181.7 (2008 Jun 30): 1179-93.

    Molecular and cellular neurosciences
    Jacob MH
    Molecular and cellular neurosciences 38.2 (2008 Jun): 138-52.

    Development (Cambridge, England)
    Akaaboune M
    Development (Cambridge, England) 133.22 (2006 Nov): 4485-93.

    Journal of neurophysiology
    Kuwada JY
    Journal of neurophysiology 92.5 (2004 Nov): 2898-908.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Landmesser LT
    The Journal of neuroscience : the official journal of the Society for Neuroscience 24.8 (2004 Feb 25): 1852-64.

    Cell and tissue research
    Shimada Y
    Cell and tissue research 293.2 (1998 Aug): 305-11.

    Trends in neurosciences
    Margiotta JF
    Trends in neurosciences 12.1 (1989 Jan): 16-21.

    The Journal of cell biology
    Unwin N
    The Journal of cell biology 105.1 (1987 Jul): 9-18.

    Proceedings of the National Academy of Sciences of the United States of America
    Lindstrom JM
    Proceedings of the National Academy of Sciences of the United States of America 79.1 (1982 Jan): 188-92.

    PLoS genetics
    Fahrenkrog B
    PLoS genetics 14.12 (2018 Dec): e1007845.

    PloS one
    Rafuse VF
    PloS one 9.3 (2014): e91643.

    Development (Cambridge, England)
    Mei L
    Development (Cambridge, England) 139.13 (2012 Jul): 2392-404.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Akaaboune M
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.15 (2012 Apr 11): 5177-85.

    The Journal of cell biology
    Brenner HR
    The Journal of cell biology 195.7 (2011 Dec 26): 1171-84.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Landmesser LT
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.3 (2011 Jan 19): 1093-105.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Jacob MH
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.33 (2010 Aug 18): 11073-85.

    Proceedings of the National Academy of Sciences of the United States of America
    Burden SJ
    Proceedings of the National Academy of Sciences of the United States of America 103.16 (2006 Apr 18): 6374-9.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Jacob MH
    The Journal of neuroscience : the official journal of the Society for Neuroscience 24.30 (2004 Jul 28): 6776-84.

    The Journal of clinical investigation
    Mei L
    The Journal of clinical investigation 123.12 (2013 Dec): 5190-202.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Lindstrom JM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 7.12 (1987 Dec): 4005-16.

    The Journal of biological chemistry
    Lindstrom JM
    The Journal of biological chemistry 256.16 (1981 Aug 25): 8635-45.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Berg DK
    The Journal of neuroscience : the official journal of the Society for Neuroscience 6.4 (1986 Apr): 946-53.

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