mAb 35
Catalog Fields
Antigen: Acetylcholine receptor nicotinic alpha 1 subunit
Available to For-Profits: Yes
Antigen Species: Electric eel
Hybridoma Cells Available (Non-Profit): Yes
Isotype: RIgG1
Depositor: Lindstrom, J.
Host Species: rat
Antigen Sequence:
Positive Tested Species Reactivity: Bovine, Chicken, Electric eel, Fish, Human, Mouse, Rana Pipiens, Rat, Torpedo, Zebrafish
Depositors Institution: University of Pennsylvania School of Medicine
Antigen Molecular Weight: 2.8 kDa
Depositors Notes: This antibody recognizes muscle nicotinic receptors of most species and binds on the extracellular MIR domain of alpha subunits. mAb 35 also recognizes neuronal nicotinic receptors in birds. No cross-reactivity in Xenopus. mAb 35 has low affinity for denatured receptors and is not recommended for western blots.
Immunogen: Purified receptor
Gene: chrna1
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: S194 15.XX0.BU.1
Epitope Mapped: Yes
Uniprot ID: P09688
Epitope Location or Sequence: AChR alpha subunit MIR
Antibody Registry ID: AB_528405
Recommended Applications: ELISA, Immunohistochemistry, Immunoprecipitation
Additional Information: RRID:AB_528405
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
mAb 35 was deposited to the DSHB by Lindstrom, J. (DSHB Hybridoma Product mAb 35)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
26 References
Initial Publication
Mapping of surface structures of electrophorus acetylcholine receptor using monoclonal antibodies.
Lindstrom JM
The Journal of biological chemistry 256.16 (1981 Aug 25): 8635-45.