mAb 35

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$40.00
SKU: mAb 35
View product citations for antibody mAb 35 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Acetylcholine receptor nicotinic alpha 1 subunit
Hybridoma Cells Available: Yes
Antigen Species: Electric eel
Depositor: Lindstrom, J.
Isotype: RIgG1
Antigen Sequence:
Host Species: rat
Depositors Institution: Univ of Pennsylvania School of Medicine
Positive Tested Species Reactivity: Bovine, Chicken, Electric eel, Fish, Human, Mouse, Rana Pipiens, Rat, Torpedo, Zebrafish
Depositors Notes: This antibody recognizes muscle nicotinic receptors of most species and binds on the extracellular MIR domain of alpha subunits. mAb 35 also recognizes neuronal nicotinic receptors in birds. No cross-reactivity in Xenopus. mAb 35 has low affinity for denatured receptors and is not recommended for western blots.
Antigen Molecular Weight: 2.8 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: chrna1
Immunogen: Purified receptor
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: S194 15.XX0.BU.1
Epitope Location or Sequence: AChR alpha subunit MIR
Uniprot ID: P09688 
Immunogen Sequence: Full length protein
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_528405 
Additional Information: RRID:AB_528405
Recommended Applications: ELISA, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
mAb 35 was deposited to the DSHB by Lindstrom, J. (DSHB Hybridoma Product mAb 35)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

26 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication

    Mapping of surface structures of electrophorus acetylcholine receptor using monoclonal antibodies.
    Lindstrom JM
    The Journal of biological chemistry 256.16 (1981 Aug 25): 8635-45.

    IF References

    Differentiation of muscle-specific proteins in chicken somites as studied by immunofluorescence microscopy.
    Shimada Y
    Cell and tissue research 293.2 (1998 Aug): 305-11.

    Biallelic mutations in nucleoporin NUP88 cause lethal fetal akinesia deformation sequence.
    Fahrenkrog B
    PLoS genetics 14.12 (2018 Dec): e1007845.

    Biallelic mutations in nucleoporin NUP88 cause lethal fetal akinesia deformation sequence.
    Fahrenkrog B
    PLoS genetics 14.12 (2018 Dec): e1007845.

    A stem-cell based bioassay to critically assess the pathology of dysfunctional neuromuscular junctions.
    Rafuse VF
    PloS one 9.3 (2014): e91643.

    β-Catenin gain of function in muscles impairs neuromuscular junction formation.
    Mei L
    Development (Cambridge, England) 139.13 (2012 Jul): 2392-404.

    A role for the calmodulin kinase II-related anchoring protein (αkap) in maintaining the stability of nicotinic acetylcholine receptors.
    Akaaboune M
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.15 (2012 Apr 11): 5177-85.

    Neuregulin/ErbB regulate neuromuscular junction development by phosphorylation of α-dystrobrevin.
    Brenner HR
    The Journal of cell biology 195.7 (2011 Dec 26): 1171-84.

    Frequency-dependent modes of synaptic vesicle endocytosis and exocytosis at adult mouse neuromuscular junctions.
    Landmesser LT
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.3 (2011 Jan 19): 1093-105.

    The postsynaptic adenomatous polyposis coli (APC) multiprotein complex is required for localizing neuroligin and neurexin to neuronal nicotinic synapses in vivo.
    Jacob MH
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.33 (2010 Aug 18): 11073-85.

    CD24 is expressed by myofiber synaptic nuclei and regulates synaptic transmission.
    Burden SJ
    Proceedings of the National Academy of Sciences of the United States of America 103.16 (2006 Apr 18): 6374-9.

    Neuronal nicotinic synapse assembly requires the adenomatous polyposis coli tumor suppressor protein.
    Jacob MH
    The Journal of neuroscience : the official journal of the Society for Neuroscience 24.30 (2004 Jul 28): 6776-84.

    WB References

    Antibodies against low-density lipoprotein receptor-related protein 4 induce myasthenia gravis.
    Mei L
    The Journal of clinical investigation 123.12 (2013 Dec): 5190-202.

    Structurally different neuronal nicotinic acetylcholine receptor subtypes purified and characterized using monoclonal antibodies.
    Lindstrom JM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 7.12 (1987 Dec): 4005-16.

    IHC References

    Autoradiographic localization of nicotinic acetylcholine receptors in the brain of the zebra finch (Poephila guttata).
    Podleski TR
    The Journal of comparative neurology 274.2 (1988 Aug 8): 255-64.

    A highly polarized excitable cell separates sodium channels from sodium-activated potassium channels by more than a millimeter.
    Markham MR
    Journal of neurophysiology 114.1 (2015 Jul): 520-30.

    Motoneurons derived from induced pluripotent stem cells develop mature phenotypes typical of endogenous spinal motoneurons.
    Rafuse VF
    The Journal of neuroscience : the official journal of the Society for Neuroscience 35.3 (2015 Jan 21): 1291-306.

    Dystrophin and utrophin isoforms are expressed in glia, but not neurons, of the avian parasympathetic ciliary ganglion.
    Jacob MH
    Brain research 1218. (2008 Jul 7): 21-34.

    Nicotinic acetylcholine receptor is internalized via a Rac-dependent, dynamin-independent endocytic pathway.
    Barrantes FJ
    The Journal of cell biology 181.7 (2008 Jun 30): 1179-93.

    Adenomatous polyposis coli plays a key role, in vivo, in coordinating assembly of the neuronal nicotinic postsynaptic complex.
    Jacob MH
    Molecular and cellular neurosciences 38.2 (2008 Jun): 138-52.

    The dynamics of recycled acetylcholine receptors at the neuromuscular junction in vivo.
    Akaaboune M
    Development (Cambridge, England) 133.22 (2006 Nov): 4485-93.

    shocked Gene is required for the function of a premotor network in the zebrafish CNS.
    Kuwada JY
    Journal of neurophysiology 92.5 (2004 Nov): 2898-908.

    Distinct roles of different neural cell adhesion molecule (NCAM) isoforms in synaptic maturation revealed by analysis of NCAM 180 kDa isoform-deficient mice.
    Landmesser LT
    The Journal of neuroscience : the official journal of the Society for Neuroscience 24.8 (2004 Feb 25): 1852-64.

    Differentiation of muscle-specific proteins in chicken somites as studied by immunofluorescence microscopy.
    Shimada Y
    Cell and tissue research 293.2 (1998 Aug): 305-11.

    Regulating the number and function of neuronal acetylcholine receptors.
    Margiotta JF
    Trends in neurosciences 12.1 (1989 Jan): 16-21.

    Autoradiographic localization of nicotinic acetylcholine receptors in the brain of the zebra finch (Poephila guttata).
    Podleski TR
    The Journal of comparative neurology 274.2 (1988 Aug 8): 255-64.

    Location of subunits within the acetylcholine receptor by electron image analysis of tubular crystals from Torpedo marmorata.
    Unwin N
    The Journal of cell biology 105.1 (1987 Jul): 9-18.

    Specificities of antibodies to acetylcholine receptors in sera from myasthenia gravis patients measured by monoclonal antibodies.
    Lindstrom JM
    Proceedings of the National Academy of Sciences of the United States of America 79.1 (1982 Jan): 188-92.

    ELISA References

    Mapping of surface structures of electrophorus acetylcholine receptor using monoclonal antibodies.
    Lindstrom JM
    The Journal of biological chemistry 256.16 (1981 Aug 25): 8635-45.

    Cholinergic modulation of an acetylcholine receptor-like antigen on the surface of chick ciliary ganglion neurons in cell culture.
    Berg DK
    The Journal of neuroscience : the official journal of the Society for Neuroscience 6.4 (1986 Apr): 946-53.

    Epitope Map References

    Specificities of antibodies to acetylcholine receptors in sera from myasthenia gravis patients measured by monoclonal antibodies.
    Lindstrom JM
    Proceedings of the National Academy of Sciences of the United States of America 79.1 (1982 Jan): 188-92.

    All References

    Autoradiographic localization of nicotinic acetylcholine receptors in the brain of the zebra finch (Poephila guttata).
    Podleski TR
    The Journal of comparative neurology 274.2 (1988 Aug 8): 255-64.

    A highly polarized excitable cell separates sodium channels from sodium-activated potassium channels by more than a millimeter.
    Markham MR
    Journal of neurophysiology 114.1 (2015 Jul): 520-30.

    Motoneurons derived from induced pluripotent stem cells develop mature phenotypes typical of endogenous spinal motoneurons.
    Rafuse VF
    The Journal of neuroscience : the official journal of the Society for Neuroscience 35.3 (2015 Jan 21): 1291-306.

    Dystrophin and utrophin isoforms are expressed in glia, but not neurons, of the avian parasympathetic ciliary ganglion.
    Jacob MH
    Brain research 1218. (2008 Jul 7): 21-34.

    Nicotinic acetylcholine receptor is internalized via a Rac-dependent, dynamin-independent endocytic pathway.
    Barrantes FJ
    The Journal of cell biology 181.7 (2008 Jun 30): 1179-93.

    Adenomatous polyposis coli plays a key role, in vivo, in coordinating assembly of the neuronal nicotinic postsynaptic complex.
    Jacob MH
    Molecular and cellular neurosciences 38.2 (2008 Jun): 138-52.

    The dynamics of recycled acetylcholine receptors at the neuromuscular junction in vivo.
    Akaaboune M
    Development (Cambridge, England) 133.22 (2006 Nov): 4485-93.

    shocked Gene is required for the function of a premotor network in the zebrafish CNS.
    Kuwada JY
    Journal of neurophysiology 92.5 (2004 Nov): 2898-908.

    Distinct roles of different neural cell adhesion molecule (NCAM) isoforms in synaptic maturation revealed by analysis of NCAM 180 kDa isoform-deficient mice.
    Landmesser LT
    The Journal of neuroscience : the official journal of the Society for Neuroscience 24.8 (2004 Feb 25): 1852-64.

    Differentiation of muscle-specific proteins in chicken somites as studied by immunofluorescence microscopy.
    Shimada Y
    Cell and tissue research 293.2 (1998 Aug): 305-11.

    Regulating the number and function of neuronal acetylcholine receptors.
    Margiotta JF
    Trends in neurosciences 12.1 (1989 Jan): 16-21.

    Location of subunits within the acetylcholine receptor by electron image analysis of tubular crystals from Torpedo marmorata.
    Unwin N
    The Journal of cell biology 105.1 (1987 Jul): 9-18.

    Specificities of antibodies to acetylcholine receptors in sera from myasthenia gravis patients measured by monoclonal antibodies.
    Lindstrom JM
    Proceedings of the National Academy of Sciences of the United States of America 79.1 (1982 Jan): 188-92.

    Biallelic mutations in nucleoporin NUP88 cause lethal fetal akinesia deformation sequence.
    Fahrenkrog B
    PLoS genetics 14.12 (2018 Dec): e1007845.

    A stem-cell based bioassay to critically assess the pathology of dysfunctional neuromuscular junctions.
    Rafuse VF
    PloS one 9.3 (2014): e91643.

    β-Catenin gain of function in muscles impairs neuromuscular junction formation.
    Mei L
    Development (Cambridge, England) 139.13 (2012 Jul): 2392-404.

    A role for the calmodulin kinase II-related anchoring protein (αkap) in maintaining the stability of nicotinic acetylcholine receptors.
    Akaaboune M
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.15 (2012 Apr 11): 5177-85.

    Neuregulin/ErbB regulate neuromuscular junction development by phosphorylation of α-dystrobrevin.
    Brenner HR
    The Journal of cell biology 195.7 (2011 Dec 26): 1171-84.

    Frequency-dependent modes of synaptic vesicle endocytosis and exocytosis at adult mouse neuromuscular junctions.
    Landmesser LT
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.3 (2011 Jan 19): 1093-105.

    The postsynaptic adenomatous polyposis coli (APC) multiprotein complex is required for localizing neuroligin and neurexin to neuronal nicotinic synapses in vivo.
    Jacob MH
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.33 (2010 Aug 18): 11073-85.

    CD24 is expressed by myofiber synaptic nuclei and regulates synaptic transmission.
    Burden SJ
    Proceedings of the National Academy of Sciences of the United States of America 103.16 (2006 Apr 18): 6374-9.

    Neuronal nicotinic synapse assembly requires the adenomatous polyposis coli tumor suppressor protein.
    Jacob MH
    The Journal of neuroscience : the official journal of the Society for Neuroscience 24.30 (2004 Jul 28): 6776-84.

    Antibodies against low-density lipoprotein receptor-related protein 4 induce myasthenia gravis.
    Mei L
    The Journal of clinical investigation 123.12 (2013 Dec): 5190-202.

    Structurally different neuronal nicotinic acetylcholine receptor subtypes purified and characterized using monoclonal antibodies.
    Lindstrom JM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 7.12 (1987 Dec): 4005-16.

    Mapping of surface structures of electrophorus acetylcholine receptor using monoclonal antibodies.
    Lindstrom JM
    The Journal of biological chemistry 256.16 (1981 Aug 25): 8635-45.

    Cholinergic modulation of an acetylcholine receptor-like antigen on the surface of chick ciliary ganglion neurons in cell culture.
    Berg DK
    The Journal of neuroscience : the official journal of the Society for Neuroscience 6.4 (1986 Apr): 946-53.

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