mAB 270

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$40.00
SKU: mAB 270
View product citations for antibody mAB 270 on CiteAb

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Available: 75

DSHB Data Sheet

Catalog Fields

Antigen: Neuronal nicotinic acetylcholine receptor subunit beta-2
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Lindstrom, J.
Isotype: RIgG2a
Antigen Sequence:
Host Species: rat
Depositors Institution: Univ of Pennsylvania School of Medicine
Positive Tested Species Reactivity: Chicken, Guinea Pig, Mouse, Rat
Depositors Notes: mAB 270 does not recognize either the human or bovine nicotinic receptor. mAB 270 does recognize cation channels in PC12 cells but does not bind to muscle AChRs.
Antigen Molecular Weight: 56 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: CHRNB2
Immunogen: mAB 35 affinity purified AChR
Alternate Gene Names: NACHRB2
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: S194 15.XX0.BU.1
Epitope Location or Sequence: AChR alpha subunit
Uniprot ID: P09484 
Immunogen Sequence:
Entrez Gene ID: 395605 
Additional Characterization:  
Antibody Registry ID: AB_531809 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
mAB 270 was deposited to the DSHB by Lindstrom, J. (DSHB Hybridoma Product mAB 270)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

11 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Nicotine-induced upregulation of native neuronal nicotinic receptors is caused by multiple mechanisms.
    Green WN
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.6 (2012 Feb 8): 2227-38.

    Somatic tetraploidy in specific chick retinal ganglion cells induced by nerve growth factor.
    Frade JM
    Proceedings of the National Academy of Sciences of the United States of America 107.1 (2010 Jan 5): 109-14.

    Ubiquilin-1 regulates nicotine-induced up-regulation of neuronal nicotinic acetylcholine receptors.
    Feng G
    The Journal of biological chemistry 280.40 (2005 Oct 7): 34088-95.

    PSD93 regulates synaptic stability at neuronal cholinergic synapses.
    Feng G
    The Journal of neuroscience : the official journal of the Society for Neuroscience 24.2 (2004 Jan 14): 378-88.

    Cholinoceptive neurons in the retina of the chick: an immunohistochemical study of the nicotinic acetylcholine receptors.
    Karten HJ
    Visual neuroscience 1.4 (1988): 349-66.

    WB References

    Pharmacological properties of immuno-isolated neuronal nicotinic receptors.
    Lindstrom J
    The Journal of neuroscience : the official journal of the Society for Neuroscience 6.10 (1986 Oct): 3061-9.

    Structurally different neuronal nicotinic acetylcholine receptor subtypes purified and characterized using monoclonal antibodies.
    Lindstrom JM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 7.12 (1987 Dec): 4005-16.

    IHC References

    Somatic tetraploidy in specific chick retinal ganglion cells induced by nerve growth factor.
    Frade JM
    Proceedings of the National Academy of Sciences of the United States of America 107.1 (2010 Jan 5): 109-14.

    Pharmacological properties of nicotinic acetylcholine receptors expressed by guinea pig small intestinal myenteric neurons.
    Galligan JJ
    The Journal of pharmacology and experimental therapeutics 302.3 (2002 Sep): 889-97.

    Immunohistochemical localization of neuronal nicotinic receptors in the rodent central nervous system.
    Lindstrom J
    The Journal of neuroscience : the official journal of the Society for Neuroscience 7.10 (1987 Oct): 3334-42.

    IP References

    Nicotine-induced upregulation of native neuronal nicotinic receptors is caused by multiple mechanisms.
    Green WN
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.6 (2012 Feb 8): 2227-38.

    Structurally different neuronal nicotinic acetylcholine receptor subtypes purified and characterized using monoclonal antibodies.
    Lindstrom JM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 7.12 (1987 Dec): 4005-16.

    Epitope Map References

    Purification and characterization of a nicotinic acetylcholine receptor from rat brain.
    Lindstrom J
    Proceedings of the National Academy of Sciences of the United States of America 84.2 (1987 Jan): 595-9.

    All References

    Somatic tetraploidy in specific chick retinal ganglion cells induced by nerve growth factor.
    Frade JM
    Proceedings of the National Academy of Sciences of the United States of America 107.1 (2010 Jan 5): 109-14.

    Pharmacological properties of nicotinic acetylcholine receptors expressed by guinea pig small intestinal myenteric neurons.
    Galligan JJ
    The Journal of pharmacology and experimental therapeutics 302.3 (2002 Sep): 889-97.

    Immunohistochemical localization of neuronal nicotinic receptors in the rodent central nervous system.
    Lindstrom J
    The Journal of neuroscience : the official journal of the Society for Neuroscience 7.10 (1987 Oct): 3334-42.

    Nicotine-induced upregulation of native neuronal nicotinic receptors is caused by multiple mechanisms.
    Green WN
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.6 (2012 Feb 8): 2227-38.

    Ubiquilin-1 regulates nicotine-induced up-regulation of neuronal nicotinic acetylcholine receptors.
    Feng G
    The Journal of biological chemistry 280.40 (2005 Oct 7): 34088-95.

    PSD93 regulates synaptic stability at neuronal cholinergic synapses.
    Feng G
    The Journal of neuroscience : the official journal of the Society for Neuroscience 24.2 (2004 Jan 14): 378-88.

    Cholinoceptive neurons in the retina of the chick: an immunohistochemical study of the nicotinic acetylcholine receptors.
    Karten HJ
    Visual neuroscience 1.4 (1988): 349-66.

    Pharmacological properties of immuno-isolated neuronal nicotinic receptors.
    Lindstrom J
    The Journal of neuroscience : the official journal of the Society for Neuroscience 6.10 (1986 Oct): 3061-9.

    Structurally different neuronal nicotinic acetylcholine receptor subtypes purified and characterized using monoclonal antibodies.
    Lindstrom JM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 7.12 (1987 Dec): 4005-16.

    Purification and characterization of a nicotinic acetylcholine receptor from chick brain.
    Lindstrom JM
    Biochemistry 25.8 (1986 Apr 22): 2082-93.

    Purification and characterization of a nicotinic acetylcholine receptor from rat brain.
    Lindstrom J
    Proceedings of the National Academy of Sciences of the United States of America 84.2 (1987 Jan): 595-9.

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