mAB 270
Catalog Fields
Clone ID/Product Name: mAB 270
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: CHRNB2
Ab Isotype: RIgG2a
Gene Name:
Entrez Gene ID: 395605
Clonality: Monoclonal
Immunogen: mAB 35 affinity purified AChR
Clone:
Immunogen Sequence:
Myeloma Strain: S194 15.XX0.BU.1
Epitope Mapped: Yes
Antigen Name: Neuronal nicotinic acetylcholine receptor subunit beta-2
Epitope Location or Sequence: AChR alpha subunit
Alternate Antigen Name:
Deposit Date: 2/7/1989
Antigen Molecular Weight: 56 kDa
Depositor: Lindstrom, J.
Antigen Sequence:
Depositor Institution: University of Pennsylvania School of Medicine
Antigen Species: Chicken
Depositor Notes: mAB 270 does not recognize either the human or bovine nicotinic receptor. mAB 270 does recognize cation channels in PC12 cells but does not bind to muscle AChRs.
Host Species: rat
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Chicken, Guinea Pig, Mouse, Rat
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
mAB 270 was deposited to the DSHB by Lindstrom, J. (DSHB Hybridoma Product mAB 270)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
11 References
Initial Publication
Purification and characterization of a nicotinic acetylcholine receptor from chick brain.
Lindstrom JM
Biochemistry 25.8 (1986 Apr 22): 2082-93.