mAB 270

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$45.00 to $425.00
SKU: mAB 270
View product citations for antibody mAB 270 on CiteAb

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DSHB Data Sheet

Catalog Fields

Product Name/ID: mAB 270
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: CHRNB2
Ab Isotype: RIgG2a
Gene Symbol:
Antibody Registry ID: AB_531809 
Uniprot ID: P09484 
RRID:  
Entrez Gene ID: 395605 
Clonality: Monoclonal
Immunogen: mAB 35 affinity purified AChR
Clone:
Immunogen Sequence:
Myeloma Strain: S194 15.XX0.BU.1
Epitope Mapped: Yes
Antigen Name: Neuronal nicotinic acetylcholine receptor subunit beta-2
Epitope Location or Sequence: AChR alpha subunit
Alternate Antigen Name:
Deposit Date: 2/7/1989
Antigen Molecular Weight: 56 kDa
Depositor: Lindstrom, J.
Antigen Sequence:
Depositor Institution: University of Pennsylvania School of Medicine
Antigen Species: Chicken
Depositor Notes: mAB 270 does not recognize either the human or bovine nicotinic receptor. mAB 270 does recognize cation channels in PC12 cells but does not bind to muscle AChRs.
Host Species: rat
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Chicken, Guinea Pig, Mouse, Rat
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
mAB 270 was deposited to the DSHB by Lindstrom, J. (DSHB Hybridoma Product mAB 270)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

11 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Nicotine-induced upregulation of native neuronal nicotinic receptors is caused by multiple mechanisms.
    Green WN
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.6 (2012 Feb 8): 2227-38.

    Somatic tetraploidy in specific chick retinal ganglion cells induced by nerve growth factor.
    Frade JM
    Proceedings of the National Academy of Sciences of the United States of America 107.1 (2010 Jan 5): 109-14.

    Ubiquilin-1 regulates nicotine-induced up-regulation of neuronal nicotinic acetylcholine receptors.
    Feng G
    The Journal of biological chemistry 280.40 (2005 Oct 7): 34088-95.

    PSD93 regulates synaptic stability at neuronal cholinergic synapses.
    Feng G
    The Journal of neuroscience : the official journal of the Society for Neuroscience 24.2 (2004 Jan 14): 378-88.

    Cholinoceptive neurons in the retina of the chick: an immunohistochemical study of the nicotinic acetylcholine receptors.
    Karten HJ
    Visual neuroscience 1.4 (1988): 349-66.

    WB References

    Pharmacological properties of immuno-isolated neuronal nicotinic receptors.
    Lindstrom J
    The Journal of neuroscience : the official journal of the Society for Neuroscience 6.10 (1986 Oct): 3061-9.

    Structurally different neuronal nicotinic acetylcholine receptor subtypes purified and characterized using monoclonal antibodies.
    Lindstrom JM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 7.12 (1987 Dec): 4005-16.

    IHC References

    Somatic tetraploidy in specific chick retinal ganglion cells induced by nerve growth factor.
    Frade JM
    Proceedings of the National Academy of Sciences of the United States of America 107.1 (2010 Jan 5): 109-14.

    Pharmacological properties of nicotinic acetylcholine receptors expressed by guinea pig small intestinal myenteric neurons.
    Galligan JJ
    The Journal of pharmacology and experimental therapeutics 302.3 (2002 Sep): 889-97.

    Immunohistochemical localization of neuronal nicotinic receptors in the rodent central nervous system.
    Lindstrom J
    The Journal of neuroscience : the official journal of the Society for Neuroscience 7.10 (1987 Oct): 3334-42.

    IP References

    Nicotine-induced upregulation of native neuronal nicotinic receptors is caused by multiple mechanisms.
    Green WN
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.6 (2012 Feb 8): 2227-38.

    Structurally different neuronal nicotinic acetylcholine receptor subtypes purified and characterized using monoclonal antibodies.
    Lindstrom JM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 7.12 (1987 Dec): 4005-16.

    Epitope Map References

    Purification and characterization of a nicotinic acetylcholine receptor from rat brain.
    Lindstrom J
    Proceedings of the National Academy of Sciences of the United States of America 84.2 (1987 Jan): 595-9.

    All References

    Somatic tetraploidy in specific chick retinal ganglion cells induced by nerve growth factor.
    Frade JM
    Proceedings of the National Academy of Sciences of the United States of America 107.1 (2010 Jan 5): 109-14.

    Pharmacological properties of nicotinic acetylcholine receptors expressed by guinea pig small intestinal myenteric neurons.
    Galligan JJ
    The Journal of pharmacology and experimental therapeutics 302.3 (2002 Sep): 889-97.

    Immunohistochemical localization of neuronal nicotinic receptors in the rodent central nervous system.
    Lindstrom J
    The Journal of neuroscience : the official journal of the Society for Neuroscience 7.10 (1987 Oct): 3334-42.

    Nicotine-induced upregulation of native neuronal nicotinic receptors is caused by multiple mechanisms.
    Green WN
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.6 (2012 Feb 8): 2227-38.

    Ubiquilin-1 regulates nicotine-induced up-regulation of neuronal nicotinic acetylcholine receptors.
    Feng G
    The Journal of biological chemistry 280.40 (2005 Oct 7): 34088-95.

    PSD93 regulates synaptic stability at neuronal cholinergic synapses.
    Feng G
    The Journal of neuroscience : the official journal of the Society for Neuroscience 24.2 (2004 Jan 14): 378-88.

    Cholinoceptive neurons in the retina of the chick: an immunohistochemical study of the nicotinic acetylcholine receptors.
    Karten HJ
    Visual neuroscience 1.4 (1988): 349-66.

    Pharmacological properties of immuno-isolated neuronal nicotinic receptors.
    Lindstrom J
    The Journal of neuroscience : the official journal of the Society for Neuroscience 6.10 (1986 Oct): 3061-9.

    Structurally different neuronal nicotinic acetylcholine receptor subtypes purified and characterized using monoclonal antibodies.
    Lindstrom JM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 7.12 (1987 Dec): 4005-16.

    Purification and characterization of a nicotinic acetylcholine receptor from chick brain.
    Lindstrom JM
    Biochemistry 25.8 (1986 Apr 22): 2082-93.

    Purification and characterization of a nicotinic acetylcholine receptor from rat brain.
    Lindstrom J
    Proceedings of the National Academy of Sciences of the United States of America 84.2 (1987 Jan): 595-9.

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