151-IgG or 151-8 AE4

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$45.00 to $425.00
SKU: 151-IgG or 151-8 AE4
View product citations for antibody 151-IgG or 151-8 AE4 on CiteAb

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Available: 40

DSHB Data Sheet

Catalog Fields

Product Name/ID: 151-IgG or 151-8 AE4
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name:
Ab Isotype: MIgG2a, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_2230954 
Uniprot ID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: n-octyl glucoside-solubilized protein from isolated PC12 cell plasma membranes, see Chandler et al., J. Biol. Chem. 260, 3360-3367 (1985).
Immunogen Sequence:
Myeloma Strain: Designation of Sp2/0-Ag14
Epitope Mapped: No
Antigen Name: epidermal growth factor receptor
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 3/24/1992
Antigen Molecular Weight: 170-180 kDa
Depositor: Hubbard, A.
Antigen Sequence:
Depositor Institution: Johns Hopkins University
Antigen Species: rat
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Rat
Additional Information: RRID:AB_2230954
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
151-IgG or 151-8 AE4 was deposited to the DSHB by Hubbard, A. (DSHB Hybridoma Product 151-IgG or 151-8 AE4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

5 References

  • Initial Publication
  • All References
  • Initial Publication
    All References

    A monoclonal antibody which inhibits epidermal growth factor binding has opposite effects on the biological action of epidermal growth factor in different cells.
    Shooter EM
    The Journal of biological chemistry 260.6 (1985 Mar 25): 3360-7.

    The role of transforming growth factor alpha in rat craniofacial development and chondrogenesis.
    Sandra A
    Journal of anatomy 189 ( Pt 1).Pt 1 (1996 Aug): 73-86.

    An immunochemical, ultrastructural, and developmental characterization of the horizontal basal cells of rat olfactory epithelium.
    Schwob JE
    The Journal of comparative neurology 363.1 (1995 Dec 4): 129-46.

    Existence of reserve quiescent stem cells in adults, from amphibians to humans.
    Young HE
    Current topics in microbiology and immunology 280. (2004): 71-109.

    Adult stem cells.
    Black AC Jr
    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 276.1 (2004 Jan): 75-102.

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