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$45.00 to $75.00
SKU: 34C
View product citations for antibody 34C on CiteAb

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DSHB Data Sheet

Catalog Fields

Product Name/ID: 34C
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: RYR1/RYR2
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_528457 
Uniprot ID: P21817 
Entrez Gene ID: 6261 
Clonality: Monoclonal
Immunogen: Microsomal membrane preparation from skeletal muscle
Immunogen Sequence: Purified alpha and beta ryanodine receptor
Myeloma Strain: P3x63Ag8.653
Epitope Mapped: Yes
Antigen Name: Ryanodine receptor
Epitope Location or Sequence: a.a. 2756-2803 (RyR1) and a.a. 2722-2769 (RyR2) )
Alternate Antigen Name:
Deposit Date: 2/29/1996
Antigen Molecular Weight: 562.7 kDa
Depositor: Airey, J. / Sutko, J.
Antigen Sequence:
Depositor Institution: University of Nevada
Antigen Species: chicken
Depositor Notes: This antigen is a calcium release channel localized in the sarcoplasmic reticulum.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Amphibian, Avian, Fish, Mammal
Additional Information: RRID:AB_528457
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
34C was deposited to the DSHB by Airey, J. / Sutko, J. (DSHB Hybridoma Product 34C)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

16 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FFPE References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    IP References
    FFPE References
    Epitope Map References
    All References

    Evaluation of adipose-derived stem cells for tissue-engineered muscle repair construct-mediated repair of a murine model of volumetric muscle loss injury.
    Kesireddy V
    International journal of nanomedicine 11. (2016): 1461-73.

    Oxidative stress, mitochondrial damage, and cores in muscle from calsequestrin-1 knockout mice.
    Protasi F
    Skeletal muscle 5. (2015): 10.

    Further development of a tissue engineered muscle repair construct in vitro for enhanced functional recovery following implantation in vivo in a murine model of volumetric muscle loss injury.
    Christ GJ
    Tissue engineering. Part A 18.11-12 (2012 Jun): 1213-28.

    Identification and localization of two triad junctional foot protein isoforms in mature avian fast twitch skeletal muscle.
    Sutko JL
    The Journal of biological chemistry 265.24 (1990 Aug 25): 14187-94.

    Binding properties of the galactose-detecting lectin Pseudomonas aeruginosa agglutinin (PA-IL) to skeletal muscle fibres. Quantitative precipitation and precipitation inhibition assays.
    Høyer PE
    The Histochemical journal 31.7 (1999 Jul): 485-93.

    Identification of a ryanodine receptor in rat heart mitochondria.
    Sheu SS
    The Journal of biological chemistry 276.24 (2001 Jun 15): 21482-8.

    Endogenous calpain-3 activation is primarily governed by small increases in resting cytoplasmic [Ca2+] and is not dependent on stretch.
    Lamb GD
    The Journal of biological chemistry 284.12 (2009 Mar 20): 7811-9.

    Regions of ryanodine receptors that influence activation by the dihydropyridine receptor β1a subunit.
    Dulhunty AF
    Skeletal muscle 5. (2015): 23.

    Triadin binding to the C-terminal luminal loop of the ryanodine receptor is important for skeletal muscle excitation contraction coupling.
    Dirksen RT
    The Journal of general physiology 130.4 (2007 Oct): 365-78.

    Sex-Dependent Effects of 2,2',3,5',6-Pentachlorobiphenyl on Dendritic Arborization of Primary Mouse Neurons.
    Lein PJ
    Toxicological sciences : an official journal of the Society of Toxicology 168.1 (2019 Mar 1): 95-109.

    Enhanced technique to measure proteins in single segments of human skeletal muscle fibers: fiber-type dependence of AMPK-alpha1 and -beta1.
    Murphy RM
    Journal of applied physiology (Bethesda, Md. : 1985) 110.3 (2011 Mar): 820-5.

    Divergent Activity Profiles of Type 1 Ryanodine Receptor Channels Carrying Malignant Hyperthermia and Central Core Disease Mutations in the Amino-Terminal Region.
    Sakurai T
    PloS one 10.6 (2015): e0130606.

    A new cytoplasmic interaction between junctin and ryanodine receptor Ca2+ release channels.
    Dulhunty AF
    Journal of cell science 128.5 (2015 Mar 1): 951-63.

    Caffeine and halothane sensitivity of intracellular Ca2+ release is altered by 15 calcium release channel (ryanodine receptor) mutations associated with malignant hyperthermia and/or central core disease.
    MacLennan DH
    The Journal of biological chemistry 272.42 (1997 Oct 17): 26332-9.

    Three-dimensional localization of serine 2808, a phosphorylation site in cardiac ryanodine receptor.
    Liu Z
    The Journal of biological chemistry 282.35 (2007 Aug 31): 25929-39.

    Localization of the dantrolene-binding sequence near the FK506-binding protein-binding site in the three-dimensional structure of the ryanodine receptor.
    Chen SR
    The Journal of biological chemistry 286.14 (2011 Apr 8): 12202-12.

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