CaF2-5D2
Catalog Fields
Product Name/ID: CaF2-5D2
Available to For-Profits: Yes
Alternate Antibody Name: CAF2; 5D2
Gene Name: ATP2A1
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Entrez Gene ID: 396528
Clonality: Monoclonal
Immunogen: Sarcoplasmic reticulum membrane vesicles preparation from adult chicken pectoralis major muscle.
Clone:
Immunogen Sequence: Total protein
Myeloma Strain: SP2/0 Ag8
Epitope Mapped: No
Antigen Name: ATPase, Ca(+2) fast twitch SR / SERCA1
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 10/10/1990
Antigen Molecular Weight: 110 kDa
Depositor: Fambrough, D.M.
Antigen Sequence:
Depositor Institution: Johns Hopkins School of Medicine
Antigen Species: Chicken
Depositor Notes: Immunoblotting: very good. Immunohistochemistry: good on cryosections and methanol and formaldehyde fixed tissues.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human, Mouse, Rat, Vertebrates
Additional Information:
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CaF2-5D2 was deposited to the DSHB by Fambrough, D.M. (DSHB Hybridoma Product CaF2-5D2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
18 References
Initial Publication
Expression of fast and slow isoforms of the Ca2+-ATPase in developing chick skeletal muscle.
Fambrough DM
Developmental biology 124.2 (1987 Dec): 490-503.