CaF2-5D2

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$40.00
SKU: CaF2-5D2
View product citations for antibody CaF2-5D2 on CiteAb

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Available: 161

DSHB Data Sheet

Catalog Fields

Antigen: ATPase, Ca(+2) fast twitch SR / SERCA1
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Fambrough, D.M.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Johns Hopkins School of Medicine
Positive Tested Species Reactivity: Human, Mouse, Rat, Vertebrates
Depositors Notes: Immunoblotting: very good. Immunohistochemistry: good on cryosections and methanol and formaldehyde fixed tissues.
Antigen Molecular Weight: 110 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: ATP2A1
Immunogen: Sarcoplasmic reticulum membrane vesicles preparation from adult chicken pectoralis major muscle.
Alternate Gene Names: SERCA1
Alternate Antibody Name: CAF2; 5D2
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0 Ag8
Epitope Location or Sequence:
Uniprot ID: P13585 
Immunogen Sequence: Total protein
Entrez Gene ID: 396528 
Additional Characterization:  
Antibody Registry ID: AB_531812 
Additional Information:
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CaF2-5D2 was deposited to the DSHB by Fambrough, D.M. (DSHB Hybridoma Product CaF2-5D2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

18 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • All References
  • Initial Publication
    IF References

    Dysferlin and myoferlin regulate transverse tubule formation and glycerol sensitivity.
    McNally EM
    The American journal of pathology 184.1 (2014 Jan): 248-59.

    The amino-terminal 200 amino acids of the plasma membrane Na+,K+-ATPase alpha subunit confer ouabain sensitivity on the sarcoplasmic reticulum Ca(2+)-ATPase.
    Takeyasu K
    Proceedings of the National Academy of Sciences of the United States of America 90.19 (1993 Oct 1): 8881-5.

    Correct targeting of dihydropyridine receptors and triadin in dyspedic mouse skeletal muscle in vivo.
    Franzini-Armstrong C
    Developmental dynamics : an official publication of the American Association of Anatomists 214.4 (1999 Apr): 372-80.

    WB References

    The effect of taurine and β-alanine supplementation on taurine transporter protein and fatigue resistance in skeletal muscle from mdx mice.
    Goodman CA
    Amino acids 48.11 (2016 Nov): 2635-2645.

    Enhanced technique to measure proteins in single segments of human skeletal muscle fibers: fiber-type dependence of AMPK-alpha1 and -beta1.
    Murphy RM
    Journal of applied physiology (Bethesda, Md. : 1985) 110.3 (2011 Mar): 820-5.

    Calsequestrin content and SERCA determine normal and maximal Ca2+ storage levels in sarcoplasmic reticulum of fast- and slow-twitch fibres of rat.
    Lamb GD
    The Journal of physiology 587.2 (2009 Jan 15): 443-60.

    Endogenous calpain-3 activation is primarily governed by small increases in resting cytoplasmic [Ca2+] and is not dependent on stretch.
    Lamb GD
    The Journal of biological chemistry 284.12 (2009 Mar 20): 7811-9.

    The regulation of thapsigargin-sensitive sarcoendoplasmic reticulum Ca(2+)-ATPase activity in estivation.
    Storey KB
    Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology 178.1 (2008 Jan): 33-45.

    Taurine supplementation increases skeletal muscle force production and protects muscle function during and after high-frequency in vitro stimulation.
    Hayes A
    Journal of applied physiology (Bethesda, Md. : 1985) 107.1 (2009 Jul): 144-54.

    Activation of skeletal muscle calpain-3 by eccentric exercise in humans does not result in its translocation to the nucleus or cytosol.
    Lamb GD
    Journal of applied physiology (Bethesda, Md. : 1985) 111.5 (2011 Nov): 1448-58.

    Changes in glycolytic network and mitochondrial design in creatine kinase-deficient muscles.
    Wieringa B
    Muscle & nerve 24.9 (2001 Sep): 1188-96.

    Enhanced technique to measure proteins in single segments of human skeletal muscle fibers: fiber-type dependence of AMPK-alpha1 and -beta1.
    Murphy RM
    Journal of applied physiology (Bethesda, Md. : 1985) 110.3 (2011 Mar): 820-5.

    IHC References
    IP References

    The amino-terminal 200 amino acids of the plasma membrane Na+,K+-ATPase alpha subunit confer ouabain sensitivity on the sarcoplasmic reticulum Ca(2+)-ATPase.
    Takeyasu K
    Proceedings of the National Academy of Sciences of the United States of America 90.19 (1993 Oct 1): 8881-5.

    ELISA References
    All References

    Ca2+ homeostasis in Brody's disease. A study in skeletal muscle and cultured muscle cells and the effects of dantrolene an verapamil.
    Wevers RA
    The Journal of clinical investigation 94.2 (1994 Aug): 741-8.

    Dysferlin and myoferlin regulate transverse tubule formation and glycerol sensitivity.
    McNally EM
    The American journal of pathology 184.1 (2014 Jan): 248-59.

    The amino-terminal 200 amino acids of the plasma membrane Na+,K+-ATPase alpha subunit confer ouabain sensitivity on the sarcoplasmic reticulum Ca(2+)-ATPase.
    Takeyasu K
    Proceedings of the National Academy of Sciences of the United States of America 90.19 (1993 Oct 1): 8881-5.

    Correct targeting of dihydropyridine receptors and triadin in dyspedic mouse skeletal muscle in vivo.
    Franzini-Armstrong C
    Developmental dynamics : an official publication of the American Association of Anatomists 214.4 (1999 Apr): 372-80.

    The effect of taurine and β-alanine supplementation on taurine transporter protein and fatigue resistance in skeletal muscle from mdx mice.
    Goodman CA
    Amino acids 48.11 (2016 Nov): 2635-2645.

    Enhanced technique to measure proteins in single segments of human skeletal muscle fibers: fiber-type dependence of AMPK-alpha1 and -beta1.
    Murphy RM
    Journal of applied physiology (Bethesda, Md. : 1985) 110.3 (2011 Mar): 820-5.

    Calsequestrin content and SERCA determine normal and maximal Ca2+ storage levels in sarcoplasmic reticulum of fast- and slow-twitch fibres of rat.
    Lamb GD
    The Journal of physiology 587.2 (2009 Jan 15): 443-60.

    Endogenous calpain-3 activation is primarily governed by small increases in resting cytoplasmic [Ca2+] and is not dependent on stretch.
    Lamb GD
    The Journal of biological chemistry 284.12 (2009 Mar 20): 7811-9.

    The regulation of thapsigargin-sensitive sarcoendoplasmic reticulum Ca(2+)-ATPase activity in estivation.
    Storey KB
    Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology 178.1 (2008 Jan): 33-45.

    Taurine supplementation increases skeletal muscle force production and protects muscle function during and after high-frequency in vitro stimulation.
    Hayes A
    Journal of applied physiology (Bethesda, Md. : 1985) 107.1 (2009 Jul): 144-54.

    Activation of skeletal muscle calpain-3 by eccentric exercise in humans does not result in its translocation to the nucleus or cytosol.
    Lamb GD
    Journal of applied physiology (Bethesda, Md. : 1985) 111.5 (2011 Nov): 1448-58.

    Changes in glycolytic network and mitochondrial design in creatine kinase-deficient muscles.
    Wieringa B
    Muscle & nerve 24.9 (2001 Sep): 1188-96.

    Expression of fast and slow isoforms of the Ca2+-ATPase in developing chick skeletal muscle.
    Fambrough DM
    Developmental biology 124.2 (1987 Dec): 490-503.

    Failure to make normal alpha ryanodine receptor is an early event associated with the crooked neck dwarf (cn) mutation in chicken.
    Talvenheimo J
    Developmental dynamics : an official publication of the American Association of Anatomists 197.3 (1993 Jul): 169-88.

    Crooked neck dwarf (cn) mutant chicken skeletal muscle cells in low density primary cultures fail to express normal alpha ryanodine receptor and exhibit a partial mutant phenotype.
    Sutko JL
    Developmental dynamics : an official publication of the American Association of Anatomists 197.3 (1993 Jul): 189-202.

    Expression of avian Ca2+-ATPase in cultured mouse myogenic cells.
    Fambrough DM
    Molecular and cellular biology 9.5 (1989 May): 1978-86.

    Immunohistochemical evidence for expression of fast-twitch type sarco(endo)plasmic reticulum Ca2+ ATPase (SERCA1) in German shepherd dogs with dilated cardiomyopathy myocardium.
    Young IS
    Journal of veterinary cardiology : the official journal of the European Society of Veterinary Cardiology 12.1 (2010 Apr): 17-23.

    Carboxy-terminal regions of the sarcoplasmic/endoplasmic reticulum Ca(2+)- and the Na+/K(+)-ATPases control their K+ sensitivity.
    Takeyasu K
    Biochemistry 36.2 (1997 Jan 14): 442-51.

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