40.3A4

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$40.00
SKU: 40.3A4
View product citations for antibody 40.3A4 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: islet-1 homeobox
Hybridoma Cells Available: Yes
Antigen Species: rat
Depositor: Jessell, T.M. / Brenner-Morton, S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: HHMI/Columbia University
Positive Tested Species Reactivity: Chicken, Drosophila, Human, Mouse, Planaria, Rat
Depositors Notes: The 40.3A4 monoclonal antibody is often combined with monoclonal antibodies 39.3F7, 39.4D5, and 40.2D6 to create a efficient cocktail for ChIP and/or gel shift [PMID 19502415].
Antigen Molecular Weight: Predicted: 39kDa; Apparent: 39kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Isl1
Immunogen: C-terminal of rat Islet-1 (aa 178-349); recombinant .
Alternate Gene Names: Isl-1; isl-1=homeobox
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS-1
Epitope Location or Sequence:
Uniprot ID: P61374 
Immunogen Sequence: Recombinant C-terminal of rat Islet-1 (aa 178-349)
Entrez Gene ID: 64444 
Additional Characterization:
Antibody Registry ID: AB_528313 
Additional Information:
Recommended Applications: Chromatin Immunoprecipitation, FFPE, Gel Supershift, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
40.3A4 was deposited to the DSHB by Jessell, T.M. / Brenner-Morton, S. (DSHB Hybridoma Product 40.3A4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

27 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FFPE References
  • Epitope Map References
  • Gel Supershift References
  • All References
  • Initial Publication

    Science (New York, N.Y.)
    Yamada T
    Science (New York, N.Y.) 256.5063 (1992 Jun 12): 1555-60.

    IF References



    . (): .

    Genesis (New York, N.Y. : 2000)
    Sánchez Alvarado A
    Genesis (New York, N.Y. : 2000) 32.4 (2002 Apr): 293-8.

    Development (Cambridge, England)
    Modolell J
    Development (Cambridge, England) 134.9 (2007 May): 1779-88.

    PloS one
    Luo J
    PloS one 9.1 (2014): e84617.

    Development (Cambridge, England)
    Nakamura H
    Development (Cambridge, England) 131.3 (2004 Feb): 681-92.

    Developmental biology
    Heitzler P
    Developmental biology 288.2 (2005 Dec 15): 559-70.

    Stem cell research
    Wu W
    Stem cell research 11.1 (2013 Jul): 529-39.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Tole S
    The Journal of neuroscience : the official journal of the Society for Neuroscience 28.37 (2008 Sep 10): 9145-50.

    Cell and tissue research
    Jagla K
    Cell and tissue research 354.2 (2013 Nov): 639-45.

    Journal of the Association for Research in Otolaryngology : JARO
    Heller S
    Journal of the Association for Research in Otolaryngology : JARO 10.2 (2009 Jun): 173-90.

    Gene expression patterns : GEP
    Funahashi J
    Gene expression patterns : GEP 7.4 (2007 Feb): 375-80.

    BMC developmental biology
    Heller S
    BMC developmental biology 5. (2005 Aug 17): 16.

    The Journal of comparative neurology
    Heller S
    The Journal of comparative neurology 477.1 (2004 Sep 6): 1-10.

    Mechanisms of development
    Modolell J
    Mechanisms of development 127.9-12 (2010 Sep-Dec): 393-406.

    Human molecular genetics
    Swaroop A
    Human molecular genetics 22.11 (2013 Jun 1): 2234-46.

    The Journal of cell biology
    Kawamoto S
    The Journal of cell biology 200.4 (2013 Feb 18): 443-58.

    Development (Cambridge, England)
    Pashmforoush M
    Development (Cambridge, England) 141.15 (2014 Aug): 2959-71.

    Human molecular genetics
    Swaroop A
    Human molecular genetics 21.8 (2012 Apr 15): 1848-60.

    WB References

    Developmental biology
    Heitzler P
    Developmental biology 288.2 (2005 Dec 15): 559-70.

    IHC References

    Development (Cambridge, England)
    Nakamura H
    Development (Cambridge, England) 131.3 (2004 Feb): 681-92.

    The American journal of surgical pathology
    Perren A
    The American journal of surgical pathology 32.3 (2008 Mar): 420-5.

    Endocrine pathology
    Dhall D
    Endocrine pathology 24.4 (2013 Dec): 184-90.

    Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc
    Dhall D
    Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 25.6 (2012 Jun): 893-901.

    FFPE References

    The American journal of surgical pathology
    Perren A
    The American journal of surgical pathology 32.3 (2008 Mar): 420-5.

    Endocrine pathology
    Dhall D
    Endocrine pathology 24.4 (2013 Dec): 184-90.

    Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc
    Dhall D
    Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 25.6 (2012 Jun): 893-901.

    Epitope Map References

    Science (New York, N.Y.)
    Yamada T
    Science (New York, N.Y.) 256.5063 (1992 Jun 12): 1555-60.

    Neuron
    Edlund T
    Neuron 7.6 (1991 Dec): 881-9.

    Gel Supershift References

    Diabetes
    May CL
    Diabetes 63.12 (2014 Dec): 4206-17.

    Diabetes
    May CL
    Diabetes 58.9 (2009 Sep): 2059-69.

    All References

    Development (Cambridge, England)
    Nakamura H
    Development (Cambridge, England) 131.3 (2004 Feb): 681-92.

    The American journal of surgical pathology
    Perren A
    The American journal of surgical pathology 32.3 (2008 Mar): 420-5.

    Endocrine pathology
    Dhall D
    Endocrine pathology 24.4 (2013 Dec): 184-90.

    Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc
    Dhall D
    Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 25.6 (2012 Jun): 893-901.



    . (): .

    Genesis (New York, N.Y. : 2000)
    Sánchez Alvarado A
    Genesis (New York, N.Y. : 2000) 32.4 (2002 Apr): 293-8.

    Development (Cambridge, England)
    Modolell J
    Development (Cambridge, England) 134.9 (2007 May): 1779-88.

    PloS one
    Luo J
    PloS one 9.1 (2014): e84617.

    Developmental biology
    Heitzler P
    Developmental biology 288.2 (2005 Dec 15): 559-70.

    Stem cell research
    Wu W
    Stem cell research 11.1 (2013 Jul): 529-39.

    The Journal of neuroscience : the official journal of the Society for Neuroscience
    Tole S
    The Journal of neuroscience : the official journal of the Society for Neuroscience 28.37 (2008 Sep 10): 9145-50.

    Cell and tissue research
    Jagla K
    Cell and tissue research 354.2 (2013 Nov): 639-45.

    Journal of the Association for Research in Otolaryngology : JARO
    Heller S
    Journal of the Association for Research in Otolaryngology : JARO 10.2 (2009 Jun): 173-90.

    Gene expression patterns : GEP
    Funahashi J
    Gene expression patterns : GEP 7.4 (2007 Feb): 375-80.

    BMC developmental biology
    Heller S
    BMC developmental biology 5. (2005 Aug 17): 16.

    The Journal of comparative neurology
    Heller S
    The Journal of comparative neurology 477.1 (2004 Sep 6): 1-10.

    Mechanisms of development
    Modolell J
    Mechanisms of development 127.9-12 (2010 Sep-Dec): 393-406.

    Human molecular genetics
    Swaroop A
    Human molecular genetics 22.11 (2013 Jun 1): 2234-46.

    The Journal of cell biology
    Kawamoto S
    The Journal of cell biology 200.4 (2013 Feb 18): 443-58.

    Development (Cambridge, England)
    Pashmforoush M
    Development (Cambridge, England) 141.15 (2014 Aug): 2959-71.

    Human molecular genetics
    Swaroop A
    Human molecular genetics 21.8 (2012 Apr 15): 1848-60.

    Science (New York, N.Y.)
    Yamada T
    Science (New York, N.Y.) 256.5063 (1992 Jun 12): 1555-60.

    Neuron
    Edlund T
    Neuron 7.6 (1991 Dec): 881-9.

    Diabetes
    May CL
    Diabetes 63.12 (2014 Dec): 4206-17.

    Diabetes
    May CL
    Diabetes 58.9 (2009 Sep): 2059-69.

    Development (Cambridge, England)
    Barde YA
    Development (Cambridge, England) 131.21 (2004 Nov): 5449-56.

    Methods (San Diego, Calif.)
    Bodmer R
    Methods (San Diego, Calif.) 68.1 (2014 Jun 15): 265-72.

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