40.3A4
Catalog Fields
Antigen: islet-1 homeobox
Hybridoma Cells Available: Yes
Antigen Species: rat
Depositor: Jessell, T.M. / Brenner-Morton, S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: HHMI/Columbia University
Positive Tested Species Reactivity: Chicken, Drosophila, Human, Mouse, Planaria, Rat
Depositors Notes: The 40.3A4 monoclonal antibody is often combined with monoclonal antibodies 39.3F7, 39.4D5, and 40.2D6 to create a efficient cocktail for ChIP and/or gel shift [PMID 19502415].
Antigen Molecular Weight: Predicted: 39kDa; Apparent: 39kDa
Human Protein Atlas:
Predicted Species Reactivity:
Gene: Isl1
Immunogen: C-terminal of rat Islet-1 (aa 178-349); recombinant .
Alternate Gene Names: Isl-1; isl-1=homeobox
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS-1
Epitope Location or Sequence:
Uniprot ID: P61374
Immunogen Sequence: Recombinant C-terminal of rat Islet-1 (aa 178-349)
Entrez Gene ID: 64444
Additional Characterization:
Antibody Registry ID: AB_528313
Additional Information:
Recommended Applications: Chromatin Immunoprecipitation, FFPE, Gel Supershift, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
40.3A4 was deposited to the DSHB by Jessell, T.M. / Brenner-Morton, S. (DSHB Hybridoma Product 40.3A4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
27 References
Initial Publication
Early stages of motor neuron differentiation revealed by expression of homeobox gene Islet-1.
Yamada T
Science (New York, N.Y.) 256.5063 (1992 Jun 12): 1555-60.