40.3A4

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$40.00
SKU: 40.3A4
View product citations for antibody 40.3A4 on CiteAb

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Available: 200

DSHB Data Sheet

Catalog Fields

Antigen: islet-1 homeobox
Hybridoma Cells Available: Yes
Antigen Species: rat
Depositor: Jessell, T.M. / Brenner-Morton, S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: HHMI/Columbia University
Positive Tested Species Reactivity: Chicken, Drosophila, Human, Mouse, Planaria, Rat
Depositors Notes: The 40.3A4 monoclonal antibody is often combined with monoclonal antibodies 39.3F7, 39.4D5, and 40.2D6 to create a efficient cocktail for ChIP and/or gel shift [PMID 19502415].
Antigen Molecular Weight: Predicted: 39kDa; Apparent: 39kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Isl1
Immunogen: C-terminal of rat Islet-1 (aa 178-349); recombinant .
Alternate Gene Names: Isl-1; isl-1=homeobox
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS-1
Epitope Location or Sequence:
Uniprot ID: P61374 
Immunogen Sequence: Recombinant C-terminal of rat Islet-1 (aa 178-349)
Entrez Gene ID: 64444 
Additional Characterization:
Antibody Registry ID: AB_528313 
Additional Information:
Recommended Applications: Chromatin Immunoprecipitation, FFPE, Gel Supershift, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
40.3A4 was deposited to the DSHB by Jessell, T.M. / Brenner-Morton, S. (DSHB Hybridoma Product 40.3A4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

27 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FFPE References
  • Epitope Map References
  • Gel Supershift References
  • All References
  • Initial Publication

    Early stages of motor neuron differentiation revealed by expression of homeobox gene Islet-1.
    Yamada T
    Science (New York, N.Y.) 256.5063 (1992 Jun 12): 1555-60.

    IF References



    . (): .

    Identification of immunological reagents for use in the study of freshwater planarians by means of whole-mount immunofluorescence and confocal microscopy.
    Sánchez Alvarado A
    Genesis (New York, N.Y. : 2000) 32.4 (2002 Apr): 293-8.

    tailup, a LIM-HD gene, and Iro-C cooperate in Drosophila dorsal mesothorax specification.
    Modolell J
    Development (Cambridge, England) 134.9 (2007 May): 1779-88.

    ADAM10 negatively regulates neuronal differentiation during spinal cord development.
    Luo J
    PloS one 9.1 (2014): e84617.

    Navigation of trochlear motor axons along the midbrain-hindbrain boundary by neuropilin 2.
    Nakamura H
    Development (Cambridge, England) 131.3 (2004 Feb): 681-92.

    The Drosophila LIM-homeo domain protein Islet antagonizes pro-neural cell specification in the peripheral nervous system.
    Heitzler P
    Developmental biology 288.2 (2005 Dec 15): 559-70.

    Transplanted motoneurons derived from human induced pluripotent stem cells form functional connections with target muscle.
    Wu W
    Stem cell research 11.1 (2013 Jul): 529-39.

    Chip is required for posteclosion behavior in Drosophila.
    Tole S
    The Journal of neuroscience : the official journal of the Society for Neuroscience 28.37 (2008 Sep 10): 9145-50.

    Tailup plays multiple roles during cardiac outflow assembly in Drosophila.
    Jagla K
    Cell and tissue research 354.2 (2013 Nov): 639-45.

    Stem/progenitor cells derived from the cochlear sensory epithelium give rise to spheres with distinct morphologies and features.
    Heller S
    Journal of the Association for Research in Otolaryngology : JARO 10.2 (2009 Jun): 173-90.

    Cloning and developmental expression of a chick G-protein-coupled receptor SCGPR1.
    Funahashi J
    Gene expression patterns : GEP 7.4 (2007 Feb): 375-80.

    BMP4 signaling is involved in the generation of inner ear sensory epithelia.
    Heller S
    BMC developmental biology 5. (2005 Aug 17): 16.

    Islet-1 expression in the developing chicken inner ear.
    Heller S
    The Journal of comparative neurology 477.1 (2004 Sep 6): 1-10.

    The pronotum LIM-HD gene tailup is both a positive and a negative regulator of the proneural genes achaete and scute of Drosophila.
    Modolell J
    Mechanisms of development 127.9-12 (2010 Sep-Dec): 393-406.

    Prickle1 is expressed in distinct cell populations of the central nervous system and contributes to neuronal morphogenesis.
    Swaroop A
    Human molecular genetics 22.11 (2013 Jun 1): 2234-46.

    Rbfox3-regulated alternative splicing of Numb promotes neuronal differentiation during development.
    Kawamoto S
    The Journal of cell biology 200.4 (2013 Feb 18): 443-58.

    Nkx2-5 regulates cardiac growth through modulation of Wnt signaling by R-spondin3.
    Pashmforoush M
    Development (Cambridge, England) 141.15 (2014 Aug): 2959-71.

    Regulation of retinal progenitor expansion by Frizzled receptors: implications for microphthalmia and retinal coloboma.
    Swaroop A
    Human molecular genetics 21.8 (2012 Apr 15): 1848-60.

    WB References
    IHC References
    FFPE References
    Epitope Map References
    Gel Supershift References
    All References

    Navigation of trochlear motor axons along the midbrain-hindbrain boundary by neuropilin 2.
    Nakamura H
    Development (Cambridge, England) 131.3 (2004 Feb): 681-92.

    Islet 1 (Isl1) expression is a reliable marker for pancreatic endocrine tumors and their metastases.
    Perren A
    The American journal of surgical pathology 32.3 (2008 Mar): 420-5.

    The immunohistochemical expression of islet 1 and PAX8 by rectal neuroendocrine tumors should be taken into account in the differential diagnosis of metastatic neuroendocrine tumors of unknown primary origin.
    Dhall D
    Endocrine pathology 24.4 (2013 Dec): 184-90.

    Value of Islet 1 and PAX8 in identifying metastatic neuroendocrine tumors of pancreatic origin.
    Dhall D
    Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 25.6 (2012 Jun): 893-901.



    . (): .

    Identification of immunological reagents for use in the study of freshwater planarians by means of whole-mount immunofluorescence and confocal microscopy.
    Sánchez Alvarado A
    Genesis (New York, N.Y. : 2000) 32.4 (2002 Apr): 293-8.

    tailup, a LIM-HD gene, and Iro-C cooperate in Drosophila dorsal mesothorax specification.
    Modolell J
    Development (Cambridge, England) 134.9 (2007 May): 1779-88.

    ADAM10 negatively regulates neuronal differentiation during spinal cord development.
    Luo J
    PloS one 9.1 (2014): e84617.

    The Drosophila LIM-homeo domain protein Islet antagonizes pro-neural cell specification in the peripheral nervous system.
    Heitzler P
    Developmental biology 288.2 (2005 Dec 15): 559-70.

    Transplanted motoneurons derived from human induced pluripotent stem cells form functional connections with target muscle.
    Wu W
    Stem cell research 11.1 (2013 Jul): 529-39.

    Chip is required for posteclosion behavior in Drosophila.
    Tole S
    The Journal of neuroscience : the official journal of the Society for Neuroscience 28.37 (2008 Sep 10): 9145-50.

    Tailup plays multiple roles during cardiac outflow assembly in Drosophila.
    Jagla K
    Cell and tissue research 354.2 (2013 Nov): 639-45.

    Stem/progenitor cells derived from the cochlear sensory epithelium give rise to spheres with distinct morphologies and features.
    Heller S
    Journal of the Association for Research in Otolaryngology : JARO 10.2 (2009 Jun): 173-90.

    Cloning and developmental expression of a chick G-protein-coupled receptor SCGPR1.
    Funahashi J
    Gene expression patterns : GEP 7.4 (2007 Feb): 375-80.

    BMP4 signaling is involved in the generation of inner ear sensory epithelia.
    Heller S
    BMC developmental biology 5. (2005 Aug 17): 16.

    Islet-1 expression in the developing chicken inner ear.
    Heller S
    The Journal of comparative neurology 477.1 (2004 Sep 6): 1-10.

    The pronotum LIM-HD gene tailup is both a positive and a negative regulator of the proneural genes achaete and scute of Drosophila.
    Modolell J
    Mechanisms of development 127.9-12 (2010 Sep-Dec): 393-406.

    Prickle1 is expressed in distinct cell populations of the central nervous system and contributes to neuronal morphogenesis.
    Swaroop A
    Human molecular genetics 22.11 (2013 Jun 1): 2234-46.

    Rbfox3-regulated alternative splicing of Numb promotes neuronal differentiation during development.
    Kawamoto S
    The Journal of cell biology 200.4 (2013 Feb 18): 443-58.

    Nkx2-5 regulates cardiac growth through modulation of Wnt signaling by R-spondin3.
    Pashmforoush M
    Development (Cambridge, England) 141.15 (2014 Aug): 2959-71.

    Regulation of retinal progenitor expansion by Frizzled receptors: implications for microphthalmia and retinal coloboma.
    Swaroop A
    Human molecular genetics 21.8 (2012 Apr 15): 1848-60.

    Early stages of motor neuron differentiation revealed by expression of homeobox gene Islet-1.
    Yamada T
    Science (New York, N.Y.) 256.5063 (1992 Jun 12): 1555-60.

    The homeodomain LIM protein Isl-1 is expressed in subsets of neurons and endocrine cells in the adult rat.
    Edlund T
    Neuron 7.6 (1991 Dec): 881-9.

    Islet-1 Is essential for pancreatic β-cell function.
    May CL
    Diabetes 63.12 (2014 Dec): 4206-17.

    Islet-1 is required for the maturation, proliferation, and survival of the endocrine pancreas.
    May CL
    Diabetes 58.9 (2009 Sep): 2059-69.

    Developmental potential of defined neural progenitors derived from mouse embryonic stem cells.
    Barde YA
    Development (Cambridge, England) 131.21 (2004 Nov): 5449-56.

    Methods to assess Drosophila heart development, function and aging.
    Bodmer R
    Methods (San Diego, Calif.) 68.1 (2014 Jun 15): 265-72.

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