40.3A4
Catalog Fields
Antigen: islet-1 homeobox
Hybridoma Cells Available: Yes
Antigen Species: rat
Depositor: Jessell, T.M. / Brenner-Morton, S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Columbia University / HHMI
Positive Tested Species Reactivity: Chicken, Drosophila, Human, Mouse, Planaria, Rat
Depositors Notes: The 40.3A4 monoclonal antibody is often combined with monoclonal antibodies 39.3F7, 39.4D5, and 40.2D6 to create a efficient cocktail for ChIP and/or gel shift [PMID 19502415].
Predicted Species Reactivity:
Gene: Isl1
Immunogen: C-terminal of rat Islet-1 (aa 178-349); recombinant .
Alternate Gene Names: Isl-1; isl-1=homeobox
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS-1
Epitope Location or Sequence:
Uniprot ID: P61374
Immunogen Sequence: Recombinant C-terminal of rat Islet-1 (aa 178-349)
Entrez Gene ID: 64444
Antibody Registry ID: AB_528313
Additional Information:
Recommended Applications: Chromatin Immunoprecipitation, FFPE, Gel Supershift, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
40.3A4 was deposited to the DSHB by Jessell, T.M. / Brenner-Morton, S. (DSHB Hybridoma Product 40.3A4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
29 References
Initial Publication
Early stages of motor neuron differentiation revealed by expression of homeobox gene Islet-1.
Yamada T
Science (New York, N.Y.) 256.5063 (1992 Jun 12): 1555-60.