4G1

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$40.00
SKU: 4G1
View product citations for antibody 4G1 on CiteAb

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Available: 138

DSHB Data Sheet

Catalog Fields

Antigen: MSX1+2
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Jessell, T.M. / Brenner-Morton, S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: HHMI/Columbia University
Positive Tested Species Reactivity: Amphibian, Chicken, Human, Mouse, Rat
Depositors Notes:
Antigen Molecular Weight: Predicted: 30.5 kDa (MSX1-derived protein); 28.2 kDa(MSX2-derived protein)
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: MSX1,MSX2
Immunogen:
Alternate Gene Names: HOX-7; CHOX-7; GHOX-7(for MSX1); HOX-8; Msx-2(for MSX2)
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: NS-1
Epitope Location or Sequence:
Uniprot ID: P28362 
Immunogen Sequence: chick MSX2 bacterially expressed protein
Entrez Gene ID: 396484 395245 
Additional Characterization:
Antibody Registry ID: AB_531788 
Additional Information:
Recommended Applications: Gel Supershift, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
4G1 was deposited to the DSHB by Jessell, T.M. / Brenner-Morton, S. (DSHB Hybridoma Product 4G1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

14 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Gel Supershift References
  • All References
  • Initial Publication
    IF References

    Quantitative evaluation of morpholino-mediated protein knockdown of GFP, MSX1, and PAX7 during tail regeneration in Ambystoma mexicanum.
    Tanaka EM
    Developmental dynamics : an official publication of the American Association of Anatomists 232.1 (2005 Jan): 162-70.

    Lack of motor neuron differentiation is an intrinsic property of the mouse secondary neural tube.
    Roelink H
    Developmental dynamics : an official publication of the American Association of Anatomists 239.12 (2010 Dec): 3192-203.

    Control of roof plate formation by Lmx1a in the developing spinal cord.
    Millen KJ
    Development (Cambridge, England) 131.11 (2004 Jun): 2693-705.

    Analysis of early human neural crest development.
    García-Castro MI
    Developmental biology 344.2 (2010 Aug 15): 578-92.

    FGF and retinoic acid activity gradients control the timing of neural crest cell emigration in the trunk.
    Morales AV
    The Journal of cell biology 194.3 (2011 Aug 8): 489-503.

    PIAS1 confers DNA-binding specificity on the Msx1 homeoprotein.
    Abate-Shen C
    Genes and development 20.7 (2006 Apr 1): 784-94.

    Transgenic Xenopus with prx1 limb enhancer reveals crucial contribution of MEK/ERK and PI3K/AKT pathways in blastema formation during limb regeneration.
    Tamura K
    Developmental biology 304.2 (2007 Apr 15): 675-86.

    Transgenic Xenopus with prx1 limb enhancer reveals crucial contribution of MEK/ERK and PI3K/AKT pathways in blastema formation during limb regeneration.
    Tamura K
    Developmental biology 304.2 (2007 Apr 15): 675-86.

    Identification of Embryonic Neural Plate Border Stem Cells and Their Generation by Direct Reprogramming from Adult Human Blood Cells.
    Trumpp A
    Cell stem cell 24.1 (2019 Jan 3): 166-182.e13.

    WB References
    IHC References

    Border formation in a Bmp gradient reduced to single dissociated cells.
    Monuki ES
    Proceedings of the National Academy of Sciences of the United States of America 105.9 (2008 Mar 4): 3398-403.

    Opposing Fgf and Bmp activities regulate the specification of olfactory sensory and respiratory epithelial cell fates.
    Gunhaga L
    Development (Cambridge, England) 137.10 (2010 May): 1601-11.

    Control of roof plate formation by Lmx1a in the developing spinal cord.
    Millen KJ
    Development (Cambridge, England) 131.11 (2004 Jun): 2693-705.

    Control of roof plate formation by Lmx1a in the developing spinal cord.
    Millen KJ
    Development (Cambridge, England) 131.11 (2004 Jun): 2693-705.

    Gel Supershift References
    All References

    Border formation in a Bmp gradient reduced to single dissociated cells.
    Monuki ES
    Proceedings of the National Academy of Sciences of the United States of America 105.9 (2008 Mar 4): 3398-403.

    Opposing Fgf and Bmp activities regulate the specification of olfactory sensory and respiratory epithelial cell fates.
    Gunhaga L
    Development (Cambridge, England) 137.10 (2010 May): 1601-11.

    Control of roof plate formation by Lmx1a in the developing spinal cord.
    Millen KJ
    Development (Cambridge, England) 131.11 (2004 Jun): 2693-705.

    Quantitative evaluation of morpholino-mediated protein knockdown of GFP, MSX1, and PAX7 during tail regeneration in Ambystoma mexicanum.
    Tanaka EM
    Developmental dynamics : an official publication of the American Association of Anatomists 232.1 (2005 Jan): 162-70.

    Lack of motor neuron differentiation is an intrinsic property of the mouse secondary neural tube.
    Roelink H
    Developmental dynamics : an official publication of the American Association of Anatomists 239.12 (2010 Dec): 3192-203.

    Analysis of early human neural crest development.
    García-Castro MI
    Developmental biology 344.2 (2010 Aug 15): 578-92.

    FGF and retinoic acid activity gradients control the timing of neural crest cell emigration in the trunk.
    Morales AV
    The Journal of cell biology 194.3 (2011 Aug 8): 489-503.

    PIAS1 confers DNA-binding specificity on the Msx1 homeoprotein.
    Abate-Shen C
    Genes and development 20.7 (2006 Apr 1): 784-94.

    Transgenic Xenopus with prx1 limb enhancer reveals crucial contribution of MEK/ERK and PI3K/AKT pathways in blastema formation during limb regeneration.
    Tamura K
    Developmental biology 304.2 (2007 Apr 15): 675-86.

    Identification of Embryonic Neural Plate Border Stem Cells and Their Generation by Direct Reprogramming from Adult Human Blood Cells.
    Trumpp A
    Cell stem cell 24.1 (2019 Jan 3): 166-182.e13.

    BMP4 and PTHrP interact to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction.
    Wysolmerski J
    Development (Cambridge, England) 134.6 (2007 Mar): 1221-30.

    Dlx3 transcriptional regulation of osteoblast differentiation: temporal recruitment of Msx2, Dlx3, and Dlx5 homeodomain proteins to chromatin of the osteocalcin gene.
    Lian JB
    Molecular and cellular biology 24.20 (2004 Oct): 9248-61.

    BMP2 commitment to the osteogenic lineage involves activation of Runx2 by DLX3 and a homeodomain transcriptional network.
    Lian JB
    The Journal of biological chemistry 281.52 (2006 Dec 29): 40515-26.

    Dorsal differentiation of neural plate cells induced by BMP-mediated signals from epidermal ectoderm.
    Jessell TM
    Cell 82.6 (1995 Sep 22): 969-79.

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