8C3

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$40.00
SKU: 8C3
View product citations for antibody 8C3 on CiteAb

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Available: 125

DSHB Data Sheet

Catalog Fields

Antigen: Syntaxin
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Benzer, S. / Colley, N.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: California Institute of Technology
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: 35 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Syx1A
Immunogen: Heads(Drosophila melanogaster), homogenized.
Alternate Gene Names: Dm Syx1; DmSyx1A; dsyn-1A; dSyx1; Syt1A;
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: NS-1
Epitope Location or Sequence:
Uniprot ID: Q24547 
Immunogen Sequence:
Entrez Gene ID: 42854 
Additional Characterization:
Antibody Registry ID: AB_528484 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
8C3 was deposited to the DSHB by Benzer, S. / Colley, N. (DSHB Hybridoma Product 8C3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

47 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • All References
  • Initial Publication

    Monoclonal antibodies against the Drosophila nervous system.
    Shotwell SL
    Proceedings of the National Academy of Sciences of the United States of America 79.24 (1982 Dec): 7929-33.

    IF References
    WB References

    gcm2 promotes glial cell differentiation and is required with glial cells missing for macrophage development in Drosophila.
    Jones BW
    Developmental biology 248.2 (2002 Aug 15): 369-83.

    dAcsl, the Drosophila ortholog of acyl-CoA synthetase long-chain family member 3 and 4, inhibits synapse growth by attenuating bone morphogenetic protein signaling via endocytic recycling.
    Zhang YQ
    The Journal of neuroscience : the official journal of the Society for Neuroscience 34.8 (2014 Feb 19): 2785-96.

    A novel Drosophila model of TDP-43 proteinopathies: N-terminal sequences combined with the Q/N domain induce protein functional loss and locomotion defects.
    Baralle FE
    Disease models and mechanisms 9.6 (2016 Jun 1): 659-69.

    The Gos28 SNARE protein mediates intra-Golgi transport of rhodopsin and is required for photoreceptor survival.
    Colley NJ
    The Journal of biological chemistry 289.47 (2014 Nov 21): 32392-409.

    Inhibitors of endocytosis prevent Wnt/Wingless signalling by reducing the level of basal β-catenin/Armadillo.
    Vincent JP
    Journal of cell science 127.Pt 22 (2014 Nov 15): 4918-26.

    Drosophila 14-3-3ε has a crucial role in anti-microbial peptide secretion and innate immunity.
    Lopez AF
    Journal of cell science 124.Pt 13 (2011 Jul 1): 2165-74.

    Molecular motor function in axonal transport in vivo probed by genetic and computational analysis in Drosophila.
    Goldstein LS
    Molecular biology of the cell 23.9 (2012 May): 1700-14.

    Differential effects of Tau on the integrity and function of neurons essential for learning in Drosophila.
    Skoulakis EM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.2 (2010 Jan 13): 464-77.

    Epithelial septate junction assembly relies on melanotransferrin iron binding and endocytosis in Drosophila.
    Samakovlis C
    Nature cell biology 12.11 (2010 Nov): 1071-7.

    Molecular mechanisms of COMPLEXIN fusion clamp function in synaptic exocytosis revealed in a new Drosophila mutant.
    Kawasaki F
    Molecular and cellular neurosciences 56. (2013 Sep): 244-54.

    Dimerization is essential for 14-3-3zeta stability and function in vivo.
    Skoulakis EM
    The Journal of biological chemistry 285.3 (2010 Jan 15): 1692-700.

    SIDL interacts with the dendritic targeting motif of Shal (K(v)4) K+ channels in Drosophila.
    Tsunoda S
    Molecular and cellular neurosciences 45.1 (2010 Sep): 75-83.

    The v-ATPase V0 subunit a1 is required for a late step in synaptic vesicle exocytosis in Drosophila.
    Bellen HJ
    Cell 121.4 (2005 May 20): 607-620.

    The synaptic vesicle SNARE neuronal Synaptobrevin promotes endolysosomal degradation and prevents neurodegeneration.
    Hiesinger PR
    The Journal of cell biology 196.2 (2012 Jan 23): 261-76.

    Drosophila Rae1 controls the abundance of the ubiquitin ligase Highwire in post-mitotic neurons.
    Wu C
    Nature neuroscience 14.10 (2011 Aug 28): 1267-75.

    Interkingdom complementation reveals structural conservation and functional divergence of 14-3-3 proteins.
    Skoulakis EM
    PloS one 8.10 (2013): e78090.

    Large-scale screen for modifiers of ataxin-3-derived polyglutamine-induced toxicity in Drosophila.
    Voigt A
    PloS one 7.11 (2012): e47452.

    Activity-dependent interactions of NSF and SNAP at living synapses.
    Ordway RW
    Molecular and cellular neurosciences 47.1 (2011 May): 19-27.

    Modification of a hydrophobic layer by a point mutation in syntaxin 1A regulates the rate of synaptic vesicle fusion.
    Zhang B
    PLoS biology 5.4 (2007 Apr): e72.

    Cell type-specific processing of human Tau proteins in Drosophila.
    Skoulakis EM
    FEBS letters 580.19 (2006 Aug 21): 4602-6.

    Dynactin is required for coordinated bidirectional motility, but not for dynein membrane attachment.
    Goldstein LS
    Molecular biology of the cell 18.6 (2007 Jun): 2081-9.

    Rolling blackout is required for synaptic vesicle exocytosis.
    Broadie K
    The Journal of neuroscience : the official journal of the Society for Neuroscience 26.9 (2006 Mar 1): 2369-79.

    Neurexin-1 is required for synapse formation and larvae associative learning in Drosophila.
    Xie W
    FEBS letters 581.13 (2007 May 29): 2509-16.

    A fluorescent sphingolipid binding domain peptide probe interacts with sphingolipids and cholesterol-dependent raft domains.
    Kraut R
    Journal of lipid research 49.5 (2008 May): 1077-89.

    Two distinct effects on neurotransmission in a temperature-sensitive SNAP-25 mutant.
    Deitcher DL
    The EMBO journal 20.23 (2001 Dec 3): 6761-71.

    An essential role of Rab5 in uniformity of synaptic vesicle size.
    Ozaki K
    Journal of cell science 116.Pt 17 (2003 Sep 1): 3583-90.

    SNARE proteins contribute to calcium cooperativity of synaptic transmission.
    Boulianne GL
    Proceedings of the National Academy of Sciences of the United States of America 97.25 (2000 Dec 5): 13955-60.

    Unc-51 controls active zone density and protein composition by downregulating ERK signaling.
    Diantonio A
    The Journal of neuroscience : the official journal of the Society for Neuroscience 29.2 (2009 Jan 14): 517-28.

    Analysis of the mutant Drosophila N-ethylmaleimide sensitive fusion-1 protein in comatose reveals molecular correlates of the behavioural paralysis.
    Trimble WS
    Journal of neurochemistry 77.5 (2001 Jun): 1407-17.

    Overexpression of cysteine-string proteins in Drosophila reveals interactions with syntaxin.
    Zinsmaier KE
    The Journal of neuroscience : the official journal of the Society for Neuroscience 19.23 (1999 Dec 1): 10270-9.

    Temperature-sensitive paralytic mutations demonstrate that synaptic exocytosis requires SNARE complex assembly and disassembly.
    Ganetzky B
    Neuron 21.2 (1998 Aug): 401-13.

    NSF function in neurotransmitter release involves rearrangement of the SNARE complex downstream of synaptic vesicle docking.
    Pallanck L
    The Journal of neuroscience : the official journal of the Society for Neuroscience 18.24 (1998 Dec 15): 10250-6.

    The synaptic protein syntaxin1 is required for cellularization of Drosophila embryos.
    Schwarz TL
    The Journal of cell biology 138.4 (1997 Aug 25): 861-75.

    ADAMTS Sol narae cleaves extracellular Wingless to generate a novel active form that regulates cell proliferation in Drosophila.
    Cho KO
    Cell death and disease 10.8 (2019 Jul 22): 564.

    IHC References

    Monoclonal antibodies against the Drosophila nervous system.
    Shotwell SL
    Proceedings of the National Academy of Sciences of the United States of America 79.24 (1982 Dec): 7929-33.

    Drosophila spichthyin inhibits BMP signaling and regulates synaptic growth and axonal microtubules.
    O'Kane CJ
    Nature neuroscience 10.2 (2007 Feb): 177-85.

    Drosophila syntaxin is required for cell viability and may function in membrane formation and stabilization.
    Bellen HJ
    Genetics 144.4 (1996 Dec): 1713-24.

    Visualization of synaptic markers in the optic neuropils of Drosophila using a new constrained deconvolution method.
    Obermayer K
    The Journal of comparative neurology 429.2 (2001 Jan 8): 277-88.

    Spatial and temporal characteristics of normal and perturbed vesicle transport.
    Gunawardena S
    PloS one 9.5 (2014): e97237.

    Ca2+-Calmodulin regulates SNARE assembly and spontaneous neurotransmitter release via v-ATPase subunit V0a1.
    Hiesinger PR
    The Journal of cell biology 205.1 (2014 Apr 14): 21-31.

    Drosophila Syd-1, liprin-α, and protein phosphatase 2A B' subunit Wrd function in a linear pathway to prevent ectopic accumulation of synaptic materials in distal axons.
    Wu C
    The Journal of neuroscience : the official journal of the Society for Neuroscience 34.25 (2014 Jun 18): 8474-87.

    Synaptic PI(3,4,5)P3 is required for Syntaxin1A clustering and neurotransmitter release.
    Verstreken P
    Neuron 77.6 (2013 Mar 20): 1097-108.

    Neural lineages of the Drosophila brain: a three-dimensional digital atlas of the pattern of lineage location and projection at the late larval stage.
    Hartenstein V
    The Journal of neuroscience : the official journal of the Society for Neuroscience 26.20 (2006 May 17): 5534-53.

    Rab6 mediates membrane organization and determinant localization during Drosophila oogenesis.
    Ephrussi A
    Development (Cambridge, England) 134.7 (2007 Apr): 1419-30.

    Do SNARE proteins confer specificity for vesicle fusion?
    Zhang B
    Proceedings of the National Academy of Sciences of the United States of America 99.21 (2002 Oct 15): 13359-61.

    A Drosophila SNAP-25 null mutant reveals context-dependent redundancy with SNAP-24 in neurotransmission.
    Deitcher DL
    Genetics 162.1 (2002 Sep): 259-71.

    The synaptic protein syntaxin1 is required for cellularization of Drosophila embryos.
    Schwarz TL
    The Journal of cell biology 138.4 (1997 Aug 25): 861-75.

    IP References

    The v-ATPase V0 subunit a1 is required for a late step in synaptic vesicle exocytosis in Drosophila.
    Bellen HJ
    Cell 121.4 (2005 May 20): 607-620.

    Ca2+-Calmodulin regulates SNARE assembly and spontaneous neurotransmitter release via v-ATPase subunit V0a1.
    Hiesinger PR
    The Journal of cell biology 205.1 (2014 Apr 14): 21-31.

    The synaptic vesicle SNARE neuronal Synaptobrevin promotes endolysosomal degradation and prevents neurodegeneration.
    Hiesinger PR
    The Journal of cell biology 196.2 (2012 Jan 23): 261-76.

    Activity-dependent interactions of NSF and SNAP at living synapses.
    Ordway RW
    Molecular and cellular neurosciences 47.1 (2011 May): 19-27.

    Syntaxin 1A interacts with multiple exocytic proteins to regulate neurotransmitter release in vivo.
    Bellen HJ
    Neuron 23.3 (1999 Jul): 593-605.

    Overexpression of cysteine-string proteins in Drosophila reveals interactions with syntaxin.
    Zinsmaier KE
    The Journal of neuroscience : the official journal of the Society for Neuroscience 19.23 (1999 Dec 1): 10270-9.

    All References

    Monoclonal antibodies against the Drosophila nervous system.
    Shotwell SL
    Proceedings of the National Academy of Sciences of the United States of America 79.24 (1982 Dec): 7929-33.

    Drosophila spichthyin inhibits BMP signaling and regulates synaptic growth and axonal microtubules.
    O'Kane CJ
    Nature neuroscience 10.2 (2007 Feb): 177-85.

    Drosophila syntaxin is required for cell viability and may function in membrane formation and stabilization.
    Bellen HJ
    Genetics 144.4 (1996 Dec): 1713-24.

    Visualization of synaptic markers in the optic neuropils of Drosophila using a new constrained deconvolution method.
    Obermayer K
    The Journal of comparative neurology 429.2 (2001 Jan 8): 277-88.

    Spatial and temporal characteristics of normal and perturbed vesicle transport.
    Gunawardena S
    PloS one 9.5 (2014): e97237.

    Ca2+-Calmodulin regulates SNARE assembly and spontaneous neurotransmitter release via v-ATPase subunit V0a1.
    Hiesinger PR
    The Journal of cell biology 205.1 (2014 Apr 14): 21-31.

    Drosophila Syd-1, liprin-α, and protein phosphatase 2A B' subunit Wrd function in a linear pathway to prevent ectopic accumulation of synaptic materials in distal axons.
    Wu C
    The Journal of neuroscience : the official journal of the Society for Neuroscience 34.25 (2014 Jun 18): 8474-87.

    Synaptic PI(3,4,5)P3 is required for Syntaxin1A clustering and neurotransmitter release.
    Verstreken P
    Neuron 77.6 (2013 Mar 20): 1097-108.

    Neural lineages of the Drosophila brain: a three-dimensional digital atlas of the pattern of lineage location and projection at the late larval stage.
    Hartenstein V
    The Journal of neuroscience : the official journal of the Society for Neuroscience 26.20 (2006 May 17): 5534-53.

    Rab6 mediates membrane organization and determinant localization during Drosophila oogenesis.
    Ephrussi A
    Development (Cambridge, England) 134.7 (2007 Apr): 1419-30.

    Do SNARE proteins confer specificity for vesicle fusion?
    Zhang B
    Proceedings of the National Academy of Sciences of the United States of America 99.21 (2002 Oct 15): 13359-61.

    A Drosophila SNAP-25 null mutant reveals context-dependent redundancy with SNAP-24 in neurotransmission.
    Deitcher DL
    Genetics 162.1 (2002 Sep): 259-71.

    The synaptic protein syntaxin1 is required for cellularization of Drosophila embryos.
    Schwarz TL
    The Journal of cell biology 138.4 (1997 Aug 25): 861-75.

    Drosophila 14-3-3ε has a crucial role in anti-microbial peptide secretion and innate immunity.
    Lopez AF
    Journal of cell science 124.Pt 13 (2011 Jul 1): 2165-74.

    Neurexin-1 is required for synapse formation and larvae associative learning in Drosophila.
    Xie W
    FEBS letters 581.13 (2007 May 29): 2509-16.

    A novel Drosophila model of TDP-43 proteinopathies: N-terminal sequences combined with the Q/N domain induce protein functional loss and locomotion defects.
    Baralle FE
    Disease models and mechanisms 9.6 (2016 Jun 1): 659-69.

    gcm2 promotes glial cell differentiation and is required with glial cells missing for macrophage development in Drosophila.
    Jones BW
    Developmental biology 248.2 (2002 Aug 15): 369-83.

    dAcsl, the Drosophila ortholog of acyl-CoA synthetase long-chain family member 3 and 4, inhibits synapse growth by attenuating bone morphogenetic protein signaling via endocytic recycling.
    Zhang YQ
    The Journal of neuroscience : the official journal of the Society for Neuroscience 34.8 (2014 Feb 19): 2785-96.

    The Gos28 SNARE protein mediates intra-Golgi transport of rhodopsin and is required for photoreceptor survival.
    Colley NJ
    The Journal of biological chemistry 289.47 (2014 Nov 21): 32392-409.

    Inhibitors of endocytosis prevent Wnt/Wingless signalling by reducing the level of basal β-catenin/Armadillo.
    Vincent JP
    Journal of cell science 127.Pt 22 (2014 Nov 15): 4918-26.

    Molecular motor function in axonal transport in vivo probed by genetic and computational analysis in Drosophila.
    Goldstein LS
    Molecular biology of the cell 23.9 (2012 May): 1700-14.

    Differential effects of Tau on the integrity and function of neurons essential for learning in Drosophila.
    Skoulakis EM
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.2 (2010 Jan 13): 464-77.

    Epithelial septate junction assembly relies on melanotransferrin iron binding and endocytosis in Drosophila.
    Samakovlis C
    Nature cell biology 12.11 (2010 Nov): 1071-7.

    Molecular mechanisms of COMPLEXIN fusion clamp function in synaptic exocytosis revealed in a new Drosophila mutant.
    Kawasaki F
    Molecular and cellular neurosciences 56. (2013 Sep): 244-54.

    Dimerization is essential for 14-3-3zeta stability and function in vivo.
    Skoulakis EM
    The Journal of biological chemistry 285.3 (2010 Jan 15): 1692-700.

    SIDL interacts with the dendritic targeting motif of Shal (K(v)4) K+ channels in Drosophila.
    Tsunoda S
    Molecular and cellular neurosciences 45.1 (2010 Sep): 75-83.

    The v-ATPase V0 subunit a1 is required for a late step in synaptic vesicle exocytosis in Drosophila.
    Bellen HJ
    Cell 121.4 (2005 May 20): 607-620.

    The synaptic vesicle SNARE neuronal Synaptobrevin promotes endolysosomal degradation and prevents neurodegeneration.
    Hiesinger PR
    The Journal of cell biology 196.2 (2012 Jan 23): 261-76.

    Drosophila Rae1 controls the abundance of the ubiquitin ligase Highwire in post-mitotic neurons.
    Wu C
    Nature neuroscience 14.10 (2011 Aug 28): 1267-75.

    Interkingdom complementation reveals structural conservation and functional divergence of 14-3-3 proteins.
    Skoulakis EM
    PloS one 8.10 (2013): e78090.

    Large-scale screen for modifiers of ataxin-3-derived polyglutamine-induced toxicity in Drosophila.
    Voigt A
    PloS one 7.11 (2012): e47452.

    Activity-dependent interactions of NSF and SNAP at living synapses.
    Ordway RW
    Molecular and cellular neurosciences 47.1 (2011 May): 19-27.

    Modification of a hydrophobic layer by a point mutation in syntaxin 1A regulates the rate of synaptic vesicle fusion.
    Zhang B
    PLoS biology 5.4 (2007 Apr): e72.

    Cell type-specific processing of human Tau proteins in Drosophila.
    Skoulakis EM
    FEBS letters 580.19 (2006 Aug 21): 4602-6.

    Dynactin is required for coordinated bidirectional motility, but not for dynein membrane attachment.
    Goldstein LS
    Molecular biology of the cell 18.6 (2007 Jun): 2081-9.

    Rolling blackout is required for synaptic vesicle exocytosis.
    Broadie K
    The Journal of neuroscience : the official journal of the Society for Neuroscience 26.9 (2006 Mar 1): 2369-79.

    A fluorescent sphingolipid binding domain peptide probe interacts with sphingolipids and cholesterol-dependent raft domains.
    Kraut R
    Journal of lipid research 49.5 (2008 May): 1077-89.

    Two distinct effects on neurotransmission in a temperature-sensitive SNAP-25 mutant.
    Deitcher DL
    The EMBO journal 20.23 (2001 Dec 3): 6761-71.

    An essential role of Rab5 in uniformity of synaptic vesicle size.
    Ozaki K
    Journal of cell science 116.Pt 17 (2003 Sep 1): 3583-90.

    SNARE proteins contribute to calcium cooperativity of synaptic transmission.
    Boulianne GL
    Proceedings of the National Academy of Sciences of the United States of America 97.25 (2000 Dec 5): 13955-60.

    Unc-51 controls active zone density and protein composition by downregulating ERK signaling.
    Diantonio A
    The Journal of neuroscience : the official journal of the Society for Neuroscience 29.2 (2009 Jan 14): 517-28.

    Analysis of the mutant Drosophila N-ethylmaleimide sensitive fusion-1 protein in comatose reveals molecular correlates of the behavioural paralysis.
    Trimble WS
    Journal of neurochemistry 77.5 (2001 Jun): 1407-17.

    Overexpression of cysteine-string proteins in Drosophila reveals interactions with syntaxin.
    Zinsmaier KE
    The Journal of neuroscience : the official journal of the Society for Neuroscience 19.23 (1999 Dec 1): 10270-9.

    Temperature-sensitive paralytic mutations demonstrate that synaptic exocytosis requires SNARE complex assembly and disassembly.
    Ganetzky B
    Neuron 21.2 (1998 Aug): 401-13.

    NSF function in neurotransmitter release involves rearrangement of the SNARE complex downstream of synaptic vesicle docking.
    Pallanck L
    The Journal of neuroscience : the official journal of the Society for Neuroscience 18.24 (1998 Dec 15): 10250-6.

    ADAMTS Sol narae cleaves extracellular Wingless to generate a novel active form that regulates cell proliferation in Drosophila.
    Cho KO
    Cell death and disease 10.8 (2019 Jul 22): 564.

    Syntaxin 1A interacts with multiple exocytic proteins to regulate neurotransmitter release in vivo.
    Bellen HJ
    Neuron 23.3 (1999 Jul): 593-605.

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