8C3

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SKU: 8C3

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Supernatant 1.0 ml Concentrate 0.1 ml Frozen Cells 1 vial Growing Cells 1 vial

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DSHB Data Sheet

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Clone ID/Product Name: 8C3

Available to For-Profits: Yes

Alternate Antibody Name:

Gene Symbol: Syx1A

Ab Isotype: MIgG1, kappa light chain

Gene Name:

Antibody Registry ID: AB_528484

Uniprot ID: Q24547

RRID:

Entrez Gene ID: 42854

Clonality: Monoclonal

Immunogen: adult fly heads homogenates

Clone:

Immunogen Sequence: Full length sequence

Myeloma Strain: NS-1

Epitope Mapped: No

Antigen Name: Syntaxin

Epitope Location or Sequence:

Alternate Antigen Name:

Deposit Date: 8/27/1998

Antigen Molecular Weight: 35 kDa

Depositor: Benzer, S. / Colley, N.

Antigen Sequence:

Depositor Institution: California Institute of Technology, Division of Biology

Antigen Species: Drosophila

Depositor Notes:

Host Species: mouse

Hybridoma Cells Available (Non-Profit): Yes

Confirmed Species Reactivity: Drosophila, Mosquito

Additional Information: RRID: AB_528484. FBgn0013343

Predicted Species Reactivity:

Human Protein Atlas:

Additional Characterization:

Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

All cell products contain the antimicrobial ProClin. Click here for additional information.

These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.

For your Materials & Methods section:

8C3 was deposited to the DSHB by Benzer, S. / Colley, N. (DSHB Hybridoma Product 8C3)

Storage and Handling Recommendations

Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.

Usage Recommendations

The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

58 References

Initial Publication

IF References

WB References

IHC References

IP References

All References

Initial Publication

Monoclonal antibodies against the Drosophila nervous system.
Shotwell SL
Proceedings of the National Academy of Sciences of the United States of America 79.24 (1982 Dec): 7929-33.

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8C3

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