4C5

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$40.00
SKU: 4C5
View product citations for antibody 4C5 on CiteAb

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Available: 208

DSHB Data Sheet

Catalog Fields

Antigen: rhodopsin 1 (Rh1 Drosophila)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: de Couet, H.G. / Tanimura, T.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Hawaii
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: Predicted: 39 kDa,
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: ninaE
Immunogen: Rhodopsin (1-6) Drosophila melanogaster; recombinant
Alternate Gene Names: Dm Rh1; DMELRH1; Dmel\ Nina E; opsin; Rh1/ninaE; rhodopsin; Rhodopsin-1C-beta
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NA
Epitope Location or Sequence:
Uniprot ID: P06002 
Immunogen Sequence:
Entrez Gene ID: 42367 
Additional Characterization:  
Antibody Registry ID: AB_528451 
Additional Information:
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
4C5 was deposited to the DSHB by de Couet, H.G. / Tanimura, T. (DSHB Hybridoma Product 4C5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

9 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • All References
  • Initial Publication
    IF References

    Phosphatidic acid phospholipase A1 mediates ER-Golgi transit of a family of G protein-coupled receptors.
    Acharya JK
    The Journal of cell biology 206.1 (2014 Jul 7): 79-95.

    Light-induced translocation of Drosophila visual Arrestin2 depends on Rac2.
    Montell C
    Proceedings of the National Academy of Sciences of the United States of America 107.10 (2010 Mar 9): 4740-5.

    WB References
    IHC References

    Light-induced translocation of Drosophila visual Arrestin2 depends on Rac2.
    Montell C
    Proceedings of the National Academy of Sciences of the United States of America 107.10 (2010 Mar 9): 4740-5.

    Spatial and temporal regulation of glycosylation during Drosophila eye development.
    Goto S
    Cell and tissue research 336.1 (2009 Apr): 137-47.

    The adaptor protein X11Lalpha/Dmint1 interacts with the PDZ-binding domain of the cell recognition protein Rst in Drosophila.
    Fischbach KF
    Developmental biology 289.2 (2006 Jan 15): 296-307.

    Eyes closed, a Drosophila p47 homolog, is essential for photoreceptor morphogenesis.
    Ready DF
    Development (Cambridge, England) 129.1 (2002 Jan): 143-54.

    A dPIP5K dependent pool of phosphatidylinositol 4,5 bisphosphate (PIP2) is required for G-protein coupled signal transduction in Drosophila photoreceptors.
    Raghu P
    PLoS genetics 11.1 (2015 Jan): e1004948.

    IP References

    Light-induced translocation of Drosophila visual Arrestin2 depends on Rac2.
    Montell C
    Proceedings of the National Academy of Sciences of the United States of America 107.10 (2010 Mar 9): 4740-5.

    Phosphatidic acid phospholipase A1 mediates ER-Golgi transit of a family of G protein-coupled receptors.
    Acharya JK
    The Journal of cell biology 206.1 (2014 Jul 7): 79-95.

    Light-induced translocation of Drosophila visual Arrestin2 depends on Rac2.
    Montell C
    Proceedings of the National Academy of Sciences of the United States of America 107.10 (2010 Mar 9): 4740-5.

    All References

    Light-induced translocation of Drosophila visual Arrestin2 depends on Rac2.
    Montell C
    Proceedings of the National Academy of Sciences of the United States of America 107.10 (2010 Mar 9): 4740-5.

    Spatial and temporal regulation of glycosylation during Drosophila eye development.
    Goto S
    Cell and tissue research 336.1 (2009 Apr): 137-47.

    The adaptor protein X11Lalpha/Dmint1 interacts with the PDZ-binding domain of the cell recognition protein Rst in Drosophila.
    Fischbach KF
    Developmental biology 289.2 (2006 Jan 15): 296-307.

    Eyes closed, a Drosophila p47 homolog, is essential for photoreceptor morphogenesis.
    Ready DF
    Development (Cambridge, England) 129.1 (2002 Jan): 143-54.

    A dPIP5K dependent pool of phosphatidylinositol 4,5 bisphosphate (PIP2) is required for G-protein coupled signal transduction in Drosophila photoreceptors.
    Raghu P
    PLoS genetics 11.1 (2015 Jan): e1004948.

    Phosphatidic acid phospholipase A1 mediates ER-Golgi transit of a family of G protein-coupled receptors.
    Acharya JK
    The Journal of cell biology 206.1 (2014 Jul 7): 79-95.

    GPI biosynthesis is essential for rhodopsin sorting at the trans-Golgi network in Drosophila photoreceptors.
    Satoh AK
    Development (Cambridge, England) 140.2 (2013 Jan 15): 385-94.

    Essential function of Drosophila Sec6 in apical exocytosis of epithelial photoreceptor cells.
    Tepass U
    The Journal of cell biology 169.4 (2005 May 23): 635-46.

    Regulation of the rhodopsin protein phosphatase, RDGC, through interaction with calmodulin.
    Montell C
    Neuron 32.6 (2001 Dec 20): 1097-106.

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