4C5

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SKU: 4C5

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DSHB Data Sheet

Catalog Fields

Product Name/ID: 4C5
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: ninaE
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_528451 
Uniprot ID: P06002 
RRID:  
Entrez Gene ID: 42367 
Clonality: Monoclonal
Immunogen: Rhodopsin (1-6) Drosophila melanogaster; recombinant
Clone:
Immunogen Sequence:
Myeloma Strain: NA
Epitope Mapped: No
Antigen Name: rhodopsin 1 (Rh1 Drosophila)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 1/18/1996
Antigen Molecular Weight: Predicted: 39 kDa,
Depositor: de Couet, H.G. / Tanimura, T.
Antigen Sequence:
Depositor Institution: University of Hawaii
Antigen Species: Drosophila
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
4C5 was deposited to the DSHB by de Couet, H.G. / Tanimura, T. (DSHB Hybridoma Product 4C5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

10 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • All References
  • Initial Publication
    IF References

    Phosphatidic acid phospholipase A1 mediates ER-Golgi transit of a family of G protein-coupled receptors.
    Acharya JK
    The Journal of cell biology 206.1 (2014 Jul 7): 79-95.

    Light-induced translocation of Drosophila visual Arrestin2 depends on Rac2.
    Montell C
    Proceedings of the National Academy of Sciences of the United States of America 107.10 (2010 Mar 9): 4740-5.

    Mutations in the splicing regulator Prp31 lead to retinal degeneration in Drosophila.
    Knust E
    Biology open 10.1 (2021 Jan 25): .

    WB References
    IHC References

    Light-induced translocation of Drosophila visual Arrestin2 depends on Rac2.
    Montell C
    Proceedings of the National Academy of Sciences of the United States of America 107.10 (2010 Mar 9): 4740-5.

    Spatial and temporal regulation of glycosylation during Drosophila eye development.
    Goto S
    Cell and tissue research 336.1 (2009 Apr): 137-47.

    The adaptor protein X11Lalpha/Dmint1 interacts with the PDZ-binding domain of the cell recognition protein Rst in Drosophila.
    Fischbach KF
    Developmental biology 289.2 (2006 Jan 15): 296-307.

    Eyes closed, a Drosophila p47 homolog, is essential for photoreceptor morphogenesis.
    Ready DF
    Development (Cambridge, England) 129.1 (2002 Jan): 143-54.

    A dPIP5K dependent pool of phosphatidylinositol 4,5 bisphosphate (PIP2) is required for G-protein coupled signal transduction in Drosophila photoreceptors.
    Raghu P
    PLoS genetics 11.1 (2015 Jan): e1004948.

    IP References

    Light-induced translocation of Drosophila visual Arrestin2 depends on Rac2.
    Montell C
    Proceedings of the National Academy of Sciences of the United States of America 107.10 (2010 Mar 9): 4740-5.

    Phosphatidic acid phospholipase A1 mediates ER-Golgi transit of a family of G protein-coupled receptors.
    Acharya JK
    The Journal of cell biology 206.1 (2014 Jul 7): 79-95.

    Light-induced translocation of Drosophila visual Arrestin2 depends on Rac2.
    Montell C
    Proceedings of the National Academy of Sciences of the United States of America 107.10 (2010 Mar 9): 4740-5.

    All References

    Light-induced translocation of Drosophila visual Arrestin2 depends on Rac2.
    Montell C
    Proceedings of the National Academy of Sciences of the United States of America 107.10 (2010 Mar 9): 4740-5.

    Spatial and temporal regulation of glycosylation during Drosophila eye development.
    Goto S
    Cell and tissue research 336.1 (2009 Apr): 137-47.

    The adaptor protein X11Lalpha/Dmint1 interacts with the PDZ-binding domain of the cell recognition protein Rst in Drosophila.
    Fischbach KF
    Developmental biology 289.2 (2006 Jan 15): 296-307.

    Eyes closed, a Drosophila p47 homolog, is essential for photoreceptor morphogenesis.
    Ready DF
    Development (Cambridge, England) 129.1 (2002 Jan): 143-54.

    A dPIP5K dependent pool of phosphatidylinositol 4,5 bisphosphate (PIP2) is required for G-protein coupled signal transduction in Drosophila photoreceptors.
    Raghu P
    PLoS genetics 11.1 (2015 Jan): e1004948.

    Phosphatidic acid phospholipase A1 mediates ER-Golgi transit of a family of G protein-coupled receptors.
    Acharya JK
    The Journal of cell biology 206.1 (2014 Jul 7): 79-95.

    Mutations in the splicing regulator Prp31 lead to retinal degeneration in Drosophila.
    Knust E
    Biology open 10.1 (2021 Jan 25): .

    GPI biosynthesis is essential for rhodopsin sorting at the trans-Golgi network in Drosophila photoreceptors.
    Satoh AK
    Development (Cambridge, England) 140.2 (2013 Jan 15): 385-94.

    Essential function of Drosophila Sec6 in apical exocytosis of epithelial photoreceptor cells.
    Tepass U
    The Journal of cell biology 169.4 (2005 May 23): 635-46.

    Regulation of the rhodopsin protein phosphatase, RDGC, through interaction with calmodulin.
    Montell C
    Neuron 32.6 (2001 Dec 20): 1097-106.

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