6B9

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$40.00
SKU: 6B9
View product citations for antibody 6B9 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Villin-like protein quail
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Cooley, L.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Yale University Medical School
Positive Tested Species Reactivity: Drosophila
Depositors Notes: quail 6B9 (concentrated) (villin-like): Use 1:50 in Blotto-Tween (PBS, 5% dry milk, 0.2% Tween) for Westerns or 1:50 in PBT (PBS, 0.3% Triton-X-100, and 0.5% BSA) for ovary staining. (Mahajan-Miklos and Cooley, 1994).
Antigen Molecular Weight: 98 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: qua
Immunogen: Recombinant 6XHis-Quail fusion protein
Alternate Gene Names: Dmel_CG6433
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID: Q23989 
Immunogen Sequence: Full length protein
Entrez Gene ID: 35058 
Additional Characterization:  
Antibody Registry ID: AB_528447 
Additional Information: RRID:AB_528447 FBgn0003187.
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
6B9 was deposited to the DSHB by Cooley, L. (DSHB Hybridoma Product 6B9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

12 References

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication
    IF References

    The villin-like protein encoded by the Drosophila quail gene is required for actin bundle assembly during oogenesis.
    Cooley L
    Cell 78.2 (1994 Jul 29): 291-301.

    Drosophila fascin mutants are rescued by overexpression of the villin-like protein, quail.
    Cooley L
    Journal of cell science 111 ( Pt 2). (1998 Jan): 213-21.

    Drosophila quail, a villin-related protein, bundles actin filaments in apoptotic nurse cells.
    Cooley L
    Development (Cambridge, England) 126.24 (1999 Dec): 5645-57.

    Mutations in the midway gene disrupt a Drosophila acyl coenzyme A: diacylglycerol acyltransferase.
    Cooley L
    Genetics 160.4 (2002 Apr): 1511-8.

    Actin filament bundles in Drosophila wing hairs: hairs and bristles use different strategies for assembly.
    Tilney LG
    Molecular biology of the cell 16.8 (2005 Aug): 3620-31.

    Drosophila Fascin is a novel downstream target of prostaglandin signaling during actin remodeling.
    Tootle TL
    Molecular biology of the cell 23.23 (2012 Dec): 4567-78.

    Systematic analysis of the transcriptional switch inducing migration of border cells.
    Rørth P
    Developmental cell 10.4 (2006 Apr): 497-508.

    Microvilli appear to represent the first step in actin bundle formation in Drosophila bristles.
    Guild GM
    Journal of cell science 117.Pt 16 (2004 Jul 15): 3531-8.

    Prostaglandins regulate nuclear localization of Fascin and its function in nucleolar architecture.
    Tootle TL
    Molecular biology of the cell 26.10 (2015 May 15): 1901-17.

    Prostaglandins temporally regulate cytoplasmic actin bundle formation during Drosophila oogenesis.
    Tootle TL
    Molecular biology of the cell 25.3 (2014 Feb): 397-411.

    Capping protein beta is required for actin cytoskeleton organisation and cell migration during Drosophila oogenesis.
    Baricheva EM
    Cell biology international 37.2 (2013 Feb): 149-59.

    Bicaudal C and trailer hitch have similar roles in gurken mRNA localization and cytoskeletal organization.
    Macdonald PM
    Developmental biology 328.2 (2009 Apr 15): 434-44.

    WB References
    All References

    The villin-like protein encoded by the Drosophila quail gene is required for actin bundle assembly during oogenesis.
    Cooley L
    Cell 78.2 (1994 Jul 29): 291-301.

    Drosophila fascin mutants are rescued by overexpression of the villin-like protein, quail.
    Cooley L
    Journal of cell science 111 ( Pt 2). (1998 Jan): 213-21.

    Drosophila quail, a villin-related protein, bundles actin filaments in apoptotic nurse cells.
    Cooley L
    Development (Cambridge, England) 126.24 (1999 Dec): 5645-57.

    Mutations in the midway gene disrupt a Drosophila acyl coenzyme A: diacylglycerol acyltransferase.
    Cooley L
    Genetics 160.4 (2002 Apr): 1511-8.

    Actin filament bundles in Drosophila wing hairs: hairs and bristles use different strategies for assembly.
    Tilney LG
    Molecular biology of the cell 16.8 (2005 Aug): 3620-31.

    Drosophila Fascin is a novel downstream target of prostaglandin signaling during actin remodeling.
    Tootle TL
    Molecular biology of the cell 23.23 (2012 Dec): 4567-78.

    Systematic analysis of the transcriptional switch inducing migration of border cells.
    Rørth P
    Developmental cell 10.4 (2006 Apr): 497-508.

    Microvilli appear to represent the first step in actin bundle formation in Drosophila bristles.
    Guild GM
    Journal of cell science 117.Pt 16 (2004 Jul 15): 3531-8.

    Prostaglandins regulate nuclear localization of Fascin and its function in nucleolar architecture.
    Tootle TL
    Molecular biology of the cell 26.10 (2015 May 15): 1901-17.

    Prostaglandins temporally regulate cytoplasmic actin bundle formation during Drosophila oogenesis.
    Tootle TL
    Molecular biology of the cell 25.3 (2014 Feb): 397-411.

    Capping protein beta is required for actin cytoskeleton organisation and cell migration during Drosophila oogenesis.
    Baricheva EM
    Cell biology international 37.2 (2013 Feb): 149-59.

    Bicaudal C and trailer hitch have similar roles in gurken mRNA localization and cytoskeletal organization.
    Macdonald PM
    Developmental biology 328.2 (2009 Apr 15): 434-44.

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