Cq4

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$40.00
SKU: Cq4
View product citations for antibody Cq4 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Crumbs Drosophila protein (extracellular domain)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Knust, E.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Max Planck Institute of Molecular Cell Biology and Genetics
Positive Tested Species Reactivity: Drosophila melanogaster, Drosophila virilis
Depositors Notes: crossreacts with Drosophila virilis, not with mouse or C. elegans. Crumbs protein is expressed in all epithelia of ectodermal origin in the Drosophila embryo from gastrulation onwards and in all imaginal discs and the follicle epithelium. It is restricted to a region apical to the zonula adherens. Good apical marker for epithelial cells.
Antigen Molecular Weight: Predicted: 234 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: crb
Immunogen: Recombinant fusion protein between beta-galactosidase and aa 737-1703 of Drosophila Crumbs protein.
Alternate Gene Names: CG6383
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS-1
Epitope Location or Sequence: aa 737-1703
Uniprot ID: P10040 
Immunogen Sequence: Partial protein
Entrez Gene ID: 42896 
Additional Characterization:
Antibody Registry ID: AB_528181 
Additional Information: For experiments involving live cells and tissue, Cq4 supernatant is available without the antimicrobial ProClin. Contact us at 319-335-3826 or dshb@uiowa.edu for special ordering instructions.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Cq4 was deposited to the DSHB by Knust, E. (DSHB Hybridoma Product Cq4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

19 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Posterior midgut epithelial cells differ in their organization of the membrane skeleton from other drosophila epithelia.
    Baumann O
    Experimental cell research 270.2 (2001 Nov 1): 176-87.

    aPKC phosphorylation of Bazooka defines the apical/lateral border in Drosophila epithelial cells.
    St Johnston D
    Cell 141.3 (2010 Apr 30): 509-23.

    Reiterative use of signalling pathways controls multiple cellular events during Drosophila posterior spiracle organogenesis.
    Graba Y
    Developmental biology 343.1-2 (2010 Jul 1): 18-27.

    CYFIP dependent actin remodeling controls specific aspects of Drosophila eye morphogenesis.
    Giangrande A
    Developmental biology 359.1 (2011 Nov 1): 37-46.

    A conserved motif in Crumbs is required for E-cadherin localisation and zonula adherens formation in Drosophila.
    Knust E
    Current biology : CB 10.2 (2000 Jan 27): 76-85.

    Dynein-mediated apical localization of crumbs transcripts is required for Crumbs activity in epithelial polarity.
    Cai Y
    The Journal of cell biology 180.1 (2008 Jan 14): 31-8.

    Brahma regulates the Hippo pathway activity through forming complex with Yki-Sd and regulating the transcription of Crumbs.
    Zhang P
    Cellular signalling 27.3 (2015 Mar): 606-13.

    Computer modelling in combination with in vitro studies reveals similar binding affinities of Drosophila Crumbs for the PDZ domains of Stardust and DmPar-6.
    Knust E
    European journal of cell biology 85.8 (2006 Aug): 753-67.

    Drosophila Stardust is a partner of Crumbs in the control of epithelial cell polarity.
    Knust E
    Nature 414.6864 (2001 Dec 6): 638-43.

    Multiple domains of Stardust differentially mediate localisation of the Crumbs-Stardust complex during photoreceptor development in Drosophila.
    Knust E
    Journal of cell science 121.Pt 12 (2008 Jun 15): 2018-26.

    Reception of Slit requires only the chondroitin-sulphate-modified extracellular domain of Syndecan at the target cell surface.
    Vorbrüggen G
    Proceedings of the National Academy of Sciences of the United States of America 106.29 (2009 Jul 21): 11984-8.

    Dystroglycan is required for polarizing the epithelial cells and the oocyte in Drosophila.
    Ruohola-Baker H
    Development (Cambridge, England) 130.1 (2003 Jan): 173-84.

    Crumbs promotes expanded recognition and degradation by the SCF(Slimb/β-TrCP) ubiquitin ligase.
    Tapon N
    Proceedings of the National Academy of Sciences of the United States of America 111.19 (2014 May 13): E1980-9.

    WB References
    IHC References
    Epitope Map References
    All References

    Crumbs and stardust act in a genetic pathway that controls the organization of epithelia in Drosophila melanogaster.
    Knust E
    Developmental biology 159.1 (1993 Sep): 311-26.

    The function of the neurogenic genes during epithelial development in the Drosophila embryo.
    Hartenstein V
    Development (Cambridge, England) 116.4 (1992 Dec): 1203-20.

    Drosophila Stardust is a partner of Crumbs in the control of epithelial cell polarity.
    Knust E
    Nature 414.6864 (2001 Dec 6): 638-43.

    Dystroglycan is required for polarizing the epithelial cells and the oocyte in Drosophila.
    Ruohola-Baker H
    Development (Cambridge, England) 130.1 (2003 Jan): 173-84.

    shotgun encodes Drosophila E-cadherin and is preferentially required during cell rearrangement in the neurectoderm and other morphogenetically active epithelia.
    Hartenstein V
    Genes and development 10.6 (1996 Mar 15): 672-85.

    Cdc42 defines apical identity and regulates epithelial morphogenesis by promoting apical recruitment of Par6-aPKC and Crumbs.
    Pichaud F
    Development (Cambridge, England) 146.15 (2019 Aug 12): .

    Posterior midgut epithelial cells differ in their organization of the membrane skeleton from other drosophila epithelia.
    Baumann O
    Experimental cell research 270.2 (2001 Nov 1): 176-87.

    aPKC phosphorylation of Bazooka defines the apical/lateral border in Drosophila epithelial cells.
    St Johnston D
    Cell 141.3 (2010 Apr 30): 509-23.

    Reiterative use of signalling pathways controls multiple cellular events during Drosophila posterior spiracle organogenesis.
    Graba Y
    Developmental biology 343.1-2 (2010 Jul 1): 18-27.

    CYFIP dependent actin remodeling controls specific aspects of Drosophila eye morphogenesis.
    Giangrande A
    Developmental biology 359.1 (2011 Nov 1): 37-46.

    A conserved motif in Crumbs is required for E-cadherin localisation and zonula adherens formation in Drosophila.
    Knust E
    Current biology : CB 10.2 (2000 Jan 27): 76-85.

    Dynein-mediated apical localization of crumbs transcripts is required for Crumbs activity in epithelial polarity.
    Cai Y
    The Journal of cell biology 180.1 (2008 Jan 14): 31-8.

    Brahma regulates the Hippo pathway activity through forming complex with Yki-Sd and regulating the transcription of Crumbs.
    Zhang P
    Cellular signalling 27.3 (2015 Mar): 606-13.

    Computer modelling in combination with in vitro studies reveals similar binding affinities of Drosophila Crumbs for the PDZ domains of Stardust and DmPar-6.
    Knust E
    European journal of cell biology 85.8 (2006 Aug): 753-67.

    Multiple domains of Stardust differentially mediate localisation of the Crumbs-Stardust complex during photoreceptor development in Drosophila.
    Knust E
    Journal of cell science 121.Pt 12 (2008 Jun 15): 2018-26.

    Reception of Slit requires only the chondroitin-sulphate-modified extracellular domain of Syndecan at the target cell surface.
    Vorbrüggen G
    Proceedings of the National Academy of Sciences of the United States of America 106.29 (2009 Jul 21): 11984-8.

    Crumbs promotes expanded recognition and degradation by the SCF(Slimb/β-TrCP) ubiquitin ligase.
    Tapon N
    Proceedings of the National Academy of Sciences of the United States of America 111.19 (2014 May 13): E1980-9.

    Retromer regulates apical-basal polarity through recycling Crumbs.
    Lin X
    Developmental biology 360.1 (2011 Dec 1): 87-95.

    Crumbs, a component of the apical membrane, is required for zonula adherens formation in primary epithelia of Drosophila.
    Tepass U
    Developmental biology 177.1 (1996 Jul 10): 217-25.

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