DCAT-1

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$40.00
SKU: DCAT-1
View product citations for antibody DCAT-1 on CiteAb

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Available: 110

DSHB Data Sheet

Catalog Fields

Antigen: catenin, alpha- (Drosophila)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Takeichi, M.
Isotype: RIgG2a, kappa light chain
Antigen Sequence:
Host Species: rat
Depositors Institution: RIKEN Center for Developmental Biology
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: Predicted: 102 kDa; Apparent: 110 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: alpha-Cat
Immunogen: Recombinant Drosophila alpha-catenin fused with the T7 phage gene 10 protein.
Alternate Gene Names: CG17947, ?cat, D?-cat, a-cat, ?-catenin
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3U1
Epitope Location or Sequence: aa 240-910
Uniprot ID: P35220 
Immunogen Sequence: Partial protein
Entrez Gene ID: 40517 
Additional Characterization:
Antibody Registry ID: AB_532377 
Additional Information:
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DCAT-1 was deposited to the DSHB by Takeichi, M. (DSHB Hybridoma Product DCAT-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

16 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Identification of a Drosophila homologue of alpha-catenin and its association with the armadillo protein.
    Takeichi M
    The Journal of cell biology 121.5 (1993 Jun): 1133-40.

    Dynamic features of adherens junctions during Drosophila embryonic epithelial morphogenesis revealed by a Dalpha-catenin-GFP fusion protein.
    Tsukita S
    Development genes and evolution 209.4 (1999 Apr): 218-25.

    A Drosophila homolog of cadherin associated with armadillo and essential for embryonic cell-cell adhesion.
    Takeichi M
    Developmental biology 165.2 (1994 Oct): 716-26.

    Crumbs, a component of the apical membrane, is required for zonula adherens formation in primary epithelia of Drosophila.
    Tepass U
    Developmental biology 177.1 (1996 Jul 10): 217-25.

    Mutational analysis supports a core role for Drosophila α-catenin in adherens junction function.
    Tepass U
    Journal of cell science 125.Pt 1 (2012 Jan 1): 233-45.

    Direct binding between two PDZ domain proteins Canoe and ZO-1 and their roles in regulation of the jun N-terminal kinase pathway in Drosophila morphogenesis.
    Yamamoto D
    Mechanisms of development 78.1-2 (1998 Nov): 97-111.

    Phenotypic analysis of null mutants for DE-cadherin and Armadillo in Drosophila ovaries reveals distinct aspects of their functions in cell adhesion and cytoskeletal organization.
    Takeichi M
    Genes to cells : devoted to molecular and cellular mechanisms 2.1 (1997 Jan): 29-40.

    Male-killing Spiroplasma induces sex-specific cell death via host apoptotic pathway.
    Fukatsu T
    PLoS pathogens 10.2 (2014 Feb): e1003956.

    Unexpectedly robust assembly of the Axin destruction complex regulates Wnt/Wg signaling in Drosophila as revealed by analysis in vivo.
    Wehrli M
    Developmental biology 320.1 (2008 Aug 1): 226-41.

    AIP1 acts with cofilin to control actin dynamics during epithelial morphogenesis.
    Chen J
    Development (Cambridge, England) 139.19 (2012 Oct): 3561-71.

    The glucosyltransferase Xiantuan of the endoplasmic reticulum specifically affects E-Cadherin expression and is required for gastrulation movements in Drosophila.
    Großhans J
    Developmental biology 390.2 (2014 Jun 15): 208-20.

    Girdin-mediated interactions between cadherin and the actin cytoskeleton are required for epithelial morphogenesis in Drosophila.
    Laprise P
    Development (Cambridge, England) 142.10 (2015 May 15): 1777-84.

    Modulation of dADAR-dependent RNA editing by the Drosophila fragile X mental retardation protein.
    Jongens TA
    Nature neuroscience 14.12 (2011 Oct 30): 1517-24.

    WB References

    Identification of a Drosophila homologue of alpha-catenin and its association with the armadillo protein.
    Takeichi M
    The Journal of cell biology 121.5 (1993 Jun): 1133-40.

    Dynamic features of adherens junctions during Drosophila embryonic epithelial morphogenesis revealed by a Dalpha-catenin-GFP fusion protein.
    Tsukita S
    Development genes and evolution 209.4 (1999 Apr): 218-25.

    A Drosophila homolog of cadherin associated with armadillo and essential for embryonic cell-cell adhesion.
    Takeichi M
    Developmental biology 165.2 (1994 Oct): 716-26.

    Mutational analysis supports a core role for Drosophila α-catenin in adherens junction function.
    Tepass U
    Journal of cell science 125.Pt 1 (2012 Jan 1): 233-45.

    Distinct Rap1 activity states control the extent of epithelial invagination via α-catenin.
    Wieschaus EF
    Developmental cell 25.3 (2013 May 13): 299-309.

    Unexpectedly robust assembly of the Axin destruction complex regulates Wnt/Wg signaling in Drosophila as revealed by analysis in vivo.
    Wehrli M
    Developmental biology 320.1 (2008 Aug 1): 226-41.

    A genome-wide screen identifies conserved protein hubs required for cadherin-mediated cell-cell adhesion.
    Nelson WJ
    The Journal of cell biology 204.2 (2014 Jan 20): 265-79.

    The glucosyltransferase Xiantuan of the endoplasmic reticulum specifically affects E-Cadherin expression and is required for gastrulation movements in Drosophila.
    Großhans J
    Developmental biology 390.2 (2014 Jun 15): 208-20.

    Girdin-mediated interactions between cadherin and the actin cytoskeleton are required for epithelial morphogenesis in Drosophila.
    Laprise P
    Development (Cambridge, England) 142.10 (2015 May 15): 1777-84.

    Modulation of dADAR-dependent RNA editing by the Drosophila fragile X mental retardation protein.
    Jongens TA
    Nature neuroscience 14.12 (2011 Oct 30): 1517-24.

    Dynamic microtubules produce an asymmetric E-cadherin-Bazooka complex to maintain segment boundaries.
    Brown NH
    The Journal of cell biology 201.6 (2013 Jun 10): 887-901.

    IP References
    Epitope Map References
    All References

    Identification of a Drosophila homologue of alpha-catenin and its association with the armadillo protein.
    Takeichi M
    The Journal of cell biology 121.5 (1993 Jun): 1133-40.

    Dynamic features of adherens junctions during Drosophila embryonic epithelial morphogenesis revealed by a Dalpha-catenin-GFP fusion protein.
    Tsukita S
    Development genes and evolution 209.4 (1999 Apr): 218-25.

    A Drosophila homolog of cadherin associated with armadillo and essential for embryonic cell-cell adhesion.
    Takeichi M
    Developmental biology 165.2 (1994 Oct): 716-26.

    Crumbs, a component of the apical membrane, is required for zonula adherens formation in primary epithelia of Drosophila.
    Tepass U
    Developmental biology 177.1 (1996 Jul 10): 217-25.

    Mutational analysis supports a core role for Drosophila α-catenin in adherens junction function.
    Tepass U
    Journal of cell science 125.Pt 1 (2012 Jan 1): 233-45.

    Direct binding between two PDZ domain proteins Canoe and ZO-1 and their roles in regulation of the jun N-terminal kinase pathway in Drosophila morphogenesis.
    Yamamoto D
    Mechanisms of development 78.1-2 (1998 Nov): 97-111.

    Phenotypic analysis of null mutants for DE-cadherin and Armadillo in Drosophila ovaries reveals distinct aspects of their functions in cell adhesion and cytoskeletal organization.
    Takeichi M
    Genes to cells : devoted to molecular and cellular mechanisms 2.1 (1997 Jan): 29-40.

    Male-killing Spiroplasma induces sex-specific cell death via host apoptotic pathway.
    Fukatsu T
    PLoS pathogens 10.2 (2014 Feb): e1003956.

    Unexpectedly robust assembly of the Axin destruction complex regulates Wnt/Wg signaling in Drosophila as revealed by analysis in vivo.
    Wehrli M
    Developmental biology 320.1 (2008 Aug 1): 226-41.

    AIP1 acts with cofilin to control actin dynamics during epithelial morphogenesis.
    Chen J
    Development (Cambridge, England) 139.19 (2012 Oct): 3561-71.

    The glucosyltransferase Xiantuan of the endoplasmic reticulum specifically affects E-Cadherin expression and is required for gastrulation movements in Drosophila.
    Großhans J
    Developmental biology 390.2 (2014 Jun 15): 208-20.

    Girdin-mediated interactions between cadherin and the actin cytoskeleton are required for epithelial morphogenesis in Drosophila.
    Laprise P
    Development (Cambridge, England) 142.10 (2015 May 15): 1777-84.

    Modulation of dADAR-dependent RNA editing by the Drosophila fragile X mental retardation protein.
    Jongens TA
    Nature neuroscience 14.12 (2011 Oct 30): 1517-24.

    Distinct Rap1 activity states control the extent of epithelial invagination via α-catenin.
    Wieschaus EF
    Developmental cell 25.3 (2013 May 13): 299-309.

    A genome-wide screen identifies conserved protein hubs required for cadherin-mediated cell-cell adhesion.
    Nelson WJ
    The Journal of cell biology 204.2 (2014 Jan 20): 265-79.

    Dynamic microtubules produce an asymmetric E-cadherin-Bazooka complex to maintain segment boundaries.
    Brown NH
    The Journal of cell biology 201.6 (2013 Jun 10): 887-901.

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