DCAT-1

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$40.00
SKU: DCAT-1
View product citations for antibody DCAT-1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: catenin, alpha- (Drosophila)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Takeichi, M.
Isotype: RIgG2a, kappa light chain
Antigen Sequence:
Host Species: rat
Depositors Institution: RIKEN Center for Developmental Biology
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: Predicted: 102 kDa; Apparent: 110 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: alpha-Cat
Immunogen: Recombinant Drosophila alpha-catenin fused with the T7 phage gene 10 protein.
Alternate Gene Names: CG17947, ?cat, D?-cat, a-cat, ?-catenin
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3U1
Epitope Location or Sequence: aa 240-910
Uniprot ID: P35220 
Immunogen Sequence: Partial protein
Entrez Gene ID: 40517 
Additional Characterization:
Antibody Registry ID: AB_532377 
Additional Information:
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DCAT-1 was deposited to the DSHB by Takeichi, M. (DSHB Hybridoma Product DCAT-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

16 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication

    The Journal of cell biology
    Takeichi M
    The Journal of cell biology 121.5 (1993 Jun): 1133-40.

    IF References

    The Journal of cell biology
    Takeichi M
    The Journal of cell biology 121.5 (1993 Jun): 1133-40.

    Development genes and evolution
    Tsukita S
    Development genes and evolution 209.4 (1999 Apr): 218-25.

    Developmental biology
    Takeichi M
    Developmental biology 165.2 (1994 Oct): 716-26.

    Developmental biology
    Tepass U
    Developmental biology 177.1 (1996 Jul 10): 217-25.

    Journal of cell science
    Tepass U
    Journal of cell science 125.Pt 1 (2012 Jan 1): 233-45.

    Mechanisms of development
    Yamamoto D
    Mechanisms of development 78.1-2 (1998 Nov): 97-111.

    Genes to cells : devoted to molecular & cellular mechanisms
    Takeichi M
    Genes to cells : devoted to molecular & cellular mechanisms 2.1 (1997 Jan): 29-40.

    PLoS pathogens
    Fukatsu T
    PLoS pathogens 10.2 (2014 Feb): e1003956.

    Developmental biology
    Wehrli M
    Developmental biology 320.1 (2008 Aug 1): 226-41.

    Development (Cambridge, England)
    Chen J
    Development (Cambridge, England) 139.19 (2012 Oct): 3561-71.

    Developmental biology
    Großhans J
    Developmental biology 390.2 (2014 Jun 15): 208-20.

    Development (Cambridge, England)
    Laprise P
    Development (Cambridge, England) 142.10 (2015 May 15): 1777-84.

    Nature neuroscience
    Jongens TA
    Nature neuroscience 14.12 (2011 Oct 30): 1517-24.

    WB References

    The Journal of cell biology
    Takeichi M
    The Journal of cell biology 121.5 (1993 Jun): 1133-40.

    Development genes and evolution
    Tsukita S
    Development genes and evolution 209.4 (1999 Apr): 218-25.

    Developmental biology
    Takeichi M
    Developmental biology 165.2 (1994 Oct): 716-26.

    Journal of cell science
    Tepass U
    Journal of cell science 125.Pt 1 (2012 Jan 1): 233-45.

    Developmental cell
    Wieschaus EF
    Developmental cell 25.3 (2013 May 13): 299-309.

    Developmental biology
    Wehrli M
    Developmental biology 320.1 (2008 Aug 1): 226-41.

    The Journal of cell biology
    Nelson WJ
    The Journal of cell biology 204.2 (2014 Jan 20): 265-79.

    Developmental biology
    Großhans J
    Developmental biology 390.2 (2014 Jun 15): 208-20.

    Development (Cambridge, England)
    Laprise P
    Development (Cambridge, England) 142.10 (2015 May 15): 1777-84.

    Nature neuroscience
    Jongens TA
    Nature neuroscience 14.12 (2011 Oct 30): 1517-24.

    The Journal of cell biology
    Brown NH
    The Journal of cell biology 201.6 (2013 Jun 10): 887-901.

    IP References

    The Journal of cell biology
    Takeichi M
    The Journal of cell biology 121.5 (1993 Jun): 1133-40.

    Developmental biology
    Takeichi M
    Developmental biology 165.2 (1994 Oct): 716-26.

    Epitope Map References

    The Journal of cell biology
    Takeichi M
    The Journal of cell biology 121.5 (1993 Jun): 1133-40.

    All References

    The Journal of cell biology
    Takeichi M
    The Journal of cell biology 121.5 (1993 Jun): 1133-40.

    Development genes and evolution
    Tsukita S
    Development genes and evolution 209.4 (1999 Apr): 218-25.

    Developmental biology
    Takeichi M
    Developmental biology 165.2 (1994 Oct): 716-26.

    Developmental biology
    Tepass U
    Developmental biology 177.1 (1996 Jul 10): 217-25.

    Journal of cell science
    Tepass U
    Journal of cell science 125.Pt 1 (2012 Jan 1): 233-45.

    Mechanisms of development
    Yamamoto D
    Mechanisms of development 78.1-2 (1998 Nov): 97-111.

    Genes to cells : devoted to molecular & cellular mechanisms
    Takeichi M
    Genes to cells : devoted to molecular & cellular mechanisms 2.1 (1997 Jan): 29-40.

    PLoS pathogens
    Fukatsu T
    PLoS pathogens 10.2 (2014 Feb): e1003956.

    Developmental biology
    Wehrli M
    Developmental biology 320.1 (2008 Aug 1): 226-41.

    Development (Cambridge, England)
    Chen J
    Development (Cambridge, England) 139.19 (2012 Oct): 3561-71.

    Developmental biology
    Großhans J
    Developmental biology 390.2 (2014 Jun 15): 208-20.

    Development (Cambridge, England)
    Laprise P
    Development (Cambridge, England) 142.10 (2015 May 15): 1777-84.

    Nature neuroscience
    Jongens TA
    Nature neuroscience 14.12 (2011 Oct 30): 1517-24.

    Developmental cell
    Wieschaus EF
    Developmental cell 25.3 (2013 May 13): 299-309.

    The Journal of cell biology
    Nelson WJ
    The Journal of cell biology 204.2 (2014 Jan 20): 265-79.

    The Journal of cell biology
    Brown NH
    The Journal of cell biology 201.6 (2013 Jun 10): 887-901.

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