sn 7C

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$40.00
SKU: sn 7C
View product citations for antibody sn 7C on CiteAb

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Available: 143

DSHB Data Sheet

Catalog Fields

Antigen: singed (fascin)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Cooley, L.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Yale Medical School
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Use 1:20 in Blotto-Tween (PBS, 5% dry milk, 0.2% Tween) for Westerns or undiluted for ovary staining. (Cant et al., 1994). This antibody doesn't appear to work for immunoprecipitation or purification.
Antigen Molecular Weight: Predicted: 57 kDa; Apparent: 57 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: sn
Immunogen: Recombinant GST-singed fusion protein
Alternate Gene Names: CG1536
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain:
Epitope Location or Sequence:
Uniprot ID: Q24524 
Immunogen Sequence: Full length protein
Entrez Gene ID: 31717 
Additional Characterization:  
Antibody Registry ID: AB_528239 
Additional Information:
Recommended Applications: ELISA, Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
sn 7C was deposited to the DSHB by Cooley, L. (DSHB Hybridoma Product sn 7C)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

21 References

  • Initial Publication
  • IF References
  • WB References
  • ELISA References
  • All References
  • Initial Publication
    IF References

    Drosophila singed, a fascin homolog, is required for actin bundle formation during oogenesis and bristle extension.
    Cooley L
    The Journal of cell biology 125.2 (1994 Apr): 369-80.

    Interdependence of macrophage migration and ventral nerve cord development in Drosophila embryos.
    Wood W
    Development (Cambridge, England) 137.10 (2010 May): 1625-33.

    Antitumor effects of B3-PE and B3-LysPE40 in a nude mouse model of human breast cancer and the evaluation of B3-PE toxicity in monkeys.
    Pastan I
    Cancer research 52.11 (1992 Jun 1): 3189-93.

    Analysis of cell migration using Drosophila as a model system.
    Montell DJ
    Methods in molecular biology (Clifton, N.J.) 294. (2005): 175-202.

    Dynamic myosin activation promotes collective morphology and migration by locally balancing oppositional forces from surrounding tissue.
    McDonald JA
    Molecular biology of the cell 27.12 (2016 Jun 15): 1898-910.

    Mechanical feedback through E-cadherin promotes direction sensing during collective cell migration.
    Montell DJ
    Cell 157.5 (2014 May 22): 1146-59.

    Requirement for JAK/STAT signaling throughout border cell migration in Drosophila.
    Montell DJ
    Development (Cambridge, England) 132.15 (2005 Aug): 3483-92.

    Spatially localized Kuzbanian required for specific activation of Notch during border cell migration.
    Montell D
    Developmental biology 301.2 (2007 Jan 15): 532-40.

    Requirement for Par-6 and Bazooka in Drosophila border cell migration.
    Montell DJ
    Development (Cambridge, England) 131.21 (2004 Nov): 5243-51.

    On the role of PDZ domain-encoding genes in Drosophila border cell migration.
    McDonald JA
    G3 (Bethesda, Md.) 2.11 (2012 Nov): 1379-91.

    Analysis of cell migration using whole-genome expression profiling of migratory cells in the Drosophila ovary.
    Montell DJ
    Developmental cell 10.4 (2006 Apr): 483-95.

    Physiological apoptosis of polar cells during Drosophila oogenesis is mediated by Hid-dependent regulation of Diap1.
    Pret AM
    Cell death and differentiation 18.5 (2011 May): 793-805.

    Conserved mechanisms of tumorigenesis in the Drosophila adult midgut.
    Casali A
    PloS one 9.2 (2014): e88413.

    Border-cell migration requires integration of spatial and temporal signals by the BTB protein Abrupt.
    Montell D
    Nature cell biology 11.5 (2009 May): 569-79.

    Single amino acid mutations in Drosophila fascin disrupt actin bundling function in vivo.
    Cooley L
    Genetics 143.1 (1996 May): 249-58.

    WB References

    Drosophila singed, a fascin homolog, is required for actin bundle formation during oogenesis and bristle extension.
    Cooley L
    The Journal of cell biology 125.2 (1994 Apr): 369-80.

    Drosophila Fascin is a novel downstream target of prostaglandin signaling during actin remodeling.
    Tootle TL
    Molecular biology of the cell 23.23 (2012 Dec): 4567-78.

    Fascin promotes filopodia formation independent of its role in actin bundling.
    Stramer B
    The Journal of cell biology 197.4 (2012 May 14): 477-86.

    Molecular requirements for actin-based lamella formation in Drosophila S2 cells.
    Vale RD
    The Journal of cell biology 162.6 (2003 Sep 15): 1079-88.

    Fascin for cell migration in Drosophila.
    Plaza S
    Fly 3.4 (2009 Oct-Dec): 281-2.

    Single amino acid mutations in Drosophila fascin disrupt actin bundling function in vivo.
    Cooley L
    Genetics 143.1 (1996 May): 249-58.

    Drosophila fascin mutants are rescued by overexpression of the villin-like protein, quail.
    Cooley L
    Journal of cell science 111 ( Pt 2). (1998 Jan): 213-21.

    ELISA References
    All References

    Drosophila singed, a fascin homolog, is required for actin bundle formation during oogenesis and bristle extension.
    Cooley L
    The Journal of cell biology 125.2 (1994 Apr): 369-80.

    Interdependence of macrophage migration and ventral nerve cord development in Drosophila embryos.
    Wood W
    Development (Cambridge, England) 137.10 (2010 May): 1625-33.

    Antitumor effects of B3-PE and B3-LysPE40 in a nude mouse model of human breast cancer and the evaluation of B3-PE toxicity in monkeys.
    Pastan I
    Cancer research 52.11 (1992 Jun 1): 3189-93.

    Analysis of cell migration using Drosophila as a model system.
    Montell DJ
    Methods in molecular biology (Clifton, N.J.) 294. (2005): 175-202.

    Dynamic myosin activation promotes collective morphology and migration by locally balancing oppositional forces from surrounding tissue.
    McDonald JA
    Molecular biology of the cell 27.12 (2016 Jun 15): 1898-910.

    Mechanical feedback through E-cadherin promotes direction sensing during collective cell migration.
    Montell DJ
    Cell 157.5 (2014 May 22): 1146-59.

    Requirement for JAK/STAT signaling throughout border cell migration in Drosophila.
    Montell DJ
    Development (Cambridge, England) 132.15 (2005 Aug): 3483-92.

    Spatially localized Kuzbanian required for specific activation of Notch during border cell migration.
    Montell D
    Developmental biology 301.2 (2007 Jan 15): 532-40.

    Requirement for Par-6 and Bazooka in Drosophila border cell migration.
    Montell DJ
    Development (Cambridge, England) 131.21 (2004 Nov): 5243-51.

    On the role of PDZ domain-encoding genes in Drosophila border cell migration.
    McDonald JA
    G3 (Bethesda, Md.) 2.11 (2012 Nov): 1379-91.

    Analysis of cell migration using whole-genome expression profiling of migratory cells in the Drosophila ovary.
    Montell DJ
    Developmental cell 10.4 (2006 Apr): 483-95.

    Physiological apoptosis of polar cells during Drosophila oogenesis is mediated by Hid-dependent regulation of Diap1.
    Pret AM
    Cell death and differentiation 18.5 (2011 May): 793-805.

    Conserved mechanisms of tumorigenesis in the Drosophila adult midgut.
    Casali A
    PloS one 9.2 (2014): e88413.

    Border-cell migration requires integration of spatial and temporal signals by the BTB protein Abrupt.
    Montell D
    Nature cell biology 11.5 (2009 May): 569-79.

    Single amino acid mutations in Drosophila fascin disrupt actin bundling function in vivo.
    Cooley L
    Genetics 143.1 (1996 May): 249-58.

    Drosophila Fascin is a novel downstream target of prostaglandin signaling during actin remodeling.
    Tootle TL
    Molecular biology of the cell 23.23 (2012 Dec): 4567-78.

    Fascin promotes filopodia formation independent of its role in actin bundling.
    Stramer B
    The Journal of cell biology 197.4 (2012 May 14): 477-86.

    Molecular requirements for actin-based lamella formation in Drosophila S2 cells.
    Vale RD
    The Journal of cell biology 162.6 (2003 Sep 15): 1079-88.

    Fascin for cell migration in Drosophila.
    Plaza S
    Fly 3.4 (2009 Oct-Dec): 281-2.

    Drosophila fascin mutants are rescued by overexpression of the villin-like protein, quail.
    Cooley L
    Journal of cell science 111 ( Pt 2). (1998 Jan): 213-21.

    Genetic ablation of Drosophila phagocytes reveals their contribution to both development and resistance to bacterial infection.
    Leulier F
    Journal of innate immunity 1.4 (2009): 322-34.

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