sn 7C
Catalog Fields
Clone ID/Product Name: sn 7C
Available to For-Profits: No
Alternate Antibody Name:
Gene Symbol: sn
Ab Isotype: MIgG1, kappa light chain
Gene Name:
Entrez Gene ID: 31717
Clonality: Monoclonal
Immunogen: Recombinant GST-singed fusion protein
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain:
Epitope Mapped: No
Antigen Name: singed (fascin)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 7/12/2001
Antigen Molecular Weight: Predicted: 57 kDa; Apparent: 57 kDa
Depositor: Cooley, L.
Antigen Sequence:
Depositor Institution: Yale Medical School
Antigen Species: Drosophila
Depositor Notes: Use 1:20 in Blotto-Tween (PBS, 5% dry milk, 0.2% Tween) for Westerns or undiluted for ovary staining. (Cant et al., 1994). This antibody doesn't appear to work for immunoprecipitation or purification.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Drosophila
Additional Information:
Recommended Applications: ELISA, Immunofluorescence, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
sn 7C was deposited to the DSHB by Cooley, L. (DSHB Hybridoma Product sn 7C)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
22 References
Initial Publication
Drosophila singed, a fascin homolog, is required for actin bundle formation during oogenesis and bristle extension.
Cooley L
The Journal of cell biology 125.2 (1994 Apr): 369-80.