Arl8

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$45.00
SKU: Arl8-p
View product citations for antibody Arl8 on CiteAb

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Available: 39

DSHB Data Sheet

Catalog Fields

Antigen: ADP-ribosylation factor-like protein 8
Available to For-Profits: Yes
Antigen Species: Drosophila
Hybridoma Cells Available (Non-Profit): No
Isotype: Rabbit IgG
Depositor: Munro, S.
Host Species: rabbit
Antigen Sequence:
Positive Tested Species Reactivity: Drosophila
Depositors Institution: MRC Laboratory of Molecular Biology
Antigen Molecular Weight: 21.3 kDa
Depositors Notes: 4% paraformaldehye fixation. The recommended dilution for IF is 1:200 and 1:1000 for WB.
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Recombinant GST-fusion
Gene: Gie
Alternate Antibody Name:
Alternate Gene Names: Arl8, Dmel_CG7891
Alternate Antigen Name:
Clonality: Polyclonal
Myeloma Strain:
Epitope Mapped: No
Uniprot ID: Q9VHV5 
Epitope Location or Sequence:
Entrez Gene ID: 40961 
Immunogen Sequence: amino acids 18-186 of Drosophila Arl8 (CG7891)
Antibody Registry ID: AB_2618258 
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
Additional Information: FlyBase: FBgn0037551. This antigen is also known as GTPase indispensable for equal segregation of chromosomes. RRID:AB_2618258
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Arl8 was deposited to the DSHB by Munro, S. (DSHB Hybridoma Product Arl8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

3 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication

    An N-terminally acetylated Arf-like GTPase is localised to lysosomes and affects their motility.
    Munro S
    Journal of cell science 119.Pt 8 (2006 Apr 15): 1494-503.

    IF References

    An N-terminally acetylated Arf-like GTPase is localised to lysosomes and affects their motility.
    Munro S
    Journal of cell science 119.Pt 8 (2006 Apr 15): 1494-503.

    Cellular homeostasis in the Drosophila retina requires the lipid phosphatase Sac1.
    Brill JA
    Molecular biology of the cell 31.11 (2020 May 15): 1183-1199.

    WB References

    An N-terminally acetylated Arf-like GTPase is localised to lysosomes and affects their motility.
    Munro S
    Journal of cell science 119.Pt 8 (2006 Apr 15): 1494-503.

    IHC References
    All References

    α-synuclein impairs autophagosome maturation through abnormal actin stabilization.
    Feany MB
    PLoS genetics 17.2 (2021 Feb): e1009359.

    An N-terminally acetylated Arf-like GTPase is localised to lysosomes and affects their motility.
    Munro S
    Journal of cell science 119.Pt 8 (2006 Apr 15): 1494-503.

    Cellular homeostasis in the Drosophila retina requires the lipid phosphatase Sac1.
    Brill JA
    Molecular biology of the cell 31.11 (2020 May 15): 1183-1199.

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