Hrs 27-4

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$40.00
SKU: Hrs 27-4
View product citations for antibody Hrs 27-4 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Hepatocyte growth factor-regulated tyrosine kinase substrate
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Munro, S.
Isotype: MIgG2a, kappa light chain
Depositors Institution: MRC Laboratory of Molecular Biology
Host Species: mouse
Depositors Notes: 4% paraformaldehye fixation/ Antigen retrieval not required
Positive Tested Species Reactivity: Drosophila
Gene: Hrs
Antigen Molecular Weight: 72.44 kDa
Alternate Gene Names: Dmel_CG2903, Vps27
Immunogen: Hrs-N-6xHis
Clonality: Monoclonal
Myeloma Strain: SP2/0-Ag14
Epitope Mapped: No
Uniprot ID: Q960X8 
Immunogen Sequence: amino acids 2-647 of Genbank accession NP_722831.1
Entrez Gene ID: 33458 
Additional Information: Flybase: FBgn0031450. RRID:AB_2618261
Antibody Registry ID: AB_2618261 
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Hrs 27-4 was deposited to the DSHB by Munro, S. (DSHB Hybridoma Product Hrs 27-4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication
    IF References

    Hsp90 Mediates Membrane Deformation and Exosome Release.
    Verstreken P
    Molecular cell 71.5 (2018 Sep 6): 689-702.e9.

    WB References

    Hsp90 Mediates Membrane Deformation and Exosome Release.
    Verstreken P
    Molecular cell 71.5 (2018 Sep 6): 689-702.e9.

    All References

    Hsp90 Mediates Membrane Deformation and Exosome Release.
    Verstreken P
    Molecular cell 71.5 (2018 Sep 6): 689-702.e9.

    An antibody toolkit for the study of membrane traffic in Drosophila melanogaster.
    Munro S
    Biology open 5.7 (2016 Jul 15): 987-92.

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