Cnx99A 6-2-1

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SKU: Cnx99A 6-2-1
View product citations for antibody Cnx99A 6-2-1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Calnexin 99A
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Munro, S.
Isotype: MIgG1, kappa light chain
Depositors Institution: MRC Laboratory of Molecular Biology
Host Species: mouse
Depositors Notes: 4% paraformaldehye fixation/ Antigen retrieval not required; may cross-react with Cnx14D and CG1924
Positive Tested Species Reactivity: Drosophila
Gene: Cnx99A
Antigen Molecular Weight: 75.94 kDa
Alternate Gene Names: Dmel_CG11958
Immunogen: Recombinant Calnexin99A-N-6XHis
Clonality: Monoclonal
Myeloma Strain: SP2/0-Ag14
Epitope Mapped: No
Uniprot ID: Q9VAL7 
Immunogen Sequence: amino acids 73-677 of genbank accession AAO39490.1
Entrez Gene ID: 44643 
Additional Information: Flybase: FBgn0015622. RRID:AB_2722011
Antibody Registry ID: AB_2722011 
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Cnx99A 6-2-1 was deposited to the DSHB by Munro, S. (DSHB Hybridoma Product Cnx99A 6-2-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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