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View product citations for antibody GMAP on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Golgi microtubule-associated protein
Available to For-Profits: Yes
Antigen Species: Drosophila
Hybridoma Cells Available (Non-Profit): No
Isotype: Goat IgG
Depositor: Munro, S.
Host Species: goat
Antigen Sequence:
Positive Tested Species Reactivity: Drosophila
Depositors Institution: MRC Laboratory of Molecular Biology
Antigen Molecular Weight: 158.55 kDa
Depositors Notes: 4% paraformaldehye fixation/ Antigen retrieval not required. The recommended dilution for IF is 1:2000 and for WB is 1:1000.
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: GMAP-N-6xHis-MBP
Gene: Gmap
Alternate Antibody Name:
Alternate Gene Names: Dmel_CG33206
Alternate Antigen Name:
Clonality: Polyclonal
Myeloma Strain:
Epitope Mapped: No
Uniprot ID: Q9VXU2 
Epitope Location or Sequence:
Entrez Gene ID: 32483 
Immunogen Sequence: amino acids 778-1057 of Genbank accession NP_788907.1
Antibody Registry ID: AB_2618259 
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Additional Information: FLyBase: FBgn0027287. RRID:AB_2618259
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
GMAP was deposited to the DSHB by Munro, S. (DSHB Hybridoma Product GMAP)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

2 References

  • Initial Publication
  • IHC References
  • All References
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