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Available: 43

DSHB Data Sheet

Catalog Fields

Antigen: Golgi microtubule-associated protein
Hybridoma Cells Available:
Antigen Species: Drosophila
Depositor: Munro, S.
Isotype: Goat IgG
Antigen Sequence:
Host Species: goat
Depositors Institution: MRC Laboratory of Molecular Biology
Positive Tested Species Reactivity: Drosophila
Depositors Notes: 4% paraformaldehye fixation/ Antigen retrieval not required. The recommended dilution for IF is 1:2000 and for WB is 1:1000.
Antigen Molecular Weight: 158.55 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Gmap
Immunogen: GMAP-N-6xHis-MBP
Alternate Gene Names: Dmel_CG33206
Alternate Antibody Name:
Clonality: Polyclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain:
Epitope Location or Sequence:
Uniprot ID: Q9VXU2 
Immunogen Sequence: amino acids 778-1057 of Genbank accession NP_788907.1
Entrez Gene ID: 32483 
Additional Characterization:
Antibody Registry ID: AB_2618259 
Additional Information: FLyBase: FBgn0027287. RRID:AB_2618259
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
GMAP was deposited to the DSHB by Munro, S. (DSHB Hybridoma Product GMAP)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IHC References
  • All References
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