GMAP

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$40.00
SKU: GMAP-p
View product citations for antibody GMAP on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Golgi microtubule-associated protein
Hybridoma Cells Available: No
Antigen Species: Drosophila
Depositor: Munro, S.
Isotype: Goat IgG
Antigen Sequence:
Host Species: goat
Depositors Institution: MRC Laboratory of Molecular Biology
Positive Tested Species Reactivity: Drosophila
Depositors Notes: 4% paraformaldehye fixation/ Antigen retrieval not required. The recommended dilution for IF is 1:2000 and for WB is 1:1000.
Antigen Molecular Weight: 158.55 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Gmap
Immunogen: GMAP-N-6xHis-MBP
Alternate Gene Names: Dmel_CG33206
Alternate Antibody Name:
Clonality: Polyclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain:
Epitope Location or Sequence:
Uniprot ID: Q9VXU2 
Immunogen Sequence: amino acids 778-1057 of Genbank accession NP_788907.1
Entrez Gene ID: 32483 
Additional Characterization:  
Antibody Registry ID: AB_2618259 
Additional Information: FLyBase: FBgn0027287. RRID:AB_2618259
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
GMAP was deposited to the DSHB by Munro, S. (DSHB Hybridoma Product GMAP)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IHC References
  • All References
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