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SKU: Golgin245-p
View product citations for antibody Golgin245 on CiteAb

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Available: 35

DSHB Data Sheet

Catalog Fields

Antigen: Golgin-245 ortholog
Hybridoma Cells Available: No
Antigen Species: Drosophila
Depositor: Munro, S.
Isotype: Goat IgG
Depositors Institution: MRC Laboratory of Molecular Biology
Host Species: goat
Depositors Notes: 4% paraformaldehye fixation/ Antigen retrieval not required
Positive Tested Species Reactivity: Drosophila
Gene: Golgin245
Antigen Molecular Weight: 170.20 kDa
Alternate Gene Names: Dmel_CG3493
Immunogen: Golgin245-N-6xHis-MBP
Clonality: Polyclonal
Uniprot ID: Q9W1R3 
Epitope Mapped: No
Entrez Gene ID: 37702 
Immunogen Sequence: amino acids 1-340 of Genbank accession AAL68382.1
Antibody Registry ID: AB_2569587 
Additional Information: FlyBase: FBgn0034854. RRID:AB_2569587
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Golgin245 was deposited to the DSHB by Munro, S. (DSHB Hybridoma Product Golgin245)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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