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SKU: Golgin245-p
View product citations for antibody Golgin245 on CiteAb

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Available: 18

DSHB Data Sheet

Catalog Fields

Product Name/ID: Golgin245
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: Golgin245
Ab Isotype: Goat IgG
Gene Symbol:
Antibody Registry ID: AB_2569587 
Uniprot ID: Q9W1R3 
Entrez Gene ID: 37702 
Clonality: Polyclonal
Immunogen: Golgin245-N-6xHis-MBP
Immunogen Sequence: amino acids 1-340 of Genbank accession AAL68382.1
Myeloma Strain:
Epitope Mapped: No
Antigen Name: Golgin-245 ortholog
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 5/26/2016
Antigen Molecular Weight: 170.20 kDa
Depositor: Munro, S.
Antigen Sequence:
Depositor Institution: MRC Laboratory of Molecular Biology
Antigen Species: Drosophila
Depositor Notes: 4% paraformaldehye fixation/ Antigen retrieval not required
Host Species: goat
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Drosophila
Additional Information: FlyBase: FBgn0034854. RRID:AB_2569587
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Golgin245 was deposited to the DSHB by Munro, S. (DSHB Hybridoma Product Golgin245)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

2 References

  • Initial Publication
  • IF References
  • All References
  • Initial Publication
    IF References

    Sec71 separates Golgi stacks in Drosophila S2 cells.
    Satoh AK
    Journal of cell science 133.24 (2020 Dec 29): .

    All References

    Sec71 separates Golgi stacks in Drosophila S2 cells.
    Satoh AK
    Journal of cell science 133.24 (2020 Dec 29): .

    An antibody toolkit for the study of membrane traffic in Drosophila melanogaster.
    Munro S
    Biology open 5.7 (2016 Jul 15): 987-92.

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