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SKU: Golgin245-p
View product citations for antibody Golgin245 on CiteAb

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Available: 50

DSHB Data Sheet

Catalog Fields

Antigen: Golgin-245 ortholog
Hybridoma Cells Available: No
Antigen Species: Drosophila
Depositor: Munro, S.
Isotype: Goat IgG
Antigen Sequence:
Host Species: goat
Depositors Institution: MRC Laboratory of Molecular Biology
Positive Tested Species Reactivity: Drosophila
Depositors Notes: 4% paraformaldehye fixation/ Antigen retrieval not required
Antigen Molecular Weight: 170.20 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Golgin245
Immunogen: Golgin245-N-6xHis-MBP
Alternate Gene Names: Dmel_CG3493
Alternate Antibody Name:
Clonality: Polyclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain:
Epitope Location or Sequence:
Uniprot ID: Q9W1R3 
Immunogen Sequence: amino acids 1-340 of Genbank accession AAL68382.1
Entrez Gene ID: 37702 
Additional Characterization:  
Antibody Registry ID: AB_2569587 
Additional Information: FlyBase: FBgn0034854. RRID:AB_2569587
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Golgin245 was deposited to the DSHB by Munro, S. (DSHB Hybridoma Product Golgin245)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IF References
  • All References
  • Initial Publication
    IF References

    Sec71 separates Golgi stacks in <i>Drosophila</i> S2 cells.
    Satoh AK
    Journal of cell science 133.24 (2020 Dec 29): .

    All References

    Sec71 separates Golgi stacks in <i>Drosophila</i> S2 cells.
    Satoh AK
    Journal of cell science 133.24 (2020 Dec 29): .

    An antibody toolkit for the study of membrane traffic in Drosophila melanogaster.
    Munro S
    Biology open 5.7 (2016 Jul 15): 987-92.

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