Rab7

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$40.00
SKU: Rab7
View product citations for antibody Rab7 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: RAb7/CG5915 protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Munro, S.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: MRC Laboratory of Molecular Biology
Positive Tested Species Reactivity: Drosophila
Depositors Notes: 4% paraformaldehye fixation/ Antigen retrieval not required
Antigen Molecular Weight: 23.34 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Rab7
Immunogen: Peptide conjugated to bacterial transglutaminase (BTG)
Alternate Gene Names: Dmel_CG5915
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0-Ag14
Epitope Location or Sequence:
Uniprot ID: O76742 
Immunogen Sequence: amino acids 184-200 of Genbank accession NP_524472.1
Entrez Gene ID: 42841 
Additional Characterization:  
Antibody Registry ID: AB_2722471 
Additional Information: FlyBase: FBgn0015795. RRID:AB_2722471
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Rab7 was deposited to the DSHB by Munro, S. (DSHB Hybridoma Product Rab7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

3 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Initial Publication
    IF References

    Cellular homeostasis in the <i>Drosophila</i> retina requires the lipid phosphatase Sac1.
    Brill JA
    Molecular biology of the cell 31.11 (2020 May 15): 1183-1199.

    IHC References
    All References

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