Rab7

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SKU: Rab7

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DSHB Data Sheet

Catalog Fields

Product Name/ID: Rab7
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: Rab7
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_2722471 
Uniprot ID: O76742 
RRID:  
Entrez Gene ID: 42841 
Clonality: Monoclonal
Immunogen: Peptide conjugated to bacterial transglutaminase (BTG)
Clone:
Immunogen Sequence: amino acids 184-200 of Genbank accession NP_524472.1
Myeloma Strain: SP2/0-Ag14
Epitope Mapped: Yes
Antigen Name: RAb7/CG5915 protein
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 5/26/2016
Antigen Molecular Weight: 23.34 kDa
Depositor: Munro, S.
Antigen Sequence:
Depositor Institution: MRC Laboratory of Molecular Biology
Antigen Species: Drosophila
Depositor Notes: 4% paraformaldehye fixation/ Antigen retrieval not required
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: FlyBase: FBgn0015795. RRID:AB_2722471
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Rab7 was deposited to the DSHB by Munro, S. (DSHB Hybridoma Product Rab7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

3 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Initial Publication
    IF References

    Cellular homeostasis in the Drosophila retina requires the lipid phosphatase Sac1.
    Brill JA
    Molecular biology of the cell 31.11 (2020 May 15): 1183-1199.

    IHC References
    All References

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