Rab7

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$40.00
SKU: Rab7
View product citations for antibody Rab7 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: RAb7/CG5915 protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Munro, S.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: MRC Laboratory of Molecular Biology
Positive Tested Species Reactivity: Drosophila
Depositors Notes: 4% paraformaldehye fixation/ Antigen retrieval not required
Antigen Molecular Weight: 23.34 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Rab7
Immunogen: Peptide conjugated to bacterial transglutaminase (BTG)
Alternate Gene Names: Dmel_CG5915
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0-Ag14
Epitope Location or Sequence:
Uniprot ID: O76742 
Immunogen Sequence: amino acids 184-200 of Genbank accession NP_524472.1
Entrez Gene ID: 42841 
Additional Characterization:
Antibody Registry ID: AB_2722471 
Additional Information: FlyBase: FBgn0015795. RRID:AB_2722471
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Rab7 was deposited to the DSHB by Munro, S. (DSHB Hybridoma Product Rab7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IHC References
  • All References
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