CCD6B-1

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$40.00
SKU: CCD6B-1
View product citations for antibody CCD6B-1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: cadherin-6
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Takeichi, M. / Nakagawa, S.
Isotype: MIgG1, lambda light chain
Depositors Institution: Faculty of Science, Kyoto University
Host Species: mouse
Depositors Notes: Stains dorsal neural tube/premigratory neural crest cells
Positive Tested Species Reactivity: Chicken, Lizard, Quail
Gene: CDH6
Antigen Molecular Weight: Predicted: 82 kDa; Apparent: 120 kDa
Alternate Gene Names: c-cad6B, cad6B
Immunogen: Recombinant chicken cadherin-7 (N-terminal 597 aa) fused to human Fc; expressed in COS-7 cells
Clonality: Monoclonal
Alternate Antibody Name: CC6B-1
Epitope Mapped: Yes
Myeloma Strain: P3U1
Epitope Location or Sequence: extracellular domain
Uniprot ID: Q90762 
Immunogen Sequence: Partial protein
Entrez Gene ID: 414842 
Antibody Registry ID: AB_531766 
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CCD6B-1 was deposited to the DSHB by Takeichi, M. / Nakagawa, S. (DSHB Hybridoma Product CCD6B-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

10 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication

    Neural crest emigration from the neural tube depends on regulated cadherin expression.
    Takeichi M
    Development (Cambridge, England) 125.15 (1998 Aug): 2963-71.

    IF References

    Neural crest emigration from the neural tube depends on regulated cadherin expression.
    Takeichi M
    Development (Cambridge, England) 125.15 (1998 Aug): 2963-71.

    Molecular profiling indicates avian branchiomotor nuclei invade the hindbrain alar plate.
    Redies C
    Neuroscience 128.4 (2004): 785-96.

    Neural crest emigration from the neural tube depends on regulated cadherin expression.
    Takeichi M
    Development (Cambridge, England) 125.15 (1998 Aug): 2963-71.

    Cadherin expression in the developing chicken cochlea.
    Redies C
    Developmental dynamics : an official publication of the American Association of Anatomists 236.8 (2007 Aug): 2331-7.

    Cadherins guide migrating Purkinje cells to specific parasagittal domains during cerebellar development.
    Redies C
    Molecular and cellular neurosciences 25.1 (2004 Jan): 138-52.

    Phenotype-dependent expression of cadherin 6B in vascular and visceral smooth muscle cells.
    Sobue K
    FEBS letters 469.1 (2000 Mar 3): 67-71.

    Sip1 mediates an E-cadherin-to-N-cadherin switch during cranial neural crest EMT.
    Bronner ME
    The Journal of cell biology 203.5 (2013 Dec 9): 835-47.

    Cadherin-6B undergoes macropinocytosis and clathrin-mediated endocytosis during cranial neural crest cell EMT.
    Taneyhill LA
    Journal of cell science 128.9 (2015 May 1): 1773-86.

    A critical role for Cadherin6B in regulating avian neural crest emigration.
    Bronner-Fraser M
    Developmental biology 312.2 (2007 Dec 15): 533-44.

    Differential expression of the ADAMs in developing chicken retina.
    Luo J
    Development, growth & differentiation 53.5 (2011 Jun): 726-39.

    Novel insight into the function and regulation of alphaN-catenin by Snail2 during chick neural crest cell migration.
    Taneyhill LA
    Developmental biology 344.2 (2010 Aug 15): 896-910.

    WB References
    IHC References
    Epitope Map References

    Neural crest emigration from the neural tube depends on regulated cadherin expression.
    Takeichi M
    Development (Cambridge, England) 125.15 (1998 Aug): 2963-71.

    All References

    Molecular profiling indicates avian branchiomotor nuclei invade the hindbrain alar plate.
    Redies C
    Neuroscience 128.4 (2004): 785-96.

    Neural crest emigration from the neural tube depends on regulated cadherin expression.
    Takeichi M
    Development (Cambridge, England) 125.15 (1998 Aug): 2963-71.

    Cadherin expression in the developing chicken cochlea.
    Redies C
    Developmental dynamics : an official publication of the American Association of Anatomists 236.8 (2007 Aug): 2331-7.

    Cadherins guide migrating Purkinje cells to specific parasagittal domains during cerebellar development.
    Redies C
    Molecular and cellular neurosciences 25.1 (2004 Jan): 138-52.

    Phenotype-dependent expression of cadherin 6B in vascular and visceral smooth muscle cells.
    Sobue K
    FEBS letters 469.1 (2000 Mar 3): 67-71.

    Sip1 mediates an E-cadherin-to-N-cadherin switch during cranial neural crest EMT.
    Bronner ME
    The Journal of cell biology 203.5 (2013 Dec 9): 835-47.

    Cadherin-6B undergoes macropinocytosis and clathrin-mediated endocytosis during cranial neural crest cell EMT.
    Taneyhill LA
    Journal of cell science 128.9 (2015 May 1): 1773-86.

    A critical role for Cadherin6B in regulating avian neural crest emigration.
    Bronner-Fraser M
    Developmental biology 312.2 (2007 Dec 15): 533-44.

    Differential expression of the ADAMs in developing chicken retina.
    Luo J
    Development, growth & differentiation 53.5 (2011 Jun): 726-39.

    Novel insight into the function and regulation of alphaN-catenin by Snail2 during chick neural crest cell migration.
    Taneyhill LA
    Developmental biology 344.2 (2010 Aug 15): 896-910.

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