CCD6B-1

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$45.00 to $425.00
SKU: CCD6B-1
View product citations for antibody CCD6B-1 on CiteAb

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Available: 168

DSHB Data Sheet

Catalog Fields

Antigen: cadherin-6
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Takeichi, M. / Nakagawa, S.
Isotype: MIgG1, lambda light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Kyoto University
Positive Tested Species Reactivity: Chicken, Lizard, Quail
Depositors Notes: Stains dorsal neural tube/premigratory neural crest cells
Antigen Molecular Weight: Predicted: 82 kDa; Apparent: 120 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: CDH6
Immunogen: Recombinant chicken cadherin-7 (N-terminal 597 aa) fused to human Fc; expressed in COS-7 cells
Alternate Gene Names: c-cad6B, cad6B
Alternate Antibody Name: CC6B-1
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3U1
Epitope Location or Sequence: extracellular domain
Uniprot ID: Q90762 
Immunogen Sequence: Partial protein
Entrez Gene ID: 414842 
Additional Characterization:  
Antibody Registry ID: AB_531766 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CCD6B-1 was deposited to the DSHB by Takeichi, M. / Nakagawa, S. (DSHB Hybridoma Product CCD6B-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

13 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication

    Neural crest emigration from the neural tube depends on regulated cadherin expression.
    Takeichi M
    Development (Cambridge, England) 125.15 (1998 Aug): 2963-71.

    IF References

    Neural crest emigration from the neural tube depends on regulated cadherin expression.
    Takeichi M
    Development (Cambridge, England) 125.15 (1998 Aug): 2963-71.

    Molecular profiling indicates avian branchiomotor nuclei invade the hindbrain alar plate.
    Redies C
    Neuroscience 128.4 (2004): 785-96.

    Neural crest emigration from the neural tube depends on regulated cadherin expression.
    Takeichi M
    Development (Cambridge, England) 125.15 (1998 Aug): 2963-71.

    Cadherin expression in the developing chicken cochlea.
    Redies C
    Developmental dynamics : an official publication of the American Association of Anatomists 236.8 (2007 Aug): 2331-7.

    Cadherins guide migrating Purkinje cells to specific parasagittal domains during cerebellar development.
    Redies C
    Molecular and cellular neurosciences 25.1 (2004 Jan): 138-52.

    Phenotype-dependent expression of cadherin 6B in vascular and visceral smooth muscle cells.
    Sobue K
    FEBS letters 469.1 (2000 Mar 3): 67-71.

    Sip1 mediates an E-cadherin-to-N-cadherin switch during cranial neural crest EMT.
    Bronner ME
    The Journal of cell biology 203.5 (2013 Dec 9): 835-47.

    Cadherin-6B undergoes macropinocytosis and clathrin-mediated endocytosis during cranial neural crest cell EMT.
    Taneyhill LA
    Journal of cell science 128.9 (2015 May 1): 1773-86.

    A critical role for Cadherin6B in regulating avian neural crest emigration.
    Bronner-Fraser M
    Developmental biology 312.2 (2007 Dec 15): 533-44.

    Differential expression of the ADAMs in developing chicken retina.
    Luo J
    Development, growth & differentiation 53.5 (2011 Jun): 726-39.

    Novel insight into the function and regulation of alphaN-catenin by Snail2 during chick neural crest cell migration.
    Taneyhill LA
    Developmental biology 344.2 (2010 Aug 15): 896-910.

    WB References
    IHC References
    Epitope Map References

    Neural crest emigration from the neural tube depends on regulated cadherin expression.
    Takeichi M
    Development (Cambridge, England) 125.15 (1998 Aug): 2963-71.

    All References

    Molecular profiling indicates avian branchiomotor nuclei invade the hindbrain alar plate.
    Redies C
    Neuroscience 128.4 (2004): 785-96.

    Sip1 mediates an E-cadherin-to-N-cadherin switch during cranial neural crest EMT.
    Bronner ME
    The Journal of cell biology 203.5 (2013 Dec 9): 835-47.

    Data on the effects of N-cadherin perturbation on the expression of type II cadherin proteins and major signaling pathways.
    Rogers CD
    Data in brief 20. (2018 Oct): 419-425.

    A catenin-dependent balance between N-cadherin and E-cadherin controls neuroectodermal cell fate choices.
    Bronner ME
    Mechanisms of development 152. (2018 Aug): 44-56.

    Essential function and targets of BMP signaling during midbrain neural crest delamination.
    Bronner ME
    Developmental biology 477. (2021 Sep): 251-261.

    Neural crest emigration from the neural tube depends on regulated cadherin expression.
    Takeichi M
    Development (Cambridge, England) 125.15 (1998 Aug): 2963-71.

    Cadherin expression in the developing chicken cochlea.
    Redies C
    Developmental dynamics : an official publication of the American Association of Anatomists 236.8 (2007 Aug): 2331-7.

    Cadherins guide migrating Purkinje cells to specific parasagittal domains during cerebellar development.
    Redies C
    Molecular and cellular neurosciences 25.1 (2004 Jan): 138-52.

    Phenotype-dependent expression of cadherin 6B in vascular and visceral smooth muscle cells.
    Sobue K
    FEBS letters 469.1 (2000 Mar 3): 67-71.

    Cadherin-6B undergoes macropinocytosis and clathrin-mediated endocytosis during cranial neural crest cell EMT.
    Taneyhill LA
    Journal of cell science 128.9 (2015 May 1): 1773-86.

    A critical role for Cadherin6B in regulating avian neural crest emigration.
    Bronner-Fraser M
    Developmental biology 312.2 (2007 Dec 15): 533-44.

    Differential expression of the ADAMs in developing chicken retina.
    Luo J
    Development, growth & differentiation 53.5 (2011 Jun): 726-39.

    Novel insight into the function and regulation of alphaN-catenin by Snail2 during chick neural crest cell migration.
    Taneyhill LA
    Developmental biology 344.2 (2010 Aug 15): 896-910.

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