CCD6B-1
Catalog Fields
Antigen: cadherin-6
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Takeichi, M. / Nakagawa, S.
Isotype: MIgG1, lambda light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Kyoto University
Positive Tested Species Reactivity: Chicken, Lizard, Quail
Depositors Notes: Stains dorsal neural tube/premigratory neural crest cells
Predicted Species Reactivity:
Gene: CDH6
Immunogen: Recombinant chicken cadherin-7 (N-terminal 597 aa) fused to human Fc; expressed in COS-7 cells
Alternate Gene Names: c-cad6B, cad6B
Alternate Antibody Name: CC6B-1
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3U1
Epitope Location or Sequence: extracellular domain
Uniprot ID: Q90762
Immunogen Sequence: Partial protein
Entrez Gene ID: 414842
Antibody Registry ID: AB_531766
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CCD6B-1 was deposited to the DSHB by Takeichi, M. / Nakagawa, S. (DSHB Hybridoma Product CCD6B-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
13 References
Initial Publication
Neural crest emigration from the neural tube depends on regulated cadherin expression.
Takeichi M
Development (Cambridge, England) 125.15 (1998 Aug): 2963-71.