CCD6B-1
Catalog Fields
Antigen: cadherin-6
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Takeichi, M. / Nakagawa, S.
Isotype: MIgG1, lambda light chain
Depositors Institution: Faculty of Science, Kyoto University
Host Species: mouse
Depositors Notes: Stains dorsal neural tube/premigratory neural crest cells
Positive Tested Species Reactivity: Chicken, Lizard, Quail
Gene: CDH6
Antigen Molecular Weight: Predicted: 82 kDa; Apparent: 120 kDa
Alternate Gene Names: c-cad6B, cad6B
Immunogen: Recombinant chicken cadherin-7 (N-terminal 597 aa) fused to human Fc; expressed in COS-7 cells
Clonality: Monoclonal
Alternate Antibody Name: CC6B-1
Epitope Mapped: Yes
Myeloma Strain: P3U1
Epitope Location or Sequence: extracellular domain
Uniprot ID: Q90762
Immunogen Sequence: Partial protein
Entrez Gene ID: 414842
Antibody Registry ID: AB_531766
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CCD6B-1 was deposited to the DSHB by Takeichi, M. / Nakagawa, S. (DSHB Hybridoma Product CCD6B-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
10 References
Initial Publication
Neural crest emigration from the neural tube depends on regulated cadherin expression.
Takeichi M
Development (Cambridge, England) 125.15 (1998 Aug): 2963-71.