CCD7-1

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$40.00
SKU: CCD7-1
View product citations for antibody CCD7-1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: cadherin-7
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Takeichi, M. / Nakagawa, S.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Faculty of Science, Kyoto University
Positive Tested Species Reactivity: Chicken, Lizard, Quail, Turtle
Depositors Notes:
Antigen Molecular Weight: Predicted: 82 kDa; Apparent: 105 kDa (additional lower MW band)
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: CDH7
Immunogen: Recombinant chicken cadherin-6 (N-terminal 605 aa) fused to human Fc; expressed in COS-7 cells
Alternate Gene Names: cad7
Alternate Antibody Name: NK2, CC7-1
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3U1
Epitope Location or Sequence: extracellular domain
Uniprot ID: Q90763 
Immunogen Sequence: Partial protein
Entrez Gene ID: 374007 
Additional Characterization:  
Antibody Registry ID: AB_528111 
Additional Information:
Recommended Applications: FACS, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CCD7-1 was deposited to the DSHB by Takeichi, M. / Nakagawa, S. (DSHB Hybridoma Product CCD7-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

13 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication

    Neural crest emigration from the neural tube depends on regulated cadherin expression.
    Takeichi M
    Development (Cambridge, England) 125.15 (1998 Aug): 2963-71.

    IF References

    Neural crest emigration from the neural tube depends on regulated cadherin expression.
    Takeichi M
    Development (Cambridge, England) 125.15 (1998 Aug): 2963-71.

    Regulation of cadherin expression in the chicken neural crest by the Wnt/β-catenin signaling pathway.
    Paulson AF
    Cell adhesion & migration 4.3 (2010 Jul-Sep): 431-8.

    Neural crest emigration from the neural tube depends on regulated cadherin expression.
    Takeichi M
    Development (Cambridge, England) 125.15 (1998 Aug): 2963-71.

    Molecular profiling indicates avian branchiomotor nuclei invade the hindbrain alar plate.
    Redies C
    Neuroscience 128.4 (2004): 785-96.

    Regionalized cadherin-7 expression by radial glia is regulated by Shh and Pax7 during chicken spinal cord development.
    Redies C
    Neuroscience 142.4 (2006 Nov 3): 1133-43.

    Neural crest emigration from the neural tube depends on regulated cadherin expression.
    Takeichi M
    Development (Cambridge, England) 125.15 (1998 Aug): 2963-71.

    Migratory neural crest cell αN-catenin impacts chick trigeminal ganglia formation.
    Taneyhill LA
    Developmental biology 392.2 (2014 Aug 15): 295-307.

    A common developmental plan for neocortical gene-expressing neurons in the pallium of the domestic chicken Gallus gallus domesticus and the Chinese softshell turtle Pelodiscus sinensis.
    Hirata T
    Frontiers in neuroanatomy 8. (2014): 20.

    TrkB/BDNF signalling patterns the sympathetic nervous system.
    Kulesa PM
    Nature communications 6. (2015 Sep 25): 8281.

    Prototypical type I E-cadherin and type II cadherin-7 mediate very distinct adhesiveness through their extracellular domains.
    Dufour S
    The Journal of biological chemistry 281.5 (2006 Feb 3): 2901-10.

    Regulation of cadherin expression in the chicken neural crest by the Wnt/β-catenin signaling pathway.
    Paulson AF
    Cell adhesion & migration 4.3 (2010 Jul-Sep): 431-8.

    Identification and characterization of a soluble cadherin-7 isoform produced by alternative splicing.
    Shirabe K
    The Journal of biological chemistry 277.49 (2002 Dec 6): 47679-85.

    Cadherin expression in the developing chicken cochlea.
    Redies C
    Developmental dynamics : an official publication of the American Association of Anatomists 236.8 (2007 Aug): 2331-7.

    Cadherins guide migrating Purkinje cells to specific parasagittal domains during cerebellar development.
    Redies C
    Molecular and cellular neurosciences 25.1 (2004 Jan): 138-52.

    Pax3 and Pax7 interact reciprocally and regulate the expression of cadherin-7 through inducing neuron differentiation in the developing chicken spinal cord.
    Redies C
    The Journal of comparative neurology 524.5 (2016 Apr 1): 940-62.

    WB References

    Neural crest emigration from the neural tube depends on regulated cadherin expression.
    Takeichi M
    Development (Cambridge, England) 125.15 (1998 Aug): 2963-71.

    Identification and characterization of a soluble cadherin-7 isoform produced by alternative splicing.
    Shirabe K
    The Journal of biological chemistry 277.49 (2002 Dec 6): 47679-85.

    IHC References
    IP References
    FACS References
    Epitope Map References

    Neural crest emigration from the neural tube depends on regulated cadherin expression.
    Takeichi M
    Development (Cambridge, England) 125.15 (1998 Aug): 2963-71.

    All References

    Molecular profiling indicates avian branchiomotor nuclei invade the hindbrain alar plate.
    Redies C
    Neuroscience 128.4 (2004): 785-96.

    Identification and characterization of a soluble cadherin-7 isoform produced by alternative splicing.
    Shirabe K
    The Journal of biological chemistry 277.49 (2002 Dec 6): 47679-85.

    Data on the effects of N-cadherin perturbation on the expression of type II cadherin proteins and major signaling pathways.
    Rogers CD
    Data in brief 20. (2018 Oct): 419-425.

    Neural crest emigration from the neural tube depends on regulated cadherin expression.
    Takeichi M
    Development (Cambridge, England) 125.15 (1998 Aug): 2963-71.

    Regulation of cadherin expression in the chicken neural crest by the Wnt/β-catenin signaling pathway.
    Paulson AF
    Cell adhesion & migration 4.3 (2010 Jul-Sep): 431-8.

    Regionalized cadherin-7 expression by radial glia is regulated by Shh and Pax7 during chicken spinal cord development.
    Redies C
    Neuroscience 142.4 (2006 Nov 3): 1133-43.

    Migratory neural crest cell αN-catenin impacts chick trigeminal ganglia formation.
    Taneyhill LA
    Developmental biology 392.2 (2014 Aug 15): 295-307.

    A common developmental plan for neocortical gene-expressing neurons in the pallium of the domestic chicken Gallus gallus domesticus and the Chinese softshell turtle Pelodiscus sinensis.
    Hirata T
    Frontiers in neuroanatomy 8. (2014): 20.

    TrkB/BDNF signalling patterns the sympathetic nervous system.
    Kulesa PM
    Nature communications 6. (2015 Sep 25): 8281.

    Prototypical type I E-cadherin and type II cadherin-7 mediate very distinct adhesiveness through their extracellular domains.
    Dufour S
    The Journal of biological chemistry 281.5 (2006 Feb 3): 2901-10.

    Cadherin expression in the developing chicken cochlea.
    Redies C
    Developmental dynamics : an official publication of the American Association of Anatomists 236.8 (2007 Aug): 2331-7.

    Cadherins guide migrating Purkinje cells to specific parasagittal domains during cerebellar development.
    Redies C
    Molecular and cellular neurosciences 25.1 (2004 Jan): 138-52.

    Pax3 and Pax7 interact reciprocally and regulate the expression of cadherin-7 through inducing neuron differentiation in the developing chicken spinal cord.
    Redies C
    The Journal of comparative neurology 524.5 (2016 Apr 1): 940-62.

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