D3

(0) No Reviews yet
$40.00
SKU: D3
View product citations for antibody D3 on CiteAb

In Stock

Available: 92

DSHB Data Sheet

Catalog Fields

Antigen: Desmin, intermediate filament
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Fischman, D.A.
Isotype: MIgA, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Cornell University Medical College
Positive Tested Species Reactivity: Chicken, Hamster, Mouse, Rat
Depositors Notes: The D3 antibody is positive in stage 26 chick limb bud, day 19 rat limb bud and day 12 rat myotome. D3 also stained primary cultures of embryonic cardiac myocytes and H.H. stage 17-18 embryonic chick hearts.
Antigen Molecular Weight: 53.2 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: DES
Immunogen: Acetic acid solubilized demins from chicken gizzard
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2
Epitope Location or Sequence: N-terminal rod domain
Uniprot ID: P02542 
Immunogen Sequence: Full length protein
Entrez Gene ID: 395906 
Additional Characterization:
Antibody Registry ID: AB_528195 
Additional Information: RID:AB_528195
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
D3 was deposited to the DSHB by Fischman, D.A. (DSHB Hybridoma Product D3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

15 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Immunocytochemical analysis of intermediate filaments in embryonic heart cells with monoclonal antibodies to desmin.
    Fischman DA
    The Journal of cell biology 98.6 (1984 Jun): 2179-91.

    Differentiation of myogenic cells in micromass cultures of cells from chick facial primordia.
    Tickle C
    Developmental biology 131.1 (1989 Jan): 189-96.

    Temporal differences in desmin expression between myoblasts from embryonic and adult chicken skeletal muscle.
    Nameroff M
    Differentiation; research in biological diversity 45.1 (1990 Oct): 21-8.

    Myogenic cytodifferentiation of the precardiac mesoderm in the rat.
    Solursh M
    Differentiation; research in biological diversity 47.3 (1991 Aug): 163-72.

    Hearts of dystonia musculorum mice display normal morphological and histological features but show signs of cardiac stress.
    Boudreau-Larivière C
    PloS one 5.3 (2010 Mar 1): e9465.

    BDNF is expressed in skeletal muscle satellite cells and inhibits myogenic differentiation.
    Jasmin BJ
    The Journal of neuroscience : the official journal of the Society for Neuroscience 26.21 (2006 May 24): 5739-49.

    Muscle-specific RING finger-2 (MURF-2) is important for microtubule, intermediate filament and sarcomeric M-line maintenance in striated muscle development.
    Gregorio CC
    Journal of cell science 117.Pt 15 (2004 Jul 1): 3175-88.

    WB References
    IHC References
    IP References

    Regulatory role of arginine-specific mono(ADP-ribosyl)transferase in muscle cells.
    McMahon KK
    Advances in experimental medicine and biology 419. (1997): 305-13.

    Proper perinuclear localization of the TRIM-like protein myospryn requires its binding partner desmin.
    Capetanaki Y
    The Journal of biological chemistry 282.48 (2007 Nov 30): 35211-21.

    ELISA References
    Epitope Map References
    All References

    Differentiation of myogenic cells in micromass cultures of cells from chick facial primordia.
    Tickle C
    Developmental biology 131.1 (1989 Jan): 189-96.

    Monoclonal antibodies to desmin: evidence for stage-dependent intermediate filament immunoreactivity during cardiac and skeletal muscle development.
    Danto SI
    Annals of the New York Academy of Sciences 455. (1985): 167-84.

    A mouse model of rhabdomyosarcoma originating from the adipocyte lineage.
    Olson EN
    Cancer cell 22.4 (2012 Oct 16): 536-46.

    Growth factor regulation of neural chemorepellent Sema3A expression in satellite cell cultures.
    Tatsumi R
    American journal of physiology. Cell physiology 301.5 (2011 Nov): C1270-9.

    High concentrations of HGF inhibit skeletal muscle satellite cell proliferation in vitro by inducing expression of myostatin: a possible mechanism for reestablishing satellite cell quiescence in vivo.
    Allen RE
    American journal of physiology. Cell physiology 298.3 (2010 Mar): C465-76.

    Overexpression of C-terminally but not N-terminally truncated Myb induces fibrosarcomas: a novel nonhematopoietic target cell for the myb oncogene.
    Ewert DL
    Molecular and cellular biology 14.4 (1994 Apr): 2278-90.

    Immunocytochemical analysis of intermediate filaments in embryonic heart cells with monoclonal antibodies to desmin.
    Fischman DA
    The Journal of cell biology 98.6 (1984 Jun): 2179-91.

    Temporal differences in desmin expression between myoblasts from embryonic and adult chicken skeletal muscle.
    Nameroff M
    Differentiation; research in biological diversity 45.1 (1990 Oct): 21-8.

    Myogenic cytodifferentiation of the precardiac mesoderm in the rat.
    Solursh M
    Differentiation; research in biological diversity 47.3 (1991 Aug): 163-72.

    Hearts of dystonia musculorum mice display normal morphological and histological features but show signs of cardiac stress.
    Boudreau-Larivière C
    PloS one 5.3 (2010 Mar 1): e9465.

    BDNF is expressed in skeletal muscle satellite cells and inhibits myogenic differentiation.
    Jasmin BJ
    The Journal of neuroscience : the official journal of the Society for Neuroscience 26.21 (2006 May 24): 5739-49.

    Muscle-specific RING finger-2 (MURF-2) is important for microtubule, intermediate filament and sarcomeric M-line maintenance in striated muscle development.
    Gregorio CC
    Journal of cell science 117.Pt 15 (2004 Jul 1): 3175-88.

    The transition from proliferation to differentiation is delayed in satellite cells from mice lacking MyoD.
    Natanson P
    Developmental biology 210.2 (1999 Jun 15): 440-55.

    Regulatory role of arginine-specific mono(ADP-ribosyl)transferase in muscle cells.
    McMahon KK
    Advances in experimental medicine and biology 419. (1997): 305-13.

    Proper perinuclear localization of the TRIM-like protein myospryn requires its binding partner desmin.
    Capetanaki Y
    The Journal of biological chemistry 282.48 (2007 Nov 30): 35211-21.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review