D76

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$40.00
SKU: D76
View product citations for antibody D76 on CiteAb

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Available: 146

DSHB Data Sheet

Catalog Fields

Antigen: Desmin, intermediate filament
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Fischman, D.A.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Cornell University Medical College
Positive Tested Species Reactivity: Chicken, Fish, Lamb, Mouse, Rat
Depositors Notes: The D76 monoclonal antibodies bind to desmin but recognize different epitopes in all types of muscle including vascular smooth muscle but is unreactive with embryonic cardiac myocytes. D76 also labels electric organs in fish and myoid cells in chick and fish thymus.
Antigen Molecular Weight: 53.2 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: DES
Immunogen: Acetic acid-solubilized desmin from gizzard
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence: a.a. 97-255, probably near the N-terminus of Helix 1A
Uniprot ID: P02542 
Immunogen Sequence: Full length sequence
Entrez Gene ID: 395906 
Additional Characterization:
Antibody Registry ID: AB_528196 
Additional Information: Isopentane-cooled frozen sections or Triton X-100 extracted cultured cells are recommended for immunostaining. RRID: AB_528196
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
D76 was deposited to the DSHB by Fischman, D.A. (DSHB Hybridoma Product D76)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

18 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication

    The Journal of cell biology
    Fischman DA
    The Journal of cell biology 98.6 (1984 Jun): 2179-91.

    IF References

    Proteome science
    Blanco G
    Proteome science 12. (2014): 25.

    FASEB journal : official publication of the Federation of American Societies for Experimental Biology
    Unguez GA
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 20.14 (2006 Dec): 2540.

    Anatomy and embryology
    Benson JM
    Anatomy and embryology 197.6 (1998 Jun): 421-37.

    Journal of comparative physiology. A, Sensory, neural, and behavioral physiology
    Franchina CR
    Journal of comparative physiology. A, Sensory, neural, and behavioral physiology 181.2 (1997 Aug): 111-9.

    Cellular & molecular biology research
    Christ B
    Cellular & molecular biology research 41.4 (1995): 241-9.

    Differentiation; research in biological diversity
    Nameroff M
    Differentiation; research in biological diversity 45.1 (1990 Oct): 21-8.

    The Journal of cell biology
    Fischman DA
    The Journal of cell biology 98.6 (1984 Jun): 2179-91.

    Histochemistry
    Zak R
    Histochemistry 93.4 (1990): 423-8.

    Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
    Velleman SG
    Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 219-220. (2018 May): 58-66.

    Cell motility and the cytoskeleton
    Olmsted JB
    Cell motility and the cytoskeleton 54.4 (2003 Apr): 317-36.

    Proteome science
    Blanco G
    Proteome science 12. (2014): 25.

    WB References

    The Journal of cell biology
    Fischman DA
    The Journal of cell biology 98.6 (1984 Jun): 2179-91.

    American journal of physiology. Heart and circulatory physiology
    Simpson JA
    American journal of physiology. Heart and circulatory physiology 310.5 (2016 Mar 1): H572-86.

    Journal of animal science
    Doumit ME
    Journal of animal science 73.12 (1995 Dec): 3596-607.

    The Journal of physiology
    Baar K
    The Journal of physiology 596.14 (2018 Jul): 2883-2900.

    FASEB journal : official publication of the Federation of American Societies for Experimental Biology
    Unguez GA
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 20.14 (2006 Dec): 2540.

    The Journal of comparative neurology
    Zakon HH
    The Journal of comparative neurology 370.3 (1996 Jul 1): 367-76.

    IHC References

    The Journal of cell biology
    Fischman DA
    The Journal of cell biology 98.6 (1984 Jun): 2179-91.

    Cell and tissue research
    Oláh I
    Cell and tissue research 359.2 (2015 Feb): 489-501.

    Journal of anatomy
    Joss J
    Journal of anatomy 214.1 (2009 Jan): 140-52.

    The Journal of comparative neurology
    Zakon HH
    The Journal of comparative neurology 370.3 (1996 Jul 1): 367-76.

    IP References

    Molecular biology of the cell
    Conover GM
    Molecular biology of the cell 27.24 (2016 Dec 1): 3869-3882.

    Epitope Map References

    The Journal of cell biology
    Ip W
    The Journal of cell biology 106.3 (1988 Mar): 735-45.

    All References

    The Journal of cell biology
    Fischman DA
    The Journal of cell biology 98.6 (1984 Jun): 2179-91.

    Cell and tissue research
    Oláh I
    Cell and tissue research 359.2 (2015 Feb): 489-501.

    Journal of anatomy
    Joss J
    Journal of anatomy 214.1 (2009 Jan): 140-52.

    The Journal of comparative neurology
    Zakon HH
    The Journal of comparative neurology 370.3 (1996 Jul 1): 367-76.

    Proteome science
    Blanco G
    Proteome science 12. (2014): 25.

    FASEB journal : official publication of the Federation of American Societies for Experimental Biology
    Unguez GA
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 20.14 (2006 Dec): 2540.

    Anatomy and embryology
    Benson JM
    Anatomy and embryology 197.6 (1998 Jun): 421-37.

    Journal of comparative physiology. A, Sensory, neural, and behavioral physiology
    Franchina CR
    Journal of comparative physiology. A, Sensory, neural, and behavioral physiology 181.2 (1997 Aug): 111-9.

    Cellular & molecular biology research
    Christ B
    Cellular & molecular biology research 41.4 (1995): 241-9.

    Differentiation; research in biological diversity
    Nameroff M
    Differentiation; research in biological diversity 45.1 (1990 Oct): 21-8.

    Histochemistry
    Zak R
    Histochemistry 93.4 (1990): 423-8.

    Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
    Velleman SG
    Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 219-220. (2018 May): 58-66.

    Cell motility and the cytoskeleton
    Olmsted JB
    Cell motility and the cytoskeleton 54.4 (2003 Apr): 317-36.

    American journal of physiology. Heart and circulatory physiology
    Simpson JA
    American journal of physiology. Heart and circulatory physiology 310.5 (2016 Mar 1): H572-86.

    Journal of animal science
    Doumit ME
    Journal of animal science 73.12 (1995 Dec): 3596-607.

    The Journal of physiology
    Baar K
    The Journal of physiology 596.14 (2018 Jul): 2883-2900.

    Molecular biology of the cell
    Conover GM
    Molecular biology of the cell 27.24 (2016 Dec 1): 3869-3882.

    The Journal of cell biology
    Ip W
    The Journal of cell biology 106.3 (1988 Mar): 735-45.

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