D76
Catalog Fields
Clone ID/Product Name: D76
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: DES
Ab Isotype: MIgG1
Gene Name:
Entrez Gene ID: 395906
Clonality: Monoclonal
Immunogen: Acetic acid-solubilized desmin from gizzard
Clone:
Immunogen Sequence: Full length sequence
Myeloma Strain: SP2/0
Epitope Mapped: Yes
Antigen Name: Desmin, intermediate filament
Epitope Location or Sequence: a.a. 97-255, probably near the N-terminus of Helix 1A
Alternate Antigen Name:
Deposit Date: 4/22/1986
Antigen Molecular Weight: 53.2 kDa
Depositor: Fischman, D.A.
Antigen Sequence:
Depositor Institution: Cornell University Medical College
Antigen Species: Chicken
Depositor Notes: The D76 monoclonal antibodies bind to desmin but recognize different epitopes in all types of muscle including vascular smooth muscle but is unreactive with embryonic cardiac myocytes. D76 also labels electric organs in fish and myoid cells in chick and fish thymus.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Chicken, Fish, Lamb, Mouse, Rat
Additional Information: Isopentane-cooled frozen sections or Triton X-100 extracted cultured cells are recommended for immunostaining. RRID: AB_528196
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
D76 was deposited to the DSHB by Fischman, D.A. (DSHB Hybridoma Product D76)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
19 References
Initial Publication
Immunocytochemical analysis of intermediate filaments in embryonic heart cells with monoclonal antibodies to desmin.
Fischman DA
The Journal of cell biology 98.6 (1984 Jun): 2179-91.