D76

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$40.00
SKU: D76
View product citations for antibody D76 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Desmin, intermediate filament
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Fischman, D.A.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Cornell University Medical College
Positive Tested Species Reactivity: Chicken, Fish, Lamb, Mouse, Rat
Depositors Notes: The D76 monoclonal antibodies bind to desmin but recognize different epitopes in all types of muscle including vascular smooth muscle but is unreactive with embryonic cardiac myocytes. D76 also labels electric organs in fish and myoid cells in chick and fish thymus.
Antigen Molecular Weight: 53.2 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: DES
Immunogen: Acetic acid-solubilized desmin from gizzard
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence: a.a. 97-255, probably near the N-terminus of Helix 1A
Uniprot ID: P02542 
Immunogen Sequence: Full length sequence
Entrez Gene ID: 395906 
Additional Characterization:
Antibody Registry ID: AB_528196 
Additional Information: Isopentane-cooled frozen sections or Triton X-100 extracted cultured cells are recommended for immunostaining. RRID: AB_528196
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
D76 was deposited to the DSHB by Fischman, D.A. (DSHB Hybridoma Product D76)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

18 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Proteomic analysis of laser capture microscopy purified myotendinous junction regions from muscle sections.
    Blanco G
    Proteome science 12. (2014): 25.

    Evidence of post-transcriptional regulation in the maintenance of a partial muscle phenotype by electrogenic cells of S. macrurus.
    Unguez GA
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 20.14 (2006 Dec): 2540.

    Transitions in cell organization and in expression of contractile and extracellular matrix proteins during development of chicken aortic smooth muscle: evidence for a complex spatial and temporal differentiation program.
    Benson JM
    Anatomy and embryology 197.6 (1998 Jun): 421-37.

    Ontogeny of the electric organ discharge and the electric organ in the weakly electric pulse fish Brachyhypopomus pinnicaudatus (Hypopomidae, Gymnotiformes).
    Franchina CR
    Journal of comparative physiology. A, Sensory, neural, and behavioral physiology 181.2 (1997 Aug): 111-9.

    Development of chicken aortic smooth muscle: expression of cytoskeletal and basement membrane proteins defines two distinct cell phenotypes emerging from a common lineage.
    Christ B
    Cellular and molecular biology research 41.4 (1995): 241-9.

    Temporal differences in desmin expression between myoblasts from embryonic and adult chicken skeletal muscle.
    Nameroff M
    Differentiation; research in biological diversity 45.1 (1990 Oct): 21-8.

    Immunocytochemical analysis of intermediate filaments in embryonic heart cells with monoclonal antibodies to desmin.
    Fischman DA
    The Journal of cell biology 98.6 (1984 Jun): 2179-91.

    Arrangement of cytoskeletal filaments at the equator of chicken intrafusal muscle fibers.
    Zak R
    Histochemistry 93.4 (1990): 423-8.

    Effect of incubation temperature on neuropeptide Y and neuropeptide Y receptors in turkey and chicken satellite cells.
    Velleman SG
    Comparative biochemistry and physiology. Part A, Molecular and integrative physiology 219-220. (2018 May): 58-66.

    Muscle-specific microtubule-associated protein 4 is expressed early in myogenesis and is not sufficient to induce microtubule reorganization.
    Olmsted JB
    Cell motility and the cytoskeleton 54.4 (2003 Apr): 317-36.

    Proteomic analysis of laser capture microscopy purified myotendinous junction regions from muscle sections.
    Blanco G
    Proteome science 12. (2014): 25.

    WB References

    Immunocytochemical analysis of intermediate filaments in embryonic heart cells with monoclonal antibodies to desmin.
    Fischman DA
    The Journal of cell biology 98.6 (1984 Jun): 2179-91.

    Dissecting the role of the myofilament in diaphragm dysfunction during the development of heart failure in mice.
    Simpson JA
    American journal of physiology. Heart and circulatory physiology 310.5 (2016 Mar 1): H572-86.

    A muscle hypertrophy condition in lamb (callipyge): characterization of effects on muscle growth and meat quality traits.
    Doumit ME
    Journal of animal science 73.12 (1995 Dec): 3596-607.

    Alterations in the muscle force transfer apparatus in aged rats during unloading and reloading: impact of microRNA-31.
    Baar K
    The Journal of physiology 596.14 (2018 Jul): 2883-2900.

    Evidence of post-transcriptional regulation in the maintenance of a partial muscle phenotype by electrogenic cells of S. macrurus.
    Unguez GA
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 20.14 (2006 Dec): 2540.

    Differential expression of proteins in muscle and electric organ, a muscle derivative.
    Zakon HH
    The Journal of comparative neurology 370.3 (1996 Jul 1): 367-76.

    IHC References

    Immunocytochemical analysis of intermediate filaments in embryonic heart cells with monoclonal antibodies to desmin.
    Fischman DA
    The Journal of cell biology 98.6 (1984 Jun): 2179-91.

    A novel aspect of the structure of the avian thymic medulla.
    Oláh I
    Cell and tissue research 359.2 (2015 Feb): 489-501.

    Cytoskeletal proteins in thymic epithelial cells of the Australian lungfish Neoceratodus forsteri.
    Joss J
    Journal of anatomy 214.1 (2009 Jan): 140-52.

    Differential expression of proteins in muscle and electric organ, a muscle derivative.
    Zakon HH
    The Journal of comparative neurology 370.3 (1996 Jul 1): 367-76.

    IP References

    Nebulette is a powerful cytolinker organizing desmin and actin in mouse hearts.
    Conover GM
    Molecular biology of the cell 27.24 (2016 Dec 1): 3869-3882.

    Epitope Map References

    Modulation of desmin intermediate filament assembly by a monoclonal antibody.
    Ip W
    The Journal of cell biology 106.3 (1988 Mar): 735-45.

    All References

    Immunocytochemical analysis of intermediate filaments in embryonic heart cells with monoclonal antibodies to desmin.
    Fischman DA
    The Journal of cell biology 98.6 (1984 Jun): 2179-91.

    A novel aspect of the structure of the avian thymic medulla.
    Oláh I
    Cell and tissue research 359.2 (2015 Feb): 489-501.

    Cytoskeletal proteins in thymic epithelial cells of the Australian lungfish Neoceratodus forsteri.
    Joss J
    Journal of anatomy 214.1 (2009 Jan): 140-52.

    Differential expression of proteins in muscle and electric organ, a muscle derivative.
    Zakon HH
    The Journal of comparative neurology 370.3 (1996 Jul 1): 367-76.

    Proteomic analysis of laser capture microscopy purified myotendinous junction regions from muscle sections.
    Blanco G
    Proteome science 12. (2014): 25.

    Evidence of post-transcriptional regulation in the maintenance of a partial muscle phenotype by electrogenic cells of S. macrurus.
    Unguez GA
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 20.14 (2006 Dec): 2540.

    Transitions in cell organization and in expression of contractile and extracellular matrix proteins during development of chicken aortic smooth muscle: evidence for a complex spatial and temporal differentiation program.
    Benson JM
    Anatomy and embryology 197.6 (1998 Jun): 421-37.

    Ontogeny of the electric organ discharge and the electric organ in the weakly electric pulse fish Brachyhypopomus pinnicaudatus (Hypopomidae, Gymnotiformes).
    Franchina CR
    Journal of comparative physiology. A, Sensory, neural, and behavioral physiology 181.2 (1997 Aug): 111-9.

    Development of chicken aortic smooth muscle: expression of cytoskeletal and basement membrane proteins defines two distinct cell phenotypes emerging from a common lineage.
    Christ B
    Cellular and molecular biology research 41.4 (1995): 241-9.

    Temporal differences in desmin expression between myoblasts from embryonic and adult chicken skeletal muscle.
    Nameroff M
    Differentiation; research in biological diversity 45.1 (1990 Oct): 21-8.

    Arrangement of cytoskeletal filaments at the equator of chicken intrafusal muscle fibers.
    Zak R
    Histochemistry 93.4 (1990): 423-8.

    Effect of incubation temperature on neuropeptide Y and neuropeptide Y receptors in turkey and chicken satellite cells.
    Velleman SG
    Comparative biochemistry and physiology. Part A, Molecular and integrative physiology 219-220. (2018 May): 58-66.

    Muscle-specific microtubule-associated protein 4 is expressed early in myogenesis and is not sufficient to induce microtubule reorganization.
    Olmsted JB
    Cell motility and the cytoskeleton 54.4 (2003 Apr): 317-36.

    Dissecting the role of the myofilament in diaphragm dysfunction during the development of heart failure in mice.
    Simpson JA
    American journal of physiology. Heart and circulatory physiology 310.5 (2016 Mar 1): H572-86.

    A muscle hypertrophy condition in lamb (callipyge): characterization of effects on muscle growth and meat quality traits.
    Doumit ME
    Journal of animal science 73.12 (1995 Dec): 3596-607.

    Alterations in the muscle force transfer apparatus in aged rats during unloading and reloading: impact of microRNA-31.
    Baar K
    The Journal of physiology 596.14 (2018 Jul): 2883-2900.

    Nebulette is a powerful cytolinker organizing desmin and actin in mouse hearts.
    Conover GM
    Molecular biology of the cell 27.24 (2016 Dec 1): 3869-3882.

    Modulation of desmin intermediate filament assembly by a monoclonal antibody.
    Ip W
    The Journal of cell biology 106.3 (1988 Mar): 735-45.

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