DCSP-1 (ab49)

(0) No Reviews yet
$40.00
SKU: DCSP-1 (ab49)
View product citations for antibody DCSP-1 (ab49) on CiteAb

In Stock

Available: 83

DSHB Data Sheet

Catalog Fields

Antigen: cysteine string protein (CSP), Drosophila common
Hybridoma Cells Available: Yes
Antigen Species: Drosophila melanogaster
Depositor: Buchner, E. / Hofbauer, A.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Wuerzburg
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Stains all synaptic neuropils and synaptic butons.
Antigen Molecular Weight: Predicted: multiple isoforms at 24 and 27 kDa. Observed with mAb DCSP-1: 32, 33, 34, and 36 kDa.
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Csp
Immunogen: Brain homogenate
Alternate Gene Names: CG6395
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X.63-Ag8.653
Epitope Location or Sequence: FTGA, aa 196-203 of isoform 1 (Uniprot ID: Q03751-1).
Uniprot ID: Q03751 
Immunogen Sequence: Full length protein
Entrez Gene ID: 40459 
Additional Characterization:  
Antibody Registry ID: AB_2307345 
Additional Information: This antibody recognizes all known CSP isoforms. CSP is associated with the cytoplasmic surface of synaptic vesicles and is often used as a synaptic vesicle marker. It labels all synapses. See [PMID: 9799436 and 8310297] for a comparison between DCSP-1, DCSP-2 and DCSP3 mAbs. CSP is also known as DnaJ homolog subfamily C member 5 homolog.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DCSP-1 (ab49) was deposited to the DSHB by Buchner, E. / Hofbauer, A. (DSHB Hybridoma Product DCSP-1 (ab49))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

19 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication

    A cysteine-string protein is expressed in retina and brain of Drosophila.
    Buchner E
    Journal of neurogenetics 7.1 (1990 Nov): 15-29.

    IF References
    WB References

    A cysteine-string protein is expressed in retina and brain of Drosophila.
    Buchner E
    Journal of neurogenetics 7.1 (1990 Nov): 15-29.

    Wide distribution of the cysteine string proteins in Drosophila tissues revealed by targeted mutagenesis.
    Buchner E
    Cell and tissue research 294.2 (1998 Nov): 203-17.

    Structure-function analysis of the cysteine string protein in Drosophila: cysteine string, linker and C terminus.
    Buchner E
    The Journal of experimental biology 207.Pt 8 (2004 Mar): 1323-34.

    Glial expression of Swiss cheese (SWS), the Drosophila orthologue of neuropathy target esterase (NTE), is required for neuronal ensheathment and function.
    Kretzschmar D
    Disease models & mechanisms 9.3 (2016 Mar): 283-94.

    Synapsin is required to "boost" memory strength for highly salient events.
    Gerber B
    Learning & memory (Cold Spring Harbor, N.Y.) 23.1 (2016 Jan): 9-20.

    AP180 couples protein retrieval to clathrin-mediated endocytosis of synaptic vesicles.
    Zhang B
    Traffic (Copenhagen, Denmark) 15.4 (2014 Apr): 433-50.

    Differential centrifugation-based biochemical fractionation of the Drosophila adult CNS.
    Holt MG
    Nature protocols 9.12 (2014 Dec): 2796-808.

    Deregulation of the Egfr/Ras signaling pathway induces age-related brain degeneration in the Drosophila mutant vap.
    Schneuwly S
    Molecular biology of the cell 14.1 (2003 Jan): 241-50.

    Drosophila photoreceptors express cysteine peptidase tan.
    Hovemann BT
    The Journal of comparative neurology 500.4 (2007 Feb 1): 601-11.

    Drosophila Hsc70-4 is critical for neurotransmitter exocytosis in vivo.
    Zinsmaier KE
    Neuron 30.2 (2001 May): 475-88.

    IHC References

    A cysteine-string protein is expressed in retina and brain of Drosophila.
    Buchner E
    Journal of neurogenetics 7.1 (1990 Nov): 15-29.

    Wide distribution of the cysteine string proteins in Drosophila tissues revealed by targeted mutagenesis.
    Buchner E
    Cell and tissue research 294.2 (1998 Nov): 203-17.

    Structure-function analysis of the cysteine string protein in Drosophila: cysteine string, linker and C terminus.
    Buchner E
    The Journal of experimental biology 207.Pt 8 (2004 Mar): 1323-34.

    The Wuerzburg hybridoma library against Drosophila brain.
    Buchner E
    Journal of neurogenetics 23.1-2 (2009): 78-91.

    Deregulation of the Egfr/Ras signaling pathway induces age-related brain degeneration in the Drosophila mutant vap.
    Schneuwly S
    Molecular biology of the cell 14.1 (2003 Jan): 241-50.

    Age-dependent memory loss, synaptic pathology and altered brain plasticity in the Drosophila mutant cardinal accumulating 3-hydroxykynurenine.
    Riederer P
    Journal of neural transmission (Vienna, Austria : 1996) 107.5 (2000): 581-601.

    Distinct functions of neuronal synaptobrevin in developing and mature fly photoreceptors.
    Heisenberg M
    Journal of neurobiology 66.12 (2006 Oct): 1271-84.

    Paralysis and early death in cysteine string protein mutants of Drosophila.
    Benzer S
    Science (New York, N.Y.) 263.5149 (1994 Feb 18): 977-80.

    IP References
    Epitope Map References
    All References

    A cysteine-string protein is expressed in retina and brain of Drosophila.
    Buchner E
    Journal of neurogenetics 7.1 (1990 Nov): 15-29.

    Wide distribution of the cysteine string proteins in Drosophila tissues revealed by targeted mutagenesis.
    Buchner E
    Cell and tissue research 294.2 (1998 Nov): 203-17.

    Structure-function analysis of the cysteine string protein in Drosophila: cysteine string, linker and C terminus.
    Buchner E
    The Journal of experimental biology 207.Pt 8 (2004 Mar): 1323-34.

    The Wuerzburg hybridoma library against Drosophila brain.
    Buchner E
    Journal of neurogenetics 23.1-2 (2009): 78-91.

    Deregulation of the Egfr/Ras signaling pathway induces age-related brain degeneration in the Drosophila mutant vap.
    Schneuwly S
    Molecular biology of the cell 14.1 (2003 Jan): 241-50.

    Age-dependent memory loss, synaptic pathology and altered brain plasticity in the Drosophila mutant cardinal accumulating 3-hydroxykynurenine.
    Riederer P
    Journal of neural transmission (Vienna, Austria : 1996) 107.5 (2000): 581-601.

    Distinct functions of neuronal synaptobrevin in developing and mature fly photoreceptors.
    Heisenberg M
    Journal of neurobiology 66.12 (2006 Oct): 1271-84.

    Paralysis and early death in cysteine string protein mutants of Drosophila.
    Benzer S
    Science (New York, N.Y.) 263.5149 (1994 Feb 18): 977-80.

    Loss of the Coffin-Lowry syndrome-associated gene RSK2 alters ERK activity, synaptic function and axonal transport in Drosophila motoneurons.
    Raabe T
    Disease models & mechanisms 8.11 (2015 Nov): 1389-400.

    The GTPase Rab26 links synaptic vesicles to the autophagy pathway.
    Jahn R
    eLife 4. (2015 Feb 2): e05597.

    AP180 couples protein retrieval to clathrin-mediated endocytosis of synaptic vesicles.
    Zhang B
    Traffic (Copenhagen, Denmark) 15.4 (2014 Apr): 433-50.

    Drosophila Fip200 is an essential regulator of autophagy that attenuates both growth and aging.
    Lee JH
    Autophagy 9.8 (2013 Aug): 1201-13.

    Increased expression of the Drosophila vesicular glutamate transporter leads to excess glutamate release and a compensatory decrease in quantal content.
    DiAntonio A
    The Journal of neuroscience : the official journal of the Society for Neuroscience 24.46 (2004 Nov 17): 10466-74.

    The atypical cadherin flamingo regulates synaptogenesis and helps prevent axonal and synaptic degeneration in Drosophila.
    Zhang B
    Molecular and cellular neurosciences 34.4 (2007 Apr): 662-78.

    Drosophila Hsc70-4 is critical for neurotransmitter exocytosis in vivo.
    Zinsmaier KE
    Neuron 30.2 (2001 May): 475-88.

    Glial expression of Swiss cheese (SWS), the Drosophila orthologue of neuropathy target esterase (NTE), is required for neuronal ensheathment and function.
    Kretzschmar D
    Disease models & mechanisms 9.3 (2016 Mar): 283-94.

    Synapsin is required to "boost" memory strength for highly salient events.
    Gerber B
    Learning & memory (Cold Spring Harbor, N.Y.) 23.1 (2016 Jan): 9-20.

    Differential centrifugation-based biochemical fractionation of the Drosophila adult CNS.
    Holt MG
    Nature protocols 9.12 (2014 Dec): 2796-808.

    Drosophila photoreceptors express cysteine peptidase tan.
    Hovemann BT
    The Journal of comparative neurology 500.4 (2007 Feb 1): 601-11.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review