DCSP-1 (ab49)
Catalog Fields
Antigen: cysteine string protein (CSP), Drosophila common
Hybridoma Cells Available: Yes
Antigen Species: Drosophila melanogaster
Depositor: Buchner, E. / Hofbauer, A.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Wuerzburg
Positive Tested Species Reactivity: Drosophila
Depositors Notes: Stains all synaptic neuropils and synaptic butons.
Antigen Molecular Weight: Predicted: multiple isoforms at 24 and 27 kDa. Observed with mAb DCSP-1: 32, 33, 34, and 36 kDa.
Predicted Species Reactivity:
Gene: Csp
Immunogen: Brain homogenate
Alternate Gene Names: CG6395
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X.63-Ag8.653
Epitope Location or Sequence: FTGA, aa 196-203 of isoform 1 (Uniprot ID: Q03751-1).
Uniprot ID: Q03751
Immunogen Sequence: Full length protein
Entrez Gene ID: 40459
Antibody Registry ID: AB_2307345
Additional Information: This antibody recognizes all known CSP isoforms. CSP is associated with the cytoplasmic surface of synaptic vesicles and is often used as a synaptic vesicle marker. It labels all synapses. See [PMID: 9799436 and 8310297] for a comparison between DCSP-1, DCSP-2 and DCSP3 mAbs. CSP is also known as DnaJ homolog subfamily C member 5 homolog.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DCSP-1 (ab49) was deposited to the DSHB by Buchner, E. / Hofbauer, A. (DSHB Hybridoma Product DCSP-1 (ab49))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
19 References
Initial Publication
A cysteine-string protein is expressed in retina and brain of Drosophila.
Buchner E
Journal of neurogenetics 7.1 (1990 Nov): 15-29.