DCSP-2 (6D6)

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SKU: DCSP-2 (6D6)
View product citations for antibody DCSP-2 (6D6) on CiteAb

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Available: 60

DSHB Data Sheet

Catalog Fields

Antigen: cysteine string protein (CSP), Drosophila common
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Benzer, S.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: California Institute of Technology
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: Predicted: multiple isoforms at 24 and 27 kDa. Observed with mAb DCSP-2: 32, 33, 34, and 36 kDa.
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Csp
Immunogen: Recombinant CSP/GST fusion protein
Alternate Gene Names: CG6395
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain:
Epitope Location or Sequence: REQAGGQP, aa 218-222 of isoform 1 (Uniprot ID: Q03751-1).
Uniprot ID: Q03751 
Immunogen Sequence: Full length protein
Entrez Gene ID: 40459 
Additional Characterization:
Antibody Registry ID: AB_528183 
Additional Information: This antibody recognizes all known CSP isoforms and labels all synapses. See [PMID: 9799436 and 8310297] for a comparison between DCSP-1, DCSP-2 and DCSP3 mAbs. DCSP-2 is often used as a synaptic vesicle marker. CSP is also known as DnaJ homolog subfamily C member 5 homolog.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DCSP-2 (6D6) was deposited to the DSHB by Benzer, S. (DSHB Hybridoma Product DCSP-2 (6D6))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

21 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    Epitope Map References
    All References

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