DCSP-2 (6D6)

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$40.00
SKU: DCSP-2 (6D6)
View product citations for antibody DCSP-2 (6D6) on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: cysteine string protein (CSP), Drosophila common
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Benzer, S.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: California Institute of Technology
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: Predicted: multiple isoforms at 24 and 27 kDa. Observed with mAb DCSP-2: 32, 33, 34, and 36 kDa.
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Csp
Immunogen: Recombinant CSP/GST fusion protein
Alternate Gene Names: CG6395
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain:
Epitope Location or Sequence: REQAGGQP, aa 218-222 of isoform 1 (Uniprot ID: Q03751-1).
Uniprot ID: Q03751 
Immunogen Sequence: Full length protein
Entrez Gene ID: 40459 
Additional Characterization:  
Antibody Registry ID: AB_528183 
Additional Information: This antibody recognizes all known CSP isoforms and labels all synapses. See [PMID: 9799436 and 8310297] for a comparison between DCSP-1, DCSP-2 and DCSP3 mAbs. DCSP-2 is often used as a synaptic vesicle marker. CSP is also known as DnaJ homolog subfamily C member 5 homolog.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DCSP-2 (6D6) was deposited to the DSHB by Benzer, S. (DSHB Hybridoma Product DCSP-2 (6D6))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

21 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication

    Paralysis and early death in cysteine string protein mutants of Drosophila.
    Benzer S
    Science (New York, N.Y.) 263.5149 (1994 Feb 18): 977-80.

    IF References

    Cholesterol and F-actin are required for clustering of recycling synaptic vesicle proteins in the presynaptic plasma membrane.
    Charlton MP
    The Journal of physiology 592.4 (2014 Feb 15): 621-33.

    The metalloprotease tolloid-related and its TGF-beta-like substrate Dawdle regulate Drosophila motoneuron axon guidance.
    O'Connor MB
    Development (Cambridge, England) 133.24 (2006 Dec): 4969-79.

    Drosophila huntingtin-interacting protein 14 is a presynaptic protein required for photoreceptor synaptic transmission and expression of the palmitoylated proteins synaptosome-associated protein 25 and cysteine string protein.
    Isacoff EY
    The Journal of neuroscience : the official journal of the Society for Neuroscience 27.47 (2007 Nov 21): 12874-83.

    dAcsl, the Drosophila ortholog of acyl-CoA synthetase long-chain family member 3 and 4, inhibits synapse growth by attenuating bone morphogenetic protein signaling via endocytic recycling.
    Zhang YQ
    The Journal of neuroscience : the official journal of the Society for Neuroscience 34.8 (2014 Feb 19): 2785-96.

    Modulation of dADAR-dependent RNA editing by the Drosophila fragile X mental retardation protein.
    Jongens TA
    Nature neuroscience 14.12 (2011 Oct 30): 1517-24.

    Spectrin mutations that cause spinocerebellar ataxia type 5 impair axonal transport and induce neurodegeneration in Drosophila.
    Hays TS
    The Journal of cell biology 189.1 (2010 Apr 5): 143-58.

    Reticulon-like-1, the Drosophila orthologue of the hereditary spastic paraplegia gene reticulon 2, is required for organization of endoplasmic reticulum and of distal motor axons.
    O'Kane CJ
    Human molecular genetics 21.15 (2012 Aug 1): 3356-65.

    The ALS-associated proteins FUS and TDP-43 function together to affect Drosophila locomotion and life span.
    McCabe BD
    The Journal of clinical investigation 121.10 (2011 Oct): 4118-26.

    Drosophila FMRP regulates microtubule network formation and axonal transport of mitochondria.
    Zhang YQ
    Human molecular genetics 20.1 (2011 Jan 1): 51-63.

    Role of kinesin-1-based microtubule sliding in Drosophila nervous system development.
    Gelfand VI
    Proceedings of the National Academy of Sciences of the United States of America 113.34 (2016 Aug 23): E4985-94.

    Angelman Syndrome Protein Ube3a Regulates Synaptic Growth and Endocytosis by Inhibiting BMP Signaling in Drosophila.
    Zhang YQ
    PLoS genetics 12.5 (2016 May): e1006062.

    Acsl, the Drosophila ortholog of intellectual-disability-related ACSL4, inhibits synaptic growth by altered lipids.
    Zhang YQ
    Journal of cell science 129.21 (2016 Nov 1): 4034-4045.

    Regulation of Fasciclin II and synaptic terminal development by the splicing factor beag.
    McCabe BD
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.20 (2012 May 16): 7058-73.

    The Microtubule Regulatory Protein Stathmin Is Required to Maintain the Integrity of Axonal Microtubules in Drosophila.
    Goldstein LS
    PloS one 8.6 (2013): e68324.

    Miro's N-terminal GTPase domain is required for transport of mitochondria into axons and dendrites.
    Zinsmaier KE
    The Journal of neuroscience : the official journal of the Society for Neuroscience 35.14 (2015 Apr 8): 5754-71.

    Dysfunction of <i>GRAP</i>, encoding the GRB2-related adaptor protein, is linked to sensorineural hearing loss.
    Tekin M
    Proceedings of the National Academy of Sciences of the United States of America 116.4 (2019 Jan 22): 1347-1352.

    WB References
    IHC References
    Epitope Map References
    All References

    Paralysis and early death in cysteine string protein mutants of Drosophila.
    Benzer S
    Science (New York, N.Y.) 263.5149 (1994 Feb 18): 977-80.

    Wide distribution of the cysteine string proteins in Drosophila tissues revealed by targeted mutagenesis.
    Buchner E
    Cell and tissue research 294.2 (1998 Nov): 203-17.

    Drosophila adducin regulates Dlg phosphorylation and targeting of Dlg to the synapse and epithelial membrane.
    Krieger C
    Developmental biology 357.2 (2011 Sep 15): 392-403.

    Cholesterol and F-actin are required for clustering of recycling synaptic vesicle proteins in the presynaptic plasma membrane.
    Charlton MP
    The Journal of physiology 592.4 (2014 Feb 15): 621-33.

    The metalloprotease tolloid-related and its TGF-beta-like substrate Dawdle regulate Drosophila motoneuron axon guidance.
    O'Connor MB
    Development (Cambridge, England) 133.24 (2006 Dec): 4969-79.

    Drosophila huntingtin-interacting protein 14 is a presynaptic protein required for photoreceptor synaptic transmission and expression of the palmitoylated proteins synaptosome-associated protein 25 and cysteine string protein.
    Isacoff EY
    The Journal of neuroscience : the official journal of the Society for Neuroscience 27.47 (2007 Nov 21): 12874-83.

    dAcsl, the Drosophila ortholog of acyl-CoA synthetase long-chain family member 3 and 4, inhibits synapse growth by attenuating bone morphogenetic protein signaling via endocytic recycling.
    Zhang YQ
    The Journal of neuroscience : the official journal of the Society for Neuroscience 34.8 (2014 Feb 19): 2785-96.

    Modulation of dADAR-dependent RNA editing by the Drosophila fragile X mental retardation protein.
    Jongens TA
    Nature neuroscience 14.12 (2011 Oct 30): 1517-24.

    Spectrin mutations that cause spinocerebellar ataxia type 5 impair axonal transport and induce neurodegeneration in Drosophila.
    Hays TS
    The Journal of cell biology 189.1 (2010 Apr 5): 143-58.

    Reticulon-like-1, the Drosophila orthologue of the hereditary spastic paraplegia gene reticulon 2, is required for organization of endoplasmic reticulum and of distal motor axons.
    O'Kane CJ
    Human molecular genetics 21.15 (2012 Aug 1): 3356-65.

    The ALS-associated proteins FUS and TDP-43 function together to affect Drosophila locomotion and life span.
    McCabe BD
    The Journal of clinical investigation 121.10 (2011 Oct): 4118-26.

    Drosophila FMRP regulates microtubule network formation and axonal transport of mitochondria.
    Zhang YQ
    Human molecular genetics 20.1 (2011 Jan 1): 51-63.

    Role of kinesin-1-based microtubule sliding in Drosophila nervous system development.
    Gelfand VI
    Proceedings of the National Academy of Sciences of the United States of America 113.34 (2016 Aug 23): E4985-94.

    Angelman Syndrome Protein Ube3a Regulates Synaptic Growth and Endocytosis by Inhibiting BMP Signaling in Drosophila.
    Zhang YQ
    PLoS genetics 12.5 (2016 May): e1006062.

    Acsl, the Drosophila ortholog of intellectual-disability-related ACSL4, inhibits synaptic growth by altered lipids.
    Zhang YQ
    Journal of cell science 129.21 (2016 Nov 1): 4034-4045.

    Regulation of Fasciclin II and synaptic terminal development by the splicing factor beag.
    McCabe BD
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.20 (2012 May 16): 7058-73.

    The Microtubule Regulatory Protein Stathmin Is Required to Maintain the Integrity of Axonal Microtubules in Drosophila.
    Goldstein LS
    PloS one 8.6 (2013): e68324.

    Miro's N-terminal GTPase domain is required for transport of mitochondria into axons and dendrites.
    Zinsmaier KE
    The Journal of neuroscience : the official journal of the Society for Neuroscience 35.14 (2015 Apr 8): 5754-71.

    Dysfunction of <i>GRAP</i>, encoding the GRB2-related adaptor protein, is linked to sensorineural hearing loss.
    Tekin M
    Proceedings of the National Academy of Sciences of the United States of America 116.4 (2019 Jan 22): 1347-1352.

    Structure-function analysis of the cysteine string protein in Drosophila: cysteine string, linker and C terminus.
    Buchner E
    The Journal of experimental biology 207.Pt 8 (2004 Mar): 1323-34.

    Neurexin-1 is required for synapse formation and larvae associative learning in Drosophila.
    Xie W
    FEBS letters 581.13 (2007 May 29): 2509-16.

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