Antigen: cysteine string protein (CSP), Drosophila common
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Benzer, S.
Host Species: mouse
Depositors Institution: California Institute of Technology
Positive Tested Species Reactivity: Drosophila
Antigen Molecular Weight: Predicted: multiple isoforms at 24 and 27 kDa. Observed with mAb DCSP-2: 32, 33, 34, and 36 kDa.
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: Recombinant CSP/GST fusion protein
Alternate Gene Names: CG6395
Alternate Antibody Name:
Alternate Antigen Name:
Epitope Mapped: Yes
Epitope Location or Sequence: REQAGGQP, aa 218-222 of isoform 1 (Uniprot ID: Q03751-1).
Uniprot ID: Q03751
Immunogen Sequence: Full length protein
Entrez Gene ID: 40459
Antibody Registry ID: AB_528183
Additional Information: This antibody recognizes all known CSP isoforms and labels all synapses. See [PMID: 9799436 and 8310297] for a comparison between DCSP-1, DCSP-2 and DCSP3 mAbs. DCSP-2 is often used as a synaptic vesicle marker. CSP is also known as DnaJ homolog subfamily C member 5 homolog.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DCSP-2 (6D6) was deposited to the DSHB by Benzer, S. (DSHB Hybridoma Product DCSP-2 (6D6))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
Epitope Map References