DCSP-3 (1G12)

(0) No Reviews yet
$40.00
SKU: DCSP-3 (1G12)
View product citations for antibody DCSP-3 (1G12) on CiteAb

In Stock

Available: 38

DSHB Data Sheet

Catalog Fields

Antigen: Cysteine string protein (C-terminus)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Benzer, S.
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: California Institute of Technology, Division of Biology
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: Multiple isoforms at 24 and 27 kDa. Observed with mAb DCSP-2: 32, 33, 34, and 36 kDa.
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Csp
Immunogen: Recombinant CSP/GST fusion protein
Alternate Gene Names: CG6395
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain:
Epitope Location or Sequence: YTPDMVNQKY (a.a. 239-249)
Uniprot ID: Q03751 
Immunogen Sequence: Full length protein
Entrez Gene ID: 40459 
Additional Characterization:  
Antibody Registry ID: AB_528184 
Additional Information: This antibody will not recognize some isoforms that show a variant C-terminal like splicing isoform 3 (Uniprot ID: Q03751-3), and preferentially labels synapses of photoreceptors. See [PMID: 9799436 and 8310297] for a comparison between DCSP-1, DCSP-2 and DCSP3 mAbs. CSP is also known as DnaJ homolog subfamily C member 5 homolog. RRID: AB_528184. FBgn0004179
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DCSP-3 (1G12) was deposited to the DSHB by Benzer, S. (DSHB Hybridoma Product DCSP-3 (1G12))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

10 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication

    Paralysis and early death in cysteine string protein mutants of Drosophila.
    Benzer S
    Science (New York, N.Y.) 263.5149 (1994 Feb 18): 977-80.

    IF References
    WB References

    Paralysis and early death in cysteine string protein mutants of Drosophila.
    Benzer S
    Science (New York, N.Y.) 263.5149 (1994 Feb 18): 977-80.

    Wide distribution of the cysteine string proteins in Drosophila tissues revealed by targeted mutagenesis.
    Buchner E
    Cell and tissue research 294.2 (1998 Nov): 203-17.

    Mutation of the Drosophila vesicular GABA transporter disrupts visual figure detection.
    Krantz DE
    The Journal of experimental biology 213.Pt 10 (2010 May): 1717-30.

    IHC References

    Paralysis and early death in cysteine string protein mutants of Drosophila.
    Benzer S
    Science (New York, N.Y.) 263.5149 (1994 Feb 18): 977-80.

    Wide distribution of the cysteine string proteins in Drosophila tissues revealed by targeted mutagenesis.
    Buchner E
    Cell and tissue research 294.2 (1998 Nov): 203-17.

    Epitope Map References
    All References

    Paralysis and early death in cysteine string protein mutants of Drosophila.
    Benzer S
    Science (New York, N.Y.) 263.5149 (1994 Feb 18): 977-80.

    Wide distribution of the cysteine string proteins in Drosophila tissues revealed by targeted mutagenesis.
    Buchner E
    Cell and tissue research 294.2 (1998 Nov): 203-17.

    Tbc1d15-17 regulates synaptic development at the Drosophila neuromuscular junction.
    Lee S
    Molecules and cells 36.2 (2013 Aug): 163-8.

    Diet and energy-sensing inputs affect TorC1-mediated axon misrouting but not TorC2-directed synapse growth in a Drosophila model of tuberous sclerosis.
    Selleck SB
    PloS one 7.2 (2012): e30722.

    dTip60 HAT activity controls synaptic bouton expansion at the Drosophila neuromuscular junction.
    Elefant F
    PloS one 6.10 (2011): e26202.

    Negative regulation of active zone assembly by a newly identified SR protein kinase.
    Davis GW
    PLoS biology 7.9 (2009 Sep): e1000193.

    Drosophila Atlastin regulates the stability of muscle microtubules and is required for synapse development.
    Lee S
    Developmental biology 330.2 (2009 Jun 15): 250-62.

    Mutation of the Drosophila vesicular GABA transporter disrupts visual figure detection.
    Krantz DE
    The Journal of experimental biology 213.Pt 10 (2010 May): 1717-30.

    Terminal axonal arborization and synaptic bouton formation critically rely on abp1 and the arp2/3 complex.
    Kessels MM
    PloS one 9.5 (2014): e97692.

    Structure-function analysis of the cysteine string protein in Drosophila: cysteine string, linker and C terminus.
    Buchner E
    The Journal of experimental biology 207.Pt 8 (2004 Mar): 1323-34.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review