DCSP-3 (1G12)

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$40.00
SKU: DCSP-3 (1G12)
View product citations for antibody DCSP-3 (1G12) on CiteAb

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Available: 12

DSHB Data Sheet

Catalog Fields

Antigen: cysteine string protein (CSP), Drosophila C-terminal
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Benzer, S.
Isotype: MIgG2a
Depositors Institution: California Institute of Technology
Host Species: mouse
Gene: Csp
Positive Tested Species Reactivity: Drosophila
Alternate Gene Names: CG6395
Antigen Molecular Weight: Predicted: multiple isoforms at 24 and 27 kDa. Observed with mAb DCSP-3: 33 and 36 kDa.
Clonality: Monoclonal
Immunogen: Recombinant CSP/GST fusion protein
Epitope Mapped: Yes
Uniprot ID: Q03751 
Epitope Location or Sequence: YTPDMVNQKY, aa 239-249 of isoform 1 (Uniprot ID: Q03751-1).
Entrez Gene ID: 40459 
Immunogen Sequence: Full length protein
Antibody Registry ID: AB_528184 
Additional Information: This antibody will not recognize some isoforms that show a variant C-terminal like splicing isoform 3 (Uniprot ID: Q03751-3), and preferentially labels synapses of photoreceptors. See [PMID: 9799436 and 8310297] for a comparison between DCSP-1, DCSP-2 and DCSP3 mAbs. CSP is also known as DnaJ homolog subfamily C member 5 homolog.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DCSP-3 (1G12) was deposited to the DSHB by Benzer, S. (DSHB Hybridoma Product DCSP-3 (1G12))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

10 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication

    Paralysis and early death in cysteine string protein mutants of Drosophila.
    Benzer S
    Science (New York, N.Y.) 263.5149 (1994 Feb 18): 977-80.

    IF References
    WB References

    Paralysis and early death in cysteine string protein mutants of Drosophila.
    Benzer S
    Science (New York, N.Y.) 263.5149 (1994 Feb 18): 977-80.

    Wide distribution of the cysteine string proteins in Drosophila tissues revealed by targeted mutagenesis.
    Buchner E
    Cell and tissue research 294.2 (1998 Nov): 203-17.

    Mutation of the Drosophila vesicular GABA transporter disrupts visual figure detection.
    Krantz DE
    The Journal of experimental biology 213.Pt 10 (2010 May): 1717-30.

    IHC References

    Paralysis and early death in cysteine string protein mutants of Drosophila.
    Benzer S
    Science (New York, N.Y.) 263.5149 (1994 Feb 18): 977-80.

    Wide distribution of the cysteine string proteins in Drosophila tissues revealed by targeted mutagenesis.
    Buchner E
    Cell and tissue research 294.2 (1998 Nov): 203-17.

    Epitope Map References
    All References

    Paralysis and early death in cysteine string protein mutants of Drosophila.
    Benzer S
    Science (New York, N.Y.) 263.5149 (1994 Feb 18): 977-80.

    Wide distribution of the cysteine string proteins in Drosophila tissues revealed by targeted mutagenesis.
    Buchner E
    Cell and tissue research 294.2 (1998 Nov): 203-17.

    Tbc1d15-17 regulates synaptic development at the Drosophila neuromuscular junction.
    Lee S
    Molecules and cells 36.2 (2013 Aug): 163-8.

    Diet and energy-sensing inputs affect TorC1-mediated axon misrouting but not TorC2-directed synapse growth in a Drosophila model of tuberous sclerosis.
    Selleck SB
    PloS one 7.2 (2012): e30722.

    dTip60 HAT activity controls synaptic bouton expansion at the Drosophila neuromuscular junction.
    Elefant F
    PloS one 6.10 (2011): e26202.

    Negative regulation of active zone assembly by a newly identified SR protein kinase.
    Davis GW
    PLoS biology 7.9 (2009 Sep): e1000193.

    Drosophila Atlastin regulates the stability of muscle microtubules and is required for synapse development.
    Lee S
    Developmental biology 330.2 (2009 Jun 15): 250-62.

    Mutation of the Drosophila vesicular GABA transporter disrupts visual figure detection.
    Krantz DE
    The Journal of experimental biology 213.Pt 10 (2010 May): 1717-30.

    Terminal axonal arborization and synaptic bouton formation critically rely on abp1 and the arp2/3 complex.
    Kessels MM
    PloS one 9.5 (2014): e97692.

    Structure-function analysis of the cysteine string protein in Drosophila: cysteine string, linker and C terminus.
    Buchner E
    The Journal of experimental biology 207.Pt 8 (2004 Mar): 1323-34.

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