F64A6B4
Catalog Fields
Product Name/ID: F64A6B4
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name:
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_532380
Clonality: Monoclonal
Immunogen: NK homeodomain protein, Nkx6.1
Clone:
Immunogen Sequence:
Myeloma Strain: Fox-NY
Epitope Mapped: No
Antigen Name: Nkx6.1
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 3/15/2006
Antigen Molecular Weight: 44-46 kDa
Depositor: Madsen, O.D.
Antigen Sequence:
Depositor Institution: Hagedorn Research Institute
Antigen Species: rat
Depositor Notes: Immunohistology note: section of adult pancreas; staining of β-cell nuclei. Antibody competition: F64A6B4 competes for binding with F65A2, but can bind simultaneously with F55A10 or F55A12. Specificity note: mouse, rat, and human; other species not tested (see F55A10 for additional species). Does not cross-react to Nkx6.2. Antigen note: NK homeobox protein Nkx6.1 is a transcription factor. Immunoblotting note: Western blot gives a double band of 44 and 46 kDa. Functional effects note: transcription factor required for neural and pancreatic endocrine development. IHC note: early embryonic pancreatic epithelium; ventral neural tube; stomach mesenchyme and adult β cells.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Human, Mouse, Rat
Additional Information:
Recommended Applications: FACS, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
F64A6B4 was deposited to the DSHB by Madsen, O.D. (DSHB Hybridoma Product F64A6B4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
2 References
Initial Publication
Generation and characterization of monoclonal antibodies against the transcription factor Nkx6.1.
Jorgensen MC
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 54.5 (2006 May): 567-74.