40-1a

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$40.00
SKU: 40-1a
View product citations for antibody 40-1a on CiteAb

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Available: 194

DSHB Data Sheet

Catalog Fields

Antigen: beta-galactosidase
Hybridoma Cells Available: Yes
Antigen Species: E. coli
Depositor: Sanes, J.R.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Harvard University
Positive Tested Species Reactivity: E. coli
Depositors Notes: Recognizes the E.coli beta-galatosidase sequence in beta-gal fusion proteins.
Antigen Molecular Weight: Predicted: 116 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: LacZ
Immunogen: Beta-galactosidase (E.coli)
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain:
Epitope Location or Sequence:
Uniprot ID: P00722 
Immunogen Sequence:
Entrez Gene ID: 945006 
Additional Characterization:
Antibody Registry ID: AB_2314509 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
40-1a was deposited to the DSHB by Sanes, J.R. (DSHB Hybridoma Product 40-1a)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

25 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication
    IF References

    The encephalomyocarditis virus internal ribosome entry site allows efficient coexpression of two genes from a recombinant provirus in cultured cells and in embryos.
    Majors JE
    Molecular and cellular biology 11.12 (1991 Dec): 5848-59.

    Proximal to distal cell communication in the Drosophila leg provides a basis for an intercalary mechanism of limb patterning.
    Hayashi S
    Development (Cambridge, England) 126.15 (1999 Aug): 3407-13.

    Several levels of EGF receptor signaling during photoreceptor specification in wild-type, Ellipse, and null mutant Drosophila.
    Baker NE
    Developmental biology 205.1 (1999 Jan 1): 129-44.

    Role of the EGFR/Ras/Raf pathway in specification of photoreceptor cells in the Drosophila retina.
    Baker NE
    Development (Cambridge, England) 128.7 (2001 Apr): 1183-91.

    Proneural enhancement by Notch overcomes Suppressor-of-Hairless repressor function in the developing Drosophila eye.
    Baker NE
    Current biology : CB 11.5 (2001 Mar 6): 330-8.

    Exocrine specific expression of Connexin32 is dependent on the basic helix-loop-helix transcription factor Mist1.
    Konieczny SF
    Journal of cell science 116.Pt 16 (2003 Aug 15): 3315-25.

    Scabrous and Gp150 are endosomal proteins that regulate Notch activity.
    Baker NE
    Development (Cambridge, England) 130.13 (2003 Jul): 2819-27.

    Notch activity in neural cells triggered by a mutant allele with altered glycosylation.
    Baker NE
    Development (Cambridge, England) 130.13 (2003 Jul): 2829-40.

    Production of gurken in the nurse cells is sufficient for axis determination in the Drosophila oocyte.
    Nilson LA
    Development (Cambridge, England) 132.10 (2005 May): 2345-53.

    Math5 defines the ganglion cell competence state in a subpopulation of retinal progenitor cells exiting the cell cycle.
    Glaser T
    Developmental biology 365.2 (2012 May 15): 395-413.

    Imp associates with squid and Hrp48 and contributes to localized expression of gurken in the oocyte.
    Macdonald PM
    Molecular and cellular biology 26.24 (2006 Dec): 9508-16.

    Short-term activation of the Jun N-terminal kinase pathway in apoptosis-deficient cells of Drosophila induces tumorigenesis.
    Morata G
    Nature communications 9.1 (2018 Apr 18): 1541.

    14-3-3 proteins regulate Tctp-Rheb interaction for organ growth in Drosophila.
    Choi KW
    Nature communications 7. (2016 May 6): 11501.

    Role of the ABC transporter Mdr49 in Hedgehog signaling and germ cell migration.
    Schedl P
    Development (Cambridge, England) 143.12 (2016 Jun 15): 2111-20.

    Identification and characterization of early photoreceptor cis-regulatory elements and their relation to Onecut1.
    Emerson MM
    Neural development 13.1 (2018 Nov 22): 26.

    Reproduction disrupts stem cell homeostasis in testes of aged male Drosophila via an induced microenvironment.
    Pi H
    PLoS genetics 15.7 (2019 Jul): e1008062.

    WB References
    IHC References
    All References

    The scabrous protein can act as an extracellular antagonist of notch signaling in the Drosophila wing.
    Baker NE
    Current biology : CB 10.15 (2000 Jul 27-Aug 10): 931-4.

    Odd paired transcriptional activation of decapentaplegic in the Drosophila eye/antennal disc is cell autonomous but indirect.
    Hursh DA
    Developmental biology 343.1-2 (2010 Jul 1): 167-77.

    Nucleo-cytoplasmic shuttling of Drosophila Hairless/Su(H) heterodimer as a means of regulating Notch dependent transcription.
    Maier D
    Biochimica et biophysica acta. Molecular cell research 1866.10 (2019 Oct): 1520-1532.

    Characterization of TNF-induced cell death in Drosophila reveals caspase- and JNK-dependent necrosis and its role in tumor suppression.
    Fan Y
    Cell death and disease 10.8 (2019 Aug 14): 613.

    The encephalomyocarditis virus internal ribosome entry site allows efficient coexpression of two genes from a recombinant provirus in cultured cells and in embryos.
    Majors JE
    Molecular and cellular biology 11.12 (1991 Dec): 5848-59.

    Proximal to distal cell communication in the Drosophila leg provides a basis for an intercalary mechanism of limb patterning.
    Hayashi S
    Development (Cambridge, England) 126.15 (1999 Aug): 3407-13.

    Several levels of EGF receptor signaling during photoreceptor specification in wild-type, Ellipse, and null mutant Drosophila.
    Baker NE
    Developmental biology 205.1 (1999 Jan 1): 129-44.

    Role of the EGFR/Ras/Raf pathway in specification of photoreceptor cells in the Drosophila retina.
    Baker NE
    Development (Cambridge, England) 128.7 (2001 Apr): 1183-91.

    Proneural enhancement by Notch overcomes Suppressor-of-Hairless repressor function in the developing Drosophila eye.
    Baker NE
    Current biology : CB 11.5 (2001 Mar 6): 330-8.

    Exocrine specific expression of Connexin32 is dependent on the basic helix-loop-helix transcription factor Mist1.
    Konieczny SF
    Journal of cell science 116.Pt 16 (2003 Aug 15): 3315-25.

    Scabrous and Gp150 are endosomal proteins that regulate Notch activity.
    Baker NE
    Development (Cambridge, England) 130.13 (2003 Jul): 2819-27.

    Notch activity in neural cells triggered by a mutant allele with altered glycosylation.
    Baker NE
    Development (Cambridge, England) 130.13 (2003 Jul): 2829-40.

    Production of gurken in the nurse cells is sufficient for axis determination in the Drosophila oocyte.
    Nilson LA
    Development (Cambridge, England) 132.10 (2005 May): 2345-53.

    Math5 defines the ganglion cell competence state in a subpopulation of retinal progenitor cells exiting the cell cycle.
    Glaser T
    Developmental biology 365.2 (2012 May 15): 395-413.

    Imp associates with squid and Hrp48 and contributes to localized expression of gurken in the oocyte.
    Macdonald PM
    Molecular and cellular biology 26.24 (2006 Dec): 9508-16.

    Short-term activation of the Jun N-terminal kinase pathway in apoptosis-deficient cells of Drosophila induces tumorigenesis.
    Morata G
    Nature communications 9.1 (2018 Apr 18): 1541.

    14-3-3 proteins regulate Tctp-Rheb interaction for organ growth in Drosophila.
    Choi KW
    Nature communications 7. (2016 May 6): 11501.

    Role of the ABC transporter Mdr49 in Hedgehog signaling and germ cell migration.
    Schedl P
    Development (Cambridge, England) 143.12 (2016 Jun 15): 2111-20.

    Identification and characterization of early photoreceptor cis-regulatory elements and their relation to Onecut1.
    Emerson MM
    Neural development 13.1 (2018 Nov 22): 26.

    Reproduction disrupts stem cell homeostasis in testes of aged male Drosophila via an induced microenvironment.
    Pi H
    PLoS genetics 15.7 (2019 Jul): e1008062.

    TPC1 has two variant isoforms, and their removal has different effects on endo-lysosomal functions compared to loss of TPC2.
    Parrington J
    Molecular and cellular biology 34.21 (2014 Nov): 3981-92.

    The Toxoplasma homolog of Plasmodium apical membrane antigen-1 (AMA-1) is a microneme protein secreted in response to elevated intracellular calcium levels.
    Ward GE
    Molecular and biochemical parasitology 111.1 (2000 Nov): 15-30.

    An exonic splicing silencer downstream of the 3' splice site A2 is required for efficient human immunodeficiency virus type 1 replication.
    Stoltzfus CM
    Journal of virology 79.16 (2005 Aug): 10478-86.

    The EGF receptor defines domains of cell cycle progression and survival to regulate cell number in the developing Drosophila eye.
    Yu SY
    Cell 104.5 (2001 Mar 9): 699-708.

    Cell cycle withdrawal, progression, and cell survival regulation by EGFR and its effectors in the differentiating Drosophila eye.
    Baker NE
    Developmental cell 4.3 (2003 Mar): 359-69.

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