40-1a

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$45.00 to $425.00
SKU: 40-1a
View product citations for antibody 40-1a on CiteAb

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Available: 64

DSHB Data Sheet

Catalog Fields

Product Name/ID: 40-1a
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: LacZ
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_528100 
Uniprot ID: P00722 
RRID:  
Entrez Gene ID: 945006 
Clonality: Monoclonal
Immunogen: Beta-galactosidase (E.coli)
Clone:
Immunogen Sequence:
Myeloma Strain:
Epitope Mapped: No
Antigen Name: beta-galactosidase
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 7/22/1992
Antigen Molecular Weight: Predicted: 116 kDa
Depositor: Sanes, J.R.
Antigen Sequence:
Depositor Institution: Harvard University
Antigen Species: E. coli
Depositor Notes: Recognizes the E.coli beta-galatosidase sequence in beta-gal fusion proteins.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: E. coli
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
40-1a was deposited to the DSHB by Sanes, J.R. (DSHB Hybridoma Product 40-1a)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

30 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication
    IF References

    The encephalomyocarditis virus internal ribosome entry site allows efficient coexpression of two genes from a recombinant provirus in cultured cells and in embryos.
    Majors JE
    Molecular and cellular biology 11.12 (1991 Dec): 5848-59.

    Proximal to distal cell communication in the Drosophila leg provides a basis for an intercalary mechanism of limb patterning.
    Hayashi S
    Development (Cambridge, England) 126.15 (1999 Aug): 3407-13.

    Several levels of EGF receptor signaling during photoreceptor specification in wild-type, Ellipse, and null mutant Drosophila.
    Baker NE
    Developmental biology 205.1 (1999 Jan 1): 129-44.

    Role of the EGFR/Ras/Raf pathway in specification of photoreceptor cells in the Drosophila retina.
    Baker NE
    Development (Cambridge, England) 128.7 (2001 Apr): 1183-91.

    Proneural enhancement by Notch overcomes Suppressor-of-Hairless repressor function in the developing Drosophila eye.
    Baker NE
    Current biology : CB 11.5 (2001 Mar 6): 330-8.

    Exocrine specific expression of Connexin32 is dependent on the basic helix-loop-helix transcription factor Mist1.
    Konieczny SF
    Journal of cell science 116.Pt 16 (2003 Aug 15): 3315-25.

    Scabrous and Gp150 are endosomal proteins that regulate Notch activity.
    Baker NE
    Development (Cambridge, England) 130.13 (2003 Jul): 2819-27.

    Notch activity in neural cells triggered by a mutant allele with altered glycosylation.
    Baker NE
    Development (Cambridge, England) 130.13 (2003 Jul): 2829-40.

    Production of gurken in the nurse cells is sufficient for axis determination in the Drosophila oocyte.
    Nilson LA
    Development (Cambridge, England) 132.10 (2005 May): 2345-53.

    Math5 defines the ganglion cell competence state in a subpopulation of retinal progenitor cells exiting the cell cycle.
    Glaser T
    Developmental biology 365.2 (2012 May 15): 395-413.

    Imp associates with squid and Hrp48 and contributes to localized expression of gurken in the oocyte.
    Macdonald PM
    Molecular and cellular biology 26.24 (2006 Dec): 9508-16.

    Short-term activation of the Jun N-terminal kinase pathway in apoptosis-deficient cells of Drosophila induces tumorigenesis.
    Morata G
    Nature communications 9.1 (2018 Apr 18): 1541.

    14-3-3 proteins regulate Tctp-Rheb interaction for organ growth in Drosophila.
    Choi KW
    Nature communications 7. (2016 May 6): 11501.

    Role of the ABC transporter Mdr49 in Hedgehog signaling and germ cell migration.
    Schedl P
    Development (Cambridge, England) 143.12 (2016 Jun 15): 2111-20.

    Identification and characterization of early photoreceptor cis-regulatory elements and their relation to Onecut1.
    Emerson MM
    Neural development 13.1 (2018 Nov 22): 26.

    Reproduction disrupts stem cell homeostasis in testes of aged male Drosophila via an induced microenvironment.
    Pi H
    PLoS genetics 15.7 (2019 Jul): e1008062.

    Glia-derived temporal signals orchestrate neurogenesis in the Drosophila mushroom body.
    Ho MS
    Proceedings of the National Academy of Sciences of the United States of America 118.23 (2021 Jun 8): .

    Neuroglian regulates Drosophila intestinal stem cell proliferation through enhanced signaling via the epidermal growth factor receptor.
    Jones DL
    Stem cell reports 16.6 (2021 Jun 8): 1584-1597.

    Germline soma communication mediated by gap junction proteins regulates epithelial morphogenesis.
    Prasad M
    PLoS genetics 17.8 (2021 Aug): e1009685.

    WB References
    IHC References
    All References

    The scabrous protein can act as an extracellular antagonist of notch signaling in the Drosophila wing.
    Baker NE
    Current biology : CB 10.15 (2000 Jul 27-Aug 10): 931-4.

    Odd paired transcriptional activation of decapentaplegic in the Drosophila eye/antennal disc is cell autonomous but indirect.
    Hursh DA
    Developmental biology 343.1-2 (2010 Jul 1): 167-77.

    Nucleo-cytoplasmic shuttling of Drosophila Hairless/Su(H) heterodimer as a means of regulating Notch dependent transcription.
    Maier D
    Biochimica et biophysica acta. Molecular cell research 1866.10 (2019 Oct): 1520-1532.

    Characterization of TNF-induced cell death in Drosophila reveals caspase- and JNK-dependent necrosis and its role in tumor suppression.
    Fan Y
    Cell death & disease 10.8 (2019 Aug 14): 613.

    A PI4KIIIα protein complex is required for cell viability during Drosophila wing development.
    Raghu P
    Developmental biology 462.2 (2020 Jun 15): 208-222.

    Systemic and local effect of the Drosophila headcase gene and its role in stress protection of Adult Progenitor Cells.
    Casanova J
    PLoS genetics 17.2 (2021 Feb): e1009362.

    The encephalomyocarditis virus internal ribosome entry site allows efficient coexpression of two genes from a recombinant provirus in cultured cells and in embryos.
    Majors JE
    Molecular and cellular biology 11.12 (1991 Dec): 5848-59.

    Proximal to distal cell communication in the Drosophila leg provides a basis for an intercalary mechanism of limb patterning.
    Hayashi S
    Development (Cambridge, England) 126.15 (1999 Aug): 3407-13.

    Several levels of EGF receptor signaling during photoreceptor specification in wild-type, Ellipse, and null mutant Drosophila.
    Baker NE
    Developmental biology 205.1 (1999 Jan 1): 129-44.

    Role of the EGFR/Ras/Raf pathway in specification of photoreceptor cells in the Drosophila retina.
    Baker NE
    Development (Cambridge, England) 128.7 (2001 Apr): 1183-91.

    Proneural enhancement by Notch overcomes Suppressor-of-Hairless repressor function in the developing Drosophila eye.
    Baker NE
    Current biology : CB 11.5 (2001 Mar 6): 330-8.

    Exocrine specific expression of Connexin32 is dependent on the basic helix-loop-helix transcription factor Mist1.
    Konieczny SF
    Journal of cell science 116.Pt 16 (2003 Aug 15): 3315-25.

    Scabrous and Gp150 are endosomal proteins that regulate Notch activity.
    Baker NE
    Development (Cambridge, England) 130.13 (2003 Jul): 2819-27.

    Notch activity in neural cells triggered by a mutant allele with altered glycosylation.
    Baker NE
    Development (Cambridge, England) 130.13 (2003 Jul): 2829-40.

    Production of gurken in the nurse cells is sufficient for axis determination in the Drosophila oocyte.
    Nilson LA
    Development (Cambridge, England) 132.10 (2005 May): 2345-53.

    Math5 defines the ganglion cell competence state in a subpopulation of retinal progenitor cells exiting the cell cycle.
    Glaser T
    Developmental biology 365.2 (2012 May 15): 395-413.

    Imp associates with squid and Hrp48 and contributes to localized expression of gurken in the oocyte.
    Macdonald PM
    Molecular and cellular biology 26.24 (2006 Dec): 9508-16.

    Short-term activation of the Jun N-terminal kinase pathway in apoptosis-deficient cells of Drosophila induces tumorigenesis.
    Morata G
    Nature communications 9.1 (2018 Apr 18): 1541.

    14-3-3 proteins regulate Tctp-Rheb interaction for organ growth in Drosophila.
    Choi KW
    Nature communications 7. (2016 May 6): 11501.

    Role of the ABC transporter Mdr49 in Hedgehog signaling and germ cell migration.
    Schedl P
    Development (Cambridge, England) 143.12 (2016 Jun 15): 2111-20.

    Identification and characterization of early photoreceptor cis-regulatory elements and their relation to Onecut1.
    Emerson MM
    Neural development 13.1 (2018 Nov 22): 26.

    Reproduction disrupts stem cell homeostasis in testes of aged male Drosophila via an induced microenvironment.
    Pi H
    PLoS genetics 15.7 (2019 Jul): e1008062.

    Glia-derived temporal signals orchestrate neurogenesis in the Drosophila mushroom body.
    Ho MS
    Proceedings of the National Academy of Sciences of the United States of America 118.23 (2021 Jun 8): .

    Neuroglian regulates Drosophila intestinal stem cell proliferation through enhanced signaling via the epidermal growth factor receptor.
    Jones DL
    Stem cell reports 16.6 (2021 Jun 8): 1584-1597.

    Germline soma communication mediated by gap junction proteins regulates epithelial morphogenesis.
    Prasad M
    PLoS genetics 17.8 (2021 Aug): e1009685.

    TPC1 has two variant isoforms, and their removal has different effects on endo-lysosomal functions compared to loss of TPC2.
    Parrington J
    Molecular and cellular biology 34.21 (2014 Nov): 3981-92.

    The Toxoplasma homolog of Plasmodium apical membrane antigen-1 (AMA-1) is a microneme protein secreted in response to elevated intracellular calcium levels.
    Ward GE
    Molecular and biochemical parasitology 111.1 (2000 Nov): 15-30.

    An exonic splicing silencer downstream of the 3' splice site A2 is required for efficient human immunodeficiency virus type 1 replication.
    Stoltzfus CM
    Journal of virology 79.16 (2005 Aug): 10478-86.

    The EGF receptor defines domains of cell cycle progression and survival to regulate cell number in the developing Drosophila eye.
    Yu SY
    Cell 104.5 (2001 Mar 9): 699-708.

    Cell cycle withdrawal, progression, and cell survival regulation by EGFR and its effectors in the differentiating Drosophila eye.
    Baker NE
    Developmental cell 4.3 (2003 Mar): 359-69.

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