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View product citations for antibody GS-9A8 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: insulin (proinsulin)
Hybridoma Cells Available: No
Antigen Species: Human
Depositor: Madsen, O.D.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Hagedorn Research Institute
Positive Tested Species Reactivity: Canine, Human, Monkey, Mouse, Rat
Depositors Notes: This antibody binds to the B-C junction of proinsulin of human and monkey and stains only intact, non-processed proinsulin found in the immature secretory granules of islet beta-cells. It does not cross-react to insulin nor C-peptide. Cross reaction to rodent proinsulins requires aldehyde fixation.
Antigen Molecular Weight: Predicted: 9.4 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: INS
Immunogen: Recombinant human proinsulin coupled to bovine thyroglobulin
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0-Ag14
Epitope Location or Sequence: B-C junction of proinsulin (aa 26-37)
Uniprot ID: P01308 
Immunogen Sequence:
Entrez Gene ID: 3630 
Additional Characterization:  
Antibody Registry ID: AB_532383 
Additional Information: Doesn't recognize bovine or porcine proinsulin.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
GS-9A8 was deposited to the DSHB by Madsen, O.D. (DSHB Hybridoma Product GS-9A8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

14 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FFPE References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References

    Analysis artefacts of the INS-IGF2 fusion transcript.
    Madsen OD
    BMC molecular biology 16. (2015 Jul 29): 13.

    IHC References
    IP References
    FFPE References

    Sel1L-Hrd1 ER-associated degradation maintains β cell identity via TGF-β signaling.
    Qi L
    The Journal of clinical investigation 130.7 (2020 Jul 1): 3499-3510.

    Epitope Map References
    All References

    Proteolytic maturation of insulin is a post-Golgi event which occurs in acidifying clathrin-coated secretory vesicles.
    Perrelet A
    Cell 49.6 (1987 Jun 19): 865-8.

    Proinsulin-specific monoclonal antibodies. Immunocytochemical application as beta-cell markers and as probes for conversion.
    Madsen OD
    Diabetes 36.10 (1987 Oct): 1203-11.

    Hypoglycemic Syndrome without Hyperinsulinemia. A Diagnostic Challenge.
    Escalada J
    Endocrine pathology 27.1 (2016 Mar): 50-4.

    Nesidioblastosis coexisting with non-functioning islet cell tumour in an adult.
    Sperti C
    Endokrynologia Polska 66.4 (2015): 356-60.

    Sel1L-Hrd1 ER-associated degradation maintains β cell identity via TGF-β signaling.
    Qi L
    The Journal of clinical investigation 130.7 (2020 Jul 1): 3499-3510.

    Direct identification of prohormone conversion site in insulin-secreting cells.
    Perrelet A
    Cell 42.2 (1985 Sep): 671-81.

    Human islets contain four distinct subtypes of β cells.
    Grompe M
    Nature communications 7. (2016 Jul 11): 11756.

    Analysis artefacts of the INS-IGF2 fusion transcript.
    Madsen OD
    BMC molecular biology 16. (2015 Jul 29): 13.

    Characterization of Antibodies to Products of Proinsulin Processing Using Immunofluorescence Staining of Pancreas in Multiple Species.
    Kieffer TJ
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 63.8 (2015 Aug): 646-62.

    β-Cell Insulin Secretion Requires the Ubiquitin Ligase COP1.
    Dixit VM
    Cell 163.6 (2015 Dec 3): 1457-67.

    Enhanced insulin secretion and improved glucose tolerance in mice with homozygous inactivation of the Na(+)K(+)2Cl(-) co-transporter 1.
    Di Fulvio M
    The Journal of endocrinology 215.1 (2012 Oct): 59-70.

    Persistence of Pancreatic Insulin mRNA Expression and Proinsulin Protein in Type 1 Diabetes Pancreata.
    Atkinson M
    Cell metabolism 26.3 (2017 Sep 5): 568-575.e3.

    The production and characterization of monoclonal antibodies specific for human proinsulin using a sensitive microdot assay procedure.
    Steiner DF
    Endocrinology 113.6 (1983 Dec): 2135-44.

    Human proinsulin-specific antigenic determinants identified by monoclonal antibodies.
    Steiner DF
    Diabetes 33.10 (1984 Oct): 1012-6.

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