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View product citations for antibody GS-9A8 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: insulin (proinsulin)
Depositor: Madsen, O.D.
Antigen Species: Human
Depositors Institution: Hagedorn Research Institute
Isotype: MIgG1
Depositors Notes: This antibody binds to the B-C junction of proinsulin of human and monkey and stains only intact, non-processed proinsulin found in the immature secretory granules of islet beta-cells. It does not cross-react to insulin nor C-peptide. Cross reaction to rodent proinsulins requires aldehyde fixation.
Host Species: mouse
Gene: INS
Positive Tested Species Reactivity: Canine, Human, Monkey, Mouse, Rat
Clonality: Monoclonal
Antigen Molecular Weight: Predicted: 9.4 kDa
Epitope Mapped: Yes
Immunogen: Recombinant human proinsulin coupled to bovine thyroglobulin
Epitope Location or Sequence: B-C junction of proinsulin (aa 26-37)
Myeloma Strain: SP2/0-Ag14
Additional Information: Doesn't recognize bovine or porcine proinsulin.
Uniprot ID: P01308 
Entrez Gene ID: 3630 
Antibody Registry ID: AB_532383 
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
GS-9A8 was deposited to the DSHB by Madsen, O.D. (DSHB Hybridoma Product GS-9A8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

13 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References

    Analysis artefacts of the INS-IGF2 fusion transcript.
    Madsen OD
    BMC molecular biology 16. (2015 Jul 29): 13.

    IHC References
    IP References
    Epitope Map References
    All References

    Proteolytic maturation of insulin is a post-Golgi event which occurs in acidifying clathrin-coated secretory vesicles.
    Perrelet A
    Cell 49.6 (1987 Jun 19): 865-8.

    Proinsulin-specific monoclonal antibodies. Immunocytochemical application as beta-cell markers and as probes for conversion.
    Madsen OD
    Diabetes 36.10 (1987 Oct): 1203-11.

    Hypoglycemic Syndrome without Hyperinsulinemia. A Diagnostic Challenge.
    Escalada J
    Endocrine pathology 27.1 (2016 Mar): 50-4.

    Nesidioblastosis coexisting with non-functioning islet cell tumour in an adult.
    Sperti C
    Endokrynologia Polska 66.4 (2015): 356-60.

    Direct identification of prohormone conversion site in insulin-secreting cells.
    Perrelet A
    Cell 42.2 (1985 Sep): 671-81.

    Human islets contain four distinct subtypes of β cells.
    Grompe M
    Nature communications 7. (2016 Jul 11): 11756.

    Analysis artefacts of the INS-IGF2 fusion transcript.
    Madsen OD
    BMC molecular biology 16. (2015 Jul 29): 13.

    Characterization of Antibodies to Products of Proinsulin Processing Using Immunofluorescence Staining of Pancreas in Multiple Species.
    Kieffer TJ
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 63.8 (2015 Aug): 646-62.

    β-Cell Insulin Secretion Requires the Ubiquitin Ligase COP1.
    Dixit VM
    Cell 163.6 (2015 Dec 3): 1457-67.

    Enhanced insulin secretion and improved glucose tolerance in mice with homozygous inactivation of the Na(+)K(+)2Cl(-) co-transporter 1.
    Di Fulvio M
    The Journal of endocrinology 215.1 (2012 Oct): 59-70.

    Persistence of Pancreatic Insulin mRNA Expression and Proinsulin Protein in Type 1 Diabetes Pancreata.
    Atkinson M
    Cell metabolism 26.3 (2017 Sep 5): 568-575.e3.

    The production and characterization of monoclonal antibodies specific for human proinsulin using a sensitive microdot assay procedure.
    Steiner DF
    Endocrinology 113.6 (1983 Dec): 2135-44.

    Human proinsulin-specific antigenic determinants identified by monoclonal antibodies.
    Steiner DF
    Diabetes 33.10 (1984 Oct): 1012-6.

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