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SKU: GS-9A8-s

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Available: 141

DSHB Data Sheet

Catalog Fields

Product Name/ID: GS-9A8
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: INS
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_532383 
Uniprot ID: P01308 
Entrez Gene ID: 3630 
Clonality: Monoclonal
Immunogen: Recombinant human proinsulin coupled to bovine thyroglobulin
Immunogen Sequence:
Myeloma Strain: SP2/0-Ag14
Epitope Mapped: Yes
Antigen Name: insulin (proinsulin)
Epitope Location or Sequence: B-C junction of proinsulin (aa 26-37)
Alternate Antigen Name:
Deposit Date: 1/23/2006
Antigen Molecular Weight: Predicted: 9.4 kDa
Depositor: Madsen, O.D.
Antigen Sequence:
Depositor Institution: Hagedorn Research Institute
Antigen Species: Human
Depositor Notes: This antibody binds to the B-C junction of proinsulin of human and monkey and stains only intact, non-processed proinsulin found in the immature secretory granules of islet beta-cells. It does not cross-react to insulin nor C-peptide. Cross reaction to rodent proinsulins requires aldehyde fixation.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Canine, Human, Monkey, Mouse, Rat
Additional Information: Doesn't recognize bovine or porcine proinsulin.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
GS-9A8 was deposited to the DSHB by Madsen, O.D. (DSHB Hybridoma Product GS-9A8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

14 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FFPE References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References

    Analysis artefacts of the INS-IGF2 fusion transcript.
    Madsen OD
    BMC molecular biology 16. (2015 Jul 29): 13.

    IHC References
    IP References
    FFPE References

    Sel1L-Hrd1 ER-associated degradation maintains β cell identity via TGF-β signaling.
    Qi L
    The Journal of clinical investigation 130.7 (2020 Jul 1): 3499-3510.

    Epitope Map References
    All References

    Proteolytic maturation of insulin is a post-Golgi event which occurs in acidifying clathrin-coated secretory vesicles.
    Perrelet A
    Cell 49.6 (1987 Jun 19): 865-8.

    Proinsulin-specific monoclonal antibodies. Immunocytochemical application as beta-cell markers and as probes for conversion.
    Madsen OD
    Diabetes 36.10 (1987 Oct): 1203-11.

    Hypoglycemic Syndrome without Hyperinsulinemia. A Diagnostic Challenge.
    Escalada J
    Endocrine pathology 27.1 (2016 Mar): 50-4.

    Nesidioblastosis coexisting with non-functioning islet cell tumour in an adult.
    Sperti C
    Endokrynologia Polska 66.4 (2015): 356-60.

    Sel1L-Hrd1 ER-associated degradation maintains β cell identity via TGF-β signaling.
    Qi L
    The Journal of clinical investigation 130.7 (2020 Jul 1): 3499-3510.

    Direct identification of prohormone conversion site in insulin-secreting cells.
    Perrelet A
    Cell 42.2 (1985 Sep): 671-81.

    Human islets contain four distinct subtypes of β cells.
    Grompe M
    Nature communications 7. (2016 Jul 11): 11756.

    Analysis artefacts of the INS-IGF2 fusion transcript.
    Madsen OD
    BMC molecular biology 16. (2015 Jul 29): 13.

    Characterization of Antibodies to Products of Proinsulin Processing Using Immunofluorescence Staining of Pancreas in Multiple Species.
    Kieffer TJ
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 63.8 (2015 Aug): 646-62.

    β-Cell Insulin Secretion Requires the Ubiquitin Ligase COP1.
    Dixit VM
    Cell 163.6 (2015 Dec 3): 1457-67.

    Enhanced insulin secretion and improved glucose tolerance in mice with homozygous inactivation of the Na(+)K(+)2Cl(-) co-transporter 1.
    Di Fulvio M
    The Journal of endocrinology 215.1 (2012 Oct): 59-70.

    Persistence of Pancreatic Insulin mRNA Expression and Proinsulin Protein in Type 1 Diabetes Pancreata.
    Atkinson M
    Cell metabolism 26.3 (2017 Sep 5): 568-575.e3.

    The production and characterization of monoclonal antibodies specific for human proinsulin using a sensitive microdot assay procedure.
    Steiner DF
    Endocrinology 113.6 (1983 Dec): 2135-44.

    Human proinsulin-specific antigenic determinants identified by monoclonal antibodies.
    Steiner DF
    Diabetes 33.10 (1984 Oct): 1012-6.

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