F6A11

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SKU: F6A11-s

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DSHB Data Sheet

Catalog Fields

Product Name/ID: F6A11
Available to For-Profits: Yes
Alternate Antibody Name: F6
Gene Name: Pdx1
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_1157904 
Uniprot ID: P52947 
RRID:  
Entrez Gene ID: 29535 
Clonality: Monoclonal
Immunogen: Recombinant GST/Pdx1 (aa 215-283) fusion protein
Clone:
Immunogen Sequence: Partial protein
Myeloma Strain: Fox-NY
Epitope Mapped: Yes
Antigen Name: pancreatic duodenal homeobox protein 1 (Pdx1)
Epitope Location or Sequence: C-terminal aa 215-283.
Alternate Antigen Name:
Deposit Date: 2/28/2007
Antigen Molecular Weight: Predicted: 31kDa; Apparent: 39 kDa and additional band at 70 kDa
Depositor: Madsen, O.D.
Antigen Sequence:
Depositor Institution: Hagedorn Research Institute
Antigen Species: Rat
Depositor Notes: Pdx1 is a homeobox transcription factor required for pancreas formation. F6A11 doesn't recognize human Pdx1.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Mouse, Rat
Additional Information: F6A11 has been deemed superior to F109-D12 for IHC by the depositor.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, FACS, FFPE, Immunofluorescence, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
F6A11 was deposited to the DSHB by Madsen, O.D. (DSHB Hybridoma Product F6A11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

4 References

  • Initial Publication
  • IF References
  • WB References
  • ELISA References
  • FFPE References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication

    Novel monoclonal antibodies against Pdx1 reveal feedback regulation of Pdx1 protein levels.
    Hald J
    European journal of histochemistry : EJH 54.2 (2010 Apr 15): e19.

    IF References

    Novel monoclonal antibodies against Pdx1 reveal feedback regulation of Pdx1 protein levels.
    Hald J
    European journal of histochemistry : EJH 54.2 (2010 Apr 15): e19.

    Sexually dimorphic diet-induced insulin resistance in obese tissue inhibitor of metalloproteinase-2 (TIMP-2)-deficient mice.
    Pratley RE
    Endocrinology 152.4 (2011 Apr): 1300-13.

    Transcriptional dynamics of endodermal organ formation.
    Melton DA
    Developmental dynamics : an official publication of the American Association of Anatomists 238.1 (2009 Jan): 29-42.

    PDX1(LOW) MAFA(LOW) β-cells contribute to islet function and insulin release.
    Hodson DJ
    Nature communications 12.1 (2021 Jan 29): 674.

    WB References
    ELISA References

    Novel monoclonal antibodies against Pdx1 reveal feedback regulation of Pdx1 protein levels.
    Hald J
    European journal of histochemistry : EJH 54.2 (2010 Apr 15): e19.

    FFPE References

    Novel monoclonal antibodies against Pdx1 reveal feedback regulation of Pdx1 protein levels.
    Hald J
    European journal of histochemistry : EJH 54.2 (2010 Apr 15): e19.

    FACS References

    Novel monoclonal antibodies against Pdx1 reveal feedback regulation of Pdx1 protein levels.
    Hald J
    European journal of histochemistry : EJH 54.2 (2010 Apr 15): e19.

    Epitope Map References

    Novel monoclonal antibodies against Pdx1 reveal feedback regulation of Pdx1 protein levels.
    Hald J
    European journal of histochemistry : EJH 54.2 (2010 Apr 15): e19.

    All References

    Novel monoclonal antibodies against Pdx1 reveal feedback regulation of Pdx1 protein levels.
    Hald J
    European journal of histochemistry : EJH 54.2 (2010 Apr 15): e19.

    Sexually dimorphic diet-induced insulin resistance in obese tissue inhibitor of metalloproteinase-2 (TIMP-2)-deficient mice.
    Pratley RE
    Endocrinology 152.4 (2011 Apr): 1300-13.

    Transcriptional dynamics of endodermal organ formation.
    Melton DA
    Developmental dynamics : an official publication of the American Association of Anatomists 238.1 (2009 Jan): 29-42.

    PDX1(LOW) MAFA(LOW) β-cells contribute to islet function and insulin release.
    Hodson DJ
    Nature communications 12.1 (2021 Jan 29): 674.

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