P3H3-Wash

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$45.00 to $425.00
SKU: P3H3-Wash
View product citations for antibody P3H3-Wash on CiteAb

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Available: 52

DSHB Data Sheet

Catalog Fields

Antigen: Wash
Available to For-Profits: Yes
Antigen Species: Drosophila melanogaster
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: Parkhurst, S.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Drosophila
Depositors Institution: Fred Hutchinson Cancer Research Center
Antigen Molecular Weight: Predicted: 53 kDa; Apparent: 75 kDa
Depositors Notes:
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Recombinant Wash/GST fusion protein
Gene: wash
Alternate Antibody Name:
Alternate Gene Names: CG13176
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain:
Epitope Mapped: No
Uniprot ID: Q7JW27 
Epitope Location or Sequence:
Entrez Gene ID: 36311 
Immunogen Sequence: Full length protein
Antibody Registry ID: AB_2618389 
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
Additional Information: wash: Wiskott-Aldrich syndrome protein. Wash polymerizes branched actin through activation of the Arp2/3 complex, as well as bundles and/or crosslinks actin and microtubules.
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P3H3-Wash was deposited to the DSHB by Parkhurst, S. (DSHB Hybridoma Product P3H3-Wash)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

5 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • All References
  • Initial Publication

    Wash functions downstream of Rho and links linear and branched actin nucleation factors.
    Parkhurst SM
    Development (Cambridge, England) 136.16 (2009 Aug): 2849-60.

    IF References

    Wash functions downstream of Rho and links linear and branched actin nucleation factors.
    Parkhurst SM
    Development (Cambridge, England) 136.16 (2009 Aug): 2849-60.

    Arp2/3-mediated F-actin formation controls regulated exocytosis in vivo.
    Ten Hagen KG
    Nature communications 6. (2015 Dec 7): 10098.

    Developmental expression of Drosophila Wiskott-Aldrich Syndrome family proteins.
    Parkhurst SM
    Developmental dynamics : an official publication of the American Association of Anatomists 241.3 (2012 Mar): 608-26.

    The retromer complex is required for rhodopsin recycling and its loss leads to photoreceptor degeneration.
    Bellen HJ
    PLoS biology 12.4 (2014 Apr): e1001847.

    WB References

    Wash functions downstream of Rho and links linear and branched actin nucleation factors.
    Parkhurst SM
    Development (Cambridge, England) 136.16 (2009 Aug): 2849-60.

    Genome-Wide Mapping Targets of the Metazoan Chromatin Remodeling Factor NURF Reveals Nucleosome Remodeling at Enhancers, Core Promoters and Gene Insulators.
    Badenhorst P
    PLoS genetics 12.4 (2016 Apr): e1005969.

    Developmental expression of Drosophila Wiskott-Aldrich Syndrome family proteins.
    Parkhurst SM
    Developmental dynamics : an official publication of the American Association of Anatomists 241.3 (2012 Mar): 608-26.

    IP References
    All References

    Wash functions downstream of Rho and links linear and branched actin nucleation factors.
    Parkhurst SM
    Development (Cambridge, England) 136.16 (2009 Aug): 2849-60.

    Arp2/3-mediated F-actin formation controls regulated exocytosis in vivo.
    Ten Hagen KG
    Nature communications 6. (2015 Dec 7): 10098.

    Developmental expression of Drosophila Wiskott-Aldrich Syndrome family proteins.
    Parkhurst SM
    Developmental dynamics : an official publication of the American Association of Anatomists 241.3 (2012 Mar): 608-26.

    The retromer complex is required for rhodopsin recycling and its loss leads to photoreceptor degeneration.
    Bellen HJ
    PLoS biology 12.4 (2014 Apr): e1001847.

    Genome-Wide Mapping Targets of the Metazoan Chromatin Remodeling Factor NURF Reveals Nucleosome Remodeling at Enhancers, Core Promoters and Gene Insulators.
    Badenhorst P
    PLoS genetics 12.4 (2016 Apr): e1005969.

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