P4C9-Wash

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$40.00
SKU: P4C9-Wash
View product citations for antibody P4C9-Wash on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Wash
Hybridoma Cells Available: Yes
Antigen Species: Drosophila melanogaster
Depositor: Parkhurst, S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Fred Hutchinson Cancer Research Center
Positive Tested Species Reactivity: Drosophila melanogaster
Depositors Notes: Fixation for immunostaining: 4% paraformaldehyde in PBS or standard embryo protocol.
Antigen Molecular Weight: Predicted: 53 kDa; Apparent: 75 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: wash
Immunogen: Recombinant Wash/GST fusion protein
Alternate Gene Names: CG13176
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS-1/FOX-NY
Epitope Location or Sequence:
Uniprot ID: Q7JW27 
Immunogen Sequence: Full length protein
Entrez Gene ID: 36311 
Additional Characterization:  
Antibody Registry ID: AB_2618391 
Additional Information: wash: Wiskott-Aldrich syndrome protein. Wash polymerizes branched actin through activation of the Arp2/3 complex, as well as bundles and/or crosslinks actin and microtubules. P4C9 gives additional bands and doesn't appear to work as well as P3H3 (a different anti-Wash clone in the DSHB collection) for western blotting.
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P4C9-Wash was deposited to the DSHB by Parkhurst, S. (DSHB Hybridoma Product P4C9-Wash)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • WB References
  • All References
  • Initial Publication

    Developmental expression of Drosophila Wiskott-Aldrich Syndrome family proteins.
    Parkhurst SM
    Developmental dynamics : an official publication of the American Association of Anatomists 241.3 (2012 Mar): 608-26.

    WB References

    Developmental expression of Drosophila Wiskott-Aldrich Syndrome family proteins.
    Parkhurst SM
    Developmental dynamics : an official publication of the American Association of Anatomists 241.3 (2012 Mar): 608-26.

    All References

    Developmental expression of Drosophila Wiskott-Aldrich Syndrome family proteins.
    Parkhurst SM
    Developmental dynamics : an official publication of the American Association of Anatomists 241.3 (2012 Mar): 608-26.

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